Article
Biophysics
Patrycja Lach, Alvaro Garcia-Cruz, Francesco Canfarotta, Alistair Groves, Jakub Kalecki, Dominik Korol, Pawel Borowicz, Kostiantyn Nikiforow, Maciej Cieplak, Wlodzimierz Kutner, Sergey A. Piletsky, Piyush Sindhu Sharma
Summary: Redox-active MIP-Gly NPs were developed by incorporating ferrocenylmethyl methacrylate into the synthesis process, enabling them to selectively determine glyphosate without the need for redox probe. MIP-Gly NPs synthesized using allylamine and squaramide-based monomers exhibited the highest selectivity. The MIP-Gly NPs modified Pt-SPEs were characterized using differential pulse voltammetry and electrochemical impedance spectroscopy, with changes in the oxidation peak of the ferrocenyl moieties serving as the analytical signal. The chemosensor showed a low limit of detection and a wide linear dynamic concentration range for glyphosate, allowing successful detection in spiked river water samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Physical
Najeh Jaoued-Grayaa, Chaima Nasraoui, Yves Chevalier, Souhaira Hbaieb
Summary: The design of molecularly imprinted polymers (MIPs) for specific binding of hydrophobic molecules was investigated in this study. The results showed that the most hydrophobic monomer provided the highest selective adsorption, but the non-selective adsorption and softness of poly(lauryl methacrylate) had a negative impact on selectivity. The adsorption process was exothermic and had fast kinetics.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Engineering, Environmental
Ximin Chen, Long Jiang, Yaojie Liu, Lulu Zhang, Debin Yang, Xiaomeng Su, Zongjiang Yu, Mo Xian, Chao Xu
Summary: In this study, the optimal functional monomer VIM was quantitatively selected by DFT calculation, which exhibited the strongest affinity for PG among 11 phenolic monomers. The physicochemical property and morphology of the obtained VIM-MIP with the best adsorption performance were explored, and the adsorption mechanism was revealed to be driven by hydrogen bonding. The VIM-MIP showed a large adsorption capability for PG and excellent selectivity in binary systems.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Polymer Science
Keke Zhi, Zhe Li, Han Luo, Yitong Ding, Feiyan Chen, Yongxiang Tan, Hongrui Liu
Summary: In this study, a molecularly imprinted polymer (MIP) was synthesized using quercetin as the template and characterized. The MIP showed high selectivity and specific recognition towards quercetin, with an adsorption capacity of above 90% after five regeneration cycles, indicating potential application for selective adsorption of quercetin.
Article
Chemistry, Analytical
Pan Zhang, Gang Chen, Zhun Wang, Jiutong Ma, Qiong Jia
Summary: A sensor based on magnetic molecularly imprinted polymers (MMIPs) and surface-enhanced Raman scattering (SERS) was developed for the sensitive detection of transferrin (TRF). The sensor exhibited excellent specificity and sensitivity, and demonstrated reliability and feasibility for TRF detection in real serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Abderrahman Lamaoui, Jose Maria Palacios-Santander, Aziz Amine, Laura Cubillana-Aguilera
Summary: The study re-examined MIPs based on PDA, focusing on the effect of template removal on non-specific adsorption. Results indicated that the high difference between MIPs and NIPs based on PDA is not due to imprinted cavities but to the removal step applied to MIPs.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Gulgun Aylaz, Okan Zenger, Gozde Baydemir Pesint, Muge Andac
Summary: The HTr-MIPCC composite cryogel discs, synthesized with surface imprinting technique, exhibit high adsorption capacity and specificity for HTr, making them suitable for selective removal of HTr. Characterized by various methods, such as FTIR Spectrometer and SEM, the discs show increased surface area and ability for multiple reuse.
SEPARATION SCIENCE AND TECHNOLOGY
(2022)
Article
Polymer Science
Chaima Nasraoui, Najeh Jaoued-Grayaa, Laurent Vanoye, Yves Chevalier, Souhaira Hbaieb
Summary: This study addresses the possible manufacture of Molecularly Imprinted Polymers (MIPs) under the conditions of weak interaction between the functional monomer and template molecule. By preparing MIPs using different types of monomers and evaluating their performance, it was found that all MIPs showed similar adsorbent performance.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Nanoscience & Nanotechnology
Biao Wang, Yi Kuang, Minghui Li, Xing Wang, Xiaotian Zhang, Qingqing Rao, Bingnan Yuan, Shengxiang Yang
Summary: In this study, a magnetic surface molecularly imprinted polymer (Daidzein/SMIPs) was synthesized using magnetic surface molecular imprinting technology, which exhibited high adsorption capacity and exceptional selectivity for daidzein, enabling efficient separation in practical applications.
ADVANCED COMPOSITES AND HYBRID MATERIALS
(2023)
Article
Engineering, Electrical & Electronic
Lishuang Wang, Hailong Wang, Ximing Tang, Longshan Zhao
Summary: This study proposed a synthesized strategy for constructing a fluorescent biosensor for the detection of Lysozyme. The fluorescence intensity of the biosensor varied in the presence of Lysozyme due to electrostatic interactions on the surface. After optimization, the biosensor showed a high linear range and a low detection limit, and it was successfully applied to real samples.
SENSORS AND ACTUATORS A-PHYSICAL
(2022)
Article
Polymer Science
Pengpai Miao, Yanan Sang, Jie Gao, Xiaobing Han, Yuan Zhao, Tao Chen
Summary: Electron beam irradiation polymerization was used to fabricate L-Tyr molecularly imprinted polymers (MIPs) for selective separation of L-Tyr. The adsorption capacity of L-Tyr and the thermodynamic behavior were investigated by controlling the absorbed dosage and temperature. The obtained MIPs showed high adsorption capacity and selectivity.
Article
Chemistry, Analytical
Tiziano Di Giulio, Elisabetta Mazzotta, Cosimino Malitesta
Summary: In this study, electropolymerization of a scopoletin-based molecularly imprinted polymer for lysozyme detection was reported. Two different approaches were used for imprinting lysozyme, and the electrochemical responses of MIPs to Lyz were tested, showing good selectivity and competitive rebinding ability. The MIP prepared with enzyme immobilization exhibited higher imprinting factor and lower detection limit compared to the MIP prepared without enzyme immobilization.
Article
Chemistry, Multidisciplinary
Golnaz Heidari, Fereshte Hassanzadeh Afruzi, Ehsan Nazarzadeh Zare
Summary: In this study, an antibacterial magnetic molecularly imprinted polymer (MMIP) composite was successfully prepared to remove fluoroquinolone antibiotics from water. The MMIP showed antibacterial activity against Escherichia coli and Staphylococcus aureus, and exhibited high adsorption capacity for ciprofloxacin. The MMIP could be reused multiple times without significant reduction in adsorption efficiency.
Review
Environmental Sciences
Diana Samantha Villarreal-Lucio, Karla Ximena Vargas-Berrones, Lorena Diaz de Leon-Martinez, Rogelio Flores-Ramiez
Summary: Molecular imprinting polymers (MIPs) are synthetic materials that selectively retain molecules or analytes. They are widely used as extraction materials due to their low cost, easy preparation, reversible adsorption and desorption, and stability. MIPs are considered as a promising approach for the quantification and monitoring of emerging pollutants.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Yi Kuang, Yunlong Xia, Xing Wang, Qingqing Rao, Shengxiang Yang
Summary: The magnetic surface molecularly imprinted polymer (HC/SMIPs) can efficiently and selectively adsorb and remove 4-hydroxycoumarin from biological samples, showing great potential for application.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Analytical
Soma Papp, Jozsef Kozma, Tom Lindfors, Robert E. Gyurcsanyi
Article
Chemistry, Analytical
Laszlo Simon, Zsofia Bognar, Robert E. Gyurcsanyi
Article
Biochemistry & Molecular Biology
Zoltan Janos Tolnai, Judit Andras, Zsuzsanna Szeitner, Krisztina Percze, Laszlo Ferenc Simon, Robert E. Gyurcsanyi, Tamas Meszaros
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Zsofia Bognar, Robert E. Gyurcsanyi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Electrochemistry
Jozsef Kozma, Soma Papp, Robert E. Gyurcsanyi
Summary: By introducing ferrocenefunctionalized multi-walled carbon nanotubes, it is possible to add redox properties to purely capacitive solid contacts, thereby aiding in further controlling the standard potential of SCISEs.
ELECTROCHEMISTRY COMMUNICATIONS
(2021)
Article
Electrochemistry
Giorgio Caserta, Xiaorong Zhang, Aysu Yarman, Eszter Supala, Ulla Wollenberger, Robert E. Gyurcsanyi, Ingo Zebger, Frieder W. Scheller
Summary: This study explores the preparation of peptide-imprinted polymer nanofilms for selective recognition of human adult hemoglobin, utilizing a hierarchical approach with superior affinity and providing key mechanistic insights towards the stability of the formed MIPs.
ELECTROCHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Marek Debosz, Jozsef Kozma, Radoslaw Porada, Marcin Wieczorek, Justyna Paluch, Robert E. Gyurcsanyi, Jan Migdalski, Pawel Koscielniak
Summary: Urinalysis is a simple and non-invasive method for diagnosing and monitoring various health disorders. By integrating sensors with 3D printed devices, cost-effectiveness can be ensured for widespread use in measuring multiple parameters.
Article
Chemistry, Analytical
Xiaorong Zhang, Giorgio Caserta, Aysu Yarman, Eszter Supala, Armel F. Tadjoung Waffo, Ulla Wollenberger, Robert E. Gyurcsanyi, Ingo Zebger, Frieder W. Scheller
Summary: The study utilized epitope imprinting polymers to recognize parent proteins, revealing non-specific binding between target peptides and parent proteins, as well as stronger non-specific binding of HbA to MIPs than target peptides.
Article
Chemistry, Multidisciplinary
Zsofia Bognar, Marien de Jonge, Robert E. Gyurcsanyi
Summary: In situ labelling and encapsulation of viruses with silver nanoparticles enables their detection and quantification at the single particle level. Electrochemical measurement of the current peaks from the silver-coated viruses on the electrode allows for the linear correlation between frequency and virus count.
Article
Chemistry, Multidisciplinary
Zsofia Bognar, Eszter Supala, Aysu Yarman, Xiaorong Zhang, Frank F. Bier, Frieder W. Scheller, Robert E. Gyurcsanyi
Summary: This study presents a versatile approach for generating epitope-imprinted polymer-based microarrays for protein recognition on surface plasmon resonance imaging (SPRi) chips, which can rapidly screen binding kinetics of various targets, including the SARS-CoV-2 spike protein. The highly affine molecularly imprinted polymers (MIPs) developed in this study showed lower nanomolar dissociation constants for RBD, exceeding natural affinity levels and even binding SARS-CoV-2 virus particles with higher affinity.
Article
Chemistry, Analytical
Jozsef Kozma, Soma Tapp, Robert E. Gyurcsanyi
Summary: In this study, a new class of solid-contact material based on the covalent functionalization of multiwalled carbon nanotubes with a chemically stable redox molecule was proposed. This material showed excellent reproducibility and stability for potassium ion measurements, with no sensitivity towards O-2, CO2, and light.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jozsef Kozmaa, Soma Papp, Robert E. Gyurcsanyia
Summary: Solid-contact ion-selective electrodes (SCISEs) are the best electrode embodiment for miniaturized, wearable and disposable sensors for ion/electrolyte measurements in body fluids. However, large scale manufacturing with reproducible calibration parameters is a challenge for SCISEs. The galvanostatic intermittent titration technique (GITT) is introduced to determine the optimal preadjustment potential.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Zsofia Bognar, Jozsef Kozma, Norbert Kovacs, Robert E. Gyurcsanyi
Summary: In order to overcome the lack of diversity in functional monomers for protein-selective molecularly imprinted polymers (MIPs), a new concept of conjugating an electropolymerizable monomer unit (umbelliferone) to an amino acid has been introduced. A novel aspartate-umbelliferone (Asp-UMB) conjugate has been synthesized as the first representative of this class of monomers and its suitability to generate highly affine MIPs for proteins by epitope imprinting has been proven. MIP microarrays on surface plasmon resonance imaging (SPRi) chips were used to optimize and assess the binding kinetics, resulting in highly affine MIPs with equilibrium dissociation constants in the lower picomolar range.
Editorial Material
Chemistry, Analytical
Eric Bakker, Philippe Buhlmann, J. Justin Gooding, Robert E. Gyurcsanyi, Ernoe Pretsch
Article
Chemistry, Multidisciplinary
Xiaorong Zhang, Armel T. Waffo, Aysu Yarman, Norbert Kovacs, Zsofia Bognar, Ulla Wollenberger, Ibrahim M. El-Sherbiny, Rabeay Y. A. Hassan, Frank F. Bier, Robert E. Gyurcsanyi, Ingo Zebger, Frieder W. Scheller
Summary: This study aims to gain a mechanistic understanding of the formation of epitope-imprinted polymer nanofilms. The affinity towards different peptides is mainly determined by a four-amino acid motif present in the template peptide. However, the parent proteins RBD and spike protein exhibit higher affinity on the nanofilm surface.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)