Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution

Single-site substitutions improve cold activity and increase thermostability of the dehairing alkaline protease (DHAP)

(2016) Hong-Yan Zhao et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Defying the activity–stability trade-off in enzymes: taking advantage of entropy to enhance activity and thermostability

(2016) Khawar Sohail Siddiqui   CRITICAL REVIEWS IN BIOTECHNOLOGY

Single amino acid mutation alters thermostability of the alkaline protease from Bacillus pumilus: thermodynamics and temperature dependence

(2014) R. Huang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information

(2014) Marco Biasini et al.   NUCLEIC ACIDS RESEARCH

Detergent-Compatible Proteases: Microbial Production, Properties, and Stain Removal Analysis

(2014) Francois Niyongabo Niyonzima et al.   PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

Engineering of Flexible Loops in Enzymes

(2014) Bettina M. Nestl et al.   ACS Catalysis

Protein Thermostabilizing Factors: High Relative Occurrence of Amino Acids, Residual Properties, and Secondary Structure Type in Different Residual State

(2013) Seung Pil Pack et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

A single mutation within a Ca2+ binding loop increases proteolytic activity, thermal stability, and surfactant stability

(2013) Mitsuyoshi Okuda et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Gly or Ala substitutions for Pro210Thr211Asn212 at the β8–β9 turn of subtilisin Carlsberg increase the catalytic rate and decrease thermostability

(2012) Naoki Fuchita et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Increasing activity and thermal resistance ofBacillus gibsoniialkaline protease (BgAP) by directed evolution

(2012) Ronny Martinez et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cloning, expression and decoding of the cold adaptation of a new widely represented thermolabile subtilisin-like protease

(2012) J.P. Acevedo et al.   JOURNAL OF APPLIED MICROBIOLOGY

A Simple and Reproducible Method for Directed Evolution: Combination of Random Mutation with dITP and DNA Fragmentation with Endonuclease V

(2012) Zun Wang et al.   MOLECULAR BIOTECHNOLOGY

Enhancement of the thermostability and the catalytic efficiency of Bacillus pumilus CBS protease by site-directed mutagenesis

(2010) Bassem Jaouadi et al.   BIOCHIMIE

Effects of the mutational combinations on the activity and stability of thermolysin

(2010) Masayuki Kusano et al.   JOURNAL OF BIOTECHNOLOGY

Substrate Specificity and Thermostability of the Dehairing Alkaline Protease from Bacillus pumilus

(2009) Min-Yuan Wan et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Improvement of low-temperature caseinolytic activity of a thermophilic subtilase by directed evolution and site-directed mutagenesis

(2009) Chuan-Qi Zhong et al.   BIOTECHNOLOGY AND BIOENGINEERING

Biochemical and molecular characterization of a detergent-stable serine alkaline protease from Bacillus pumilus CBS with high catalytic efficiency

(2008) Bassem Jaouadi et al.   BIOCHIMIE

Cold-adapted maturation of thermophilic WF146 protease by mimicking the propeptide binding interactions of psychrophilic subtilisin S41

(2008) Yi-Ran Yang et al.   FEBS LETTERS

Effect of Proline Substitutions on Stability and Kinetic Properties of a Cold Adapted Subtilase

(2008) J. Arnorsdottir et al.   JOURNAL OF BIOCHEMISTRY