Article
Plant Sciences
Ling Li, Xiaolong Sun, Wencai Yu, Mingchun Gui, Yanfen Qiu, Min Tang, Hai Tian, Guoping Liang
Summary: This study investigates the potential roles of phytohormones in somatic embryogenesis (SE) in rubber plants. Through analyzing the accumulation of phytohormones and gene expression changes in different stages of anther cultures, the study reveals the differential accumulation of phytohormones and their related genes in two different genotypes. The findings suggest the importance of phytohormones in SE in rubber plants.
Article
Agronomy
Waqar Afzal Malik, Imran Mahmood, Abdul Razzaq, Maria Afzal, Ghulam Abbas Shah, Asif Iqbal, Muhammad Zain, Allah Ditta, Saeed Ahmed Asad, Ishfaq Ahmad, Naimatullah Mangi, Wuwei Ye
Summary: The study investigated the tissue culture responses of two wheat genotypes to CuSO4, AgNO3, and their nanoparticles, finding significant differences in embryogenic callus induction and regeneration potential between the genotypes. CuNPs and AgNPs were shown to increase embryogenic callus formation and regeneration efficiency, demonstrating their potential to enhance somatic embryogenesis and regeneration of mature wheat embryos.
GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN
(2021)
Article
Forestry
Abdulrahman A. Alatar, Ahmed A. Qahtan, Eslam M. Abdel-Salam, Mohammad Faisal, Mohamed A. El-Sheikh
Summary: The difficult propagation and extensive grazing exposure of shrub and tree species necessitate finding alternative means of propagation. In vitro micropropagation techniques, such as tissue culture, offer a promising tool for cost-effective and efficient propagation. This study developed a rapid and efficient in vitro multiplication protocol for Maerua crassifolia and demonstrated successful acclimatization and genetic fidelity of the micropropagated plants.
Article
Agronomy
Jinfeng Yang, Yong Soo Kwon, Eun Soo Seong, Myong Jo Kim
Summary: This study found that the air-lift bioreactor had the best proliferation effect on Dendrobium loddigesii protocorm-like bodies (PLBs) compared to other culture methods. The optimal inoculum quantity was determined to be 30 g/vessel, cultured for 28 days in the bioreactor. Analysis of PLB extracts revealed that the ethyl acetate (EtOAc) extract had the highest levels of flavonoids and alkaloids, as well as potent antioxidant activity. Furthermore, the EtOAc extract showed significant antiproliferative effects on human colon carcinoma cells.
Article
Biotechnology & Applied Microbiology
Beiyi Guo, Songjun Zeng, Yuying Yin, Lin Li, Guohua Ma, Kunlin Wu, Lin Fang
Summary: A protocol for inducing PLB development in Paphiopedilum SCBG Huihuang90 was established, revealing significant polarity and cell size gradients within each PLB. The increase in IAA and JA levels, along with decreased GA levels, indicated their correlation with PLB development. Transcriptomic profiles of different PLB developmental stages identified key genes involved in PLB development, with DEGs related to secondary metabolite metabolism and photosynthesis, as well as hormone signaling. This study provides valuable insights into the mechanisms of PLB development in Paphiopedilum.
Article
Biotechnology & Applied Microbiology
Nair S. Yokoya, Wendy A. Stirk, Johannes van Staden
Summary: The use of seaweed extracts as biostimulants is increasing worldwide due to their natural and environmentally friendly properties. This study analyzed the cytokinin-like activity in different species of red seaweeds and identified potential species for use as biostimulants in agriculture.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Izabela Grzegorczyk-Karolak, Katarzyna Hnatuszko-Konka, Marta Krzeminska, Monika A. Olszewska, Aleksandra Owczarek
Summary: The study focuses on exploring the organogenic competence of various growth regulators to develop an efficient protocol for in vitro regeneration of Salvia bulleyana via leaf explants, aiming to maintain high production of active constituents and assess the possibilities of producing therapeutic compounds. Both direct and indirect organogenesis were observed, with the highest regeneration frequency achieved on leaves cultured in specific growth medium. The genetic stability of the obtained organogenic shoot lines was confirmed through ISSR marker-based profiling, indicating potential for stable cultures rich in bioactive metabolites.
Article
Biotechnology & Applied Microbiology
Fangmei Zhou, Weipeng Huang, Wen Cheng, Jingjing Lu, Bingqi Zhu, Jie Yu, Zhishan Ding, Qianxing Jin
Summary: This study optimized the medium for induction and differentiation of PLBs from Pleione bulbocodioides, maintaining the DNA hereditary stability and ultrastructural integrity. The optimal medium formulations were identified for inducing protocorms and PLBs, leading to no DNA polymorphisms during the process. This finding is beneficial for efficient micropropagation of Pleione bulbocodioides.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biotechnology & Applied Microbiology
Yan Liang, Xin Xu, Hailong Shen, Meiling Gao, Yan Zhao, Xue Bai
Summary: The embryogenic potential of Korean pine calli decreases with successive subcultures. Calli with high embryogenic potential exhibit designated embryonic structures and mucilaginous extracellular matrix, while calli with low embryogenic potential show disintegrated embryonic structures and degraded cells. The levels and ratios of endogenous phytohormones also play important roles in maintaining embryogenic potential.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biotechnology & Applied Microbiology
Zhaojian Wang, Weimin Jiang, Yingying Liu, Xiaoxi Meng, Xinglong Su, Mengyang Cao, Liping Wu, Nianjun Yu, Shihai Xing, Daiyin Peng
Summary: Protocorm-like bodies (PLBs) of Dendrobium officinale were obtained through tissue culture. The optimal medium formula for PLB propagation was determined. Stems, PLBs, and leaves of D. officinale had the highest content of different bioactive ingredients. Comparative transcriptome analysis revealed differential gene expression related to biosynthetic pathways of polysaccharides, alkaloids, and flavonoids in D. officinale.
Article
Plant Sciences
Munirah Adibah Kamarul Zaman, Azzreena Mohamad Azzeme, Illy Kamaliah Ramle, Nurfazlinyana Normanshah, Noor Azmi Shaharuddin, Syahida Ahmad, Siti Nor Akmar Abdullah
Summary: The production of alkaloids in Polyalthia bullata was investigated, and methanolic extracts showed the highest alkaloid content in leaf, stem, and root tissues. The callus grown on MS + 30 mu M 2,4-D exhibited the highest alkaloid accumulation after 6 weeks, indicating the potential of this medium for enhancing alkaloid production in P. bullata callus.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2021)
Article
Biotechnology & Applied Microbiology
Diana Isabel Correa, Edgar Pastene-Navarrete, Marcelo Baeza, Luis Bustamante, Julio Alarcon-Enos
Summary: This study identified the alkaloid composition in bulbs of different species of Amaryllidaceae plants and found that certain combinations of plant growth regulators can increase the production of lycorine-type alkaloids. The results suggest that micropropagation is an effective tool for producing these alkaloids.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biology
Marzena Parzymies, Magdalena Pogorzelec, Katarzyna Glebocka, Elwira Sliwinska
Summary: To save the endangered populations of Salix myrtilloides, in vitro propagation was used to obtain genetically identical plants. Shoot pieces were multiplied in a growth media and then planted into soil. Genetic analysis confirmed the effectiveness of tissue culture in the propagation of the endangered species.
Article
Horticulture
K. Grigoriadou, V. Sarropoulou, N. Krigas, E. Maloupa
Summary: This study successfully established a protocol for the vegetative and in vitro propagation of the Balkan sub-endemic Astragalus suberosus subsp. haarbachii, contributing to its successful ex situ conservation and further sustainable exploitation.
EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Frank L. McFarland, Ray Collier, Nathalie Walter, Brian Martinell, Shawn M. Kaeppler, Heidi F. Kaeppler
Summary: The ability of plant somatic cells to dedifferentiate, form somatic embryos and regenerate whole plants in vitro has been harnessed for both clonal propagation and as a key component of plant genetic engineering systems. Embryogenic culture response is significantly limited, however, by plant genotype in most species. We utilized natural variation among maize inbred lines to genetically map somatic embryo generation potential in tissue culture and identify candidate genes underlying totipotency.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Shabnam Safari, Farzad Nazari, Yavar Vafaee, Jaime A. Teixeira da Silva
Summary: The use of rice husk biochar (RHB) can improve soil properties and nutrient uptake, enhancing the growth and drought tolerance of perennial ryegrass.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Letter
Medicine, General & Internal
Jaime A. Teixeira da Silva
IRISH JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Plant Sciences
Danqi Zeng, Can Si, Jaime A. Teixeira da Silva, Guangyi Dai, Juan Duan, Chunmei He
Summary: This study provides a comprehensive characterization of YABBY genes in the valuable Chinese herb Dendrobium officinale, revealing their crucial roles in flower development and the regulatory network they form with WOX proteins.
Article
Environmental Sciences
Jaime A. Teixeira da Silva, Serhii Nazarovets
Summary: This study assessed the frequency of eight nomenclatural forms of MDA between 2001 and 2021 and proposed the need for a standardized nomenclatural form of MDA in order to improve the accuracy and integrity of literature searches.
INTERNATIONAL JOURNAL OF PHYTOREMEDIATION
(2023)
Article
Medical Ethics
Jaime A. Teixeira da Silva, Maryna Nazarovets
Summary: After a paper is published, serious concerns about the study, authors or background processes may arise, leading to the rapid retraction of the paper by editors-in-chief if there is sufficient evidence of ethical offenses or methodological errors. However, there may be a significant time gap between receiving a report and finding a resolution, during which readers need to be alerted to the potential unreliability of the paper. In such cases, editors can issue an editorial expression of concern (EoC) as an alternative document.
ACCOUNTABILITY IN RESEARCH-ETHICS INTEGRITY AND POLICY
(2023)
Review
Virology
Francesco Chirico, Jaime A. Teixeira da Silva
Summary: Open and transparent communication between a government and its citizens, including scientists and healthcare professionals, is crucial for effective health-related policymaking during the COVID-19 vaccination campaign. Sharing open data and adopting an evidence-based approach can help address vaccine hesitancy caused by misinformation and make health strategies more effective.
Letter
Clinical Neurology
Jaime A. Teixeira A. da Silva
NEUROLOGICAL SCIENCES
(2023)
Article
Plant Sciences
Jaime A. Teixeira A. da Silva
Summary: A recent editorial in Plant Cell Reports confirms adherence to ICMJE authorship clauses but highlights the lack of clarity and equality in author contributions. The paper argues that the veracity of authorship claims cannot be verified solely based on eloquent contribution statements. The responsibility for verification lies with editors and publishers to avoid blind trust in academic publishing.
PLANT CELL REPORTS
(2023)
Article
Information Science & Library Science
Jaime A. Teixeira A. da Silva, Panagiotis Tsigaris
Summary: The International Committee of Medical Journal Editors (ICMJE) recommendations are the dominant guidelines for authorship in academic papers. Large language models (LLMs) and AI, such as ChatGPT, challenge the human-based authorship model due to their ability and versatility. Some journals and publishers have prohibited authorship assignment to AI and LLMs, including ChatGPT, as they are not recognized as valid authors.
LEARNED PUBLISHING
(2023)
Article
Humanities, Multidisciplinary
Jaime Teixeira A. da Silva, Yuki Yamada
Summary: ResearchGate (RG) is a popular social media platform for scientists, researchers, or academics (SRAs), which provides the RG Score as a metric to measure an SRA's academic worth, productivity, and interaction. The precise calculation of the RG Score remains undisclosed, making it a proprietary algorithm. RG announced that it will phase out the RG Score in June 2022. This article examines the literature on the RG Score and highlights its potential bias towards certain aspects, such as questions and answers, rather than the published literature of an SRA. The RG Interest Score is also critiqued. An author-based metric that balances important academic factors while disregarding redundant aspects may benefit SRAs. The RG Score should not be used in isolation, manipulated, or used as a basis for financial schemes.
JOURNAL OF SCHOLARLY PUBLISHING
(2023)
Article
Medical Ethics
Jaime A. Teixeira da Silva, Yuki Yamada
Summary: This article proposes a type of DOI-based manuscript called the author expression of concern (AEOC) that allows authors to formally express their concerns about procedural problems in journals. This formalized format, managed by a neutral third-party arbitrator, aims to ensure fairness and accountability by screening the presented evidence and requiring a formal response from criticized parties.
ACCOUNTABILITY IN RESEARCH-ETHICS INTEGRITY AND POLICY
(2023)
Article
Information Science & Library Science
Jaime A. Teixeira da Silva, Maryna Nazarovets
Summary: Web-based references are crucial in academic literature and communication, and it is important to preserve them to maintain knowledge integrity. Reference rot, which refers to the disappearance or dysfunction of web-based resources and references, remains unresolved. In this article, we propose the Internet Archive as a potential solution, while acknowledging its limitations.
LEARNED PUBLISHING
(2023)
Article
Humanities, Multidisciplinary
Jaime A. Teixeira A. da Silva, Chun-Kai (Karl) Huang, Jose Luis Ortega
Summary: Citations in scientific papers are important for verifying information and supporting statements. Open citation databases allow researchers to independently verify the validity and accuracy of citations, and can also be used to evaluate research performance. Open citations have the potential to benefit careers, funding, and detect abusive citation strategies. However, challenges such as funding and data security need to be addressed.
JOURNAL OF SCHOLARLY PUBLISHING
(2023)
Letter
Health Policy & Services
Jaime A. Teixeira da Silva
HEALTH POLICY AND TECHNOLOGY
(2023)
Article
Information Science & Library Science
Maryna Nazarovets, Jaime A. Teixeira da Silva
Summary: This article discusses the importance of using the Internet Archive to archive journal editorial boards. The authors argue that there are three reasons for doing so: journals may lack formal mechanisms to register changes in the editorial constitution; appreciating changes in the editorial board composition holds historical importance, including changes in gender representation; and changes may be made opaquely to hide information linked to the journal's history. By archiving editorial board pages, a better understanding of the journal's quality assurance can be gained from a historical and bibliometric perspective.
PUBLISHING RESEARCH QUARTERLY
(2023)
