Article
Biochemistry & Molecular Biology
N. Balneger, L. A. M. Cornelissen, M. Wassink, S. J. Moons, T. J. Boltje, Y. E. Bar-Ephraim, K. K. Das, J. N. Sondergaard, C. Bull, G. J. Adema
Summary: The study reveals that sialic acids play a crucial role in regulating the activation and interaction of dendritic cells. Blocking sialic acids enhances the immune activation ability and antigen-induced T cell interactions of dendritic cells. These findings have important implications for understanding the mechanisms of immune responses.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Michal A. Stanczak, Natalia Rodrigues Mantuano, Nicole Kirchhammer, David E. Sanin, Francis Jacob, Ricardo Coelho, Arun V. Everest-Dass, Jinyu Wang, Marcel P. Trefny, Gianni Monaco, Anne Barenwaldt, Melissa A. Gray, Adam Petrone, Abhishek S. Kashyap, Katharina Glatz, Benjamin Kasenda, Karl Normington, James Broderick, Li Peng, Oliver M. T. Pearce, Erika L. Pearce, Carolyn R. Bertozzi, Alfred Zippelius, Heinz Laubli
Summary: This study demonstrates the correlation between tumor sialylation and immune states as well as survival, and proposes therapeutic desialylation as an approach to enhance antitumor immunity. The study also reveals the impact of desialylation on tumor-associated macrophages and the efficacy of immune checkpoint blockade.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biochemistry & Molecular Biology
Yu Zhou, Su-Hyun Park, Nam -Hai Chua
Summary: Salicylic acid (SA) is a critical defense hormone for plant immunity. NPR1 and its paralogs NPR3 and NPR4 play important roles in mediating SA signal transduction. This study reveals that UBP12 and UBP13 negatively regulate plant immunity by promoting the stability of NPR3 and NPR4 through deubiquitination.
Review
Oncology
Xiuting Liu, Graham D. Hogg, David G. DeNardo
Summary: The success of checkpoint inhibitors in cancer treatment has highlighted the importance of the immune system, particularly in eliciting antitumor responses. While initially thought to primarily target T cells, recent research suggests that these inhibitors may also impact innate immunity, thus playing a significant role in clinical efficacy. Further investigation into the mechanisms of action of checkpoint therapies on innate antitumor responses is crucial for developing effective combination treatments to combat checkpoint resistance in refractory malignancies.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Pharmacology & Pharmacy
Meng Chen, Zhaowei Qi, Xianmin Meng, Shuo Wang, Xueying Zheng, Miao Hu, Xinrong Liu, Yanzhi Song, Yihui Deng
Summary: Neutralizing the inhibitory effect of neutrophils on T cells can enhance the effectiveness of immunotherapy and increase the sensitivity of anti-tumor drugs and immune checkpoint inhibitors.
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Chemistry, Medicinal
Jeong Hyeon Cha, Eunsu Kim, Hyeong Ji Lee, Young-Ho Lee, Jeonghyun Lee, Eunha Kim, Chan Hyuk Kim
Summary: Metabolic glycan labeling combined with CAR-T cell therapy can provide novel cancer immunotherapy for solid tumors that lack viable target antigens.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Immunology
Yonghua Cai, Ke Li, Jie Lin, Xianqiu Liang, Wei Xu, Zhengming Zhan, Shuaishuai Xue, Yu Zeng, Peng Chai, Yangqi Mao, Zibin Song, Lei Han, Ye Song, Xian Zhang, Hai Wang
Summary: This study explores the role of pyroptosis in glioma and identifies three pyroptosis subtypes with different prognoses. It also finds that patients with high gasdermin-related prognostic index (GPI) are more sensitive to certain treatments. Gasdermin D is identified as a potential biomarker in pyroptosis-inducible therapy combined with immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Ingunn M. Stromnes, Ayaka Hulbert, Meagan R. Rollins, Ryan S. Basom, Jeffrey Delrow, Patrick Bonson, Adam L. Burrack, Sunil R. Hingorani, Philip D. Greenberg
Summary: This study investigates the role of immune checkpoints in mediating functional dysfunction of engineered T cells in pancreatic ductal adenocarcinoma (PDA). The findings suggest that blockade of PD-1 signaling alone is not enough to overcome the dysfunction of TCR engineered T cells in PDA. Contributions from both the differentiation pathways induced during the T cell engineering process and intratumoral suppressive mechanisms render engineered T cells dysfunctional.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Medicine, Research & Experimental
Dezhi Sui, Kaifan Liang, Yangxu Gui, Zhouchunxiao Du, Dongzhe Xin, Guojing Yu, Wantong Zhai, Xinrong Liu, Yanzhi Song, Yihui Deng
Summary: This study investigated the effects of SA derivatives on drug delivery systems, synthesizing four different SA derivatives and preparing SA derivative-modified liposomes. The results showed that an SA-cholesterol conjugate improved liposome stability, reduced plasma protein adsorption, and enhanced targeting of TAMs, leading to increased infiltration of CD8+ T cells in the tumor microenvironment. The findings provide valuable insights for research and development on SA-derivatives and offer a theoretical basis for clinical trials of SA-derivative-modified nanoparticles.
Article
Infectious Diseases
Yi-Jung Li, Jiun-Wen Wang, Hsin-Hsu Wu, Hsu-Han Wang, Yang-Jen Chiang, Huang-Yu Yang, Hsiang-Hao Hsu, Chih-Wei Yang, Ya-Chung Tian
Summary: Inhibition of TNF-alpha can attenuate the inflammatory response and viral replication induced by BKPyV infection, providing a potential therapeutic target for BKPyV-associated kidney damage.
Review
Pharmacology & Pharmacy
Guangna Liu, Motao Zhu, Xiao Zhao, Guangjun Nie
Summary: Vaccination has become an effective method to stimulate immunity for disease prevention and treatment, but a tailored efficient delivery system for specific antigens is still urgently needed, especially for vaccines targeting CD8(+) T cells-mediated cellular immunity. Nanotechnology plays a crucial role in enhancing antigen stability and immunogenicity, as well as facilitating APCs-targeted delivery and increased cross-presentation for effective immune protection and clearance.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Medicine, Research & Experimental
Cong Li, Chuizhong Fan, Shuang Lu, Qiujun Qiu, Xin Gao, Xinyang Yan, Shuo Wang, Bing Zhao, Xinrong Liu, Yanzhi Song, Yihui Deng
Summary: Immune checkpoint blockade therapy has shown promise, but enhancing the proportion of patients benefiting from the therapy remains challenging. This study presents a nanocomplex modified with a sialic acid derivative that improves the efficacy of a tyrosine kinase inhibitor in solid tumors. Combining this nanocomplex with PD-L1 blockade agents effectively suppresses tumor growth.
MOLECULAR PHARMACEUTICS
(2023)
Article
Multidisciplinary Sciences
Alba Rodriguez-Garcia, Rachel C. Lynn, Mathilde Poussin, Monika A. Eiva, Lauren C. Shaw, Roddy S. O'Connor, Nicholas G. Minutolo, Victoria Casado-Medrano, Gonzalo Lopez, Takami Matsuyama, Daniel J. Powell
Summary: The study demonstrates that targeting FR beta-expressing TAMs with CAR-T cells can enhance antitumor immune responses and improve the efficacy of adoptive T-cell therapy in pre-clinical models.
NATURE COMMUNICATIONS
(2021)
Review
Immunology
Zhengguo Wu, Shang Li, Xiao Zhu
Summary: Cancer immunotherapy is a promising treatment to control and eliminate tumors, but it is currently only effective for certain types of tumors and faces challenges. By studying tumor microenvironment immunotherapy, the anti-tumor immunity can be enhanced, improving the effectiveness of immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sam J. Moons, Emiel Rossing, Mathilde A. C. H. Janssen, Torben Heise, Christian Bull, Gosse J. Adema, Thomas J. Boltje
Summary: Sialic acids play a protective role on the glycans of cell surface glycoproteins and glycolipids and are associated with pathologies such as cancer. Inhibiting sialic acid biosynthesis can be used for anticancer therapy. Modifying the C-S and C-5 positions of 3-fluorosialic acid derivatives enhances their inhibitory potency and can improve the efficiency of sialic acid metabolic labeling reagents.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
N. Balneger, L. A. M. Cornelissen, M. Wassink, S. J. Moons, T. J. Boltje, Y. E. Bar-Ephraim, K. K. Das, J. N. Sondergaard, C. Bull, G. J. Adema
Summary: The study reveals that sialic acids play a crucial role in regulating the activation and interaction of dendritic cells. Blocking sialic acids enhances the immune activation ability and antigen-induced T cell interactions of dendritic cells. These findings have important implications for understanding the mechanisms of immune responses.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biology
Riem Gawish, Philipp Starkl, Lisabeth Pimenov, Anastasiya Hladik, Karin Lakovits, Felicitas Oberndorfer, Shane J. F. Cronin, Anna Ohradanova-Repic, Gerald Wirnsberger, Benedikt Agerer, Lukas Endler, Tumay Capraz, Jan W. Perthold, Domagoj Cikes, Rubina Koglgruber, Astrid Hagelkruys, Nuria Montserrat, Ali Mirazimi, Louis Boon, Hannes Stockinger, Andreas Bergthaler, Chris Oostenbrink, Josef M. Penninger, Sylvia Knapp
Summary: Despite progress in understanding COVID-19, limited mechanistic insight into immunological factors and disease severity remains. Scientists generated a mouse-adapted SARS-CoV-2 strain, maVie16, and found that cytokine-driven immunopathology and ACE2 dependence determine disease severity. This study provides a new tool for discovering therapies.
Article
Cell Biology
Gaurang Jhala, Balasubramanian Krishnamurthy, Thomas C. Brodnicki, Tingting Ge, Satoru Akazawa, Claudia Selck, Prerak M. Trivedi, Evan G. Pappas, Leanne Mackin, Nicola Principe, Erwan Bremaud, David J. De George, Louis Boon, Ian Smyth, Jonathan Chee, Thomas W. H. Kay, Helen E. Thomas
Summary: IFN gamma regulates the expansion of antigen-specific CD8(+) T cells by controlling the expression of SOCS-1. Despite reduced inflammation in Ifngr-mutant mice, the expanded CD8(+) T cells may contribute to normal diabetes progression.
Article
Immunology
Nicola Principe, Wayne J. Aston, Danika E. Hope, Caitlin M. Tilsed, Scott A. Fisher, Louis Boon, Ian M. Dick, Wee Loong Chin, Alison M. McDonnell, Anna K. Nowak, Richard A. Lake, Jonathan Chee, Willem Joost Lesterhuis
Summary: This study investigated the combination effects of various chemotherapies with immune checkpoint blockade (ICB) and identified chemotherapy/ICB combinations that showed additive or non-additive effects. The combination of 5-fluorouracil (5-FU) or cisplatin with ICB had additive effects, while vinorelbine and etoposide did not provide additional benefit when combined with ICB. Furthermore, the study found that the combination of 5-FU with ICB increased inflammation in the tumor microenvironment, which may contribute to its effective combination therapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Peter Rose, Natasja K. van den Engel, Julia R. Kovacs, Rudolf A. Hatz, Louis Boon, Hauke Winter
Summary: Depleting antibodies targeting myeloid-derived suppressor cells (MDSC) enhance the induction of tumor-specific T cells and delay the growth of melanoma.
Article
Immunology
Marco Gargaro, Giulia Scalisi, Giorgia Manni, Carlos G. Briseno, Prachi Bagadia, Vivek Durai, Derek J. Theisen, Sunkyung Kim, Marilena Castelli, Chenling A. Xu, Gerd Meyer zu Horste, Giuseppe Servillo, Maria A. Della Fazia, Giulia Mencarelli, Doriana Ricciuti, Eleonora Padiglioni, Nicola Giacche, Carolina Colliva, Roberto Pellicciari, Mario Calvitti, Teresa Zelante, Dietmar Fuchs, Ciriana Orabona, Louis Boon, Alban Bessede, Marco Colonna, Paolo Puccetti, Theresa L. Murphy, Kenneth M. Murphy, Francesca Fallarino
Summary: This study identifies a previously unrecognized metabolic communication pathway, in which cDC1 cells extend their immunoregulatory capacity to cDC2 through the production of L-kynurenine. This finding has potential therapeutic implications in treating autoimmune demyelinating diseases.
Article
Biochemistry & Molecular Biology
Dirk J. W. den Braanker, Rutger J. H. Maas, Guido van Mierlo, Naomi M. J. Parr, Marinka Bakker-van Bebber, Jeroen K. J. Deegens, Pascal W. T. C. Jansen, Jolein Gloerich, Brigith Willemsen, Henry B. B. Dijkman, Alain J. J. van Gool, Jack F. M. Wetzels, Markus M. M. Rinschen, Michiel Vermeulen, Tom Nijenhuis, Johan van der Vlag
Summary: This study aimed to investigate the possible factors and mechanisms leading to recurrence of focal segmental glomerulosclerosis (FSGS) after kidney transplantation. It was found that a protein called perilipin-2 was significantly increased in infiltrating cells of patients' glomeruli and was associated with lipid droplet accumulation, suggesting it as a potential biomarker. Further research should focus on understanding how these factors alter lipid metabolism in podocytes and provide new treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Jennifer Eymael, Martijn van den Broek, Laura Miesen, Valerie Villacorta Monge, Bartholomeus T. van den Berge, Fieke Mooren, Vicky Luna Velez, Jelmer Dijkstra, Meyke Hermsen, Peter Bandi, Michiel Vermeulen, Saskia de Wildt, Brigith Willemsen, Sandrine Florquin, Roy Wetzels, Eric Steenbergen, Rafael Kramann, Marcus Moeller, Michiel F. Schreuder, Jack Fm Wetzels, Johan van der Vlag, Jitske Jansen, Bart Smeets
Summary: Scattered tubular cells (STCs) in the proximal tubule increase in number after acute kidney injury. STCs are preferentially located within the inner bends of the tubule and their number increases with age. STCs represent a transient state of dedifferentiated proximal tubule epithelial cells (PTECs) showing metabolic shift towards glycolysis, facilitating cellular survival after kidney injury.
JOURNAL OF PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike W. Looman, Erik Hooijberg, Christian Bull, Daniel Granado, Lenneke A. M. Cornelissen, Gosse J. Adema
Summary: Tumors create an immunosuppressive tumor microenvironment by altering protein expression and changing their glycosylation status, like altered expression of sialoglycans. Siglec-7, an inhibitory immune receptor similar to PD-1, is used by cancer cells as a glycoimmune checkpoint to evade the immune system. The exact cellular and molecular conditions required for Siglec-7-mediated immune cell inhibition remain largely unknown.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Microbiology
Katrien C. K. Poelaert, Regina G. D. M. van Kleef, Mengying Liu, Arno van Vliet, Heyrhyoung Lyoo, Lora-Sophie Gerber, Yoshiki Narimatsu, Christian Buell, Henrik Clausen, Erik de Vries, Remco H. S. Westerink, Frank J. M. van Kuppeveld
Summary: Enterovirus D68 (EV-D68) is an emerging pathogen associated with mild to severe respiratory disease and acute flaccid myelitis (AFM). This study explores the entry, replication, and functional consequences of historical and contemporary EV-D68 strains in primary rat cortical neurons. The study reveals the importance of sialic acids as receptors for infection of neurons and respiratory epithelial cells. It also shows that both excitatory glutamatergic and inhibitory GABA-ergic neurons can be infected by EV-D68.
Article
Biology
Melina Hussmann, Dorte Schulte, Sarah Weischer, Claudia Carlantoni, Hiroyuki Nakajima, Naoki Mochizuki, Didier Y. R. Stainier, Thomas Zobel, Manuel Koch, Stefan Schulte-Merker, Victoria L. Bautch
Summary: This study reveals the critical roles of Svep1 and Tie1 in the development of specific subpopulations of the zebrafish facial lymphatic network. It also shows that this aspect of the network is formed independently of Vegfc signaling. The findings demonstrate the importance of Tie1 signaling in lymphangiogenesis and blood vessel development in zebrafish.
Article
Biochemical Research Methods
Daniel L. A. H. Hornikx, Eline A. Visser, Venetia Psomiadou, Christian Bull, Thomas J. Boltje
Summary: This article presents a protocol for detecting and quantifying sialic acid mimetics (SAMs) using microscopy and flow cytometry. The steps for linking SAMs to proteins with western blotting are detailed. Additionally, the article explains the procedures for incorporative or inhibitory SAMs and how SAMs can be used for the on-cell synthesis of high-affinity Siglec ligands.
Article
Biochemistry & Molecular Biology
Sam J. Moons, Daniel L. A. H. Hornikx, Mikkel K. M. Aasted, Johan F. A. Pijnenborg, Matteo Calzari, Paul B. White, Yoshiki Narimatsu, Henrik Clausen, Hans H. Wandall, Thomas J. Boltje, Christian Buell
Summary: This passage describes the role of sialic acids in mammalian glycoproteins and glycolipids, and their importance and applications in diseases such as cancer and infections. Researchers have developed a photosensitive inhibitor that can be selectively activated with UV light, allowing for local inhibition of sialic acid synthesis.
RSC CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Monique van Scherpenzeel, Federica Conte, Christian Bull, Angel Ashikov, Esther Hermans, Anke Willems, Walinka van Tol, Else Kragt, Marek Noga, Ed E. Moret, Torben Heise, Jeroen D. Langereis, Emiel Rossing, Michael Zimmermann, M. Estela Rubio-Gozalbo, Marien de Jonge, Gosse J. Adema, Nicola Zamboni, Thomas Boltje, Dirk J. Lefeber
Summary: This study used ion-pair ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry to analyze sugar metabolites in cells and organisms and identified low abundant nucleotide sugars. Time-course analysis of the effect of an antitumor compound revealed full depletion of specific sugar metabolites within 24 hours.