Article
Chemistry, Inorganic & Nuclear
Roberta Sgammato, Niels Van Brempt, Roy Aerts, Sabine Van Doorslaer, Sylvia Dewilde, Wouter Herrebout, Christian Johannessen
Summary: Protoglobin from Methanosarcina acetivorans (MaPgb) is a dimeric globin belonging to the same lineage as globin-coupled sensors. It has a putative role in scavenging reactive nitrogen and oxygen species. Through various spectroscopic techniques, the unusual reaction of ferric MaPgb with nitrite was revealed. Unlike other globins, nitrite did not induce the formation of a nitriglobin form in MaPgb. Instead, it led to the formation of a stable nitric-oxide ligated ferric form of the protein (MaPgb-NO) in mildly acidic pH. The changes in the optical and vibrational properties of MaPgb upon nitric-oxide ligation provide insights into its gas-binding mechanism.
DALTON TRANSACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Jack Silver, Golzar al-Jaff, Jehad A. Taies, Michael T. Wilson, Daniel den Engelsen, George R. Fern, Terry G. Ireland
Summary: The visible and Mossbauer spectra of [Fe(II)(Por)L-2] and [Fe(II)(Por)L(CO)] complexes were analyzed and compared with other complexes and hemoglobin and myoglobin. A new approach was developed to extract information from the Mossbauer parameters, revealing a significant dependence on the porphyrin and nitrogenous ligand. Different trend lines were found for aromatic and aliphatic nitrogenous ligands, indicating different charge donation abilities of the porphyrins. The plots of the Mossbauer parameters against bond lengths provided further understanding of the bonding geometry in CO hemoglobin and CO myoglobin.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Kiran Kumar Adepu, Dipendra Bhandari, Andriy Anishkin, Sean H. Adams, Sree Chintapalli
Summary: This study reveals the affinity of lactate to myoglobin and its effect on the release of oxygen. These findings suggest that lactate may act as a regulator of oxygen management in tissues rich in myoglobin and influence the signaling roles of oxygenated and deoxygenated myoglobin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Yu-Qi Wang, Xiao-Han Dan, Xiang Wang, Zhen-Yu Yi, JiaJu Fu, Ya-Chen Feng, Jin-Song Hu, Dong Wang, Li-Jun Wan
Summary: We report an in situ electrochemical scanning tunneling microscopy (ECSTM) study on the synergistic effect of Mg2+ in the CO2 reduction reaction (CO2RR) catalyzed by cobalt phthalocyanine (CoPc). Our study reveals that Mg2+ enhances the adsorption of CO2 on CoPc, resulting in the formation of CoPc-CO2-Mg2+ complexes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biology
Takeshi Imai, Ryuta Tobe, Koji Honda, Mai Tanaka, Jun Kawamoto, Hisaaki Mihara
Summary: This study reveals that a truncated haemoglobin, YjbI, from Bacillus subtilis prevents oxidative aggregation of cell-surface proteins by its peroxidase-like activity, removing hydroperoxide groups from oxidised proteins. Disruption of the yjbI gene leads to decreased biofilm water repellence and cross-linked aggregation of the biofilm matrix protein TasA. YjbI plays a pleiotropic role in protecting labile cell-surface proteins from oxidative damage in bacteria.
Article
Chemistry, Multidisciplinary
Izumi Ishigami, Sergio Carbajo, Nadia Zatsepin, Masahide Hikita, Chelsie E. Conrad, Garrett Nelson, Jesse Coe, Shibom Basu, Thomas Grant, Matthew H. Seaberg, Raymond G. Sierra, Mark S. Hunter, Petra Fromme, Raimund Fromme, Denis L. Rousseau, Syun-Ru Yeh
Summary: Cytochrome c oxidase (CcO) is a hemeprotein that reduces dioxygen to water. It has a unique structure with a binuclear center composed of a copper atom and a heme iron. In the CO complex of CcO, the CO molecule dissociates from the heme iron and moves to a temporary binding site between the copper atom and the heme iron. This new structure provides insight into the ligand dissociation trajectory and protein dynamics.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Inorganic & Nuclear
Avisikta Sinha, Ankit Kumar, Mangalampalli Ravikanth
Summary: An aromatic tricoordinated organo B(III) complex was synthesized by reacting a non-aromatic phlorin analogue with BCl3, showing trigonal-planar geometry with coordination to two nitrogen atoms and one carbon. Spectral and theoretical studies supported the aromatic nature of the B(III) complex, which acts as a specific ion sensor.
INORGANIC CHEMISTRY
(2021)
Article
Chemistry, Physical
Camila Bacellar, Dominik Kinschel, Oliviero Cannelli, Boris Sorokin, Tetsuo Katayama, Giulia F. Mancini, Jeremy R. Rouxel, Yuki Obara, Junichi Nishitani, Hironori Ito, Terumasa Ito, Naoya Kurahashi, Chika Higashimura, Shotaro Kudo, Claudio Cirelli, Gregor Knopp, Karol Nass, Philip J. M. Johnson, Anna Wach, Jakub Szlachetko, Frederico A. Lima, Christopher J. Milne, Makina Yabashi, Toshinori Suzuki, Kazuhiko Misawa, Majed Chergui
Summary: The study discusses ligand dissociation and recombination in MbNO, as well as preliminary findings of femtosecond X-ray absorption studies pointing to the presence of multiple species formed upon photolysis.
FARADAY DISCUSSIONS
(2021)
Article
Biology
Naim M. Bautista, Christian Damsgaard, Angela Fago, Tobias Wang
Summary: This study demonstrates that during diving, crocodilians primarily accumulate CO2 within erythrocytes, where most of it binds to hemoglobin. The binding of HCO3- is tightly associated with the progressive blood deoxygenation during diving, showing a difference from the classic vertebrate pattern.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Polymer Science
Brian F. Patenaude, Erik B. Berda, Samuel Pazicni
Summary: A series of zinc porphyrin-cored random coil polymers and polymeric nanoparticles were synthesized and characterized to study secondary coordination sphere interactions. The study showed that polymeric nanoparticles with a greater number of potential hydrogen bond donors and greater steric bulk catalyzed the reaction of cyanide with N,N-dimethylformamide at the highest reaction rates.
Article
Chemistry, Organic
Avisikta Sinha, Mangalampalli Ravikanth
Summary: The benzofuran-embedded selena- and telluraporphyrins were synthesized through replacing the pyrrole ring with a benzofuran unit, resulting in altered electronic properties. The presence of the benzofuran moiety in the macrocycles led to distorted structures and hindered pi-electron delocalization. The absorption spectra showed strong bands in the visible range for the benzofuran-embedded selena- and telluraporphyrins, while the protonated derivatives exhibited strong absorption in the near-infrared region.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Qiyue Mao, Pradip K. Das, Stephane Le Gac, Bernard Boitrel, Vincent Dorcet, Koji Oohora, Takashi Hayashi, Hiroaki Kitagishi
Summary: Fe(III)Por-1 was synthesized to mimic the function of myoglobin in aqueous solution, forming a stable inclusion complex with Py3OCD. Fe(II)PorCD-1 and Fe(II)PorCD-2 showed differences in O2 and CO binding, with the strapped structure hindering the entrance of gaseous ligands into the iron(II) center.
INORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Dayna C. Patterson, Yilin Liu, Sayan Das, Neela H. Yennawar, Jean-Paul Armache, James R. Kincaid, Emily E. Weinert
Summary: The bifunctional enzyme DcpG can selectively regulate its two metabolic domains in response to heme gaseous ligands. Mutations of heme-edge residues alter the protein conformation, leading to changes in signaling transduction and enzyme kinetics. These findings provide insights into the molecular interactions that regulate DcpG gas sensing and mechanisms that control multidomain bacterial signaling proteins.
Article
Hematology
Fanbi Meng, Shuang Chen, Chunliang Liu, Muhammad Shoaib Khan, Yan Yan, Jun Wan, Yue Xia, Chenglin Sun, Mengnan Yang, Renping Hu, Kesheng Dai
Summary: PKC plays a critical role in regulating megakaryocyte development and platelet production in thrombocytopenia. The inhibition of PKC activation delays and reduces radiation-induced megakaryocyte apoptosis, leading to improved platelet count recovery. This study provides a potential therapeutic target for treating thrombocytopenia.
BLOOD CELLS MOLECULES AND DISEASES
(2024)
Article
Hematology
Xia Chen, Fang Liu, Yuanyuan Ren, Luyang Zhang, Yang Wan, Wenyu Yang, Xiaojuan Chen, Li Zhang, Yao Zou, Yumei Chen, Xiaofan Zhu, Ye Guo
Summary: Unrelated umbilical cord blood transplantation (UCBT) is an effective alternative treatment for pediatric patients with bone marrow failure. The use of the TBI/BU + FLU + CY conditioning regimen ensures a high engraftment rate for UCBT and overcomes the challenge of graft failure. Secondary salvage use of cord blood transplantation may still be useful for patients who have failed after other transplantation.
BLOOD CELLS MOLECULES AND DISEASES
(2024)
Article
Hematology
Chithra D. Palani, Xingguo Zhu, Manickam Alagar, Otis C. Attucks, Betty S. Pace
Summary: This study evaluated the ability of a novel Bach1 inhibitor, HPP-D, to induce HbF in KU812 cells and primary sickle erythroid progenitors. The results showed that HPP-D increased HbF levels and decreased Bach1 protein levels in both cell types. Furthermore, it enhanced NRF2 binding to the gamma-globin promoter antioxidant response elements, leading to an open chromatin configuration and increased gamma-globin transcription.
BLOOD CELLS MOLECULES AND DISEASES
(2024)
Letter
Hematology
Marcos Borato Viana, Erica Louback Oliveira, Andre Rolim Belisario
BLOOD CELLS MOLECULES AND DISEASES
(2024)
Article
Hematology
Ke Zhang, Longying Ye, Yanhui Jin, Yuan Chen, Shuting Jiang, Haixiao Xie, Lihong Yang, Mingshan Wang
Summary: This study investigated two pedigrees with FV deficiency and identified three mutations that may have deleterious effects on the function and structure of FV.
BLOOD CELLS MOLECULES AND DISEASES
(2024)
