Article
Agricultural Engineering
Hang Guo, Tao Li, Yongteng Zhao, Xuya Yu
Summary: Copper treatment significantly increased astaxanthin and lipid synthesis in Haematococcus pluvialis, leading to upregulation of biosynthesis genes and antioxidant enzyme-related genes, as well as a decrease in reactive oxygen species levels. Additionally, copper induced the activation of protein kinases, facilitating the production of astaxanthin and lipids in the algae.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Hailiang Xing, Yongteng Zhao, Tao Li, Benyong Han, Peng Zhao, Xuya Yu
Summary: The combination of melatonin and putrescine enhances the production of astaxanthin (Asta) and lipids in Haematococcus pluvialis, increasing endogenous putrescine content and reducing oxidative stress levels. This strategy also upregulates carotenogenic, lipogenic, and antioxidant enzyme genes in the microalga.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Hailiang Xing, Qingqing Li, Yongteng Zhao, Hui Gao, Linpin Li, Yong Zhang, Xuya Yu
Summary: This paper investigates the role of proline in the production of biomass and astaxanthin in stress-induced Haematococcus pluvialis. The results show that proline supplementation enhances the biomass, astaxanthin, and lipid contents, as well as regulates chlorophyll, carbohydrate, and protein accumulation. Proline also increases endogenous proline content, while reducing reactive oxygen species and malondialdehyde levels, thus alleviating oxidative stress and promoting astaxanthin biosynthesis. These findings suggest that proline is an effective strategy to improve astaxanthin productivity in Haematococcus pluvialis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Qingqing Li, Xinnan Zhang, Yongteng Zhao, Hui Gao, Linpin Li, Yong Zhang, Xuya Yu
Summary: In this study, exogenous myo-inositol (MI) was used to induce natural astaxanthin and biolipid accumulation in Haematococcus pluvialis. MI addition resulted in increased astaxanthin and lipid content, while also elevating ethylene (ETH) levels and activating antioxidant enzyme-associated gene expression. The ETH signal played a positive role in stimulating astaxanthin biosynthesis under MI induction.
BIORESOURCE TECHNOLOGY
(2022)
Review
Agricultural Engineering
Yuanyua Ren, Jinquan Deng, Junchao Huang, Zhaoming Wu, Lanbo Yi, Yuge Bi, Feng Chen
Summary: Astaxanthin, an important antioxidant and nutraceutical compound, is widely used in dietary supplements and cosmetics. The refinery of astaxanthin from Haematococcus pluvialis is a growing industrial sector, as it can accumulate astaxanthin up to 5% of dry weight. Further research on production optimization and joint-production with lipids could make algal-derived astaxanthin more accessible for various applications.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Hana-Nur Fitriana, Soo-Youn Lee, Sun-A Choi, Ji-Ye Lee, Bo-Lam Kim, Jin-Suk Lee, You-Kwan Oh
Summary: This study investigated the technical feasibility of inducing astaxanthin biosynthesis in H. pluvialis using electric stimulation in a two-chamber bioelectrochemical system. An optimal voltage of 4 V (anodic current: 30 mA) effectively stimulated astaxanthin biosynthesis, resulting in a 36.9% increase in astaxanthin content in treated cells compared to untreated cells. Proper regulation of bioelectrochemical parameters can improve astaxanthin production in H. pluvialis culture using electric treatment.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
Petras Venckus, Ieva Endriukaityte, Kotryna Cekuolyte, Renata Gudiukaite, Andrius Pakalniskis, Egle Lastauskiene
Summary: This study tested the effects of silver nanoparticles on Haematococcus pluvialis and found that high concentrations of AgNPs significantly reduced culture productivity in green-stage cultures, while the impact on red-stage cultures was smaller. The addition of AgNPs also led to changes in cell morphology, including deformations, disrupted cytokinesis, and induced sexual reproduction.
Article
Plant Sciences
Danqiong Huang, Wenfu Liu, Qunju Hu, Hui Li, Chaogang Wang
Summary: This study identified a member of HATs (HpGCN5) from the economically important microalgae Haematococcus pluvialis and evaluated its expression pattern and correlation with genes involved in stress responses and biosynthesis of astaxanthin and triacylglycerols. The results suggest that HpGCN5 may play a role in the regulation of astaxanthin biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Rachel Cray, Ira Levine
Summary: This study quantifies the relationship between astaxanthin and ROS in H. pluvialis, confirming the role of ROS as a modulator of carotenogenesis and astaxanthin as a protective mechanism against oxidative stress. The findings can be applied to optimize commercial astaxanthin production, reducing production time requirements by over 10%.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Gannoru Kankanamalage Sanuji Hasara Nishshanka, Vinoj Chamilka Liyanaarachchi, Pemaththu Hewa Viraj Nimarshana, Thilini Udayangani Ariyadasa, Jo-Shu Chang
Summary: The microalga Haematococcus pluvialis has high biotechnological potential for producing various valuable compounds. Biorefining, which integrates multiple production processes in a single facility, can maximize the utilization of feedstock and reduce resource consumption. This review discusses the potential of biorefining H. pluvialis to promote more sustainable and cleaner production technologies.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Agricultural Engineering
Yongteng Zhao, Hui-Ping Wang, Chunli Yu, Wei Ding, Benyong Han, Shuxiang Geng, Delu Ning, Ting Ma, Xuya Yu
Summary: The study revealed that melatonin treatment upregulated the biosynthesis of astaxanthin and lipids in Haematococcus pluvialis, affecting the accumulation process through the regulation of gene expression and metabolic pathways. The treatment may promote coproduction of astaxanthin and lipids by regulating the carbon-nitrogen balance and antioxidant system.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Thomas Roach, Nina Boeck, Nina Rittmeier, Erwann Arc, Ilse Kranner, Andreas Holzinger
Summary: The study reveals that the desiccation tolerance in Haematococcus pluvialis is related to the color of the cells, with red cells showing better tolerance. The mechanisms of desiccation tolerance include changes in cell ultra-structure and synthesis of astaxanthin-rich lipid bodies. The processes of acclimation to desiccation under low and high light conditions have overlapping components, such as accumulation of fatty acids, lipid bodies, and astaxanthin. Atmospheric exposure alone can trigger acclimation to desiccation, and photosynthesis powers this process, but high light stress and dehydration are not strictly necessary.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Jae Been Lee, Yun Hwan Park, Min Seo Jeon, Sok Kim, Yoon-E Choi
Summary: In this study, the researchers successfully increased the production of astaxanthin in Haematococcus pluvialis by combining polyethylenimine (PEI) with chitosan. The polyethylenimine concentration had an effect on astaxanthin accumulation, while the molecular weight of chitosan did not. The treatment also increased the reactive oxygen species content in cells, leading to the upregulation of enzymes involved in astaxanthin biosynthesis. The PEI-chitosan fiber treatment showed high recyclability and maintained over 90% astaxanthin production efficiency.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Meng Wang, Wenxin Xu, Jie Yu, Yingying Liu, Haotian Ma, Chunli Ji, Chunhui Zhang, Jinai Xue, Runzhi Li, Hongli Cui
Summary: The present study found that supplementation of astaxanthin (ATX) dose-dependently reduced body weight, hepatic oxidative stress, and improved hepatic steatosis in diet-induced obese mice. Furthermore, ATX modulated gut microbiota composition and alleviated gut microbiota dysbiosis caused by a high-fat diet.
FRONTIERS IN NUTRITION
(2022)
Review
Agricultural Engineering
Bolam Kim, Soo Youn Lee, Aditya Lakshmi Narasimhan, Sangui Kim, You-Kwan Oh
Summary: The green microalga Haematococcus pluvialis is a valuable source of astaxanthin, a powerful antioxidant widely used in various industries. The unique cyst-like cell with a rigid three-layered cell wall formed during astaxanthin accumulation presents challenges for extraction. Various cell disruption methods and downstream processes for sustainable development are also discussed in this review.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Yongteng Zhao, Hui-Ping Wang, Chunli Yu, Wei Ding, Benyong Han, Shuxiang Geng, Delu Ning, Ting Ma, Xuya Yu
Summary: The study revealed that melatonin treatment upregulated the biosynthesis of astaxanthin and lipids in Haematococcus pluvialis, affecting the accumulation process through the regulation of gene expression and metabolic pathways. The treatment may promote coproduction of astaxanthin and lipids by regulating the carbon-nitrogen balance and antioxidant system.
BIORESOURCE TECHNOLOGY
(2021)
Article
Microbiology
Zi-Xu Wang, Na Li, Jun-Wei Xu
Summary: Efficient expression of the Vitreoscilla hemoglobin gene in G. lucidum increased the production of exopolysaccharides and enhanced their antioxidant activity, without changing their monosaccharide composition or molecular weight distribution.
Article
Agricultural Engineering
Qingqing Li, Jinkun You, Tengsheng Qiao, Du-bo Zhong, Xuya Yu
Summary: A major challenge in astaxanthin industrialization is the low productivity and high production costs. This study established a two-stage cultivation strategy using NaCl to improve biomass and astaxanthin production by Haematococcus pluvialis. NaCl application significantly enhanced biomass and astaxanthin content, while salinity stress also increased lipids and GABA contents and provided insights into the biosynthesis mechanism of astaxanthin.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Hailiang Xing, Yongteng Zhao, Tao Li, Benyong Han, Peng Zhao, Xuya Yu
Summary: The combination of melatonin and putrescine enhances the production of astaxanthin (Asta) and lipids in Haematococcus pluvialis, increasing endogenous putrescine content and reducing oxidative stress levels. This strategy also upregulates carotenogenic, lipogenic, and antioxidant enzyme genes in the microalga.
BIORESOURCE TECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Yongteng Zhao, Tengsheng Qiao, Dan Gu, Liyan Zhu, Xuya Yu
Summary: The study utilized a joint strategy of salinity stress and chemical induction to improve lipid production; the results demonstrated that the combined application of GABA or CaCl2 and salt stress stimulated biolipid synthesis in microalgae; the quality of algal lipid-derived biodiesel met commercial standards.
Article
Environmental Sciences
Tengsheng Qiao, Dan Gu, Liyan Zhu, Yongteng Zhao, Du-bo Zhong, Xuya Yu
Summary: This study evaluated the feasibility of a joint strategy involving myo-inositol (MI) and salinity stress on lipid productivity of Monoraphidium sp. QLY-1 in molasses wastewater (MW). The results showed that the combined treatment significantly increased lipid productivity and enhanced nutrient removal from the MW. Furthermore, the application of MI upregulated lipogenic gene expressions and activated autophagy and ethylene signaling, thereby mitigating salinity-induced damage.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Correction
Biotechnology & Applied Microbiology
Wei Ding, Jun Peng, Yongteng Zhao, Peng Zhao, Jun-Wei Xu, Tao Li, Xuya Yu
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Correction
Biotechnology & Applied Microbiology
Wei Ding, Peng Zhao, Jun Peng, Yongteng Zhao, Jun-Wei Xu, Tao Li, Russel J. Reiter, Huixian Ma, Xuya Yu
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Chemistry, Applied
Li-Na Zhao, Yu-Biao Cao, Qin Luo, Yong-Liang Xu, Na Li, Cheng-Xiao Wang, Jun-Wei Xu
Summary: In this study, a novel approach to enhance the production and bioactivities of Ganoderma exopolysaccharides was explored. The PMM1 gene was cloned and overexpressed in Ganoderma for the first time, leading to a significant increase in exopolysaccharide production. The transformed strain also exhibited stronger regulation effects on macrophage.
CARBOHYDRATE POLYMERS
(2022)
Article
Microbiology
Qin Luo, Na Li, Jun-Wei Xu
Summary: This study demonstrates the regulatory role of the methyltransferase LaeA in ganoderic acid biosynthesis in the basidiomycete Ganoderma lingzhi. Deletion of laeA leads to reduced concentration of ganoderic acids and transcription levels of related genes, while constitutive overexpression of laeA results in increased concentration of ganoderic acids.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yong-Liang Xu, Huan Yuan, Na Li, Jian-Hui Xiao, Jun-Wei Xu
Summary: A β-1,3-glucan synthase gene was cloned and overexpressed in Ganoderma lingzhi, leading to an increase in intra-cellular polysaccharides content. Simultaneous overexpression of this gene and UDP-glucose pyrophosphorylase gene further enhanced the production of polysaccharides, with higher glucose content and improved antioxidative and anti-senescence activities.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biology
Shuzhen He, Ruixian Xu, Huashan Yi, Zhixin Chen, Congjie Chen, Qiang Li, Qinqin Han, Xueshan Xia, Yuzhu Song, Junwei Xu, Jinyang Zhang
Summary: Histidine tags are widely used in protein recombination technology due to their minimal impact on protein structure and solubility. The study presents a one-step ELISA method for detecting His-tagged recombinant proteins, which is simple, rapid, and accurate.
OPEN LIFE SCIENCES
(2022)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
