期刊
FISH & SHELLFISH IMMUNOLOGY
卷 45, 期 2, 页码 912-918出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2015.06.016
关键词
Pinctada fucata; Mantle injury; Galectin; Promoter region; DNA methylation
资金
- National Science and Technology Program of China [2012AA10A410]
- Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201301A03]
- Science and Technology Program of Guangdong, China [20138020308005]
Galectin is one important member of pattern recognition proteins that plays a pivotal role in regulating innate immune response of invertebrates. In this study, we cloned the promoter sequence of a tandem-repeat galectin from the pearl oyster Pinctada fucata (P. fucata). The quantitative real-time PCR analysis revealed that galectin mRNA expression in mantle tissues were firstly up-regulated from time points of 2 h-24 h, and then down-regulated from time points of 24 h-168 h after mantle injury. The genome methylation level of mantle tissue was inversely related to galectin mRNA expression (Pearson correlation: -0.554, P: 0.154). The CpG4-6 methylation level in promoter region of galectin was significant positive correlated with the mRNA expression (Pearson correlation: 0313, P: 0.049). The results indicated that galectin gene may be involved in immune response in mantle wound healing process of P. fucata, and DNA methylation may be a regulation factor of gene expression. (C) 2015 Elsevier Ltd. All rights reserved.
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