Review
Chemistry, Applied
Lin Yuan, Yingbin Su, Hailin Cong, Bing Yu, Youqing Shen
Summary: BODIPY and its derivative multifunctional small molecule probes are widely used in biomedical materials due to their unique molecular structure, high brightness, and effective anti-tumor effects. The derivatives formed by BODIPY and metal or metal ions can regulate their optical properties and solve the autotoxicity problem of metal drugs in tumor treat-ment. The derivatives formed by combining BODIPY with other molecules provide more choices for integrated diagnosis and treatment. This review focuses on the latest research achievements of BODIPY derivatives in imaging and photodynamic therapeutic effects in aqueous solution or cells.
Article
Biochemical Research Methods
Benjamin C. Campbell, Maria G. Paez-Segala, Loren L. Looger, Gregory A. Petsko, Ce Feng Liu
Summary: This study reports a remarkably stable yellow fluorescent protein named hfYFP and its variants, which exhibit outstanding chemical and thermal stability for various challenging applications. By analyzing the crystal structure of hfYFP, highly stable 405nm-excitable green fluorescent proteins were developed. Furthermore, a fluorescence-assisted protein purification strategy was devised, allowing visualization of all steps of denaturing affinity chromatography using ultraviolet or blue light.
Article
Biochemical Research Methods
Isabell Whiteley, Chenchen Song, Glenn A. Howe, Thomas Knopfel, Christopher J. Rowlands
Summary: A targeted imaging system has been developed for recording stationary samples with high spatiotemporal resolutions. It utilizes sequential illumination of regions of interest and records the signal from the whole field of view onto a single photodetector. The system is cost-effective and can be easily implemented on existing microscopes without compromising their functionality. It was successfully used to record individual action potentials from ASAP-3 expressing neurons in an ex vivo mouse brain slice preparation.
BIOMEDICAL OPTICS EXPRESS
(2023)
Review
Pharmacology & Pharmacy
Paul Young
Summary: Advanced therapy medicinal products have extended the treatment paradigm to gene therapy, which modifies genes for therapeutic effect. Gene therapy has strong growth potential, especially using adeno-associated viruses (AAVs) for targeted delivery. However, manufacturing challenges exist in generating high-quality and efficacious viral vectors. Learning from established processes like monoclonal antibody production and embracing innovative approaches are crucial for scalable and cost-effective solutions. Ultimately, the goal is to turn treatment into a cure.
DRUG DISCOVERY TODAY
(2023)
Review
Pharmacology & Pharmacy
Paul Young
Summary: Advanced therapy medicinal products have expanded the treatment options in the pharmaceutical industry by enabling gene therapy for potentially curative approaches, primarily using adeno-associated viruses (AAVs) for targeted delivery. However, manufacturing challenges exist in ensuring high quality and efficacy of AAV-based gene therapy products. Learning from established manufacturing processes like monoclonal antibodies (mAbs) can help develop scalable and cost-effective solutions. Future innovation and collaboration with regulatory agencies are essential for further advancements in gene therapy manufacturing and achieving the ultimate goal of cure.
DRUG DISCOVERY TODAY
(2023)
Article
Engineering, Electrical & Electronic
Christian Dorize, Sterenn Guerrier, Elie Awwad, Haik Mardoyan, Jeremie Renaudier
Summary: In the past decade, coherent technology has been developed for transmission over core optical networks, leveraging fiber capacity through the use of polarization diversity I/Q modulation and advanced signal processing. Recently, Distributed Acoustic Sensing (DAS) over telecom infrastructure has gained interest, and this paper shows that the combination of coherent solutions and digital processing techniques in current transceiver systems can greatly improve DAS technology by enhancing key performance parameters and coexistence with data traffic.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Tian Han, Jinglun Yang, Rong Miao, Ke Liu, Jing Li, Dan Wang, Taihong Liu, Yu Fang
Summary: A nanofilm-based fluorescent sensor is proposed to directly distinguish methanol from other alcohols without the need for pretreatment. This sensor shows excellent performance in efficiently detecting methanol vapor.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Biochemistry & Molecular Biology
Sellamuthu Anbu, Anup Paul, Kalpana Surendranath, Ahmed Sidali, Armando J. L. Pombeiro
Summary: The sensor NPP demonstrates a significant enhancement in fluorescence emission upon binding with Cu2+ and can be used for both fluorescent and colorimetric detection in an aqueous medium. It was also successfully applied as a fluorescent probe for detecting Cu2+ in live cancer cells, showing recovery of emission intensity upon complex formation and reversible response to Cu2+ and EDTA in solution.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Audrey Picard-Lafond, Dominic Lariviere, Denis Boudreau
Summary: Metal-enhanced fluorescence is a powerful tool that improves the brightness and photostability of target-responsive fluorophores, making it useful in the design of sensitive chemical sensors. Expanding the use of metal-enhanced fluorescence to the detection of low-level inorganic pollutants, such as monitoring mercury in the environment, is an important goal. This study demonstrates the potential of metal-enhanced fluorescence for heavy metal detection through fluorescence sensing, by developing a Hg2+-sensitive nanoprobe.
Article
Agriculture, Dairy & Animal Science
Yaroslav Rzhechitskiy, Anton Gurkov, Nadezhda Bolbat, Ekaterina Shchapova, Anna Nazarova, Maxim Timofeyev, Ekaterina Borvinskaya
Summary: This study shows that the adipose fin of salmonid fish is the optimal choice for implanting optical sensors due to its high transparency and ease of implantation. The study also demonstrates the possibility of acquiring sensor signals without fish anesthesia using a simple hand-held device.
Article
Chemistry, Analytical
Robert Nissler, Julia Ackermann, Chen Ma, Sebastian Kruss
Summary: This article discusses the first examples of chirality-pure SWCNT-based fluorescent biosensors and introduces the method of using aqueous two-phase extraction for purification of SWCNTs. These new sensors can detect and image neurotransmitters, reactive oxygen species, lipids, bacterial motives, and plant metabolites in the near-infrared region, with enhanced bioanalytical performance.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Ines Delfino, Nadia Diano, Maria Lepore
Summary: This paper discusses the dangers of phenolic compounds in the environment and the drawbacks of conventional detection methods, as well as the interest in developing new sensing methods for rapid and easy monitoring of phenolic compound concentrations.
Review
Chemistry, Multidisciplinary
Bhargav D. Mansuriya, Zeynep Altintas
Summary: Carbon dots, with unique attributes such as excellent optical properties and biocompatibility, have gained significant attention and interest in the field of nanotechnology and biomedicine. Their abundant functional groups on surfaces make them easily functionalized, exhibiting significant sensing features such as specificity, selectivity, and multiplex detectability.
Article
Chemistry, Analytical
Yoshio Suzuki
Summary: The study developed an improved method for glycome detection using fluorescent magnetic beads modified with lectins, achieving highly sensitive detection of saccharides or glycoproteins through a novel fluorescence emitter and quencher pair. The results demonstrate the accurate and sensitive detection of lectin-glycome interactions without the need for tedious washing processes, providing a rapid and reliable method for profiling diverse glycoconjugates.
Article
Pharmacology & Pharmacy
Silvia Berisa-Prado, Natalia Vazquez, Manuel Chacon, Mairobi Persinal-Medina, Sergio Alonso-Alonso, Begona Baamonde, Jose F. Alfonso, Luis Fernandez-Vega-Cueto, Jesus Merayo-Lloves, Alvaro Meana
Summary: Manufacture of Advanced Therapy Medicinal Products (ATMP) involves following strict guidelines and Good Manufacturing Practice (GMP), as well as access to a cleanroom fulfilling certain environmental conditions. To reduce costs, ATMP production can be carried out in an isolator, meeting safety assurance standards at a more affordable cost. This small-scale ultra-clean working environment that complies with GMP guidelines could be a solution for the high costs associated with conventional cleanroom ATMP production.
Article
Chemistry, Multidisciplinary
Mohamed M. Elsutohy, Veeren M. Chauhan, Robert Markus, Mohammed Aref Kyyaly, Saul J. B. Tendler, Jonathan W. Aylott
Article
Infectious Diseases
Veeren M. Chauhan, David J. Scurr, Thomas Christie, Gary Telford, Jonathan W. Aylott, David I. Pritchard
PLOS NEGLECTED TROPICAL DISEASES
(2017)
Review
Biophysics
Richard P. Harrison, Veeren M. Chauhan
Review
Biotechnology & Applied Microbiology
Shehnaz Ahmed, Veeren M. Chauhan, Amir M. Ghaemmaghami, Jonathan W. Aylott
BIOTECHNOLOGY LETTERS
(2019)
Article
Parasitology
Veeren M. Chauhan, David Pritchard
Article
Polymer Science
Mohammad Al-Natour, Mohamed D. Yousif, Robert Cavanagh, Amjad Abouselo, Edward A. Apebende, Amir M. Ghaemmaghami, Dong-Hyun Kim, Jonathan W. Aylott, Vincenzo Taresco, Veeren M. Chauhan, Cameron Alexander
Review
Chemistry, Multidisciplinary
Veeren M. Chauhan, Hongyu Zhang, Paul A. Dalby, Jonathan W. Aylott
JOURNAL OF CONTROLLED RELEASE
(2020)
Review
Biotechnology & Applied Microbiology
Claudia Martins, Veeren M. Chauhan, Marco Araujo, Amjad Abouselo, Cristina C. Barrias, Jonathan W. Aylott, Bruno Sarmento
Article
Biotechnology & Applied Microbiology
Birte Hollmann, Mark Perkins, Veeren M. Chauhan, Jonathan W. Aylott, Kim R. Hardie
Summary: Understanding the dynamic environmental microniches of biofilms will help us detect, manage, and exploit these communities, although there is still limited knowledge about the existing microniches. The use of ratiometric fluorescent pH-sensitive nanosensors allows real-time observation of physiological pH changes in biofilms, offering a potential application for identifying therapeutic solutions. This technology can be applied to analyze real-time three-dimensional pH variations in different biofilm formers like opportunistic pathogens and oral pathogens, providing insights into improving oral health.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Article
Chemistry, Analytical
Veeren M. Chauhan, Mohamed M. Elsutohy, C. Patrick McClure, William L. Irving, Neil Roddis, Jonathan W. Aylott
Summary: A multimodal biosensor has been developed for detecting enteroviral nucleic acid sequences, with rapid, accurate, and economical features. The sensor can detect conserved enteroviral nucleic acid sequences and has potential applications in clinical disease evaluation and overcoming antimicrobial resistance.
Correction
Polymer Science
Mohamed D. Yousif, Mohammad A. Al-Natour, Robert Cavanagh, Amjad Abouselo, Edward A. Apebende, Amir Ghaemmaghami, Dong-Hyun Kim, Jonathan W. Aylott, Vincenzo Taresco, Veeren M. Chauhan, Cameron Alexander
Article
Chemistry, Multidisciplinary
Claudia Martins, Veeren M. Chauhan, Amjad A. Selo, Mohammad Al-Natour, Jonathan W. Aylott, Bruno Sarmento
REACTION CHEMISTRY & ENGINEERING
(2020)
Article
Chemistry, Multidisciplinary
Richard P. Harrison, Veeren M. Chauhan, David Onion, Jonathan W. Aylott, Virginie Sottile
Article
Chemistry, Multidisciplinary
Mohamed M. Elsutohy, Amjad Selo, Veeren M. Chauhan, Saul J. B. Tendler, Jonathan W. Aylott