Rose petal and P123 dual-templated macro-mesoporous TiO2 for a hydrogen peroxide biosensor

In this work, highly ordered macro-mesoporous TiO2 has been successfully synthesized using fresh rose petals and P123 (EO20PO70EO20) as dual templates through a simple soaking and calcining process. Characterization of the as-prepared TiO2 indicated that the mesoporous structure of the TiO2 was highly ordered, with a pore diameter of approximately 3 nm. After electrodeposition of Pt nanoparticles onto the TiO2 as an electron transfer enhancer and the immobilization of horseradish peroxidase (HRP) onto the TiO2-modified electrode, a biosensor for detecting hydrogen peroxide (H2O2) was realized. This biosensor showed a wide linear detection range from 5 mu M to 8 mM and a low detection limit of 1.65 mu M with good stability and high selectivity, suggesting that the sensor is well-suited for the detection of H2O2. (C) 2017 Elsevier B.V. All rights reserved.