Article
Plant Sciences
Youyue Li, Xiangkai You, Zhe Tang, Tianqi Zhu, Bowen Liu, Mo-Xian Chen, Yuefei Xu, Tie-Yuan Liu
Summary: In this study, plant growth-promoting rhizobacteria (PGPR) were isolated from the salt-treated rhizosphere of tall fescue. Bacillus zanthoxyli was found to play an important role in the growth and salt tolerance of tall fescue.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Plant Sciences
Pauline Thomelin, Julien Bonneau, Chris Brien, Radoslaw Suchecki, Ute Baumann, Priyanka Kalambettu, Peter Langridge, Penny Tricker, Delphine Fleury
Summary: Research has identified a quantitative trait locus (QTL) qYDH.3BL in wheat chromosome 3B associated with increased yield in hot and dry climates. The study found that this QTL exerts its effects on early vigour, root growth, plant biomass, and yield components, contributing to the development of heat-tolerant wheat varieties.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Agriculture, Multidisciplinary
M. C. Broberg, F. Hayes, H. Harmens, J. Uddling, G. Mills, H. Pleijel
Summary: In this study, the interactive effects of ozone, temperature, and water supply on wheat yield and grain quality were assessed. It was found that ozone, warming, and drought all decreased grain yield and average grain mass but increased grain concentration of nutrients. The results highlight the importance of considering water availability when assessing the impact of ozone on global food production.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2023)
Article
Agronomy
Nosheen Akhtar, Noshin Ilyas
Summary: Nanosilicab fertilizer has a positive effect on the growth of Triticum aestivum under drought stress, including increased germination percentage, shoot length, root length, photosynthetic pigments, osmolytes content, and antioxidant activity. It also improves hundred-grain weight and grains per spike, showing potential for application on crops.
Article
Plant Sciences
Chao Li, Kaiyong Fu, Wenting Guo, Xiangchi Zhang, Chunyan Li, Cheng Li
Summary: This study investigated the response of starch and sugar metabolism to drought stress during wheat grain filling. The grain filling rate of two elite wheat varieties under two water treatments was simulated using logistic regression analysis. The results showed that drought stress mainly affected the early period of grain filling through changes in sucrose and soluble sugar contents, sucrose synthase activity, starch content, and the activity of key starch synthases. Differential expressed proteins related to carbohydrate metabolism, particularly starch and sugar metabolism, were upregulated in response to drought stress. The study provides insights into the mechanisms of drought tolerance in wheat.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Alaa El-Dein Omara, Emad M. Hafez, Hany S. Osman, Emadeldeen Rashwan, Mohamed A. A. El-Said, Khadiga Alharbi, Diaa Abd El-Moneim, Salah M. Gowayed
Summary: This study investigated the effects of compost and plant growth-promoting rhizobacteria (PGPR) on wheat growth, productivity, and nutrient uptake under deficient irrigation and soil salinity conditions. The results showed that the combined application of compost and PGPR mitigated the negative impacts of environmental stresses, such as water stress and soil salinity, and improved growth, yield, and nutrient uptake in wheat plants.
Article
Agronomy
Larisa Fedoreyeva, Elena M. Lazareva, Olga Shelepova, Ekaterina N. Baranova, Neonila Kononenko
Summary: Soil salinity restricts plant growth, and wheat is sensitive to toxic ions. Salt-tolerant varieties accumulate more toxic ions and ROS, leading to autophagy and programmed cell death. Induction of autophagosomes and mitophagy play important roles under salt stress.
Article
Plant Sciences
Ana L. Gomez-Espejo, Carolina Paola Sansaloni, Juan Burgueno, Fernando H. Toledo, Adalberto Benavides-Mendoza, M. Humberto Reyes-Valdes
Summary: This study analyzed the genomics of adaptive traits in bread wheat landraces through genome-environment associations. The results identified several genes and proteins involved in the response and tolerance to abiotic stress, with some genes mediated by auxins, abscisic acid, ethylene, salicylic acid, and jasmonates. Additionally, proteins associated with heat stress, drought, and cell wall functions were identified.
Article
Biochemistry & Molecular Biology
Weiwei Wang, Jinliang Guo, Jiayang Ma, Zhulin Wang, Lining Zhang, Zixu Wang, Min Meng, Chao Zhang, Fengli Sun, Yajun Xi
Summary: The transformation efficiency of wheat embryos was improved using a combination of special chemical and physical methods with the PureWheat technique. Scanning electron microscopy (SEM), transcriptome analysis and metabolome analysis were conducted to understand the reaction of immature embryos infected by Agrobacterium tumefaciens in PureWheat. The SEM analysis revealed that Agrobacterium tumefaciens was deposited under the damaged cortex of embryos and contacted the receptor cells to improve transformation efficiency. Transcriptome analysis showed that differentially expressed genes were involved in various metabolic pathways and signaling pathways, indicating the energy-demanding process of Agrobacterium infection. This study provides insights into the physiological and molecular changes of wheat embryos during the co-culture stage and sheds light on Agrobacterium-mediated transgenic experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Neonila Kononenko, Elena Lazareva, Larisa Fedoreyeva
Summary: Various stressors lead to an increase in ROS and damage to plant tissues. Different wheat varieties show different resistances to abiotic stresses due to variations in the activation of antioxidant system components and glutathione content.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Milan Mirosavljevic, Sanja Mikic, Ankica Kondic Spika, Vesna Zupunski, Rong Zhou, Lamis Abdelhakim, Carl-Otto Ottosen
Summary: High temperature can reduce winter wheat grain yield by impacting grain number and weight. The study explores the influence of high temperatures on spike grain distribution and weight at different growth stages, and identifies genotypic variation among studied wheat cultivars. Results show that heat stress decreases spike grain weight, thousand-grain weight, and grain number per spike moderately with individual heat stress, and severely with multiple heat stress at anthesis and mid-grain filling stages. Heat stress also reduces the number of spikelets with grains, while grain weight at specific positions within the spike shows a positive relationship with spike grain weight. Serbian wheat cultivars Suboticanka and Renesansa are recognized as the most heat tolerant and sensitive, respectively.
PLANT SOIL AND ENVIRONMENT
(2021)
Article
Plant Sciences
Stefan M. Markovic, Dragan Zivancev, Daniela Horvat, Aleksandra Torbica, Jovana Jovankic, Nevena H. Djukic
Summary: Heat stress is a significant factor affecting wheat production, with eEF1A playing a role in plant heat tolerance. Varieties with higher eEF1A accumulation under heat stress show less decrease in photosynthetic pigments, correlating with better yield traits. This study aims to develop agricultural strategies and heat-tolerant wheat varieties through analyzing molecular mechanisms under high and moderate air temperatures.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Liuping Chen, Qian Lv, Weibing Yang, Hui Yang, Qiaoyan Chen, Bingxin Wang, Yanhong Lei, Yanzhou Xie
Summary: Drought stress has a significant impact on crop yield and quality. The study reveals the important role of TaNBR1 gene in response to drought stress. Overexpression of TaNBR1 reduces drought tolerance and affects the expression of stress-related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Toni Mohr, James Horstman, Yong Q. Gu, Nagwa I. Elarabi, Naglaa A. Abdallah, Roger Thilmony
Summary: The highly conserved Sal1 gene has been edited in hexaploid wheat using precise gene editing techniques. The resulting mutant wheat plants with disabled Sal1 genes exhibit slower growth under drought conditions and slower senescence under water limiting conditions. The use of multiplexed gRNAs enabled effective targeted gene editing of the Sal1 gene family.
Article
Plant Sciences
Mengesha T. Dugasa, Xue Feng, Nian-Hong Wang, Junmei Wang, Feibo Wu
Summary: The study compared the genome-wide transcriptome of two wheat cultivars in response to drought and salinity stresses, identifying specific genes associated with tolerance in the drought/salt-tolerant cultivar. Functional enrichment analysis revealed differences in gene expression related to ROS detoxification, defense, phytohormones, and signal transduction pathways between the two cultivars under stress conditions. These findings suggest potential molecular bases for breeding drought and salinity tolerant wheat cultivars.
PLANT GROWTH REGULATION
(2021)
Article
Plant Sciences
Madhu, Amandeep Kaur, Shivi Tyagi, Shumayla, Kashmir Singh, Santosh Kumar Upadhyay
Summary: A total of seven GR genes were identified in wheat, revealing their important role in abiotic stress response. Structural characterization and expression profiling provided insights into the characteristics and mechanisms of GR genes through phylogenetic analysis.
PLANT CELL REPORTS
(2022)
Article
Plant Sciences
Deepika Verma, Santosh Kumar Upadhyay, Kashmir Singh
Summary: APX and APX-R gene families were identified and characterized in two important oilseed species of Brassica, showing responses to drought and heat stress. Two stress-responsive genes were cloned and characterized, demonstrating their roles under various stress conditions in a bacterial expression system. This study sheds light on the potential development of abiotic stress-tolerant Brassica crops.
PLANT CELL REPORTS
(2022)
Article
Plant Sciences
Madhu, Alok Sharma, Amandeep Kaur, Shivi Tyagi, Santosh Kumar Upadhyay
Summary: This review discusses the importance of glutathione peroxidases (GPXs) in plant physiology, including their role in scavenging reactive oxygen species, maintaining redox homeostasis, and responding to environmental stresses. The study of GPX genes provides valuable information for genetic engineering to enhance agricultural plant yields under unfavorable conditions.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Alok Sharma, Himanshu Sharma, Ruchika Rajput, Ashutosh Pandey, Santosh Kumar Upadhyay
Summary: A series of stress-responsive TLP genes were identified in the genomes of wheat and other cereal crops, and their roles in plant development and stress response were revealed through evolutionary and expression analyses, providing important references for future crop improvement programs.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Himanshu Sharma, Alok Sharma, Ruchika Rajput, Sukhjeet Sidhu, Harpal Dhillon, Praveen Chandra Verma, Ashutosh Pandey, Santosh Kumar Upadhyay
Summary: In this study, 29 BOR genes were identified and analyzed for their conserved nature. The role of duplication events and purifying selection in the expansion of the BOR family in wheat was revealed. The study also showed the expression and co-expression patterns of BOR genes under biotic and abiotic stress conditions, as well as their involvement in B stress management.
Article
Plant Sciences
Meenakshi Rathour, Shumayla, Anshu Alok, Santosh Kumar Upadhyay
Summary: The present study provides a comprehensive investigation of TaTALE genes in bread wheat, revealing their important roles in various biological functions, particularly in development and stress response. The study also provides insights into the gene and protein structural properties, phylogeny, expression patterns, and multilevel gene regulations of TaTALE genes. These findings contribute to further functional validation of these genes in future studies.
Article
Plant Sciences
Amandeep Kaur, Alok Sharma, Madhu, Praveen Chandra Verma, Santosh Kumar Upadhyay
Summary: In this study, 586 EF-hand domain-containing proteins were identified in Triticum aestivum, and their involvement in plant growth, development, and stress responses was revealed. The interaction of these proteins with miRNA and Ca2+ binding/sensing proteins was analyzed, providing valuable information for the functional characterization of potential candidate genes in future studies.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Amandeep Kaur, Alok Sharma, Sameer Dixit, Kashmir Singh, Santosh Kumar Upadhyay
Summary: Through in silico approaches, this study identified 42 OSCA genes in the T. aestivum genome and revealed their evolutionary history and potential functional mechanisms. The experimental results suggest the involvement of OSCA genes in wheat development and adaptive responses, potentially through Ca2+-mediated biological processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Santosh Kumar Upadhyay
Summary: Stomatal immunity is regulated by PAMPs and ABA-induced signaling, with cytoplasmic Ca2+ playing a crucial role. The influx of Ca2+ is mediated by OSCAs and CNGCs, which are activated by BIK1-mediated phosphorylation and ABA interaction. Additionally, ABA triggers OST1 activation, leading to microtubule disassembly and SLAC1 activation for cytoplasmic anion efflux and stomatal closure.
Review
Agronomy
Sanchita Singh, Somnath Rahangdale, Shivali Pandita, Gauri Saxena, Santosh Kumar Upadhyay, Geetanjali Mishra, Praveen C. Verma
Summary: CRISPR/Cas9 technology has shown great potential in controlling insect pests by editing their genomes, leading to more effective pest management strategies. Researchers have highlighted the efficiency and future possibilities of using CRISPR/Cas9 in pest control.
Review
Agronomy
Ahamed Khan, Noohi Nasim, Baveesh Pudhuvai, Bhupendra Koul, Santosh Kumar Upadhyay, Lini Sethi, Nrisingha Dey
Summary: This review discusses the application of synthetic promoters in plants to overcome the limitations of native promoters in terms of size, distribution, and induction, which ultimately leads to insufficient transcriptional activity. It emphasizes the basic structure and function of the promoter, with a focus on the role of Cis-elements in gene expression regulation. The importance of synthetic promoters in the field of plant biology is highlighted, along with information on two major approaches: conventional methods and advancements in gene editing technology. The efficiency of synthetic promoters and the expression level of transgenes are crucial for successful plant genetic manipulation, making the development of synthetic promoters highly promising for genetic engineering-mediated crop improvement.
Article
Agronomy
Amandeep Kaur, Alok Madhu, Alok Sharma, Kashmir Singh, Santosh Kumar Upadhyay
Summary: This study identified three Piezo genes in the Triticum aestivum genome and found that they are involved in plant growth and development and response to various stresses, potentially related to calcium ion signaling.
Article
Environmental Sciences
Shivi Tyagi, Yashraaj Sharma, Alok Sharma, Ashutosh Pandey, Kashmir Singh, Santosh Kumar Upadhyay
Summary: Cadmium is a toxic heavy metal naturally occurring in the environment. Exogenous application of calcium has been found to reduce the toxicity of cadmium in crop plants. The TaNCL2-A gene has been shown to play a role in the response to cadmium stress and its overexpression leads to increased cadmium tolerance and calcium accumulation in transgenic Arabidopsis lines. In addition, these transgenic lines also exhibit improved growth, yield parameters, and physiological indicators compared to control plants, and show tolerance to salinity and osmotic stress.
Editorial Material
Biochemistry & Molecular Biology
Santosh Kumar Upadhyay
Summary: A deficiency in inorganic phosphate (Pi) triggers the expression of miRNA399 and the accumulation of PHT1s mRNA, which goes against the goal of miRNA-mediated gene regulation. A recent study has found a new mechanism involving the formation of RNA/RNA-duplex between a Pi deficiency-induced long non-coding RNA (PILNCR2) and PHT1s transcripts, which prevents the binding and cleavage of miRNA399 to PHT1 mRNAs, providing tolerance to Pi-deficient conditions. The translation process of PHT1 transporter proteins through the RNA/RNA-duplex remains unknown.
Article
Biochemistry & Molecular Biology
Yashraaj Sharma, Alok Sharma, Kashmir Madhu, Santosh Kumar Shumayla, Kashmir Singh, Santosh Kumar Upadhyay
Summary: Long non-coding RNAs (lncRNAs) are transcripts without protein-coding potential that play important roles in plant survival by regulating gene expression through epigenetic changes, transcription, and post-transcription. In particular, lncRNAs have diverse mechanisms in plant growth, development, and stress responses, with a focus on their potential against biotic stress. Further research is needed to better understand the functions and molecular mechanisms of lncRNAs in plant responses to pathogens.