Article
Biotechnology & Applied Microbiology
Tingting Long, Peng Zhang, Jingze Yu, Yushan Gao, Xiaoqin Ran, Yonghao Li
Summary: By adding miglitol to the fermentation medium of T. reesei, the cellulase titer increased more than two-fold. This method has potential in stimulating cellulase production and provides a promising system for T. reesei protein expression.
FERMENTATION-BASEL
(2022)
Article
Microbiology
Ai-Ping Pang, Haiyan Wang, Yongsheng Luo, Zihuayuan Yang, Zhiyu Liu, Zhao Wang, Bingzhi Li, Song Yang, Zhihua Zhou, Xiaolin Lu, Fu-Gen Wu, Zuhong Lu, Fengming Lin
Summary: This study revealed that overexpression of cel3b or cel3g significantly increased extracellular beta-glucosidase production, while overexpression of cel1b inhibited cellulase production. Four cellular distribution patterns of beta-glucosidases in T. reesei were identified, providing insight into their role in cellulase production and cell function. Furthermore, the study elucidated the secretion pathways of different beta-glucosidases, advancing understanding of their properties in filamentous fungi.
Article
Chemistry, Multidisciplinary
Peng Zhang, Qian Li, Yudian Chen, Nian Peng, Wenshu Liu, Xuemei Wang, Yonghao Li
Summary: This study developed a method using the byproduct of sophorose from stevioside acid hydrolysis as an inducer for cellulase production, and found that it induced cellulase production more effectively than other common soluble inducers. The crude enzyme using MGS as an inducer showed good performance in hydrolyzing corn stover. This research has the potential to reduce the production cost of inducer and contribute to improving cellulase production in industrial-scale.
Article
Biotechnology & Applied Microbiology
Qinqin Zhao, Zezheng Yang, Ziyang Xiao, Zheng Zhang, Jing Xing, Huiqi Liang, Liwei Gao, Jian Zhao, Yinbo Qu, Guodong Liu
Summary: This study identified alanine 873 as a potential target for engineering XYR1 to improve lignocellulolytic enzyme production in Trichoderma reesei. Mutation of this residue to tyrosine significantly enhanced the production of xylanolytic enzymes, especially when glucose was used as the carbon source.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Su Yan, Yan Xu, Xiao-Wei Yu
Summary: The review summarizes recent progress in cellulase production of the filamentous fungus Trichoderma reesei, highlighting key factors such as carbon source-dependent induction, transcriptional regulation, and protein assembly. These findings shed light on potential directions for further engineering.
BIORESOURCES AND BIOPROCESSING
(2021)
Review
Biotechnology & Applied Microbiology
Hao Fang, Chaofeng Li, Jiajia Zhao, Chen Zhao
Summary: This review summarizes the biotechnological advances in engineering T. reesei for enhanced cellulase production and proposes innovative ideas for optimizing cellulase composition at the transcriptional level and improving cellulase production at the regulation level. Efficient genome editing and the developments of tools for multiple gene manipulations are essential for achieving these goals.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Yonghao Li, Peng Zhang, Deying Zhu, Bo Yao, Tomohisa Hasunuma, Akihiko Kondo, Xinqing Zhao
Summary: In this study, a recombinant T. reesei strain overexpressing the beta-glucosidase gene was used to achieve high cellobiase activity. It was found that a remarkably shorter transglycosylation time was sufficient under optimized conditions. The crude cellobiase from the recombinant strain was also used to blend with crude cellulase for high glucose yield in corn stover hydrolysis.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Agricultural Engineering
Gen Zou, Dapeng Bao, Ying Wang, Sichi Zhou, Meili Xiao, Zhanshan Yang, Yinmei Wang, Zhihua Zhou
Summary: Introducing product-activated cellulase genes significantly increases enzyme activity and saccharification efficiency. Improving thermostability of cellulases was achieved through mutants and enhanced enzyme activity. Introducing product-activated cellulase genes is an effective approach to alleviate product inhibition of cellulases.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Toshiharu Arai, Mayumi Wada, Hiroki Nishiguchi, Yasushi Takimura, Jun Ishii
Summary: The filamentous fungi Trichoderma reesei, commonly used for lignocellulosic biomass-degrading enzymes production, has not been widely utilized for heterologous recombinant protein production. This study demonstrates a novel inducer-free biomass-degrading enzyme expression system that allows the high secretory production of protein of interest (POI) in T. reesei using glucose as the sole carbon source. By replacing major cellulase genes with genes encoding enzymes and nanobodies, the occupancy of recombinant proteins in the secreted proteins was significantly increased.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Yudian Chen, Yushan Gao, Zancheng Wang, Nian Peng, Xiaoqin Ran, Tingting Chen, Lulu Liu, Yonghao Li
Summary: The knockout of the Trctf1 gene does not significantly affect cellulase synthesis induced by soluble inducers, but it increases the activities of cellulase, cellobiohydrolase, and endoglucanase by 20.2%, 12.4%, and 12.9% respectively when induced with lactose. The deletion of Trctf1 has little effect on the cellulase cocktail and the hydrolysis efficiency on corn stover. These findings provide insights into the regulation of cellulase synthesis by different soluble inducers and the construction of high-yield cellulase strains.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Su Yan, Yan Xu, Xiu-Mei Tao, Xiao-Wei Yu
Summary: It was found that knocking out vps10, vps13, and vps21 genes in T. reesei improved cellulase production by alleviating vacuolar transport and degradation. Transcriptome analysis indicated that increased cellulase gene expression might be attributed to the differential expression of sugar transporters. This study provides new clues for higher cellulase production in T. reesei through the alleviation of vacuolar transport via knockout of vps10, vps13, and vps21.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Jing Li, Yumeng Chen, Ao Gao, Liujing Wei, Dongzhi Wei, Wei Wang
Summary: A T. reesei cell factory was constructed for the first time to produce ig-carotene. By overexpressing key enzymes including CarRP, CarB, GGS1/CrtE, and HMG1, and optimizing the concentration of medium components, the modified strain achieved a titer of 218.8 mg/L of ig-carotene in flask culture. This study offers a novel and unique approach to simultaneously produce proteins and carotenoids using T. reesei industrial strains, addressing the costly waste mycelium management process.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Yanli Cao, Renfei Yang, Fanglin Zheng, Xiangfeng Meng, Weixin Zhang, Weifeng Liu
Summary: The ISW1 gene in Trichoderma reesei plays a crucial role in regulating cellulase and yellow pigment biosynthesis, with its deletion affecting growth and spore formation while enhancing pigment production.
Article
Microbiology
Yanli Cao, Renfei Yang, Fanglin Zheng, Xiangfeng Meng, Weixin Zhang, Weifeng Liu
Summary: This study identified a chromatin remodeling factor, TrISW1, with a dual role in coordinating the cellulolytic response and biosynthesis of the major secondary metabolite in T. reesei.
Review
Biotechnology & Applied Microbiology
Shailja Pant, Piyali Ritika, Piyali Nag, Amit Ghati, Dipjyoti Chakraborty, Mariana Rocha Maximiano, Octavio Luiz Franco, Amit Kumar Mandal, Arindam Kuila
Summary: This article mainly introduces the application of CRISPR/Cas9 technology in cellulase engineering in T. reesei. The emergence of new genetic tools over the past decade has strengthened the understanding of the transcription mechanism of cellulase genes in fungi and provided a method for editing them at the molecular level. However, the application of CRISPR technology in fungi still faces challenges and limitations.
BIOTECHNOLOGY ADVANCES
(2022)
Review
Biochemistry & Molecular Biology
Shunqin Zhu, Qin Yu, Chunsong Huo, Yuanpeng Li, Linshen He, Botian Ran, Ji Chen, Yonghao Li, Wanhong Liu
Summary: Artemisinin and its derivatives are potential anticancer drugs that induce cancer cell apoptosis, promote cell cycle arrest and autophagy, and inhibit cancer cell invasion and migration. They also increase intracellular Reactive Oxygen Species (ROS) levels, leading to ferroptosis, a new form of cell death considered as a cancer suppressor and a novel mechanism for cancer therapy.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Ophthalmology
Xiujuan Zhao, Yonghao Li, Wei Ma, Ping Lian, Xiling Yu, Shida Chen, Xia Huang, Bingqian Liu, Lin Lu
Summary: This study compared the efficacy of MB and PPV for FTMH and MD in highly myopic eyes. Both treatments improved BCVA significantly, but MB had a higher success rate in retinal reattachment and lower surgical failure rate compared to PPV. Myopic maculopathy development may be more severe following PPV surgery.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Ophthalmology
Jun Li, Xiujuan Zhao, Shida Chen, Bingqian Liu, Yonghao Li, Ping Lian, Yanbing Wang, Xiling Yu, Lin Lu
Summary: The patterns of fundus autofluorescence in patients with different grades of myopic atrophy maculopathy were investigated. The severity of FAF was significantly correlated with myopic characteristics and FAF could be beneficial for detecting unremarkable patchy chorioretinal atrophy on color fundus photographs of MAM.
CURRENT EYE RESEARCH
(2021)
Article
Ophthalmology
Jiaqing Zhang, Wei Wang, Zhenzhen Liu, Guangyao Yang, Xiaozhang Qiu, Jingmin Xu, Guangming Jin, Yonghao Li, Shaochong Zhang, Xuhua Tan, Lixia Luo, Yizhi Liu
Summary: This study compared the performance of new-generation and traditional intraocular lens calculation formulas in eye surgeries, with new-generation formulas showing better accuracy, especially the EVO formula. In addition, for eyes filled with silicone oil, the WK adjustment can effectively improve prediction accuracy.
JOURNAL OF CATARACT AND REFRACTIVE SURGERY
(2021)
Article
Ophthalmology
Jun Li, Bingqian Liu, Yonghao Li, Xiujuan Zhao, Shida Chen, Xia Huang, Ping Lian, Tao Li, Chenjin Jin, Xiaoling Liang, Lin Lu
Summary: The study analyzed clinical characteristics in eyes with myopic traction maculopathy (MTM) and found that with the severity of MTM, age increased and best-corrected visual acuity became worse, while spherical equivalent refraction and axial length were not correlated with the severity of the disease. Significant differences were found between eyes with inner foveoschisis and outer foveoschisis, and between full-thickness macular hole with and without macular schisis, indicating potential differences in pathogenesis.
RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES
(2021)
Article
Ophthalmology
Jun Li, Yonghao Li, Shida Chen, Wei Ma, Xiujuan Zhao, Xia Huang, Ping Lian, Bingqian Liu, Lin Lu
Summary: The study found that patients with intact ellipsoid zone (EZ) after macular buckling surgery showed better visual acuity and subfoveal fluid (SFF) disappearance compared to those with disrupted EZ.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yonghao Li, Peng Zhang, Deying Zhu, Bo Yao, Tomohisa Hasunuma, Akihiko Kondo, Xinqing Zhao
Summary: In this study, a recombinant T. reesei strain overexpressing the beta-glucosidase gene was used to achieve high cellobiase activity. It was found that a remarkably shorter transglycosylation time was sufficient under optimized conditions. The crude cellobiase from the recombinant strain was also used to blend with crude cellulase for high glucose yield in corn stover hydrolysis.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemical Research Methods
Jianghong Li, Yudian Chen, Yushan Gao, Yi Mo, Tingting Long, Bo Yao, Yonghao Li
Summary: It was found that overexpressing the endogenous Trcip1 gene can enhance the cellulase production capability of Trichoderma reesei Rut C30, leading to improved hydrolysis efficiency of corn stover.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Engineering, Chemical
Yudian Chen, Nian Peng, Yushan Gao, Qian Li, Zancheng Wang, Bo Yao, Yonghao Li
Summary: This study presents a novel two-stage pretreatment process design for recycling black liquor in the bioconversion of Jerusalem artichoke stalk. Alkali treatment was used to recover lignin from the black liquor, which was subsequently used for the second time to dissolve hemicellulose. The pretreatment strategy using alkali and acid combined with wastewater recycling provides an alternative approach for cellulosic biorefinery.
Article
Thermodynamics
Yonghao Li, Zhaolin Chen, Yi Feng, Meinan Liu, Chuanzhi Kang, Kaijie Yang, Jie Yuan, Chenghui Qiu, Hong Shi, Yanlong Jiang
Summary: This study proposes a novel petal-type battery thermal management system (BTMS) with dual phase change materials (PCM) that significantly enhances the environmental adaptability of the battery. Numerical simulations were used to investigate the thermal performance of the BTMS under different ambient temperatures and discharge rates. The results show that the proposed BTMS exhibits the best thermal performance and environmental adaptability compared to other conventional BTMS under typical ambient temperatures. Additionally, the BTMS was optimized using fin-reinforced heat transfer technology, resulting in reduced maximum temperature difference (ATmax) and maximum temperature rise (ATmrise) at ambient temperatures of 30°C and 40°C.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2023)
Article
Biotechnology & Applied Microbiology
Yudian Chen, Yushan Gao, Zancheng Wang, Nian Peng, Xiaoqin Ran, Tingting Chen, Lulu Liu, Yonghao Li
Summary: The knockout of the Trctf1 gene does not significantly affect cellulase synthesis induced by soluble inducers, but it increases the activities of cellulase, cellobiohydrolase, and endoglucanase by 20.2%, 12.4%, and 12.9% respectively when induced with lactose. The deletion of Trctf1 has little effect on the cellulase cocktail and the hydrolysis efficiency on corn stover. These findings provide insights into the regulation of cellulase synthesis by different soluble inducers and the construction of high-yield cellulase strains.
FERMENTATION-BASEL
(2023)
Article
Chemistry, Multidisciplinary
Peiyuan Wang, Yihang Hou, Gencheng Deng, Zhuofan Liu, Yonghao Li, Denggui Zhu, Dongjie Guo, Sunmin Sun
Summary: Pomegranate-like FeSe2@NC microspheres were successfully fabricated through a hydrothermal method and subsequent annealing treatment. These microspheres exhibited superior electrochemical performance as an anode material for sodium-ion batteries, with high specific capacity, superior rate performance, and long cycling stability. The excellent electrochemical performance suggests that FeSe2@NC microspheres can be considered a promising high-rate anode for sodium-ion batteries.
Article
Chemistry, Multidisciplinary
Peng Zhang, Qian Li, Yudian Chen, Nian Peng, Wenshu Liu, Xuemei Wang, Yonghao Li
Summary: This study developed a method using the byproduct of sophorose from stevioside acid hydrolysis as an inducer for cellulase production, and found that it induced cellulase production more effectively than other common soluble inducers. The crude enzyme using MGS as an inducer showed good performance in hydrolyzing corn stover. This research has the potential to reduce the production cost of inducer and contribute to improving cellulase production in industrial-scale.
Article
Biotechnology & Applied Microbiology
Tingting Long, Peng Zhang, Jingze Yu, Yushan Gao, Xiaoqin Ran, Yonghao Li
Summary: By adding miglitol to the fermentation medium of T. reesei, the cellulase titer increased more than two-fold. This method has potential in stimulating cellulase production and provides a promising system for T. reesei protein expression.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Yonghao Li, Jingze Yu, Peng Zhang, Tingting Long, Yi Mo, Jianghong Li, Qian Li
Summary: This study demonstrated that the mixture of glucose and beta-disaccharide (MGD) can efficiently induce cellulase production in Trichoderma reesei, providing a theoretical basis for understanding the molecular mechanisms involved. Transcriptomic analyses revealed that MGD elicits higher transcription of cellulases compared to lactose induction, while downregulating xylanases. Regulatory factors such as transcription activators and protein processing pathways are crucial for efficient cellulase production in T. reesei.
BIORESOURCES AND BIOPROCESSING
(2021)