Article
Chemistry, Medicinal
Fernanda Malanconi Thomaz, Jussara de Jesus Simao, Viviane Simoes da Silva, Meira Maria Forcelini Machado, Lila Missae Oyama, Eliane Beraldi Ribeiro, Maria Isabel Cardoso Alonso Vale, Monica Marques Telles
Summary: The study found that Ginkgo biloba extract (GbE) can modulate adipocyte differentiation, increasing lipid content and gene expression of major adipogenesis markers. GbE can modify key proteins involved in the differentiation process and accelerate the expression of mature adipocyte proteins.
Article
Agricultural Engineering
Qihui Dong, Jun Cao, Luyao Chen, Jiarui Cao, Huimin Wang, Fuliang Cao, Erzheng Su
Summary: Botanical natural bioactive ingredients are difficult to purify due to their complicated composition and structure, and are usually utilized in the form of phytocomplex, which refers to a mixture of multiple bioactive ingredients. Using biocompatible solvents as delivery solvents is a simple, versatile, and scalable method to improve the solubility of phytocomplex. In this work, the extract of Ginkgo biloba leaves (EGb) was chosen as the model of the phytocomplex to systematically investigate the potential of natural deep eutectic solvents (NADESs) as solubilizers.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Nutrition & Dietetics
Tingting Chen, Yiqiang Chen, Kaiyuan Li, Zhuo Chen, Qingyu Zhao, Yimeng Fan, Ying Liu, Suxia Zhang, Zhihui Hao
Summary: Ginkgo biloba extract (GBE) can modulate intestinal microbiota, decrease pro-inflammatory factors, elevate intestinal integrity, and protect animals from Citrobacter rodentium (CR)-induced colitis. Therefore, GBE should be seriously considered in the preventive control of CR-induced colitis and the development of effective and safe therapeutic strategies for controlling IBD.
Article
Chemistry, Medicinal
Yun Wang, Yuanyuan Xu, Xiaowei Xu, Hong Wang, Dong Wang, Wenchao Yan, Jiaying Zhu, Haiping Hao, Guangji Wang, Lijuan Cao, Jun Zhang
Summary: This study confirmed the beneficial effects of Ginkgo biloba leave extract (GbE) in alleviating hypercholesterolemia, inflammation, and atherosclerosis. GbE treatment reshaped the intestinal microbiota, downregulated proinflammatory cytokines, and enhanced the expression of tight junction proteins. It also reversed the dysfunction of microbial metabolic phenotypes and reduced atherosclerotic plaque areas. These findings provide important insights into the pharmacological mechanism underlying the antiatherogenic efficacy of GbE.
PHYTOTHERAPY RESEARCH
(2022)
Article
Plant Sciences
Han-Yang Lin, Wen-Hao Li, Chen-Feng Lin, Hao-Ran Wu, Yun-Peng Zhao
Summary: This article provides detailed information on the biology of Ginkgo biloba, covering its ecological characteristics, distribution, habitat, responses to environment and biotic factors, physiological structure, reproductive and seed characters, and the impact of diseases and pests. Ginkgo has a strong adaptability globally, with high tolerance to temperature variations, drought, and fire. Stem rot and leaf blight are common diseases in nursery seedlings and juvenile trees, which can be mitigated through shading and fertilization. Ginkgo biloba is one of the world's most distinctive trees, with significant importance in plant evolution and human society.
JOURNAL OF ECOLOGY
(2022)
Article
Chemistry, Physical
Shujun Chen, Hai Zhang, Yujie Qiang, Bochuan Tan, Yuanhui Wu, Siyi Chen
Summary: A self-assembled monolayer (SAM) based on Ginkgo biloba exocarp extract (GBEE) was successfully formed on a copper surface, exhibiting efficient inhibition behavior. Characterization of the GBEE SAM using Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and contact angle tests confirmed the hydrophobic nature of the SAM. Electrochemical measurements and scanning electron microscopy revealed that the SAM showed excellent anti-corrosion performance with a high inhibition efficiency of 97.86%. Theoretical calculations were also conducted to further understand the adsorption and inhibition mechanism of the GBEE SAM.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Medicinal
Jia Ke, Meng-Ting Li, Ya-Jing Huo, Yan-Qiong Cheng, Shu-Fen Guo, Yang Wu, Lei Zhang, Jianpeng Ma, Ai-Jun Liu, Yan Han
Summary: Our study showed that the combination of GBE50 and aspirin could enhance the antiplatelet effects by synergistic and additive effects, with stronger inhibition of platelet aggregation. GBE50 interfered with platelet aggregation through cAMP pathway, AA metabolism and calcium signaling pathway, while aspirin regulated platelet aggregation through AA metabolism and platelet activation. Demonstrating the potential application of GBE50 as a supplementary therapy for thrombosis-related diseases.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Ke Wang, Yuan Chen, Xue Zhu, Wenjun Zou, Fanfan Zhou
Summary: The study demonstrates the protective effect of Ginkgo biloba extract (GBE) on photoreceptors against white light-induced oxidative damage. This effect is mediated through the modulation of CAV-1-redoxosome signaling, showing potential therapeutic benefits in preventing retinal degenerative diseases.
Letter
Chemistry, Multidisciplinary
Zarko Kulic, Juergen Wolff, Elke Wilhelm, Vincent Schueler, Birgit Roeck, Andreas Butterer
Summary: Shikimic acid is a valuable raw material used for the synthesis of the antiviral drug oseltamivir. This study proposes a novel sustainable source for shikimic acid by utilizing waste streams from the production of Ginkgo biloba leaf extract, EGb 761.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Review
Medicine, General & Internal
Magdalena Sereda, Jun Xia, Polly Scutt, Malcolm P. Hilton, Amr El Refaie, Derek J. Hoare
Summary: This systematic review evaluated the effects of Ginkgo biloba for tinnitus in adults and children. The findings suggest that Ginkgo biloba may have little to no effect on reducing tinnitus symptoms compared to placebo. The evidence for other secondary outcomes was uncertain. The effectiveness and safety of Ginkgo biloba with concurrent interventions also remain unclear. Future research should use rigorous methodology and validated outcome measures.
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2022)
Article
Plant Sciences
Marija Kovac Tomas, Iva Jurcevic, Dunja Samec
Summary: In this study, a HPLC-DAD method was developed to separate and determine five major biflavonoids in ginkgo. Tissue-specific profiling revealed that biflavonoids were not detected in plant parts not in direct contact with the environment. Leaves had the highest total biflavonoids content, with sciadopitysin being the predominant compound, while amentoflavone was the predominant biflavonoid in the bark.
Article
Biochemistry & Molecular Biology
Shuang Su, Xue-Qiong Yang, Ya-Bin Yang, Zhong-Tao Ding
Summary: Five new terpenoids, including a polyketide-terpenoid hybrid paraphaone and four eremophilane sesquiterpenoids, were isolated from the endophytic fungus Paraphaeosphaeria sp. cultured with Ginkgo biloba extract. These compounds exhibited significant antifungal and antifeedant activities against various phytopathogens and entomopathogens. The culture of Paraphaeosphaeria sp. using Ginkgo biloba medium could be a potential source of agricultural antibiotics.
Review
Pharmacology & Pharmacy
Zarko Kulic, Martin D. Lehner, Gunnar P. H. Dietz
Summary: The phytochemical composition of extracts derived from the same plant can vary significantly, affecting their pharmacokinetic and pharmacodynamic properties, and ultimately their clinical efficacy and safety. Regulating active constituent levels and reducing undesired substances is vital for ensuring the quality and consistency of herbal extracts. The heterogeneity of extracts should be carefully considered when evaluating the efficacy and safety of plant-derived remedies.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Clinical Neurology
Lars Decker, Dietmar Basta, Martin Burkart, Arne Ernst
Summary: The study demonstrated that the combination of VNF added to EGb 761 showed significant efficacy in improving age-related vertigo and dizziness, achieving clinically relevant improvement within 12 weeks.
FRONTIERS IN NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Qingqing Wang, Zihou Liu, Rui Wang, Run Li, Xiaoru Lian, Yanquan Yang, Jiao Yan, Zhiqi Yin, Guangji Wang, Jianguo Sun, Ying Peng
Summary: This study investigated the additional benefits of Ginkgo biloba extract (GBE) in combination with statins for the treatment of dyslipidaemia. The results showed that the combination therapy significantly improved blood lipid parameters, reduced liver fat content, and had additional liver protection. GBE also regulated abnormal lipid metabolism and increased the exposure of statins in plasma, providing important implications for disease progression and statin resistance.
Article
Geriatrics & Gerontology
Robertina Giacconi, Blanca Laffon, Solange Costa, Armanda Teixeira-Gomes, Fabrizio Maggi, Lisa Macera, Pietro Giorgio Spezia, Francesco Piacenza, Alexander Buerkle, Maria Moreno-Villanueva, Stefano Bonassi, Vanessa Valdiglesias, Joao Paulo Teixeira, Martijn E. T. Dolle, M. Liset Rietman, Eugene Jansen, Tilman Grune, Efstathios S. Gonos, Claudio Franceschi, Miriam Capri, Birgit Weinberger, Ewa Sikora, Wolfgang Stuetz, Olivier Toussaint, Florence Debacq-Chainiaux, Antti Hervonen, Mikko Hurme, P. Eline Slagboom, Christiane Schoen, Juergen Bernhardt, Nicolle Breusing, Eduardo Pasaro, Ana Maseda, Laura Lorenzo-Lopez, Jose C. Millan-Calenti, Mauro Provinciali, Marco Malavolta
Summary: This study analyzed the association between TTV viremia and physical frailty and cognitive impairment. The results showed that TTV viremia was associated with physical frailty and cognitive impairment. However, the association varied in different study samples.
Review
Environmental Sciences
Maria Joao Bessa, Fatima Brandao, Fernanda Rosario, Luciana Moreira, Ana Teresa Reis, Vanessa Valdiglesias, Blanca Laffon, Sonia Fraga, Joao Paulo Teixeira
Summary: Several studies have investigated the potential adverse health risks associated with exposure to nanoscale materials. In vitro model systems provide important information about the molecular mechanisms of action, while in vivo studies are crucial for understanding the relationship between dose and adverse effects. This review focuses on the toxicity assessment of inhaled (nano)particulate materials and provides an overview of available human respiratory models and exposure systems for in vitro testing, as well as their advantages, limitations, and current research.
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART B-CRITICAL REVIEWS
(2023)
Article
Biotechnology & Applied Microbiology
Marcello Ceppi, Bozena Smolkova, Marta Staruchova, Alena Kazimirova, Magdalena Barancokova, Katarina Volkovova, Andrew Collins, Anton Kocan, Zuzana Dzupinkova, Alexandra Horska, Verona Buocikova, Jana Tulinska, Aurelia Liskova, Miroslava Lehotska Mikusova, Zora Krivosikova, Ladislava Wsolova, Daniel Kuba, Elise Runden-Pran, Naouale El Yamani, Eleonora Martha Longhin, Erika Halasova, Soterios Kyrtopoulos, Stefano Bonassi, Maria Dusinska
Summary: As part of a large human biomonitoring study, occupational monitoring was conducted in a glass fibre factory in Slovakia, comparing the exposure of shopfloor workers and administrators to glass fibres and PAHs. The study investigated the impact of exposure on various genetic factors and found that even low levels of exposure to these fibres can cause significant genetic damage.
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2023)
Article
Biochemical Research Methods
Andrew Collins, Peter Moller, Goran Gajski, Sona Vodenkova, Abdulhadi Abdulwahed, Diana Anderson, Ezgi Eyluel Bankoglu, Stefano Bonassi, Elisa Boutet-Robinet, Gunnar Brunborg, Christy Chao, Marcus S. S. Cooke, Carla Costa, Solange Costa, Alok Dhawan, Joaquin de Lapuente, Cristian Del Bo, Julien Dubus, Maria Dusinska, Susan J. J. Duthie, Naouale El Yamani, Bevin Engelward, Isabel Gaivao, Lisa Giovannelli, Roger Godschalk, Sofia Guilherme, Kristine B. B. Gutzkow, Khaled Habas, Alba Hernandez, Oscar Herrero, Marina Isidori, Awadhesh N. N. Jha, Siegfried Knasmueller, Ingeborg M. M. Kooter, Gudrun Koppen, Marcin Kruszewski, Carina Ladeira, Blanca Laffon, Marcelo Larramendy, Ludovic Le Hegarat, Angelique Lewies, Anna Lewinska, Guillermo E. E. Liwszyc, Adela Lopez de Cerain, Mugimane Manjanatha, Ricard Marcos, Mirta Milic, Vanessa Moraes de Andrade, Massimo Moretti, Damian Muruzabal, Matjaz Novak, Rui Oliveira, Ann-Karin Olsen, Norah Owiti, Mario Pacheco, Alok K. K. Pandey, Stefan Pfuhler, Bertrand Pourrut, Kerstin Reisinger, Emilio Rojas, Elise Runden-Pran, Julen Sanz-Serrano, Sergey Shaposhnikov, Ville Sipinen, Karen Smeets, Helga Stopper, Joao Paulo Teixeira, Vanessa Valdiglesias, Mahara Valverde, Frederique van Acker, Frederik-Jan van Schooten, Marie Vasquez, Johannes F. F. Wentzel, Maciej Wnuk, Annelies Wouters, Bojana Zegura, Tomas Zikmund, Sabine A. S. Langie, Amaya Azqueta
Summary: The comet assay is a versatile method for detecting various types of DNA damage in individual eukaryotic cells. It has been widely used in research, industry, and regulatory agencies, and has been adopted as an in vivo test for genotoxicity. The present document provides consensus protocols for applying the comet assay to different cell types, species, and types of DNA damage.
Article
Environmental Sciences
Sanchez-Alarcon Juana, Milic Mirta, Bonassi Stefano, Gomez-Arroyo Sandra, Cortes- Eslava Josefina, Flores-Marquez Ana Rosa, Valencia- Quintana Rafael Alexander, Valencia-Quintana Rafael
Summary: Mexico is an agriculturally important country with limited biomonitoring data. High pesticide use in horticultural productivity leads to environmental contamination and risks to workers' health. The study shows that horticulturists have significantly higher genetic damage compared to unexposed controls, indicating the need for protective measures and educational programs to prevent health risks.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Genetics & Heredity
Peter Moller, Amaya Azqueta, Julen Sanz-Serrano, Tamara Bakuradze, Elke Richling, Ezgi Eyluel Bankoglu, Helga Stopper, Victoria Claudino Bastos, Sabine A. S. Langie, Annie Jensen, Francesca Scavone, Lisa Giovannelli, Maria Wojewodzka, Marcin Kruszewski, Vanessa Valdiglesias, Blanca Laffon, Carla Costa, Solange Costa, Joao Paulo Teixeira, Mirko Marino, Cristian Del Bo, Patrizia Riso, Congying Zheng, Sergey Shaposhnikov, Andrew Collins
Summary: Measurement of DNA migration in the comet assay can be done through image analysis or visual scoring, with the latter accounting for a significant portion of the published results. This study examines the variability in visual scoring of comets among different investigators and suggests the use of three reference sets for scoring. The results show both inter-investigator and intra-investigator variation in scoring, indicating a need for further standardization, but also demonstrate that visual scoring remains a reliable method for analyzing DNA migration in comets.
Article
Clinical Neurology
Piero Barbanti, Cinzia Aurilia, Gabriella Egeo, Paola Torelli, Stefania Proietti, Sabina Cevoli, Stefano Bonassi, Italian Migraine Registry Study Grp
Summary: In a multicenter study assessing the use of anti-CGRP mAbs in patients with high-frequency or chronic migraine, it was found that half of the nonresponders at 12 weeks actually responded later. Efficacy of the treatment should be evaluated at 24 weeks and treatment duration extended beyond 12 months.
Article
Genetics & Heredity
Peter Moller, Amaya Azqueta, Adriana Rodriguez-Garraus, Tamara Bakuradze, Elke Richling, Ezgi Eyluel Bankoglu, Helga Stopper, Victoria Claudino Bastos, Sabine A. S. Langie, Annie Jensen, Sara Ristori, Francesca Scavone, Lisa Giovannelli, Maria Wojewodzka, Marcin Kruszewski, Vanessa Valdiglesias, Blanca Laffon, Carla Costa, Solange Costa, Joao Paulo Teixeira, Mirko Marino, Cristian Del Bo, Patrizia Riso, Congying Zheng, Sergey Shaposhnikov, Andrew Collins
Summary: Accumulation of DNA strand breaks during cryopreservation is not a concern for mononuclear blood cell lines, and there is inter-laboratory variation in the results.
Article
Genetics & Heredity
Peter Moller, Amaya Azqueta, Adriana Rodriguez-Garraus, Tamara Bakuradze, Elke Richling, Ezgi Eyluel Bankoglu, Helga Stopper, Victoria Claudino Bastos, Sabine A. S. Langie, Annie Jensen, Sara Ristori, Francesca Scavone, Lisa Giovannelli, Maria Wojewodzka, Marcin Kruszewski, Vanessa Valdiglesias, Blanca Laffon, Carla Costa, Solange Costa, Joao Paulo Teixeira, Mirko Marino, Cristian Del Bo', Patrizia Riso, Congying Zheng, Sergey Shaposhnikov, Andrew Collins
Summary: The study finds that potassium bromate is a suitable positive control for the Fpg-modified comet assay, which is widely used to measure oxidatively generated damage to DNA. Although all laboratories obtained positive concentration-response relationships, there was variation in the levels of Fpg-sensitive sites between different laboratories. However, the consistency between the measurements of %Tail DNA values and visual scores of comet images done in the central laboratory suggests a potential source of inter-laboratory variation.
Article
Nanoscience & Nanotechnology
Fatima Brandao, Carla Costa, Maria Joao Bessa, Vanessa Valdiglesias, Bryan Hellack, Andrea Haase, Sonia Fraga, Joao Paulo Teixeira
Summary: This study assessed the cytotoxicity and genotoxicity of different-sized and surface-modified amorphous silica nanomaterials on rat alveolar epithelial cells. The results showed that variants with larger surface areas had higher cytotoxic potential, SiO2_40 induced significant DNA damage, and all tested variants increased gamma-H2AX levels. This study supports the use of multiparametric approaches to improve understanding of nanomaterial mechanisms and hazard prediction.
Article
Environmental Sciences
Mahara Valverde, Adriana Granados, Mirta Milic, Marcello Ceppi, Leticia Sollano, Stefano Bonassi, Emilio Rojas
Summary: DNA damage in newborn children's cord blood cells from Mexico City is correlated with the DNA damage in their mothers, as well as the environmental exposure and complications during pregnancy. Mothers living in the southern part of the city have double DNA damage compared to those living in the northern part. This study highlights the importance of antioxidants during pregnancy and the role of the placental barrier in protecting newborns from DNA-damaging effects of pollution.
Article
Clinical Neurology
Piero Barbanti, Gabriella Egeo, Cinzia Aurilia, Paola Torelli, Cinzia Finocchi, Florindo d'Onofrio, Luigi d'Onofrio, Renata Rao, Stefano Messina, Laura Di Clemente, Angelo Ranieri, Massimo Autunno, Giuliano Sette, Bruno Colombo, Antonio Carnevale, Marco Aguggia, Miriam Tasillo, Francesco Zoroddu, Fabio Frediani, Massimo Filippi, Carlo Tomino, Stefania Proietti, Stefano Bonassi
Summary: This study confirms the long-term efficacy, safety, and tolerability of fremanezumab in real-life patients with high-frequency episodic migraine and chronic migraine. The drug significantly reduced the frequency of migraines and headaches during the 24-week treatment period, with minimal side effects.
JOURNAL OF HEADACHE AND PAIN
(2023)
Review
Biotechnology & Applied Microbiology
Francisco Carlos da Silva Junior, Leticya Pinto de Araujo, Joao Paulo de Mendonca Freitas, Nilmara de Oliveira Alves, Stefano Bonassi, Silvia Regina Batistuzzo de Medeiros
Summary: This study conducted a systematic review and meta-analysis on the association between ambient particulate matter (PM) exposure and micronucleus (MN) frequency. The results showed a significant association between PM exposure and MN frequency, with buccal cells and low economic status as potential effect modifiers. This research is important for understanding the impact of environmental pollution on human health.
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
(2023)
Article
Nutrition & Dietetics
Marco Crocco, Angela Calvi, Francesca Canzoneri, Federica Malerba, Noemi Zampatti, Andrea Chiaro, Serena Arrigo, Paolo Gandullia, Stefania Proietti, Stefano Bonassi
Summary: This study assessed the impact of the COVID-19 pandemic on the number of new-onset celiac disease cases, and found a 14.5% decrease in cases during the pandemic compared to previous years. This is the first study to evaluate the effect of COVID-19 on celiac disease diagnosis in children.
Article
Medicine, General & Internal
Walter Marrocco, Antonella Galli, Silvestro Scotti, Nicola Calabrese, Paolo Misericordia, Alessandro Dalle Vedove, Gianmarco Marrocco, Antonio Pio D'Ingianna, Andrea Pizzini, Massimo Fini, Carlo Tomino, Stefano Bonassi
Summary: This randomized clinical trial conducted by general practitioners evaluated the effects of a multicomponent intervention for preventing falls in elderly individuals at high risk. The intervention group received counseling, risk assessment, physical activity programs, and nutritional counseling, which resulted in fewer falls compared to the control group. However, there was a small reduction in the number of fallers and an unexpected increase in the risk of fractures in the intervention group.
JOURNAL OF CLINICAL MEDICINE
(2023)
