Article
Biotechnology & Applied Microbiology
Su Yan, Yan Xu, Xiu-Mei Tao, Xiao-Wei Yu
Summary: It was found that knocking out vps10, vps13, and vps21 genes in T. reesei improved cellulase production by alleviating vacuolar transport and degradation. Transcriptome analysis indicated that increased cellulase gene expression might be attributed to the differential expression of sugar transporters. This study provides new clues for higher cellulase production in T. reesei through the alleviation of vacuolar transport via knockout of vps10, vps13, and vps21.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Cheng Yao, Ningning Sun, Weihao Gao, Yu Sun, Jiaxin Zhang, Hong Liu, Yaohua Zhong
Summary: The vacuole plays a vital role in the cellulase secretion pathway in the major industrial fungus Trichoderma reesei. The overexpression of the spt1 gene significantly improves cellulase secretion, indicating that it can be a powerful target for increasing cellulase production in this fungus.
FERMENTATION-BASEL
(2023)
Article
Agricultural Engineering
Neha Srivastava, Rajeev Singh, Manish Srivastava, Asad Syed, Dan Bahadur Pal, Ali H. Bahkali, P. K. Mishra, Vijai Kumar Gupta
Summary: The study utilized mixed fungal consortia for solid state fermentation to enhance cellulase production, and improved the thermal and pH stability of cellulase with NiFe2O4 nanoparticles. The treated cellulase was used for enzymatic hydrolysis of biowaste to biofuels, resulting in significantly higher sugar yield and productivity compared to control.
BIORESOURCE TECHNOLOGY
(2022)
Review
Chemistry, Applied
Chaozhong Xu, Shanshan Tong, Liqun Sun, Xiaoli Gu
Summary: Cellulase-mediated lignocellulosic biorefinery is crucial for producing high-value biofuels and chemicals, and immobilization of cellulase enzymes has greatly improved the efficiency, stability, and reusability of the process. This comprehensive review analyzes the principles and various approaches of cellulase immobilization, including physical adsorption, covalent binding, entrapment, and cross-linking. It also explores different carrier materials and emerging techniques such as multi-enzyme co-immobilization and 3D printing carriers, while addressing obstacles like diffusion limitations and loss of cellulase activity.
CARBOHYDRATE POLYMERS
(2023)
Article
Agriculture, Multidisciplinary
Jing Li, Yumeng Chen, Ao Gao, Liujing Wei, Dongzhi Wei, Wei Wang
Summary: A T. reesei cell factory was constructed for the first time to produce ig-carotene. By overexpressing key enzymes including CarRP, CarB, GGS1/CrtE, and HMG1, and optimizing the concentration of medium components, the modified strain achieved a titer of 218.8 mg/L of ig-carotene in flask culture. This study offers a novel and unique approach to simultaneously produce proteins and carotenoids using T. reesei industrial strains, addressing the costly waste mycelium management process.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Shailja Pant, Piyali Ritika, Piyali Nag, Amit Ghati, Dipjyoti Chakraborty, Mariana Rocha Maximiano, Octavio Luiz Franco, Amit Kumar Mandal, Arindam Kuila
Summary: This article mainly introduces the application of CRISPR/Cas9 technology in cellulase engineering in T. reesei. The emergence of new genetic tools over the past decade has strengthened the understanding of the transcription mechanism of cellulase genes in fungi and provided a method for editing them at the molecular level. However, the application of CRISPR technology in fungi still faces challenges and limitations.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Chemistry, Multidisciplinary
Peng Zhang, Qian Li, Yudian Chen, Nian Peng, Wenshu Liu, Xuemei Wang, Yonghao Li
Summary: This study developed a method using the byproduct of sophorose from stevioside acid hydrolysis as an inducer for cellulase production, and found that it induced cellulase production more effectively than other common soluble inducers. The crude enzyme using MGS as an inducer showed good performance in hydrolyzing corn stover. This research has the potential to reduce the production cost of inducer and contribute to improving cellulase production in industrial-scale.
Article
Biotechnology & Applied Microbiology
Mukund G. Adsul, Pooja Dixit, Jitendra K. Saini, Ravi P. Gupta, Sankara Sri Venkata Ramakumar, Anshu S. Mathur
Summary: In this study, a mutant strain RC-23-1 of Trichoderma reesei RUT-C30 was obtained through mutagenesis. This mutant strain not only showed higher cellulase production but also lower viscosity during enzyme production. It has unique colony morphology and growth pattern, making it a promising candidate for large scale cellulase production.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Biotechnology & Applied Microbiology
Su Yan, Yan Xu, Xiao-Wei Yu
Summary: The review summarizes recent progress in cellulase production of the filamentous fungus Trichoderma reesei, highlighting key factors such as carbon source-dependent induction, transcriptional regulation, and protein assembly. These findings shed light on potential directions for further engineering.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Biotechnology & Applied Microbiology
Jessica C. Rohrbach, Jeremy S. Luterbacher
Summary: This study highlights the interaction between experimental conditions and physical characteristics of biomass particles in affecting digestibility, emphasizing the role of internal mass transfer as substrate size increases and enzyme loading decreases. Adjusting enzyme and biomass loading based on wood particle size and cellulose accessibility is crucial for maintaining a strong concentration gradient and optimizing enzyme diffusion.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Green & Sustainable Science & Technology
Fernando Roberto Paz-Cedeno, Lucas Ragnini Henares, Eddyn Gabriel Solorzano-Chavez, Mateus Scontri, Flavio Pereira Picheli, Ismael Ulises Miranda Roldan, Rubens Monti, Samuel Conceicao de Oliveira, Fernando Masarin
Summary: The study showed that pretreatment with sulfite-NaOH was the most efficient in removing lignin while keeping cellulose intact, and had the best response to enzymatic hydrolysis with high conversion rates.
Article
Microbiology
Cheng Yao, Mengjie Yan, Kehang Li, Weihao Gao, Xihai Li, Jiaxin Zhang, Hong Liu, Yaohua Zhong
Summary: Disrupting the ERAD pathway by knocking out hrd1, hrd3, and der1 genes resulted in impaired growth and altered cellulase secretion in T. reesei. The mutants exhibited high sensitivity to cell wall-disturbing agents and decreased heat tolerance. The secretion of cellobiohydrolase and beta-glucosidase was declined in the mutants compared to the parental strain.
Review
Biotechnology & Applied Microbiology
Hao Fang, Chaofeng Li, Jiajia Zhao, Chen Zhao
Summary: This review summarizes the biotechnological advances in engineering T. reesei for enhanced cellulase production and proposes innovative ideas for optimizing cellulase composition at the transcriptional level and improving cellulase production at the regulation level. Efficient genome editing and the developments of tools for multiple gene manipulations are essential for achieving these goals.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2021)
Review
Energy & Fuels
Nevena Ilic, Marija Milic, Suncica Beluhan, Suzana Dimitrijevic-Brankovic
Summary: Cellulases have attracted worldwide attention as they can degrade cellulose in lignocellulosic biomass and transform it into bioethanol. The enzymatic hydrolysis of cellulose by cellulases is a crucial step in biofuel production due to the complex structure of lignocellulose. The demand for cellulases is rapidly growing due to their unique ability to hydrolyze the recalcitrant nature of lignocellulosic biomass. Continuous efforts are being made to develop cellulase mixtures/cocktails with improved performance and isolate new microorganisms as potential producers of cellulases for biofuel production. This review provides insight into current research on improving microbial cellulase production and the outlook for the cellulase market in industrial bioethanol production.
Article
Biotechnology & Applied Microbiology
Nozomu Shibata, Hiroshi Kakeshita, Kazuaki Igarashi, Yasushi Takimura, Yosuke Shida, Wataru Ogasawara, Tohru Koda, Tomohisa Hasunuma, Akihiko Kondo
Summary: Trichoderma reesei alpha-tubulin plays a role in the transcription of cellulase and hemicellulase genes, as well as in carbon catabolite repression (CCR). Disruption of the alpha-tubulin gene in T. reesei overcomes CCR and enhances enzyme production, making it a promising approach for creating next-generation enzyme-producing strains of T. reesei.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)