Article
Hematology
Danielle Karakas, Miao Xu, Heyu Ni
Summary: Thrombopoietin (TPO) plays important roles in platelet generation, with recent studies showing that platelets can induce TPO production in the liver in addition to clearing it. The platelet glycoprotein Ib alpha was found to be essential for platelet-mediated TPO generation.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Pharmacology & Pharmacy
Ahmed E. Hamouda, Kai Schulze, Thomas Ebensen, Carlos Alberto Guzman, Dario Lirussi
Summary: Cyclic di-AMP (CDA) and di-GMP (CDG) are promising immune modulators that induce immune response in vaccination and cancer immunotherapy. The signaling of type I interferon (IFN-alpha/beta) is crucial for the activation and enhanced immunogenicity mediated by CDG and CDA.
Review
Oncology
Gerd Bendas, Martin Schlesinger
Summary: This review summarizes the recent advances in GPIb-IX biology, including its structure, functions, ligands, and signaling cascades, as well as its regulatory roles in platelet production, clearance, and thrombopoietin secretion. Furthermore, the involvement of GPIb in liver cancer development and metastasis is also discussed.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sergey Okhota, Sergey Kozlov, Yuliya Avtaeva, Ivan Melnikov, Olga Saburova, Konstantin Guria, Evgeny Matroze, Zufar Gabbasov
Summary: This study examined platelet adhesion mediated by von Willebrand factor (VWF) at high shear rates in patients with premature coronary artery disease (CAD). The researchers found that patients with premature CAD exhibited a greater GPIb-mediated component of platelet adhesion compared to control patients. This suggests a potential role of excessive VWF-platelet interactions in the development of premature CAD.
Editorial Material
Hematology
Sufia N. Amini, Vivianne S. Nelson, Leendert Porcelijn, Tanja Netelenbos, Jaap Jan Zwaginga, Masja de Haas, Martin R. Schipperus, Rick Kapur
Summary: ITP is an autoimmune bleeding disorder characterized by platelet clearance in the spleen and liver. Thrombopoietin (TPO) levels remain low in ITP. The role of GPIb antibodies in relation to platelet hepatic sequestration and TPO levels in ITP remains unclear. This study found a positive association between GPIb antibodies and TPO levels as well as platelet hepatic sequestration in patients with severe thrombocytopenic ITP.
Article
Engineering, Biomedical
Peineng Wang, Jawaad Sheriff, Peng Zhang, Yuefan Deng, Danny Bluestein
Summary: In this study, a multiscale model was developed to simulate the adhesive dynamics of platelets to blood vessel walls under physiological flow shear stresses. The binding of platelet glycoprotein receptor Iba to von Willebrand factor on the blood vessel wall was modeled using a molecular-level hybrid force field and validated with in vitro experiments. The simulation results revealed the molecular bonding and debonding processes during platelet adhesion, as well as the distribution of bond strength, providing a biomechanical insight into the initiation of this complex process.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Hematology
Jeanne F. Rivera, April J. Baral, Fatima Nadat, Grace Boyd, Rachael Smyth, Hershna Patel, Emma L. Burman, Ghadah Alameer, Sally A. Boxall, Brian R. Jackson, E. Joanna Baxter, Peter Laslo, Anthony R. Green, David G. Kent, Ann Mullally, Edwin Chen
Summary: This study identified key motifs for the oncogenic activity of CALR(de)(l52), highlighting the essential role of the zinc-binding domain in multimerization of CALR(de)(l52) and interaction with MPL. Zinc chelators showed therapeutic potential in preferentially targeting CALR(de)(l52)-mutant cells, suggesting that modulation of intracellular zinc levels could be a novel approach to inhibit the oncogenic activity of CALR(de)(l52) in MPNs treatment.
Article
Cell Biology
Hyunsoo Rho, Alexander R. Terry, Constantinos Chronis, Nissim Hay
Summary: HK2 expression in activated HSCs is crucial for induced gene expression by histone lactylation. Inhibiting lactate production weakens HSC activation while exogenous lactate supplementation rescues it. Our findings suggest that HK2 may be a potential therapeutic target for liver fibrosis.
Article
Cell Biology
Shubha Murthy, Sankar Baruah, Jayden L. Bowen, Kathy Keck, Brett A. Wagner, Garry R. Buettner, David B. Sykes, Julia Klesney-Tait
Summary: Inflammatory agents, microbial products, or stromal factors can pre-activate neutrophils to respond rapidly and aggressively to subsequent stimuli, a process known as priming. Primed neutrophils exhibit enhanced chemotaxis, phagocytosis, and respiratory burst. TREM-1 plays a critical role in primed phagocytic respiratory burst in neutrophils.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Cell Biology
Tara R. Richman, Judith A. Ermer, Jessica Baker, Stefan J. Sira, Benjamin T. Kile, Matthew D. Linden, Oliver Rackham, Aleksandra Filipovska
Summary: The gene expression of mitochondria in platelets is essential for their function and regulation of blood clot formation. Conditional deletion of Elac2, Ptcd1, or Mtif3 genes in platelets leads to increased megakaryocyte ploidy, thrombocytopenia, and extended bleeding time. Mitochondrial gene expression in platelets is necessary for normal hemostasis, blood coagulation, and fibrinolysis.
Article
Hematology
Katrijn R. Six, Caitlin Debaene, Michelle Van den Hauwe, Riet De Rycke, Elizabeth E. Gardiner, Veerle Compernolle, Hendrik B. Feys
Summary: This study aimed to determine the spatiotemporal control over GPIba shedding by ADAM17 and found that strong and sustained stimulation induces the shedding, while mild activation does not. The study also revealed that proteinaceous ADAM17 inhibitors cannot inhibit shedding, but small molecule ADAM inhibitors can. This suggests the presence of two distinct GPIba subpopulations on platelets, with one involved in primary hemostasis and the other serving as a substrate for ADAM17 proteolysis with a potential role beyond hemostasis.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Cell Biology
Fengqi Duan, Cheng Zeng, Sijun Liu, Jianfeng Gong, Jia Hu, Hongyu Li, Hongmei Tan
Summary: The study confirmed that nicotine triggers NLRP3 inflammasome activation and induces macrophage migration into atherosclerotic plaque, thus accelerating atherosclerosis in apoE(-/-) mice fed with a high-fat diet. Nicotine increased α1-nAChR expression and stimulated its accumulation in lipid raft, leading to NLRP3 inflammasome activation in macrophage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Annie Qiu, Anabel Miller, Flavia Dei Zotti, Manjula Santhanakrishnan, Jeanne E. Hendrickson, Maria Tredicine, Sean R. Stowell, Chance John Luckey, James C. Zimring, Krystalyn E. Hudson
Summary: Passive immunization with anti-D can prevent maternal alloimmunization to RhD, but it may fail in some cases and even enhance alloimmunization. This study identified that FcγRIV on splenic conventional dendritic cells (cDCs) is required for IgG2c-mediated enhancement of RBC alloantibody production. FcγRI can cause RBC clearance without increasing alloimmunization. These findings have implications for the standardization of antibody subclasses in immunoprophylaxis preparations.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Andriy S. Yatsenko, Mariya M. Kucherenko, Yuanbin Xie, Henning Urlaub, Halyna R. Shcherbata
Summary: Studies have shown that dystroglycan has a spatiotemporally dynamic expression pattern in the brain during development, and proteins in the exocyst complex are associated with Dg in neurons. These findings help further understand the regulatory mechanisms of Dg in the brain.
Article
Cell Biology
Qinqin Peng, Ke Sha, Yingzi Liu, Mengting Chen, San Xu, Hongfu Xie, Zhili Deng, Ji Li
Summary: The study found that angiogenesis was enhanced in both rosacea patients and LL37-induced rosacea-like mice, with inhibition of angiogenesis alleviating rosacea-like features. Activation of mTORC1 in endothelial cells was observed in the lesional skin, and inhibiting mTORC1 decreased angiogenesis and blocked rosacea development. In vitro results showed that inhibiting mTORC1 signaling significantly decreased LL37-induced tube formation of human endothelial cells, suggesting that targeting angiogenesis may be a potential therapy for rosacea.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Hematology
Peter A. A. Norris, Gurleen Kaur, Ramsha Khan, Guangheng Zhu, Heyu Ni, Alan H. Lazarus
Summary: Anti-CD44 exhibits anti-inflammatory effects in murine ITP by inhibiting macrophage phagocytosis through blockade of Fc gamma R, potentially serving as an alternative to IVIg. The specific Fc gamma R blockade mechanism of anti-CD44 contributes to its therapeutic efficacy in ITP models, highlighting its potential as a treatment option.
Article
Hematology
Jia-feng Wang, Yun-peng Wang, Jian Xie, Zhen-zhen Zhao, Sahil Gupta, Yu Guo, Song-hui Jia, Jean Parodo, John C. Marshall, Xiao-ming Deng
Summary: The study reveals that increased PD-L1 expression delays apoptosis in neutrophils from septic patients, triggering PI3K-dependent AKT phosphorylation to drive lung injury and increase mortality.
Article
Public, Environmental & Occupational Health
Carl Otto Schell, Karima Khalid, Alexandra Wharton-Smith, Jacquie Oliwa, Hendry R. Sawe, Nobhojit Roy, Alex Sanga, John C. Marshall, Jamie Rylance, Claudia Hanson, Raphael K. Kayambankadzanja, Lee A. Wallis, Maria Jirwe, Tim Baker
Summary: The study has specified the content of care that should be provided to all critically ill patients, and proposed essential diagnosis-specific care for critically ill patients with COVID-19 in the ongoing pandemic, which could be an effective strategy for policy makers to reduce preventable deaths worldwide.
Article
Hematology
Pengcheng Xu, Yajing Zhao, Tianshu Yu, Yafei Yu, Xiaofei Ni, Haoyi Wang, Lu Sun, Panpan Han, Lingjun Wang, Tao Sun, Xinguang Liu, Hai Zhou, Jun Peng, Ming Hou, Yu Hou, Miao Xu
Summary: The study investigated the therapeutic effect of atorvastatin on immune thrombocytopenia (ITP). It was found that atorvastatin could inhibit the excessive activation of CD4(+) T cells and restore their function, potentially serving as a potential treatment option for ITP.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Hematology
Chaoyang Li, Meng Sun, Ranran Li, Shuwen Wang, Linlin Shao, Miao Xu, Meiyue Yao, Lin Wang, Ming Hou, Qi Feng, Jun Peng
Summary: This study retrospectively investigated 458 patients with ITP and found that 7.6% of them had comorbid T2DM. Patients with pre-existing T2DM had a better response to therapy, especially when metformin was included in the treatment regimen.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Hematology
Jing Qin, Qiang Liu, Anli Liu, Shaoqiu Leng, Shuwen Wang, Chaoyang Li, Ji Ma, Jun Peng, Miao Xu
Summary: The study found that in immune thrombocytopenia (ITP), the metabolism of CD4(+) T cells shifts from oxidative phosphorylation to glycolysis. Empagliflozin can affect the differentiation of CD4(+) T-cell subsets and regulate metabolic reprogramming in CD4(+) T cells. These findings suggest that empagliflozin may be a potential therapeutic option for ITP.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Cell Biology
Pedro Melo, Renato Socodato, Mariana S. Silveira, Miguel Antonio Dias Neves, Joao Bettencourt Relvas, Ines Mendes Pinto
Summary: Microglia, the primary immune cells in the CNS, play a crucial role in maintaining tissue homeostasis by changing their morphology and producing inflammatory mediators. Recent studies have highlighted the importance of actin and myosin II in controlling microglial cell shape.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Hematology
Xiaofei Ni, Lingjun Wang, Haoyi Wang, Tianshu Yu, Jie Xie, Guosheng Li, Yanfeng Liu, Hai Zhou, Miao Xu, Ming Hou, Jun Peng, Yu Hou
Summary: The metabolic fitness of Myeloid-derived suppressor cells (MDSCs) is crucial for their suppressive activity on effector T cells. Low-dose decitabine was found to promote the generation of MDSCs and enhance their aerobic metabolism and immunosuppressive functions. The study also revealed the impact of liver kinase 1 (LKB1) expression on MDSC function.
Article
Hematology
Hanna Wabnitz, Yoelys Cruz-Leal, Alan H. Lazarus
Summary: Despite antigen disparity between donor and recipient red blood cells, only a small percentage of transfusion patients develop alloantibody response. This study investigated the role of antigen density and cell dosage in primary and secondary immunization. Results suggest that both antigen copy number and cell dosage determine the outcome of immunization, and IgG subclass composition may serve as an indicator of RBC alloimmunization level and distinguish between primary and secondary immunization.
Editorial Material
Medicine, General & Internal
John C. Marshall, Aleksandra Leligdowicz
Summary: Infection leads to sepsis, and the development of clinical disease is triggered by the innate immune response of the host. However, the advancement of understanding in this biological response has not been matched by successful treatment development. The COVID-19 pandemic has brought about changes in this aspect, showing potential therapeutic approaches and the possibility of evaluating them. This article reviews the history of mediator-targeted therapy for sepsis and discusses the conceptual, biological, technological, and organizational challenges that need to be addressed to develop effective treatments for a leading cause of global morbidity and mortality.
Article
Medicine, General & Internal
Yun Wang, Lei Sheng, Fengjiao Han, Qiuyu Guo, Zihan Zhang, Yu Hou, Qi Feng, Hai Zhou, Xuebin Ji, Jun Peng, Ming Hou, Miao Xu
Summary: This study compared the efficacy and safety of different treatments for newly diagnosed primary immune thrombocytopenia through a systematic review and network meta-analysis. The combined regimens showed better early and sustained responses compared to monotherapy. Dexamethasone plus recombinant human thrombopoietin ranked first for early response, while rituximab plus corticosteroids achieved the best sustained response. Additional treatments such as oseltamivir, all-trans retinoic acid, or tacrolimus with dexamethasone also achieved promising sustained responses without compromising safety. However, more head-to-head randomized controlled trials with larger sample sizes are needed for direct comparison among these strategies.
Editorial Material
Hematology
Alan H. Lazarus, John W. Semple
Summary: A study found that patients with chronic ITP have clonal expansions of a specific subset of CD8 T cells called terminally differentiated effector memory (TEMRA) T cells. These cells persist over the course of the disease, are more prominent in refractory ITP, and are more prevalent when the platelet count is low.
Article
Chemistry, Multidisciplinary
Miguel A. D. Neves, Sladjana Slavkovic, Yiming Wang, Yfke Pasman, Aron A. Shoara, Reid C. Gallant, Guangheng Zhu, Pasquale Benvenuto, Philip E. Johnson, Michael Thompson, Heyu Ni
Summary: This study develops a biomimetic platform for oriented immobilization of integrin heterodimers, which has broad impact in multiple fields.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Engineering, Electrical & Electronic
Haonan Zhao, Zhongying Xue, Xiaozhong Wu, Zhihuan Wei, Qiuyu Guo, Miao Xu, Chunyan Qu, Chunyu You, Yongfeng Mei, Miao Zhang, Zengfeng Di, Qinglei Guo
Summary: Transient electronics have significant importance in implantable bioelectronics due to their ability to disappear or degrade over a defined operational period. However, the dissolution of commonly used bioresorbable materials often leads to hydrogen production, which can potentially harm the human body. This paper introduces germanium nanomembrane-based bioresorbable electronic sensors that do not produce any gaseous products during their dissolution in biofluids. The superior electronic transport properties of germanium enable these sensors to successfully distinguish different physiological signals and show promise for the development of dual or multi-parameter biosensors.
NPJ FLEXIBLE ELECTRONICS
(2022)
Review
Biochemical Research Methods
Maria Vieira, Rosa Fernandes, Antonio F. Ambrosio, Vanessa Cardoso, Mariana Carvalho, Peng Weng Kung, Miguel Antonio Dias Neves, Ines Mendes Pinto
Summary: Diabetic retinopathy is the most common diabetic eye disease and the leading cause of vision loss in working-age adults worldwide. Current treatments are effective in advanced stages but have limited control over the progression of the disease in the early stages. Therefore, new technologies are needed for accurate and early diagnosis of DR.