期刊
BIOSENSORS & BIOELECTRONICS
卷 106, 期 -, 页码 99-104出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.01.061
关键词
Optical microfiber coupler; Biosensor; Dispersion turning point; Cardiac troponin I; Ultrasensitivity; Regeneration
类别
资金
- China-Israel International Science and Technology Cooperation Program [2015DFG02620]
- Natural Science Foundation of China [61504143, 51505456, 61727813]
- National High Technology Development Program of China [2015AA042402]
- Technology Development Program of Jilin province [20170414015GH, 20160520097JH]
Sensitive detection of cardiac biomarkers is critical for clinical diagnostics of myocardial infarction (MI) while such detection is quite challenging due to the ultra-low concentration of cardiac biomarkers. In this work, a label-free immunosensor based on optical microfiber coupler (OMC) has been developed for the ultrasensitive detection of cardiac troponin I (cTnI), a selective and highly sensitive biomarker of acute myocardial infarction (AMI). CTnI monoclonal antibodies were immobilized on the surface of the fiber through polyelectrolyte layer using layer-by-layer deposition technique. For refractive index sensing characterization, an ultra-high sensitivity of 91777.9 nm/RIU was achieved when the OMC works around the dispersion turning point, which is the highest experimental demonstration in the field of fiber-optic evanescent biosensors. For biosensing, the immunosensor with good specificity showed a linear wavelength shift in the range of 2-10 fg/mL and an ultra-low detection limit of 2 fg/mL. Such immunosensors have huge application potential for the detection of cardiac biomarkers of myocardial infarction due to simple detection scheme, quick response time, ease of handling and miniaturation.
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