Article
Biochemistry & Molecular Biology
Marzena Pawul-Gruba, Tomasz Kiljanek, Anna Madejska, Jacek Osek
Summary: A method for determining eight biogenic amines in ripened cheeses was developed and validated using HPLC-DAD, showing high accuracy and precision.
Article
Biochemistry & Molecular Biology
Yun Ai, Yan Ni Sun, Li Liu, Fang Yuan Yao, Yan Zhang, Feng Yi Guo, Wen Jie Zhao, Jian Li Liu, Ning Zhang
Summary: This study successfully characterized biogenic amines in different parts of Lycium barbarum L. using HPLC, revealing four different types of amines. The bark had the highest histamine content, while the flower had the highest putrescine content. There were significant differences in biogenic amine content among different parts of Lycium barbarum L., which should be considered when further utilizing them.
Article
Biochemistry & Molecular Biology
Marianna Ntorkou, Eleni Tsanaktsidou, Konstantina Chachlioutaki, Dimitrios G. Fatouros, Catherine K. Markopoulou
Summary: Homotaurine shows promising potential as a drug for treating neurodegenerative diseases such as Alzheimer's. The study investigates the permeability of homotaurine in vitro and finds that it exhibits superior permeability through the simulated blood-brain barrier compared to memantine, suggesting potential for enhanced drug delivery strategies targeting neurological conditions.
Article
Food Science & Technology
Ayoub Kounnoun, Adnane Louajri, Francesco Cacciola, Aicha El Baaboua, Luigi Mondello, Hajar Bougtaib, Naoual Alahlah, Meriem Stitou, Mohamed El Maadoudi
Summary: An HPLC-FLD method without sample clean-up procedure was developed for quantifying histamine in fish and fishery products. The method demonstrated good linearity, specificity, precision, accuracy, and satisfactory recoveries within a certain concentration range. It was found to be simple, rapid, and reliable, effectively evaluated by various tests and sample analyses.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Ming-Hsun Shen, Rakesh K. Singh
Summary: This study aimed to simplify the conventional aflatoxin analysis method by utilizing the immunoaffinity column and normal-phase chromatography to detect aflatoxins B1, B2, G1, and G2. The optimized mobile phase enhanced fluorescence signal and improved sample cleanup method significantly increased recovery and repeatability. The proposed method outperformed the official method and met the US FDA's standards for examining human food, with a prolonged shelf-life of the mobile phase under room temperature.
Article
Biochemistry & Molecular Biology
Yuki Soma, Yoshihiro Izumi, Takehiko Shimohira, Masatomo Takahashi, Yuri Imado, Saki Tominaga, Kanako Tokito, Kosuke Hata, Shoji Shinadama, Mana Oshiro, Yoshihiro Hayakawa, Takeshi Bamba
Summary: The study presents a novel HPLC-based amino acid quantification method called iPDAQ, which transforms the derivatization process from an offline chamber process to an online in-needle process. This method improves the throughput and reduces the consumption of derivatization reagents, allowing for the continuous analysis of a large number of samples.
Article
Chemistry, Analytical
Eleni Tsanaktsidou, Lamprini Kanata, Sofia Almpani, Constantinos K. Zacharis, Catherine K. Markopoulou
Summary: A new HPLC-FLD method was developed using dansyl chloride for the determination of genotoxic impurities NDMA and NDEA in Lisinopril API and its final product. The method was optimized to achieve high sensitivity and reliable results, and showed linear calibration with satisfactory limit of detection. The proposed procedure is relatively easy, rapid, and suitable for routine analysis tests.
Article
Biochemistry & Molecular Biology
Dimitrios Tsikas, Bibiana Beckmann, Svetlana Baskal, Gorig Brunner
Summary: Gas chromatography-mass spectrometry (GC-MS) is a useful method for quantitative determination of polyamines spermidine (SPD), putrescine (PUT), and biogenic amine agmatine (AGM) in biological samples. However, GC-MS analysis of biogenic amine histamine (HA) is challenging and less efficient. This study investigated the derivatization of HA and the effects of toluene, ethyl acetate, and oven temperature program on HA analysis. The simultaneous quantitative analysis of HA, AGM, PUT, and SPD requires the use of ethyl acetate for extraction and injection into GC-MS apparatus, and a starting column temperature of 40°C.
Review
Chemistry, Analytical
Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: Glutathione, as the most abundant antioxidant in living organisms, plays a crucial role in cells due to its free thiol group. Its determination using liquid chromatography has been widely studied and proves significant in understanding the development of human diseases.
MICROCHEMICAL JOURNAL
(2023)
Article
Food Science & Technology
Yasutaka Shigemura, Yu Iwasaki, Yoshio Sato, Tomomi Kato, Takuya Seko, Kenji Ishihara
Summary: We investigated the absorption of high-purity balenine (Bal) prepared from opah muscle in mouse blood. Using HPLC with phenyl isothiocyanate pre-column derivatization, we successfully isolated imidazole peptides and their constituents. Our results showed that Bal and 3-methylhistidine (3-Me-His) were detected in mouse blood 1 hour after the administration of Bal derived from opah. The concentrations of Bal and 3-Me-His significantly increased in plasma, but were undetectable in control and carnosine (Car)-administrated mice. This study provides the first report on the absorption of food-derived Bal in mouse blood and serves as a pilot study for future clinical trials.
Article
Chemistry, Applied
Lili Zhang, Jian-lin Wu, Pan Xu, Sheng Guo, Tingting Zhou, Na Li
Summary: This study found that natto has a higher ACE inhibitory effect than soybean and identified 246 amino acid compounds, with 187 of them only detected in natto. Dipeptides with ACE inhibitory abilities were the most abundant class and positively correlated with the increased ACE inhibitory activity of natto. Dynamic profiling revealed that the increased dipeptides in natto were generated from protein via Bacillus subtilis natto fermentation.
Article
Chemistry, Multidisciplinary
Fanning Kong, Yilin Mu, Xian Zhang, Qian Lu, Zhizhou Yang, Jinshui Yao, Liyun Zhao
Summary: In this study, a fluorescent probe DPY with active alkynyl groups was designed and prepared for quick, sensitive, and accurate detection of putrescine. DPY showed high selectivity for putrescine and could detect its presence within 30 seconds at room temperature. The probe exhibited good photostability and had a lower detection limit for putrescine compared to the national standard.
Article
Food Science & Technology
Nor Shifa Shuib, Bahruddin Saad
Summary: A new sample pretreatment technique, ISD mu SPE, coupled with high performance liquid chromatography, was used for simultaneous determination of aflatoxins in milk. Online photo-chemical derivatization and optimization of parameters led to high sensitivity and accuracy in the analysis.
Article
Biochemistry & Molecular Biology
Tong Li, Ruiguo Wang, Peilong Wang
Summary: Biogenic amines (BAs) are formed from amino acids and can have various structures. In our study, a method using ultra-performance liquid chromatography-tandem mass spectrometry was developed to rapidly determine 14 BAs in fish samples. The method showed good linearity, low limits of detection, and satisfactory recovery. It was successfully applied to analyze the concentration levels of BAs in different fish.
Article
Food Science & Technology
Zuzana Miskova, Eva Lorencova, Richardos Nikolaos Salek, Tereza Kolackova, Ludmila Travnikova, Anita Rejdlova, Leona Bunkova, Frantisek Bunka
Summary: The aim of this study was to monitor and evaluate the content of eight biogenic amines (BAs) in 232 samples of wines produced in the Central European region (Zone B). PUT, HIS, TYM, and PEA were the most frequently detected BAs in all tested wine samples. The BA content in red wines was generally higher than in rose and white wines, with 9% of the red wine samples having histamine concentrations above the recommended limit of 10 mg/L.