Article
Genetics & Heredity
Wei Ge, Weidong Zhang, Yuelang Zhang, Yujie Zheng, Fang Li, Shanhe Wang, Jinwang Liu, Shaojing Tan, Zihui Yan, Lu Wang, Wei Shen, Lei Qu, Xin Wang
Summary: This study conducted single-cell RNA sequencing on 19,705 single cells from the dorsal skin of cashmere goat fetuses at different developmental stages, revealing cell differentiation and types, as well as molecular landscape along dermal and epidermal cell lineage pathways. The heterogeneity of dermal papillae from different hair follicle types was confirmed and validated, providing insight into biomarkers for cashmere goat hair follicle development and potential implications for breeding programs.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2021)
Article
Cell Biology
Tao Ma, Jianyu Li, Jianping Li, Sufang Wu, Xiangba, Huaizhi Jiang, Qialling Zhang
Summary: Research has shown that miRNA-203 regulates the development of hair follicles in Cashmere goats by targeting the genes DDOST and NAE1, potentially leading to increased domestic cashmere yield.
Article
Agriculture, Dairy & Animal Science
Chunhui Duan, Lechao Zhang, Kun Gao, Yunxia Guo, Yueqin Liu, Yingjie Zhang
Summary: This study investigated the differences in cashmere traits, yield, hair follicle characteristics, and fiber-style-related genes between Yanshan cashmere goats with non-crimped and crimped fleece. The results revealed that goats with crimped fleece produced a higher yield of cashmere with finer diameter fibers compared to goats with non-crimped fleece. Mutations in the KRT1 and KRT6A genes may be associated with these differences in fiber properties.
Article
Agriculture, Dairy & Animal Science
C. H. Yang, C. H. Duan, Z. Y. Wu, Y. Li, Y. Y. Luan, X. J. Fu, C. X. Zhang, W. Zhang
Summary: The melatonin treatment has short-term effects on cashmere production and hair follicle populations in Inner Mongolian Cashmere goats, but no residual effects on consecutive cashmere growth cycles.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Yuan Gao, Lei Duo, Xiaoshu Zhe, Lingyun Hao, Weiguo Song, Lizhong Gao, Jun Cai, Dongjun Liu
Summary: This study investigated the mechanism of embryonic hair follicle development in cashmere goats using proteomic and metabolomic techniques. The results showed that the oxytocin signalling pathway plays a crucial role in embryonic hair follicle development by activating the MAPK and Ca2+ signalling pathways.
Article
Agriculture, Dairy & Animal Science
Xiaogao Diao, Lingyun Yao, Xinhui Wang, Sen Li, Jiaxin Qin, Lu Yang, Liwen He, Wei Zhang
Summary: The development of secondary hair follicles in goat kids is complete at 5-6 months of age. FGF2, FGF21, and BMP7 may promote the growth of secondary hair follicles, and the optimal period for their regulation is from birth to 5-6 months of age. Hair follicle traits at 6 months of age can be used as an index for breeding selection of cashmere goats.
Article
Multidisciplinary Sciences
Taiyu Hui, Yuanyuan Zheng, Chang Yue, Yanru Wang, Zhixian Bai, Jiaming Sun, Weidong Cai, Xinjiang Zhang, Wenlin Bai, Zeying Wang
Summary: Competitive endogenous RNA (ceRNA) is a type of transcript that can be mutually regulated by shared miRNAs. This study identified miRNAs in Liaoning Cashmere Goat (LCG) skin and analyzed the expression of mRNAs, lncRNAs, and circRNAs in both LCG and Inner Mongolia cashmere goat (MCG) skin. Through further analysis and confirmation, a ceRNA regulatory network for controlling cashmere fineness was established.
SCIENTIFIC REPORTS
(2021)
Article
Veterinary Sciences
Wei Yan, Fei Hao, Xiaoshu Zhe, Yingmin Wang, Dongjun Liu
Summary: This study confirmed the biological characteristics and differentiation potential of PHFSCs and SHFSCs from Arbas Cashmere goats, expanding and refining our knowledge of adult stem cell types and characteristics.
BMC VETERINARY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Guishan Zhang, Jing Xu, Yingnan Zhang, Shubao Yang, Huaizhi Jiang
Summary: In this study, it was found that miRNA-1-3p may regulate hair follicle development in Liaoning Cashmere goats by targeting FGF14. This finding contributes to a better understanding of the molecular mechanisms underlying goat hair follicle development.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Wen Zheng Gao, Hui Ling Xue, Jian Cheng Yang
Summary: The study reveals that keratin proteins are expressed differently in various stages of the goat hair follicle cycle, and nine specific keratin proteins can serve as markers for the cycle. These keratin proteins are believed to have common evolutionary precursors, and functional enrichment analysis indicates the importance of estrogen and ECM-receptor interaction signaling pathways in the hair follicle cycle.
SMALL RUMINANT RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Yanyu He, Xiu Liu, Jie De, Saihong Kang, John S. Munday
Summary: Cashmere goats in Tibet are adapted to high altitude, cold climates, and hypoxia. This study compared the morphology and immunostaining of hair follicle regulatory proteins in Tibetan cashmere goats from high and low altitude regions. Results showed that altitude and temperature influence the development of secondary hair follicles, with significant differences observed between goats from different locations. The understanding of these factors can help maximize the production of valuable fibers.
Article
Veterinary Sciences
Zhihong Liu, Zhichen Liu, Qing Mu, Meng Zhao, Ting Cai, Yuchun Xie, Cun Zhao, Qing Qin, Chongyan Zhang, Xiaolong Xu, Mingxi Lan, Yanjun Zhang, Rui Su, Zhiying Wang, Ruijun Wang, Zhixin Wang, Jinquan Li, Yanhong Zhao
Summary: This study investigates the mechanism by which melatonin regulates cashmere growth through the implantation of melatonin. Through gene co-expression network analysis and differential gene expression analysis, key genes and pathways related to the cashmere growth cycle after melatonin implantation were identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yuelang Zhang, Fang Li, Yujie Shi, Tongtong Zhang, Xin Wang
Summary: This study identified differentially expressed lncRNAs and miRNAs during cashmere morphogenesis in cashmere goat skin using high-throughput sequencing and bioinformatics analysis. The results showed that ECM-receptor interaction and amino acid biosynthesis were crucial for hair follicle development, and Notch, TGF-beta, and Wnt signaling pathways played important roles. A ceRNA regulatory network was also constructed, and the lncRNA H19 was found to promote the viability and proliferation of dermal papilla cells through the chi-miR-214-3p/beta-catenin axis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Minglin Wang, Han Dai, Shengda Sheng, Yanlei Liu, Shuyi Zhang, Wenlin Bai, Huiling Xue
Summary: This study investigated the role of miRNAs in cashmere growth by analyzing the expression of miRNAs in SHFs. The results showed that the early anagen phase is crucial for miRNA regulation of cashmere growth. Furthermore, differential expression analysis identified several miRNAs that are involved in SHF development, regeneration, and regression. The study also discovered a potential novel role of miR-143-3p in cashmere growth through its direct repression of Itga6 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
Rong Ma, Fangzheng Shang, Youjun Rong, Jianfeng Pan, Min Wang, Shuran Niu, Yunpeng Qi, Yanbo Li, Qi Lv, Zhiying Wang, Ruijun Wang, Rui Su, Zhihong Liu, Yanhong Zhao, Zhixin Wang, Jinquan Li, Yanjun Zhang
Summary: This study used high-throughput sequencing to explore the role of long non-coding RNAs (lncRNAs) in hair follicle development in Inner Mongolian cashmere goats. The results showed that lncRNAs may interact with miRNAs and mRNAs to regulate the expression of genes related to hair follicle development. The study provides insights into the molecular regulation mechanism of hair follicle development and cashmere hair follicle growth.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Jingwei Yuan, Shijun Li, Zheya Sheng, Meikun Zhang, Xuming Liu, Zhengdong Yuan, Ning Yang, Jilan Chen
Summary: High genetic diversity is observed in Tibetan native chickens, with intense inbreeding in the Nyingchi population. Candidate genes in ROH islands are likely to be the drivers of adaptation to high altitude exhibited by the Tibetan native chicken populations.
Article
Biotechnology & Applied Microbiology
Weiwei Fu, Rui Wang, Naiyi Xu, Jinxin Wang, Ran Li, Hojjat Asadollahpour Nanaei, Qinghua Nie, Xin Zhao, Jianlin Han, Ning Yang, Yu Jiang
Summary: Galbase is an easily accessible repository that integrates public chicken multi-omics data, providing new opportunities for in-depth functional genomic studies on chicken and identification of genetic variations responsible for chicken complex traits.
Article
Biochemistry & Molecular Biology
Ming Li, Congjiao Sun, Naiyi Xu, Peipei Bian, Xiaomeng Tian, Xihong Wang, Yuzhe Wang, Xinzheng Jia, Rasmus Heller, Mingshan Wang, Fei Wang, Xuelei Dai, Rongsong Luo, Yingwei Guo, Xiangnan Wang, Peng Yang, Dexiang Hu, Zhenyu Liu, Weiwei Fu, Shunjin Zhang, Xiaochang Li, Chaoliang Wen, Fangren Lan, Amam Zonaed Siddiki, Chatmongkon Suwannapoom, Xin Zhao, Qinghua Nie, Xiaoxiang Hu, Yu Jiang, Ning Yang
Summary: The gene numbers and evolutionary rates of birds are lower than those of mammals, but birds have a large species number and morphological diversity. To understand avian evolution, it is necessary to study the complete avian genome. A chicken pan-genome was constructed from 20 de novo assembled genomes, revealing novel protein-coding genes and long noncoding RNAs not found in previous databases. These hidden genes were found to be shared by all chicken genomes, including many housekeeping genes, and were enriched in immune pathways. Comparative genomics showed that these novel genes have higher substitution rates than known genes, updating our knowledge about evolutionary rates in birds. This study provides a framework for constructing a better chicken genome, contributing to the understanding of avian evolution and improvement of poultry breeding.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Agriculture, Dairy & Animal Science
Jianbo Li, Xiuan Zhang, Xiqiong Wang, Congjiao Sun, Jiangxia Zheng, Junying Li, Guoqiang Yi, Ning Yang
Summary: This study established a comprehensive m6A methylation profile of chicken embryonic gonads using MeRIP-seq and identified YTHDC2 as a key regulator in sex differentiation. The results demonstrate that m6A methylation plays an important role in chicken gonad differentiation, providing a better understanding of the epigenetic factors involved in chicken sex determination and differentiation.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Jianbo Li, Congjiao Sun, Jiangxia Zheng, Junying Li, Guoqiang Yi, Ning Yang
Summary: This study provides a comprehensive profile of four representative stages of chicken gonadal development at the transcriptional and chromatin levels. The findings suggest that candidate genes involved in morphogenesis, meiosis, and subcellular structure formation may contribute to asymmetric gonadal development. Further analysis reveals that chromatin accessibility and transcriptional activity of these candidate genes may be regulated by nearby open chromatin regions, acting as potential cis-regulatory elements.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Qianqian Zhou, Fangren Lan, Shuang Gu, Guangqi Li, Guiqin Wu, Yiyuan Yan, Xiaochang Li, Jiaming Jin, Chaoliang Wen, Congjiao Sun, Ning Yang
Summary: In this study, the researchers investigated the effects of host genetics and gut microbiota on feed efficiency in laying hens. They found that genetic factors and gut microbiota both play important roles in determining feed efficiency, with RFI having the highest microbial influence. They also identified three microbial genera that were negatively correlated with RFI and positively contributed to improving feed efficiency.
Article
Genetics & Heredity
Xiuan Zhang, Jianbo Li, Xiqiong Wang, Yuchen Jie, Congjiao Sun, Jiangxia Zheng, Junying Li, Ning Yang, Sirui Chen
Summary: In this study, a sex-reversed chicken flock was created by injecting an aromatase inhibitor, fadrozole, and high-resolution profiles of gene expression and chromatin accessibility in embryonic gonads were constructed. The results showed that fadrozole affected the transcriptional activities of genes related to sex determination and differentiation and regulated the expression of genes related to gonadal development through nearby open chromatin regions. After sexual maturity, the sex-reversed chickens were found to be infertile, and promising genes related to spermatogenesis and infertility were identified through RNA-seq. This study provides important insights into the molecular basis of sex differentiation and infertility in sex-reversed chickens and lays a foundation for further research on bird sex development.
EPIGENETICS & CHROMATIN
(2023)
Article
Biology
Junxiao Ren, Zhengzheng Hu, Quanlin Li, Shuang Gu, Fangren Lan, Xiqiong Wang, Jianbo Li, Junying Li, Liwa Shao, Ning Yang, Congjiao Sun
Summary: Chicken embryonic diapause is a state of temporary arrest of embryonic development triggered by cold stress-induced IRF1 expression, mediated by the PKC-NF-kappa B signaling pathway. This is in contrast to mammalian diapause which is mTOR-dependent. The study characterized the dynamic transcriptomic and phosphoproteomic profiles of chicken embryos in different diapause states and identified key regulators of diapause.
Review
Biochemistry & Molecular Biology
Xiuan Zhang, Jianbo Li, Sirui Chen, Ning Yang, Jiangxia Zheng
Summary: Sex determination and differentiation in birds involves genetic factors controlling the sex determination program, while sex differentiation is influenced by hormones and can be reversed when disturbed. The regulatory mechanism of avian cell autonomous sex identity and sex development is still not clearly understood and requires further exploration. However, avian sex research findings have provided theoretical bases for gender control technologies in poultry production, which are being improved through interdisciplinary cooperation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Chaoliang Wen, Qinli Gou, Shuang Gu, Qiang Huang, Congjiao Sun, Jiangxia Zheng, Ning Yang
Summary: This study investigated the composition of microbial communities in chicken muscle tissue and their relationship with meat quality. The results showed that the microbial composition in muscle tissue can affect the fat content, and there is also a correlation between fat content in different muscle tissues. These findings provide important insights for improving meat quality in chickens.
Article
Agriculture, Dairy & Animal Science
Yiming Yuan, Gang Zheng, Zhen You, Lulu Wang, Ziyi Wang, Congjiao Sun, Changjun Liu, Xianyao Li, Peng Zhao, Yongqiang Wang, Ning Yang, Ling Lian
Summary: This study used whole genome bisulfite sequencing (WGBS) and RNA-seq to identify genes playing important roles in the tumor transformation of Marek's disease (MD). 8 genes, including CD4 and HMGB1, were found to be negatively correlated with methylation and showed high expression in the MDV-infected group. Overexpression of CD4 and HMGB1 significantly promoted MDCC-MSB-1 cell proliferation, suggesting their potential promoting roles in MD tumorigenesis.
Article
Multidisciplinary Sciences
Zhangyuan Pan, Ying Wang, Mingshan Wang, Yuzhe Wang, Xiaoning Zhu, Shenwen Gu, Conghao Zhong, Liqi An, Mingzhu Shan, Joana Damas, Michelle M. Halstead, Dailu Guan, Nares Trakooljul, Klaus Wimmers, Ye Bi, Shang Wu, Mary E. Delany, Xuechen Bai, Hans H. Cheng, Congjiao Sun, Ning Yang, Xiaoxiang Hu, Harris A. Lewin, Lingzhao Fang, Huaijun Zhou
Summary: This study identified and characterized regulatory elements in the chicken genome by integrating genome-wide sequencing datasets from 23 adult tissues. It annotated 1.57 million regulatory elements, predicted enhancer-gene pairs, and super-enhancers. These findings have significant implications for understanding gene regulation in chicken domestication, selection, and complex trait regulation.
Article
Biotechnology & Applied Microbiology
Xinwei Jiang, Boxuan Zhang, Fangren Lan, Conghao Zhong, Jiaming Jin, Xiaochang Li, Qianqian Zhou, Junying Li, Ning Yang, Chaoliang Wen, Congjiao Sun
Summary: Blood biochemical indicators are regulated by both host genetics and gut microbiota, and their interrelationships can affect growth traits in chickens. The heritability and microbiability of these indicators vary, and several candidate genes and microbial species have been identified as potentially involved in their regulation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Ziyi Wang, Yiming Yuan, Gang Zheng, Meng Sun, Qinyuan Wang, Junfeng Wu, Junying Li, Congjiao Sun, Yongqiang Wang, Ning Yang, Ling Lian
Summary: This study reveals the distribution of ALVE in 11 chicken breeds, including the identification of 23 novel ALVEs in Chinese domestic breeds. Most of these insertion sites are located in intergenic regions and introns.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Chaoliang Wen, Chunning Mai, Ronglang Cai, Qinli Gou, Boxuan Zhang, Junying Li, Congjiao Sun, Ning Yang
Summary: Duration of fertility (DF) is an important economic trait in poultry production, and its correlation with egg production and breeding efficiency has been analyzed. The heritability of DF traits is low, but improvement of DF is possible through selection. Fertility duration day (FDD) is an effective selection criterion for improving DF, and it can be incorporated into a breeding program to enhance reproductive efficiency.
GENETICS SELECTION EVOLUTION
(2022)