Article
Biochemistry & Molecular Biology
Boglarka Zambo, Gergo Gogl, Bastien Morlet, Pascal Eberling, Luc Negroni, Herve Moine, Gilles Trave
Summary: This study comprehensively investigates the PDZ-PBM interactome of diacylglycerol kinases (DGKs) using interactomic approaches and evolutionary analysis, revealing functional differences between PBMs of different types of DGKs.
Article
Multidisciplinary Sciences
Louise Laursen, Raviteja Inturi, Soren Ostergaard, Per Jemth
Summary: This study investigated the interaction between the PSG supramodule and other proteins in the post-synaptic density using bioinformatics and biophysical methods. The results showed that the PDZ3 domain had specific binding with peptides, and mutations in PDZ3 could affect the affinity and specificity of the interaction. Additionally, the study found that the ADGRB1 protein formed liquid droplets with the PSG supramodule, providing new insights into PSD formation.
Article
Multidisciplinary Sciences
Sachiko Fujiwara, Thanh Phuong Nguyen, Kyoko Furuse, Yugo Fukazawa, Tetsuhisa Otani, Mikio Furuse
Summary: The interaction of claudins with ZO proteins is not a prerequisite for tight junction formation at the most apical part of cell-cell contacts.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Krystyna A. Gieniec, Felicity M. Davis
Summary: Lactation is crucial for the survival and reproduction of mammals, but the mechanisms of milk production and ejection remain poorly understood. This review focuses on how basal cells contract to expel milk and discusses the coordination of these cells, their fate after lactation, and potential avenues for further research and exploration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
A-Reum Nam, Min Heo, Kang-Hoon Lee, Ji-Yoon Kim, Sung-Ho Won, Je-Yoel Cho
Summary: In this study, the differential DNA methylome landscapes of peripheral blood mononuclear cells (PBMCs) from 76 canines were characterized using methylated CpG-binding domain sequencing (MBD-seq). Genes involved in the growth and differentiation of T- and B-cells were found to be highly methylated in tumor PBMCs. The study also revealed increased methylation and reversed expression in marker genes regulating immune cell proliferation, and successfully differentiated between benign and malignant tumors and the presence of mammary gland tumors using the PBMC methylome through a machine-learning approach.
Review
Oncology
Xinyuan Jiang, Zhiyong Xu, Sujing Jiang, Huan Wang, Mingshu Xiao, Yueli Shi, Kai Wang
Summary: PDZ-LIM family proteins are scaffolding proteins that contain PDZ and LIM interaction domains. The PDZ and LIM domains function as scaffolds to bind to various proteins. PDLIMs are evolutionarily conserved proteins found in different species. They mediate the interaction of signal molecules and are involved in important physiological processes such as cell differentiation, proliferation, migration, and maintenance of cellular structural integrity. Dysregulation of PDLIMs has been linked to tumor formation and development. This paper reviews and integrates the current knowledge on PDLIMs, describing the structure and function of PDZ and LIM domains and the role of PDLIMs in tumor development.
Article
Multidisciplinary Sciences
Chisaki Ikeda, Hiroaki Haga, Naohiko Makino, Tatsutoshi Inuzuka, Ayako Kurimoto, Toshiki Ueda, Akiko Matsuda, Yasuharu Kakizaki, Tetsuya Ishizawa, Toshikazu Kobayashi, Shinpei Sugahara, Michihiko Tsunoda, Kensei Suda, Yoshiyuki Ueno
Summary: This study isolated EVs from human bile using EDEG and performed proteomic analysis, identifying Claudin-3 as a useful biomarker for CCA. The diagnostic accuracy was high with AUC 0.945 and sensitivity and specificity of 87.5%.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Holly Holliday, Daniel Roden, Simon Junankar, Sunny Z. Wu, Laura A. Baker, Christoph Krisp, Chia-Ling Chan, Andrea McFarland, Joanna N. Skhinas, Thomas R. Cox, Bhupinder Pal, Nicholas D. Huntington, Christopher J. Ormandy, Jason S. Carroll, Jane Visvader, Mark P. Molloy, Alexander Swarbrick
Summary: ID4 functions as a repressor of myoepithelial differentiation in mammary basal cells by regulating gene expression, antagonizing HEB transcriptional activity, and promoting appropriate tissue morphogenesis. During pregnancy, downregulation of ID4 modulates genes regulated by HEB, promoting specialization of basal cells into myoepithelial cells.
Article
Biochemistry & Molecular Biology
Leire Torices, Janire Mingo, Isabel Rodriguez-Escudero, Teresa Fernandez-Acero, Sandra Luna, Caroline E. Nunes-Xavier, Jose Lopez, Fatima Mercadillo, Maria Curras, Miguel Urioste, Maria Molina, Victor J. Cid, Rafael Pulido
Summary: Heterozygous germline mutations in PTEN gene are associated with hamartomas, tumors, and neurodevelopmental disorders. PTEN acts as a phosphatase, counteracting the pro-oncogenic function of PI3K. This study characterized several PTEN variants found in PHTS patients and observed complex patterns of loss of function, altered localization, and altered cleavage by caspase-3.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Virology
Celia Caillet-Saguy, Nicolas Wolff
Summary: ACE2 is a main receptor for SARS-CoV-2 entry to the host cell, and its interaction with PDZ could facilitate virus entry. The study identified potential interactions between ACE2 and neuronal proteins, which may be involved in the neurological symptoms of COVID-19.
Article
Biochemistry & Molecular Biology
Mariano Martin, Lisa Salleron, Victoria Peyret, Romina Celeste Geysels, Elisabeth Darrouzet, Sabine Lindenthal, Carlos Eduardo Bernal Barquero, Ana Maria Masini-Repiso, Thierry Pourcher, Juan Pablo Nicola
Summary: NIS gene mutations leading to impaired iodide accumulation cause congenital hypothyroidism, as demonstrated by a truncated NIS protein missing some amino acids mislocalized into intracellular vesicles. Further investigation reveals the involvement of the PDZ domain in the interaction between the protein SCRIB and NIS, affecting the plasma membrane localization of NIS.
Article
Biochemistry & Molecular Biology
Eva S. Cobos, Ignacio E. Sanchez, Lucia B. Chemes, Jose C. Martinez, Javier Murciano-Calles
Summary: PDZ domains play a crucial role in cell signaling and cell-cell junctions, being able to recognize various natural targets. The study reveals a basic specificity in each PDZ domain, with host partners mostly being able to bind their corresponding PDZ domain. Additionally, viral ligands show the ability to bind most of the studied PDZ domains, including those not previously described.
Article
Biochemistry & Molecular Biology
Zi-Lin Li, Chen-Yu Gou, Wen-Hui Wang, Yuan Li, Yu Cui, Jing-Jing Duan, Yuan Chen
Summary: Nicotinic acetylcholine receptors (nAChRs) are regulated by PDZ-LIM domain protein PDLIM5, which interacts with alpha 7AChRs and contributes to their upregulation and surface expression. This interaction is mediated through the PDZ domain, and can be promoted by nicotine.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Pathology
Joanna K. M. Ng, Joshua J. X. Li, Billy S. W. Lai, Julia Y. Tsang, Agnes W. S. Chan, Christina M. T. Cheung, Edric C. C. Ip, Gary M. Tse
Summary: WT1 immunostain is expressed in various benign and malignant neoplasms, as well as normal myoepithelial cells. The expression profile of WT1 differs in non-neoplastic, benign, and malignant neoplastic myoepithelial cells of the salivary gland. WT1 is a less sensitive myoepithelial marker than calponin and p63, but its unique expression pattern in salivary gland primary for pleomorphic adenomas/cutaneous mixed tumors can aid in the diagnosis of benign salivary gland tumors.
Article
Anatomy & Morphology
R. S. N. Barreto, H. J. C. Carvalho, G. S. S. Matias, M. G. K. C. Silva, R. R. Ribeiro, T. B. Campanelli, N. N. Rigoglio, A. C. O. Carreira, M. A. Miglino
Summary: Extracellular matrix (ECM) proteins play a crucial role in the structure and regulation of the mammary gland. Alterations in the ECM can affect the development of breast tumors. Through immunohistochemistry and decellularization, the protein profile of healthy and tumoral canine mammary ECM was studied. The study also investigated the influence of the ECM on cell attachment in both healthy and tumoral conditions.
Article
Health Care Sciences & Services
Fraide A. Ganotice, Amy Yin Man Chow, Kelvin Kai Hin Fan, Ui Soon Khoo, May Pui San Lam, Rebecca Po Wah Poon, Francis Hang Sang Tsoi, Michael Ning Wang, George L. Tipoe
Summary: The study showed that the Interprofessional Attitude Scale (IPAS) had good internal consistency and most of the confirmatory factor analysis results supported the overall five-factor solution. The various subscales of IPAS systematically correlated with other theoretically relevant variables such as teamwork attitudes, communication, and team effectiveness.
JOURNAL OF INTERPROFESSIONAL CARE
(2022)
Review
Biochemistry & Molecular Biology
Chan-Ping You, Man-Hong Leung, Wai-Chung Tsang, Ui-Soon Khoo, Ho Tsoi
Summary: Biomarkers, such as estrogen receptor alpha(ER alpha), human epidermal growth factor receptor 2 (HER2), and androgen receptor (AR), have been widely used in breast cancer diagnosis, prognosis, and prediction. AR serves different roles in different breast cancer subtypes, acting as a tumor suppressor in ER+ breast cancers but a tumor promoter in ER- breast cancers. AR expression status can also predict the response to hormonal therapy and chemotherapy. Therefore, AR has the potential to be a useful biomarker in breast cancer.
Article
Biology
Ho Tsoi, Ling Shi, Man-Hong Leung, Ellen P. S. Man, Zi-Qing So, Wing-Lok Chan, Ui-Soon Khoo
Summary: A splice variant of NCOR2, called BQ, enhances tamoxifen resistance in ER+ breast cancer cells by modulating IL-8 expression. Targeting IL-8 signaling pathway is a promising approach to overcome tamoxifen resistance in ER+ breast cancer.
Article
Pathology
Mingfei Yan, Ruba Khattab, Howard Meyerson
Summary: We report a rare case of ALK-positive large B cell lymphoma initially presenting as a breast lesion. Immunophenotypic and immunostaining results revealed typical immunoblastic morphology and confirmed the fusion of ALK and CTCL. Additionally, expression of CD10 and BCL6 suggested a germinal center origin for the lesion.
INTERNATIONAL JOURNAL OF SURGICAL PATHOLOGY
(2023)
Article
Education, Scientific Disciplines
Fraide A. Ganotice, Linda Chan, Amy Yin Man Chow, Ui Soon Khoo, May Pui San Lam, Rebecca Ka Wai Liu, Rebecca Po Wah Poon, Michael Ning Wang, Francis Hang Sang Tsoi, George L. Tipoe
Summary: This study investigated the differences in attitudes and achievement between high-and low-performing teams in an online asynchronous and synchronous IPE program, and the role of autonomous motivation in determining team membership. High-performing teams were characterized by their recognition and endorsement of important interprofessional collaborative competencies, which was predicted by autonomous motivation.
NURSE EDUCATION TODAY
(2022)
Article
Oncology
Ho Tsoi, Chan-Ping You, Man-Hong Leung, Ellen P. S. Man, Ui-Soon Khoo
Summary: Breast cancer is a heterogeneous disease, and most cases are estrogen receptor-positive. However, many patients eventually develop resistance to the main treatment drug tamoxifen. Overexpression of c-MYC can drive the development of estrogen receptor-positive breast cancer and confer tamoxifen resistance. Ribosome biogenesis plays a crucial role in this process, and suppressing ribosome biogenesis may help reduce aggressiveness and reverse tamoxifen resistance. Some chemicals have been shown to repress ribosome biogenesis and may potentially reverse tamoxifen resistance in estrogen receptor-positive breast cancer. Identification of predictive markers will be necessary for the future use of these ribosome biogenesis inhibitors in combating tamoxifen resistance.
Article
Pharmacology & Pharmacy
Huanyu Ding, Minchun Jiang, Chi Wai Lau, Jianfang Luo, Andrew M. M. Chan, Li Wang, Yu Huang
Summary: YAP inhibition by CBL0137 is a promising therapeutic approach for atherosclerotic vascular disease.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Pathology
Mingfei Yan, Howard Meyerson, Kwadwo A. A. Oduro, Shashirekha Shetty, Jennifer Yoest
Summary: This study reports an unusual MYD88 in-frame deletion in an aggressive lymphoplasmacytic neoplasm. Only 8 other mature B-cell lymphomas with MYD88 in-frame deletions were identified, but none of them were diagnosed with lymphoplasmacytic lymphoma. Recognizing such in-frame deletions is necessary to understand the mutational spectrum of MYD88 in B-cell lymphomas, and further investigation is needed to determine whether these deletions are overrepresented in lymphoplasmacytic lymphoma compared to other B-cell lymphomas.
INTERNATIONAL JOURNAL OF SURGICAL PATHOLOGY
(2023)
Article
Pathology
Mingfei Yan, Jing Wang, Ramya Gadde, Philip Bomeisl, Hannah Gilmore, Aparna Harbhajanka
Summary: This study analyzed the clinical and pathological features of 5 primary and 6 secondary breast MALT lymphomas diagnosed in our institution over a 20-year period. Most primary and secondary breast MALT lymphomas had similar clinical presentations, but primary lymphomas tended to occur in older patients and thyroid abnormalities were common. There were no distinct histopathologic findings in primary lymphomas, indicating that they do not share the distinctive features of primary cutaneous marginal zone lymphoma.
INTERNATIONAL JOURNAL OF SURGICAL PATHOLOGY
(2023)
Article
Multidisciplinary Sciences
Stanley K. K. Cheung, Jacinda Kwok, Penelope M. Y. Or, Chi Wai Wong, Bo Feng, Kwong Wai Choy, Raymond C. C. Chang, J. Peter H. Burbach, Alfred S. L. Cheng, Andrew M. Chan
Summary: PTEN hamartoma tumour syndrome results from mutations in the human PTEN gene. Transcriptomic and proteomic analyses of neural tissues and primary cultures from Pten-knockout mice revealed enrichment in immune response, oligodendrocyte development, and various other cellular processes. The findings suggest potential associations with intelligence, cognitive function, schizophrenia, and depression.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Ho Tsoi, Nicholas Nok-Ching Fung, Ellen P. S. Man, Man-Hong Leung, Chan-Ping You, Wing-Lok Chan, Sum-Yin Chan, Ui-Soon Khoo
Summary: Overexpressing BQ can confer tamoxifen resistance in ER +ve breast cancer. BQ is an alternative splice variant of NCOR2 generated through the exclusion of exon 11. SRSF5 is involved in the regulation of NCOR2 splicing. Low expression of SRSF5 increases the production of BQ mRNA by excluding exon 11. Thus, targeting the upstream pathway of BQ may reverse tamoxifen resistance.
Article
Pathology
Mingfei Yan, Rose C. Beck, Ramya Gadde
Summary: This study aimed to investigate laboratory and bone marrow findings that can predict a diagnosis of HLH in patients with HPC in the bone marrow. The study found that hypertriglyceridemia and more frequent gHPC ingesting granulocytic cells were associated with a higher likelihood of HLH diagnosis.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Oncology
Ho Tsoi, Johann Lok, Ellen P. S. Man, Cheuk-Nam Cheng, Man-Hong Leung, Chan-Ping You, Sum-Yin Chan, Wing-Lok Chan, Ui-Soon Khoo
Summary: This study revealed that overexpression of a novel splice variant BQ323636.1 is associated with resistance to the non-steroidal aromatase inhibitor anastrozole in ER+ post-menopausal breast cancer. Mechanistic studies showed that BQ overexpression enhances androgen receptor (AR) activity, leading to hyper-activation of AR signaling and increased cell proliferation. Targeting AR can overcome anastrozole resistance in breast cancer with BQ overexpression. Clinical study also demonstrated that high nuclear expression of both BQ and AR is significantly associated with poor survival in non-steroidal aromatase inhibitor-treated ER+ breast cancer patients.
JOURNAL OF PATHOLOGY
(2023)
Meeting Abstract
Pathology
Mingfei Yan, Jing Wang, Hannah Gilmore, Philip Bomeisl, Aparna Harbhajanka
Meeting Abstract
Pathology
Mingfei Yan, Ramya Gadde, Rose Beck
Review
Oncology
Xinru Zhou, Yin Jia, Chuanbin Mao, Shanrong Liu
Summary: Small extracellular vesicles (sEVs), such as exosomes, have emerged as crucial targets for liquid biopsy and promising drug delivery vehicles in tumor progression. They can serve as biomarkers for tumor diagnosis and as drug carriers for cancer treatment.
Article
Oncology
Ruochan Chen, Ju Zhu, Xiao Zhong, Jie Li, Rui Kang, Daolin Tang
Summary: The interplay between autophagy and apoptosis plays a crucial role in tumorigenesis and cancer therapy, with HMGB1 serving as a key regulator in these processes.
Article
Oncology
Zongfu Pan, Xixuan Lu, Tong Xu, Jinming Chen, Lisha Bao, Ying Li, Yingying Gong, Yulu Che, Xiaozhou Zou, Zhuo Tan, Ping Huang, Minghua Ge
Summary: This study uncovered the emerging role of HN1 in promoting dedifferentiation of anaplastic thyroid cancer (ATC) cells. HN1 negatively regulated the thyroid differentiation markers and had an inhibitory effect on the transcriptional activation of CTCF, thereby influencing the chromatin accessibility of thyroid differentiation genes.
Article
Oncology
Yi Qin, Shengjun Xiong, Jun Ren, Gautam Sethi
Summary: Autophagy plays an important regulatory role in glioblastoma, and its dysregulation can lead to drug resistance and radioresistance. It also affects stem cell characteristics, overall growth, and metastasis. Therefore, autophagy is a promising target for glioblastoma therapy.
Article
Oncology
Katsuya Nagaoka, Xuewei Bai, Dan Liu, Kevin Cao, Joud Mulla, Chengcheng Ji, Hongze Chen, Muhammad Azhar Nisar, Amalia Bay, William Mueller, Grace Hildebrand, Jin-Song Gao, Shaolei Lu, Hiroko Setoyama, Yasuhito Tanaka, Jack R. Wands, Chiung-Kuei Huang
Summary: This study found that serum 2-OG levels in cholangiocarcinoma patients are associated with the effectiveness of chemotherapy. Patients with progressive disease showed significantly higher levels of serum 2-OG compared to stable disease and partial response patients. The study also revealed that overexpression of ASPH mimics the effects of 2-OG, and knockdown of ASPH improves chemotherapy. Targeting ASPH enhances the effects of chemotherapy by modulating ATM and ATR, two key regulators of DDRs.