期刊
FISH & SHELLFISH IMMUNOLOGY
卷 47, 期 2, 页码 878-885出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2015.10.031
关键词
Ayu; Cathelicidin; Chemotaxis; Monocytes/macrophages function; P2X7 receptor
资金
- Program for the National Natural Science Foundation of China [31372555]
- Zhejiang Provincial Natural Science Foundation of China [LQ13C190002, LZ13C190001]
- Research Fund for the Doctoral Program of Higher Education of China [20113305120001]
- Program for the Natural Science Foundation of Ningbo City [2014A610178]
- KC Wong Magna Fund in Ningbo University
Cathelicidins (CATHs) are a family of endogenous antimicrobial peptides that are capable of both direct bacteria-killing and immunomodulatory effects. P2X7 receptor (P2X7R) is a mediator of CATH in mammalian immune cells. Here, we studied the function and regulation of CATH in head kidney-derived monocytes/macrophages (MO/M Phi) from ayu, Plecoglossus altivelis. We investigated the chemotaxis of MO/M Phi in response to ayu CATH (PaCATH), and found that PaCATH had a dose-dependent effect on MO/M Phi chemotaxis with the optimal concentration of 10.0 mu g/ml. The qPCR and Western blot analysis revealed that PaCATH inhibited the expression of ayu P2X7R (PaP2X7R) at both mRNA and protein levels. Knockdown of the PaP2X7R expression in ayu MO/M Phi by RNA interference not only significantly inhibited the chemotactic effect of PaCATH on MO/M Phi, but also obviously reduced the effect of PaCATH on the phagocytosis, bacteria-killing, respiratory burst, and cytokine expression of ayu MO/M Phi. Our study revealed that the immunomodulatory effect of fish CATH is mediated by P2X7R. (C) 2015 Elsevier Ltd. All rights reserved.
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