Article
Biochemistry & Molecular Biology
Jing Wang, Chunjun Qin, Yujuan Xu, Jian Yin, Jing Hu, Xi Guo
Summary: In this study, the structure of a novel O-antigen from Escherichia coli strain SD2019180 was established, and its role in O-antigen synthesis was confirmed through experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jing Wang, Yujuan Xu, Chunjun Qin, Jing Hu, Jian Yin, Xi Guo
Summary: The study identified the structure of the O-antigen in Escherichia coli strain LL004, showing significant differences from traditional E. coli serogroups, indicating it belongs to a novel serogroup. The O-antigen biosynthesis of LL004 was confirmed to be assembled via the Wzx/Wzy dependent pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chunjun Qin, Bin Hu, Yujuan Xu, Changqing Zhao, Wei Hao, Jing Wang, Xi Guo, Renpeng Li, Jing Hu, Jian Yin
Summary: The O-antigen is an important virulence factor involved in bacterial survival, virulence, and invasion. This study elucidates the structure of O-antigen from an Escherichia coli strain 2017LL031, which is completely unique compared to known E. coli serogroups. The analysis of the O-antigen gene cluster confirms its correlation to the O-antigen structure. Furthermore, experimental evidence confirms the role of the O-antigen gene cluster in O-antigen biosynthesis. Overall, a novel E. coli serotype, 2017LL031, is identified.
CARBOHYDRATE RESEARCH
(2022)
Article
Food Science & Technology
Renpeng Li, Jing Wang, Lu Wang, Bin Hu, Zongdong Liu, Na Sun, Ying Yang, Song Miao, Xi Guo
Summary: In this study, nine untypeable K antigen strains of Vibrio parahaemolyticus were identified and characterized during a serotyping survey in Shandong province, China. Among these strains, three novel K serotypes were identified, with seven possessing the KUT2 antigens reported recently by another group. Comparative genomic analysis showed that the Shandong KUT2 strains exhibit different virulence profiles compared to their identical K serotype partners from Zhejiang province, suggesting possible evolution from a common ancestor. One isolate, SD2016062, was found to be phylogenetically similar to strains associated with local gastroenteritis outbreaks, indicating potential for disease spread. A sero-specific PCR assay targeting the three novel K serotypes was developed for clinical and epidemiological monitoring of V. parahaemolyticus strains.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jing Wang, HongBo Jiao, XinFeng Zhang, YuanQing Zhang, Na Sun, Ying Yang, Yi Wei, Bin Hu, Xi Guo
Summary: In this study, the in-depth characterization and functional analysis of the O-antigen gene clusters in EPEC001 and EPEC080 revealed the key role of O-antigen in the pathogenesis of EPEC. Furthermore, the analysis suggested that these two EPEC strains might have acquired the O-antigen gene clusters through recombination or from uncommon ancestors.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Chemical
Tianyu Wang, Aiming Deng, Yurong He, Bin Wu, Runzhe Gao, Tianqi Tang
Summary: A two-step K-means cluster identification method is proposed in this study to capture the phenomenon of particle clustering in gas-solid interaction. The estimated solid fraction threshold is about 0.204.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Microbiology
Xi Guo, Jing Wang, Pan Wu, Shujie Li, Lu Feng, Bin Liu, Lei Wang
Summary: A molecular serotyping scheme for Morganella morganii was developed based on genetic variation, along with a traditional serotyping scheme that was shown to be consistent. Additionally, a microsphere-based suspension array with high specificity was introduced. This work provides an important tool for the detection and epidemiological surveillance of M. morganii, with potential wide applicability.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alister Burt, C. Keith Cassidy, Phillip J. Stansfeld, Irina Gutsche
Summary: This study utilized cryo-electron tomography to visualize the native-state chemosensory arrays in E. coli minicells, revealing a new p2-symmetric array architecture that differs from the previously described p6-symmetric architecture. The researchers proposed molecular models for this alternative architecture and the canonical p6-symmetric assembly, evaluating the functional implications and potential effects for future studies based on the observed data.
Article
Multidisciplinary Sciences
Paola Bonaventura, Vincent Alcazer, Virginie Mutez, Laurie Tonon, Juliette Martin, Nicolas Chuvin, Emilie Michel, Rasha E. Boulos, Yann Estornes, Jenny Valladeau-Guilemond, Alain Viari, Qing Wang, Christophe Caux, Stephane Depil
Summary: Researchers identified shared CD8(+) T cell epitopes derived from cancer-associated human endogenous retroviruses (HERVs) in solid tumors using a bioinformatic approach. These epitopes were shown to be immunogenic and capable of recognizing and killing tumor cells expressing the corresponding epitopes, highlighting their potential for the development of cancer vaccines or T cell-based immunotherapies.
Article
Chemistry, Organic
Dongbo Xu, Jackie Metz, Dedra Harmody, Tara Peterson, Priscilla Winder, Esther A. Guzman, Riccardo Russo, Peter J. McCarthy, Amy E. Wright, Guojun Wang
Summary: A novel brominated nocardiopsistin D and two new sulfur-containing nocardiopsistins were identified, showing significant anti-MRSA activities and new activities against other bacterial pathogens.
Article
Veterinary Sciences
Fimme J. van der Wal, Rene P. Achterberg, Jeanet A. van der Goot, Annemieke Dinkla, Ruth Bossers-de Vries, Conny van Solt-Smits, Alex Bossers, Marloes Heijne
Summary: The study focused on developing a differentiating serological test for antibodies against Chlamydia species in chicken sera. A bead-based Luminex suspension array was constructed using peptides as antigens, which correctly identified antibodies and differentiated between different Chlamydia species in experimentally infected mice and chickens. Although interpretation of signals in field sera was difficult due to limited samples, results were supported by published data.
VETERINARY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Rie Jonsson, Alexander Bjorling, Soren Roi Midtgaard, Grethe Vestergaard Jensen, Nicholas Skar-Gislinge, Lise Arleth, Steve Matthews, Karen Angeliki Krogfelt, Havard Jenssen
Summary: This study used small-angle X-ray scattering to reveal the structural morphology of the aggregative adherence fimbriae. The results showed that the fimbriae were arranged in a compact manner with Agg5A + Agg3B subunit proteins assembled pairwise on a flexible string. The study also suggests that the minor subunit plays a significant role in the assembled fimbriae by affecting the overall structure.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Organic
Wen-He Zhang, Fei Wang, Yi-Lin Wang, Song You, Hai-Xue Pan, Gong-Li Tang
Summary: Biochemical elucidation of the early biosynthetic pathways of miharamycins and amipurimycin revealed the important roles of several enzymes, with some hypothetical proteins unexpectedly exhibiting dual functions. In addition, the functions of carbonyl reductase MihE and rare guanine O-methyltransferase MihF were also confirmed.
Article
Food Science & Technology
Ariel Diego Roge, Ana Beatriz Celi Castillo, Claudia Andrea Ploeg, Ximena Luciana Bordagorria, Valeria M. Padin, Susana Beatriz Bruno
Summary: Two new sequences of flagellin-encoding genes in E. coli were identified in this study, each with a specific antiserum produced to confirm their expression. Sequencing of the flagellin gene offers a rapid method for determining E. coli H antigens and could be used to detect potential novel flagellar antigens.
FOODBORNE PATHOGENS AND DISEASE
(2022)
Article
Engineering, Biomedical
Mingzheng Duan, Xiaopeng Zhong, Xu Zhao, Omar M. El-Agnaf, Yi-Kuen Lee, Amine Bermak
Summary: This study introduces an inverter-based CMOS ISFET sensor array for bacterial pH detection, demonstrating the potential for rapid and cost-effective detection of multiple information of bacterial samples.
IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS
(2021)
Article
Multidisciplinary Sciences
Xiaojun Zhang, Jianbo Yuan, Yamin Sun, Shihao Li, Yi Gao, Yang Yu, Chengzhang Liu, Quanchao Wang, Xinjia Lv, Xiaoxi Zhang, Ka Yan Ma, Xiaobo Wang, Wenchao Lin, Long Wang, Xueli Zhu, Chengsong Zhang, Jiquan Zhang, Songjun Jin, Kuijie Yu, Jie Kong, Peng Xu, Jack Chen, Hongbin Zhang, Patrick Sorgeloos, Amir Sagi, Acacia Alcivar-Warren, Zhanjiang Liu, Lei Wang, Jue Ruan, Ka Hou Chu, Bin Liu, Fuhua Li, Jianhai Xiang
NATURE COMMUNICATIONS
(2019)
Correction
Microbiology
Lingyan Jiang, Lu Feng, Bin Yang, Wenwen Zhang, Peisheng Wang, Xiaohan Jiang, Lei Wang
Review
Microbiology
Bin Liu, Axel Furevi, Andrei Perepelov, Xi Guo, Hengchun Cao, Quan Wang, Peter R. Reeves, Yuriy A. Knirel, Lei Wang, Goran Widmalm
FEMS MICROBIOLOGY REVIEWS
(2020)
Article
Biochemistry & Molecular Biology
Dalong Hu, Bin Liu, Lei Wang, Peter R. Reeves
MOLECULAR BIOLOGY AND EVOLUTION
(2020)
Article
Chemistry, Applied
Xiaolong Jiang, Jing Bai, Jian Yuan, Huijing Zhang, Gege Lu, Yuhui Wang, Lingyan Jiang, Bin Liu, Lei Wang, Di Huang, Lu Feng
Summary: By reprogramming the metabolic network, the research team increased the yield of OPS-based glycoconjugate vaccines to 38.6 mg L-1, the highest reported so far. Mass spectrometry analysis showed a high glycosylation efficiency of the designed glycosylation sequon in CTB-O5 and CTB-O7, demonstrating the effectiveness of the glyco-optimized platform in producing vaccines against ExPEC.
CARBOHYDRATE POLYMERS
(2021)
Article
Immunology
Shujie Li, Yu Pang, Si Zhang, Qing Li, Qian Wang, Lei Wang, Lu Feng
Summary: This study provides the first comprehensive analysis of the transcriptome profile of UPEC during its intra-BECs life, revealing a new virulence-associated gene and its regulatory mechanism.
MICROBIAL PATHOGENESIS
(2021)
Article
Multidisciplinary Sciences
Lingyan Jiang, Peisheng Wang, Xiaorui Song, Huan Zhang, Shuangshuang Ma, Jingting Wang, Wanwu Li, Runxia Lv, Xiaoqian Liu, Shuai Ma, Jiaqi Yan, Haiyan Zhou, Di Huang, Zhihui Cheng, Chen Yang, Lu Feng, Lei Wang
Summary: S. Typhimurium infection induces upregulated glycolysis and decreased serine synthesis in host macrophages, leading to accumulation of glycolytic intermediates that promote bacterial replication and virulence.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yutao Liu, Bin Liu, Tingting Xu, Qian Wang, Wendi Li, Jialin Wu, Xiaoyu Zheng, Bin Liu, Ruiying Liu, Xingmei Liu, Xi Guo, Lu Feng, Lei Wang
Summary: The study identifies a fructose uptake system crucial for the growth of Vibrio cholerae in seawater, where fructose concentrations are low. This system may be important for the survival of pandemic V. cholerae in seawater.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Bin Liu, Chengqian Qian, Pan Wu, Xiaodan Li, Yutao Liu, Huiqian Mu, Min Huang, Yang Zhang, Tianyuan Jia, Yuanyuan Wang, Lu Wang, Xiao Zhang, Di Huang, Bin Yang, Lu Feng, Lei Wang
Summary: Enterohemorrhagic Escherichia coli regulates its cell wall structure during infection, balancing survival in the intestinal lumen and infection of intestinal epithelial cells. The bacteria express long-chain OAg in the intestinal lumen for increased resistance to antimicrobial peptides, but reduce OAg chain length upon sensing epithelial cell attachment, leading to enhanced injection of T3SS effectors into epithelial cells for host cell infection. This regulatory mechanism may be widespread in pathogenic bacteria and closely related to bacterial pathogenesis.
Article
Food Science & Technology
Min Wang, Bin Liu, Yanli Xu, Xi Guo, Tian Fu, Jiafei Liu, Lei Wang
Summary: In this study, the O-antigen gene clusters of V. fluvialis were analyzed by whole-genome sequencing, and a microsphere-based suspension array (MSA) was developed for the identification of different serogroups. Additionally, 13 new O-antigen gene cluster types were discovered. This method provides a rapid and efficient tool for detecting and identifying V. fluvialis serogroups, with potential applications in epidemiological investigations and food safety.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Cell Biology
Yu Pang, Zhihui Cheng, Si Zhang, Shujie Li, Xueping Li, Xiaodan Li, Xiao Zhang, Xiaoxiao Li, Yingxing Feng, Heting Cui, Zhen Chen, Le Liu, Qing Li, Jianxiao Huang, Mingqing Zhang, Siwei Zhu, Lei Wang, Lu Feng
Summary: Urinary tract infections are mainly caused by uropathogenic Escherichia coli (UPEC). This study reveals that UPEC senses host immune responses and initiates escape by upregulating a key phospholipase. The findings provide insights into pathogenic bacteria's response to intracellular phosphate shortage and tackling host defense, and offer potential for developing new therapeutic agents to treat UPEC infection.
Article
Cell Biology
Lu Feng, Bin Yang, Yongchang Xu, Yingying Xiong, Fang Wang, Bin Liu, Wen Yang, Ting Yao, Lei Wang
Summary: This study reveals a signal transduction pathway for the activation of virulence in EHEC O157:H7, providing insights into the relationship between adherence, gene regulation, and pathogenicity.
Article
Cell Biology
Hao Sun, Xuehua Wan, Yu Fan, Peng Liu, Yajun Song, Ningyu Zhu, Zhifeng Duan, Qian Wang, Fang Chen, Changhong Zhou, Yangyang Zheng, Peng Ding, Fenxia Liu, Lu Feng, Kwang Sik Kim, Lei Wang
Summary: The immune response of brain cells to invading bacteria and the mechanism used by pathogenic bacteria to escape immune surveillance in the brain are still not well understood. Microglia can engulf pathogenic bacteria and activate astrocytes to produce C3 for antibacterial activity. Pathogenic bacteria can sense low concentration of threonine in cerebrospinal fluid to reduce the immune response.
Article
Multidisciplinary Sciences
Bin Liu, Yutao Liu, Bin Yang, Qian Wang, Xingmei Liu, Jingliang Qin, Ke Zhao, Fan Li, Xiaohui Feng, Linxing Li, Pan Wu, Miaomiao Liu, Siwei Zhu, Lu Feng, Lei Wang
Summary: This study reveals that EHEC O157:H7 acquired a previously uncharacterized regulatory system rbfSR, which senses riboflavin produced by gut microbiota and activates the expression of virulence genes. This acquisition represents an essential step in the evolution of the highly pathogenic O157:H7. The introduction of rbfSR into other EHEC serotypes also increased their pathogenicity, indicating a potential public health issue.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)