Article
Chemistry, Analytical
Jianhui Xu, Ruijin Zeng, Lingting Huang, Zhenli Qiu, Dianping Tang
Summary: A magnetic-assisted platform was established for monitoring prostate-specific antigen (PSA), based on Bi2MoO6 nanosheets (BMO) and coupling the aptamer-guided hybridization chain reaction (HCR) with the hydrolysate-induced vulcanization reaction. The platform showed high precision with a quantitative diagnosis sensitivity area in a range of 5.0 pg mL(-1)-100 ng mL(-1).
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Biomedical
Chenguang Wang, Yi Xu, Shuainan Li, Yi Zhou, Qiuling Qian, Yifan Liu, Xianqiang Mi
Summary: Circulating tumor cells (CTCs) are biomarkers widely used for the early detection, diagnosis, and therapy monitoring of cancer. However, due to their rarity in peripheral blood, isolating and analyzing CTCs with high efficiency, purity, and viability has been a significant technological challenge. In this study, the researchers developed a microfluidic system combining tetrahedral DNA framework, herringbone channel chip, and aptamer-triggered hybridization chain reaction for efficient capture and release of simulated CTCs.
MATERIALS TODAY BIO
(2022)
Article
Chemistry, Analytical
Chuyan Zhang, Zewei Luo, Mengfan Wu, Wei Ning, Ziyi Tian, Yixiang Duan, Yongxin Li
Summary: A novel biosensor combining HCR and 3WJ-NEASA was developed for sensitive detection of food-borne bacteria, demonstrating broad application prospects in the field of food safety and environmental monitoring.
Article
Chemistry, Multidisciplinary
Bowen Zhang, Taoran Tian, Dexuan Xiao, Shaojingya Gao, Xiaoxiao Cai, Yunfeng Lin
Summary: A DNA-enhanced HCR detection system (T-probe system) is introduced for the detection of cancer-related targets, demonstrating its versatility and efficiency by detecting miRNA-21 and nucleolin. The T-probe system shows high detection rate and rapid recognition of both membrane and intracellular cancer-related targets, and exhibits superior fluorescence for imaging tumor-related biomarkers in vitro and in vivo, indicating its great potential for clinical diagnosis and therapeutic applications.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Dayong Li, Wenting Cheng, Zhiqiang Hou, Chengjie Duan, Yanheng Yao, Yan Chen, Gang Yang, Zhouxiang Cheng, Yang Xiang
Summary: In this study, a simple method using a functional RNA/DNA circuit is proposed for the detection of SARS-CoV-2 RNA. By integrating nucleic acid circuit technology and CRISPR/cas12a system, this method can achieve femtomolar detection of the target RNA in one step and successfully distinguish COVID-19 positive cases from clinical samples, demonstrating its great potential for clinical application.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biophysics
Shah Zada, Huiting Lu, Wenhao Dai, Songsong Tang, Sikandar Khan, Fan Yang, Yuchun Qiao, Pengcheng Fu, Haifeng Dong, Xueji Zhang
Summary: The study developed an amplified imaging platform for intracellular miRNAs based on Mo2B nanosheets, which combined fluorescence quenching and hybridization chain reaction technologies for sensitive detection and imaging of miRNAs. This platform demonstrated excellent performance in distinguishing cancer cells from normal cells and monitoring miRNA expression changes in cancer cells.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Cheng Fang, Yuming Li, Song Hu, Hao Wang, Xiaoxia Chen, Xiaoli Zhu
Summary: The developed DNA tree in this study enables amplified analysis of mRNA in living cells, achieving sensitive, accurate, and nondestructive analysis. The probes are transported into cells by exosomes, are LDNAs resistant to degradation, laying the foundation for reliable analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Mengyan Li, Nian Luo, Xiaofei Liao, Li Zou
Summary: We developed a dual signal amplification strategy for sensitive detection of circulating tumor DNA (ctDNA) based on hybridization chain reaction (HCR) and proximity hybridization-regulated CRISPR/Cas12a. This strategy exhibits high sensitivity toward ctDNA with a low detection limit of 5.43 fM, and it has been successfully applied for ctDNA detection in serum samples, showing great potential in the clinical diagnosis of cancer.
Article
Biochemistry & Molecular Biology
Mei Yang, Ruihua Ji, Zhengqing Zhao, Wenwen Wang, Ye Lu, Zhenghua Xiang, Hongbin Yuan
Summary: This study established a new method called In Situ Hybridization Chain Reaction (isHCR) for in situ detection of apoptotic DNA fragments. Compared to the current TUNEL method, isHCR can specifically detect DNA fragmentation in the cytoplasm of early-stage apoptotic cells, with low cost and the ability to handle large numbers of specimens. This suggests that isHCR has the potential to play a role in the in situ detection of DNA fragmentation in apoptotic cells.
Article
Chemistry, Physical
Shaofei Li, Yizhuang Cheng, Miao Qin, Guoliang Zhou, Pan Li, Liangbao Yang
Summary: In this study, two conceptual models of intelligent and robust DNA robots are proposed to perform leakless nonlinear signal amplification in nanotechnology and nanomedicine. These models are based on super-hairpin nanostructures, designed with innovative principles and codified into embedded programs. Dynamic and thermodynamic analyses reveal the critical elements and leak-resistant mechanisms of the models, which are then validated experimentally. The applications of the models in specific signal amplification and targeted payload enrichment are demonstrated.
NANOSCALE HORIZONS
(2022)
Article
Chemistry, Multidisciplinary
Yujia Zhang, Yue Yu, Ke Kang, Xuekang Wang, Nanhang Zhu, Xingyou Wang, Qiangying Yi, Yao Wu
Summary: A new nano-magnetic aptamer sensor platform has been developed for capturing various epithelial circulating tumor cells (CTCs) and simultaneously identifying their multiple organ origin markers. The platform has excellent capture and enrichment capabilities, and can trace metastatic cancer to related organs. This platform provides a feasible strategy for the accurate detection of multi-marker CTCs and a promising auxiliary tool for precise cancer treatment.
Article
Nanoscience & Nanotechnology
Qian Wang, Zuhao Zhang, Lu Zhang, Yunqing Liu, Li Xie, Shenguang Ge, Jinghua Yu
Summary: In this work, a CRISPR/Cas12a initiated switchable ternary electrochemiluminescence (ECL) biosensor combined with a Co3O4@Au nanoemitter is presented for the in vitro monitoring of miRNA-141. The biosensor demonstrated extraordinary sensitivity and specificity, with a low detection limit.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Iene Rutten, Devin Daems, Karen Leirs, Jeroen Lammertyn
Summary: Evalution(TM) is a novel microfluidic platform that combines encoded microparticles and channel-based microfluidics to enable fast, sensitive, and simultaneous detection of multiple disease-related biomarkers.
Article
Chemistry, Analytical
Hongyan Zhang, Siying Ye, Lishan Huang, Shen Fan, Weiwei Mao, Yijin Hu, Yuyan Yu, Fengfu Fu
Summary: A signal-on electrochemical aptasensor based on aptamer and hybridization chain reaction (HCR) amplification was developed for ultrasensitive detection of AFB1. The aptasensor achieved a linear detection range of 0.01-100 pg mL(-1) and a LOD down to 2.84 fg mL(-1). It was successfully applied to detect AFB1 in corn and traditional Chinese medicine samples, showing its potential value for practical AFB1 detection.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Multidisciplinary
Kunihiko Morihiro, Hiraki Osumi, Shunto Morita, Takara Hattori, Manami Baba, Naoki Harada, Riuko Ohashi, Akimitsu Okamoto
Summary: Artificial nucleic acids have been widely studied as potential cancer immunotherapeutic materials due to their ability to stimulate innate immune responses. However, their low selectivity for cancer cells limits their effectiveness. In this study, a hairpin DNA assembly technology is introduced to selectively activate immune responses in cancer cells that overexpress miR-21, leading to cytotoxicity. This approach provides a novel therapeutic modality for cancer treatment through selective intracellular DNA self-assembly.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)