期刊
BIOMEDICINE & PHARMACOTHERAPY
卷 101, 期 -, 页码 137-144出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2018.02.069
关键词
Green chemistry; Silver nanoparticles (AgNPs); DNA interaction; Cytotoxicity; Cancer cell lines; BSA interaction
资金
- Fundacao para a Ciencia e a Tecnologia FCT/MEC [UID/QUI/00100/2013]
- UCIBIO [UID/Multi/04378/2013]
- ERDF under the PT2020 Partnership Agreement [POCI-01-0145-FEDER-007728]
- [SFRH/BPD/90883/2012]
- [SFRH/BPD/76451/2011]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/76451/2011] Funding Source: FCT
Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.
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