Article
Food Science & Technology
Yi Ma, Liu Cui, Meng Wang, Qiuli Sun, Kaisheng Liu, Jufang Wang
Summary: This study compared the lysis efficiency of different Protein E variants in E. coli, resulting in improved production of high-quality BGs. The use of pLysS plasmid led to nearly 100% lysis efficiency, yielding BGs with higher quality compared to conventional methods. This novel approach was successfully applied to produce high-quality bacterial ghosts for potential biomedical applications.
Article
Construction & Building Technology
Jung Hoon Lee, Hwan Kim, Hyoung-Il Kim, Kook Hyun Yu
Summary: Devices for the filtration and sterilization of indoor spaces have been widely used due to the COVID-19 outbreak. This study aimed to identify important factors for evaluating air purifier efficiency in large chambers. The distribution characteristics and natural reduction of airborne viruses were investigated, and it was found that the airborne-virus removal efficiency should be evaluated after 40 minutes for accuracy. Additionally, the sampling efficiencies of different samplers were compared, with the impactor showing the highest efficiency and lowest standard deviation.
INDOOR AND BUILT ENVIRONMENT
(2023)
Article
Virology
Matthew Brown, Alex Hall, Henriett Zahn, Marcia Eisenberg, Stephen Erickson
Summary: This study evaluated a cocktail of luciferase bacteriophage reporters for functionality in a matrix containing human serum and Staphylococcus aureus. Serum was found to inhibit the signal and decrease sensitivity, and immunoglobulin G was identified as an inhibitory component. Recombinant protein A improved the signal significantly.
Article
Multidisciplinary Sciences
Brandon A. Berryhill, Douglas L. Huseby, Ingrid C. McCall, Diarmaid Hughes, Bruce R. Levin
Summary: The study investigates the potential of the bacteriophage PYOSa for treating Staphylococcus aureus infections, showing promising results in terms of host range and killing rate. However, issues such as failure to clear bacterial populations and the emergence of small-colony variants need to be addressed before clinical application.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Jian Song, Jin Zhao, Xuejun Cai, Shengle Qin, Zexin Chen, Xiaodong Huang, Runfeng Li, Yutao Wang, Xinhua Wang
Summary: Lianhuaqingwen capsule has been proven to have antiviral and antibacterial effects, reducing lung damage and inflammatory mediators like IL-6, IL-1 beta, IL-10, TNF-alpha, IFN-beta, MCP-1, and RANTES. It shows promise in treating influenza secondary bacterial infection.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Virology
Elizabeth T. Ogunbunmi, Aaron P. Roznowski, Bentley A. Fane
Summary: Most icosahedral viruses condense their genomes into volume-constrained capsids, but ssDNA viruses have specific genome biosynthesis and packaging mechanisms. The packaging of ssDNA genomes combines elements from both dsDNA and ssRNA systems, potentially affecting both packaging and postpackaging phenomena.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sha Liu, Karen Hon, George Spyro Bouras, Alkis James Psaltis, Keith Shearwin, Peter-John Wormald, Sarah Vreugde
Summary: The isolated Staphylococcus aureus phages showed significant bactericidal activity against the biofilm form and a broad host range, with low mutation rate, indicating potential for further development as a treatment alternative to antibiotics for Methicillin-Resistant Staphylococcus Aureus (MRSA) infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Jonathan Save, Yok-Ai Que, Jose M. Entenza, Camille Kolenda, Frederic Laurent, Gregory Resch
Summary: This study evaluated the potential of phage therapy for the treatment of Staphylococcus aureus infections and found that combining phages with a low-dose antibiotic could be a promising strategy for the treatment of S. aureus infective endocarditis.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Chemistry, Physical
Baptiste Arbez, Marion Gardette, Christophe Gantzer, Neus Vila, Isabelle Bertrand, Sofiane El-Kirat-Chatel
Summary: This study investigates the adhesion of phage 187 on living Staphylococcus aureus cells using atomic force microscopy-based single-particle force spectroscopy. The addition of N-acetyl-D-glucosamine was found to decrease phage adhesion, suggesting its important role in phage 187 infection. The research also successfully identifies a major receptor required for phage 187 infection of S. aureus.
Article
Plant Sciences
Jin Zhao, Yutao Wang, Xiaodong Huang, Qinhai Ma, Jian Song, Xiao Wu, Hongxia Zhou, Yunceng Weng, Zifeng Yang, Xinhua Wang
Summary: LSW may prevent S. aureus adherence to influenza virus-infected A549 cells by inhibiting CEACAM1 expression. LSW significantly reduces the induction of inflammatory mediators by PR8 infection and suppresses the upregulation of related signaling proteins induced by viruses and bacteria in A549 cells. LSW effectively alleviates lung injury caused by viral and secondary bacterial infection.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Chemistry, Analytical
Xiao-Yan Wang, Jian-Yu Yang, Yi-Ting Wang, Hui-Chao Zhang, Ming-Li Chen, Ting Yang, Jian-Hua Wang
Summary: A surface-enhanced Raman scattering (SERS) nanoprobe based on M13 phages was developed for selective detection and inactivation of Staphylococcus aureus. This method allowed for quantification of S. aureus in aqueous medium and showed promising potential for multifunctional platform for simultaneous detection and inactivation of S. aureus, including bactericidal capabilities.
Article
Virology
Fei Teng, Xiaoyu Xiong, Songsong Zhang, Guiwei Li, Ruichong Wang, Lanlan Zhang, Xiaona Wang, Han Zhou, Jiaxuan Li, Yijing Li, Yanping Jiang, Wen Cui, Lijie Tang, Li Wang, Xinyuan Qiao
Summary: The study aimed to evaluate the effectiveness of phage therapy against drug-resistant mastitis caused by S. aureus. Five S. aureus phages were isolated and shown to inhibit bacterial growth, alleviate inflammatory responses, and reduce inflammatory infiltration in the mammary glands.
Article
Virology
Karin R. H. Biggs, Clayton L. Bailes, LuAnn Scott, Holly A. Wichman, Elissa J. Schwartz
Summary: In microbial communities, viruses compete for host cells to infect and evolve inhibitory mechanisms to suppress their competitors, such as the superinfection exclusion mechanism. The Phi X174 bacteriophage shows a potential superinfection inhibition mechanism called the reduction effect, where a plasmid containing a fragment of the Phi X174 genome confers resistance to secondary infections. The results suggest that viral fitness often predicts the winner, but the reduction effect can also provide an advantage in modulating phage-phage competition and facilitating coexistence.
Article
Virology
Abby M. Korn, Andrew E. Hillhouse, Lichang Sun, Jason J. Gill
Summary: This study introduces three novel S. aureus jumbo phages: MarsHill, Madawaska, and Machias, isolated from swine production environments in the US. These phages belong to a new clade of S. aureus myophages with similar genome size and protein-coding genes, sharing characteristics with known jumbo phages of Bacillus sp. The phages possess genes encoding complete virion and nonvirion RNA polymerases, homing endonucleases, and a retron-like reverse transcriptase, with presumed uracil-substituted DNA hindering DNA sequencing. These phages also have the ability to transduce host plasmids and infect human S. aureus isolates, highlighting their potential impact on genetic diversity and horizontal gene transfer.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Yuwen Lu, Yifei Lu, Baobao Li, Jiazhen Liu, Lixin Wang, Lianyang Zhang, Yang Li, Qiu Zhong
Summary: StAP1 phage is a MRSA phage isolated from a Chinese hospital, exhibiting broad-spectrum characteristics and strong lytic effects on various MRSA strains, indicating its potential application in MRSA infection treatment.
FRONTIERS IN MICROBIOLOGY
(2023)