Article
Food Science & Technology
Ummuhan Cakmak, Fulya Oz Tuncay, Yakup Kolcuoglu
Summary: The amylase obtained from Cladophora hutchinsiae was purified and characterized in terms of its properties and applications. The enzyme showed optimal activity at pH 6 and 10°C, and demonstrated remarkable pH stability. It could be used in the presence of certain organic solvents and cations. Additionally, the enzyme was effective in releasing reducing sugar from fruit juices and fish feed, suggesting its potential applications in various industries.
Article
Multidisciplinary Sciences
S. Soy, U. Lakra, P. Prakash, P. Suravajhala, V. K. Nigam, S. R. Sharma, N. Bayal
Summary: This study investigated the microbial diversity of hot springs and identified a thermophilic isolate with potential for degrading lignocellulosic agricultural waste. The isolate's enzyme production ability was analyzed and characterized.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Xiaodong Zhang, Caixia Li, Xuantong Chen, Chonlong Chio, Sarita Shrestha, Wensheng Qin
Summary: The study identified and characterized a high alpha-amylase producing bacterium WangLB, and cloned its alpha-amylase coding gene. The characterized BvAmylase showed good stability and activity, making it a potential candidate enzyme for industrial applications.
FERMENTATION-BASEL
(2021)
Article
Chemistry, Physical
Scheherazed Dakhmouche Djekrif, Leila Bennamoun, Fatima Zohra Kenza Labbani, Amel Ait Kaki, Tahar Nouadri, Andre Pauss, Zahia Meraihi, Louisa Gillmann
Summary: The study showed that alpha-amylase and pullulanase from Clavispora lusitaniae ABS7 isolated from wheat seeds exhibited high activity and stability, making them suitable for industrial applications such as detergents.
Article
Plant Sciences
Subhadip Paul, Amitava Rakshit
Summary: The study investigated the impact of seed bio-priming with Trichoderma viride on degradation of stored foods, phenol content, and nutrient absorption in germinating soybean. The activities of various enzymes were measured to assess hydrolysis of starch, lipid, and protein. Bio-primed seeds showed faster degradation of lipid and starch, as well as higher protein hydrolysis. The phenol content in bio-primed cotyledons reduced lipid peroxidation and exhibited stronger radical scavenging activity. Higher potassium content was observed in bio-primed cotyledons, while nitrogen and phosphorus concentrations were significantly higher on day 5. These factors contributed to faster and more vigorous germination of bio-primed soybean seeds compared to non-primed ones.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
E. Ataallahi, H. Naderi-Manesh, R. Roostaazad, S. Yeganeh
Summary: Purification of extracellular alpha-amylase from Bacillus subtilis was achieved through fractional precipitation by acetone and ion exchange chromatography, resulting in improved enzyme purity, activity, and stability. The optimization of operation parameters using response surface methodology was crucial in achieving high yield and purification fold.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Jincheng Li, Sen Liu, Chenjie Yang, Nemat O. Keyhani, Huili Pu, Longbin Lin, Xiaoxia Li, Peisong Jia, Dongmei Wu, Jieming Pan, Philip C. Stevenson, G. Mandela Fernandez-Grandon, Liaoyuan Zhang, Yuxi Chen, Xiayu Guan, Junzhi Qiu
Summary: The insect pathogenic fungus, Ascosphaera apis, secretes a novel amylase, which shows high activity and stability under specific conditions. The production of amylase by A. apis was optimized using the response surface method, and the purified enzyme exhibited thermostability and broad pH range activity. The activity of the enzyme was significantly affected by certain metal ions and solvents.
Article
Engineering, Chemical
Mona Alonazi, Aida Karray, Ahmed Yacine Badjah-Hadj-Ahmed, Abir Ben Bacha
Summary: The study identified a marine bacterial amylase produced by Bacillus pacificus associated with Turbinaria ornata. The newly purified amylase showed potential for biotechnological applications, generating maltohexaose from starch. It exhibited high activity on Alylose and Alylopectine, and was stable under high temperature and alkaline conditions.
Article
Chemistry, Applied
Qing-Qing Lv, Juan-Juan Cao, Rui Liu, Han-Qing Chen
Summary: Two polysaccharide fractions, WXA-1 and AXA-1, were isolated from wheat bran in this study. Structural analysis revealed that they are heteropolysaccharides, with AXA-1 showing stronger inhibitory effects on enzyme activities compared to WXA-1.
Article
Engineering, Chemical
Mauro Marengo, Davide Pezzilli, Eleonora Gianquinto, Alex Fissore, Simonetta Oliaro-Bosso, Barbara Sgorbini, Francesca Spyrakis, Salvatore Adinolfi
Summary: Alpha-amylases are enzymes with a wide distribution and high sequence identity, but their catalytic activities have diverged throughout evolution. By comparing alpha-amylases from human, Aspergillus oryzae, and pancreatic porcine, we found that despite structural homology, these enzymes exhibit strikingly different activities mediated by different ions. This highlights the need to carefully consider these functional differences and establish proper experimental conditions when using alpha-amylases from different organisms as models for human enzymes.
Article
Chemistry, Multidisciplinary
Asma Zafar, Ziaur Rahman, Hira Mubeen, Javairia Makhdoom, Javeria Tariq, Mahjabeen, Zulqurnain Ali, Attia Hamid, Eeza Shafique, Muhammad Nauman Aftab
Summary: This study aimed to clone and express the esterase gene from Bacillus thuringiensis and investigate its purification, stability, and activity. The results showed that the recombinant esterase enzyme had high activity, stability, and resistance, which are significant for the detergent industry.
Article
Engineering, Environmental
Mohamed Hosny, Manal Fawzy, Esmail M. El-Fakharany, Ahmed M. Omer, Eman M. Abd El-Monaem, Randa E. Khalifa, Abdelazeem S. Eltaweil
Summary: The aqueous extract of P. salicifolium leaves was used to biogenically synthesize platinum nanoparticles (PS-PtNPs) with high stability and catalytic, antibacterial, and antioxidant properties. The synthesized PS-PtNPs showed promising potential for various applications, highlighting a green and cost-effective method for PtNP synthesis.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Microbiology
Shraddha Shaligram, Nitin P. Narwade, Shreyas Kumbhare, Manobjyoti Bordoloi, Kashyap J. Tamuli, Shyamalendu Nath, T. Parimelazhagan, Vikas S. Patil, Atya Kapley, Shrikant P. Pawar, Dhiraj P. Dhotre, M. G. Muddeshwar, Hemant J. Purohit, Yogesh S. Shouche
Summary: This study characterized the anti-diabetic activity of a bacterial isolate SW1 from an effluent treatment plant, revealing its production of acarbose. Through chemical analysis and genomic mining, the potential medicinal value of bacteria from an unconventional habitat was uncovered.
CURRENT MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yu Meng, Zhuang Yao, Huong Giang Le, Se Jin Lee, Hye Sung Jeon, Ji Yeon Yoo, Jeong Hwan Kim
Summary: The cellulolytic gene aprEHJ4 was identified in Bacillus licheniformis HJ4, showing significant fibrinolytic activity, and its expression was found to be highest in a 0% salt culture, followed by 8% and 5% salt cultures. The enzyme's optimal pH and temperature were determined to be pH 8 and 40 degrees Celsius, respectively.
FOLIA MICROBIOLOGICA
(2021)
Article
Biotechnology & Applied Microbiology
Alshaimaa M. M. Elsayed, Mohamed Mahmoud, Ghada S. A. Abdel Karim, Mohamed Abdelraof, Abdelmageed M. M. Othman
Summary: Two laccase isoenzymes (LacA and LacB) were isolated and purified from Trichoderma harzianum S7113. LacA had a molecular weight of 63 kDa, while LacB had a molecular weight of 48 kDa. Both isoenzymes exhibited optimum activity at 50℃, but at slightly different pH values. They also showed similar thermal and pH stability. LacA and LacB had high specific activity toward ABTS, with LacB having a lower K-m and V-max value than LacA. The catalytic activity of both isoenzymes was affected by various ions, and completely inhibited by sodium azide.
MICROBIAL CELL FACTORIES
(2023)
Article
Chemistry, Analytical
Hani A. Alhadrami, Ahmed M. Hassan, Raja Chinnappan, Hind Al-Hadrami, Wesam H. Abdulaal, Esam Azhar, Mohammed Zourob
Summary: In this study, a specific, sensitive, quantitative, and fast detection method for SARS-CoV-2 using the FRET assay was developed, utilizing viral protease proteolytic activity as a biomarker. The method showed a high fluorescence signal with a low detection limit and specificity against MERS-CoV, indicating its potential application for diagnosing SARS-CoV-2 patients.
Article
Microbiology
Sawsan S. Alamri, Khalid A. Alluhaybi, Rowa Y. Alhabbab, Mohammad Basabrain, Abdullah Algaissi, Sarah Almahboub, Mohamed A. Alfaleh, Turki S. Abujamel, Wesam H. Abdulaal, M-Zaki ElAssouli, Rahaf H. Alharbi, Mazen Hassanain, Anwar M. Hashem
Summary: The study successfully engineered a synthetic DNA vaccine against SARS-CoV-2, which induced long-lasting immune responses in mouse models. Using a needle-free system enhanced immunogenicity and reduced the dose required for protective immunity, supporting further preclinical and clinical testing of this candidate vaccine.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Khaled M. Hosny, Rasha A. Khallaf, Hani Z. Asfour, Waleed Y. Rizg, Nabil A. Alhakamy, Amal M. Sindi, Hala M. Alkhalidi, Walaa A. Abualsunun, Rana B. Bakhaidar, Alshaimaa M. Almehmady, Wesam H. Abdulaal, Muhammed A. Bakhrebah, Mohammed S. Alsuabeyl, Ahmed K. Kammoun, Adel F. Alghaith, Sultan Alshehri
Summary: The study aimed to develop a cinnamon oil-loaded nanoemulsion gel to combat oral microbiota and improve oral health. Results showed that the gel was effective in relieving pain and enhancing overall oral health.
Article
Pharmacology & Pharmacy
Nabil A. Alhakamy, Mohammed W. Al-Rabia, Shadab Md, Alaa Sirwi, Selwan Saud Khayat, Sahar Saad AlOtaibi, Raghad Abkar Hakami, Hadeel Al Sadoun, Basmah Medhat Eldakhakhny, Wesam H. Abdulaal, Hibah M. Aldawsari, Shaimaa M. Badr-Eldin, Mahmoud A. Elfaky
Summary: The study developed spanlastics nanotechnology to improve the therapeutic efficacy of luliconazole, enhancing its skin penetration and antifungal activity. The optimized formulation showed significant antifungal efficacy in experimental Candida-infected animals, nearly completely removing fungal colonies on the skin.
Article
Immunology
Khalid A. Alluhaybi, Rahaf H. Alharbi, Rowa Y. Alhabbab, Najwa D. Aljehani, Sawsan S. Alamri, Mohammad Basabrain, Rehaf Alharbi, Wesam H. Abdulaal, Mohamed A. Alfaleh, Levi Tamming, Wanyue Zhang, Mazen Hassanain, Abdullah Algaissi, Adel M. Abuzenadah, Xuguang Li, Anwar M. Hashem
Summary: The study developed and evaluated a DNA vaccine encoding SARS-CoV-2 S1, which demonstrated high levels of specific IgG and neutralizing antibodies in mice. It was also found that a 50 μg dose of pVAX-S1 could elicit significant memory CD4(+) and CD8(+) T cell responses.
Review
Microbiology
Efaq Noman, Muhanna Mohammed Al-Shaibani, Muhammed Adnan Bakhrebah, Reyad Almoheer, Mohammed Al-Sahari, Adel Al-Gheethi, Radin Maya Saphira Radin Mohamed, Yaaser Qaeed Almulaiky, Wesam Hussain Abdulaal
Summary: The fungi from marine environment have promising potential as a source of anticancer agents due to their ability to produce multiple compounds and enzymes that can effectively inhibit cancer cell growth. L-asparaginase degrades the main substance of cancer cells, asparagine, while secondary metabolites produced by fungi can change cell morphology and induce DNA fragmentation, leading to cancer cell apoptosis.
Article
Biochemistry & Molecular Biology
Faisal A. Alzahrani, Mohammed Razeeth Shait Mohammed, Saleh Alkarim, Esam I. Azhar, Mohammed A. El-Magd, Yousef Hawsawi, Wesam H. Abdulaal, Abdulaziz Yusuf, Abdulaziz Alhatmi, Raed Albiheyri, Burhan Fakhurji, Bassem Kurdi, Tariq A. Madani, Hassan Alguridi, Roaa S. Alosaimi, Mohammad Imran Khan
Summary: This study investigated the metabolite content of EVs isolated from the serum of COVID-19 patients, revealing the presence of antivirals, antibiotics, anti-inflammatory metabolites, and decreased levels of coagulation-related metabolites. These findings suggest that EVs may play a crucial role in mediating heightened inflammation during COVID-19 infection, paving the way for the identification of novel metabolites as regulators of inflammatory pathways during viral infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Nabil A. Alhakamy, Usama A. Fahmy, Shaimaa M. Badr Eldin, Osama A. A. Ahmed, Hibah M. Aldawsari, Solomon Z. Okbazghi, Mohamed A. Alfaleh, Wesam H. Abdulaal, Abdulmohsin J. Alamoudi, Fatma M. Mady
Summary: This study demonstrates that incorporating quercetin, scorpion venom peptides, and Phospholipon(R) 90H into a nano-based delivery platform improves the cellular uptake and antiproliferative efficacy of quercetin, showing potential as a therapeutic approach for the treatment of breast cancer.
Review
Medicine, General & Internal
Omar Fahmy, Nabil A. Alhakamy, Waleed Y. Rizg, Alaa Bagalagel, Abdulmohsin J. Alamoudi, Hibah M. Aldawsari, Aiah M. Khateb, Basmah M. Eldakhakhny, Usama A. Fahmy, Wesam H. Abdulaal, Claudia G. Fresta, Giuseppe Caruso
Summary: Understanding the mechanisms underlying the development and progression of prostate cancer is crucial for maximizing the benefit of current medications and guiding future research for targeted therapy. Future treatment strategies may involve predictive genetic and biomarker analysis to assign patients to the most appropriate and effective treatment.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Nabil A. Alhakamy, Shaimaa M. Badr-Eldin, Osama A. A. Ahmed, Hibah M. Aldawsari, Solomon Z. Okbazghi, Mohamed A. Alfaleh, Wesam H. Abdulaal, Thikryat Neamatallah, Omar D. Al-hejaili, Usama A. Fahmy
Summary: The study demonstrates that a nanoemulsion formulation of costunolide (CTD) could be a potential therapeutic approach for lung cancer treatment. The optimized CTD nanoemulsion inhibited cell proliferation and promoted apoptosis in lung cancer cells. Additionally, the formulation reduced inflammatory responses, suggesting its significance in lung cancer therapy.
Article
Pharmacology & Pharmacy
Nabil A. Alhakamy, Giuseppe Caruso, Basma G. Eid, Usama A. Fahmy, Osama A. A. Ahmed, Ashraf B. Abdel-Naim, Abdulmohsin J. Alamoudi, Shareefa A. Alghamdi, Hadeel Al Sadoun, Basmah M. Eldakhakhny, Filippo Caraci, Wesam H. Abdulaal
Summary: The study investigates the potential of a nanoconjugate of CTX and MEL loaded in hydroxypropyl methylcellulose gel to improve wound healing in diabetic rats. The results show that the nanocomplex enhances epithelium regeneration, reduces oxidative stress, decreases pro-inflammatory cytokines, and increases collagen expression, highlighting its promising application in wound healing research.
Article
Microbiology
Momen Askoura, Hisham A. Abbas, Hadeel Al Sadoun, Wesam H. Abdulaal, Amr S. Abu Lila, Khaled Almansour, Farhan Alshammari, El-Sayed Khafagy, Tarek S. Ibrahim, Wael A. H. Hegazy
Summary: This study investigated the role of IL-33, IL-17, and IL-25 in patients with hepatitis C virus (HCV) and the progression of the disease from chronicity to hepatocellular carcinoma. The results showed that the serum levels of IL-33 and IL-17 significantly increased in patients with chronic hepatitis C and hepatocellular carcinoma. IL-33 was also found to be associated with liver fibrosis progression and viral load, while IL-17 and IL-25 were mainly related to the development of hepatocellular carcinoma.
Article
Biochemistry & Molecular Biology
Hani A. Alhadrami, Ahmed M. Sayed, Sami A. Melebari, Asem A. Khogeer, Wesam H. Abdulaal, Mohamed B. Al-Fageeh, Mohammad Algahtani, Mostafa E. Rateb
Summary: Recent research has identified several compounds derived from plants that can inhibit human aromatase, with these compounds showing stronger inhibitory effects on ER+ cells. Additionally, the mechanisms of inhibition for these compounds are competitive and non-competitive, respectively.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Khaled M. Hosny, Amal M. Sindi, Hala M. Alkhalidi, Mallesh Kurakula, Nabil K. Alruwaili, Nabil A. Alhakamy, Walaa A. Abualsunun, Rana B. Bakhaidar, Rahaf H. Bahmdan, Waleed Y. Rizg, Sarah A. Ali, Wesam H. Abdulaal, Majed S. Nassar, Mohammed S. Alsuabeyl, Adel F. Alghaith, Sultan Alshehri
Summary: This study aimed to combine the benefits of penciclovir and lavender oil in the form of a self-nanoemulsifying drug delivery system for the treatment of herpes labialis. The optimized formulation demonstrated significantly higher and sustained penciclovir release and a relative bioavailability of 180% compared to a marketed 1% penciclovir cream. Overall, the results confirm that the optimized formulation can provide an efficient delivery system for penciclovir to reach oral mucosa and achieve prolonged release.
Article
Hematology
Basmah Eldakhakhny, Hadeel Al Sadoun, Nehal Bin Taleb, Dunya Ahmed Nori, Nawal Helmi, Iffat Mirza Ahmed, Muhammed A. Bakhrebah, Wesam H. Abdulaal
Summary: The study found that CD47 expression is significantly decreased and apoptosis is increased in red blood cells of sickle cell disease (SCD) patients. SCD patients showed higher levels of pro-inflammatory cytokines IL-6 and IL-1 beta, with IL-1 beta inversely correlated with SIRP alpha expression.
JOURNAL OF HEMATOPATHOLOGY
(2021)
