Article
Gastroenterology & Hepatology
Romina Fiorotto, Valeria Mariotti, Shakila Afroz Taleb, Syeda A. Zehra, Mytien Nguyen, Mariangela Amenduni, Mario Strazzabosco
Summary: In this study, researchers successfully altered the polarity of biliary organoids by culturing them in a matrix, thus creating a new organoid culture model. This model can be used to study bile transport, interactions with pathogens, epithelial permeability, cross talk with liver and immune cell types, and the effect of matrix changes on the biliary epithelium, providing important insights into the pathobiology of cholangiopathies.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Gastroenterology & Hepatology
Romina Fiorotto, Valeria Mariotti, Shakila Afroz Taleb, Syeda A. Zehra, Mytien Nguyen, Mariangela Amenduni, Mario Strazzabosco
Summary: By manipulating the extracellular matrix, researchers have successfully generated three-dimensional biliary organoids with normal characteristics of bile duct cells. This model can be used to investigate bile transport, interactions with pathogenic microorganisms, epithelial permeability, cross talk with other liver and immune cell types, and the effect of matrix changes on the biliary epithelium, providing important insights into the pathobiology of cholangiopathies.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Gastroenterology & Hepatology
Surya P. Amarachintha, Reena Mourya, Hiroaki Ayabe, Li Yang, Zhenhua Luo, Xiaofeng Li, Unmesha Thanekar, Pranavkumar Shivakumar, Jorge A. Bezerra
Summary: The study investigated the mechanisms linked to abnormal cholangiocyte development in biliary atresia by generating biliary organoids from liver biopsies. The organoids showed evidence of halted epithelial development and structural defects in cholangiocyte-like cells from biliary atresia. Treatment with EGF and FGF2 resulted in the restoration of differentiation and functional markers, improved cell-cell junction, and decreased epithelial permeability in biliary atresia organoids.
Article
Engineering, Biomedical
Elsa Mazari-Arrighi, Dmitry Ayollo, Wissam Farhat, Auriane Marret, Emilie Gontran, Pascale Dupuis-Williams, Jerome Larghero, Francois Chatelain, Alexandra Fuchs
Summary: Cholangiocytes, biliary epithelial cells, are able to self-organize into spherical cysts with a central lumen naturally. This study demonstrates a biocompatible stereolithographic approach to encapsulate cholangiocytes in hydrogel structures, guiding them to form branched tubular networks. The resulting structures, resembling those found in the biliary tree, show stable organization and epithelial maturation markers without the need for additional supporting materials.
Article
Biotechnology & Applied Microbiology
Ehab O. A. Hafiz, Beyza Bulutoglu, Soheir S. Mansy, Yibin Chen, Hoda Abu-Taleb, Somia A. M. Soliman, Ali A. F. El-Hindawi, Martin L. Yarmush, Basak E. Uygun
Summary: This study demonstrated the successful formation of transplantable hepatic microtissues with functional bile drainage, achieved through the coculturing of rat primary hepatocytes, cholangiocytes, and stromal cells. The hepatocytes maintained viability and functionality within the spheroids for up to 7 days, showing potential for repopulating liver scaffolds for transplantable liver grafts.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Cell Biology
Laura M. Molina, Junjie Zhu, Qin Li, Tirthadipa Pradhan-Sundd, Yekaterina Krutsenko, Khaled Sayed, Nathaniel Jenkins, Ravi Vats, Bharat Bhushan, Sungjin Ko, Shikai Hu, Minakshi Poddar, Sucha Singh, Junyan Tao, Prithu Sundd, Aatur Singhi, Simon Watkins, Xiaochao Ma, Panayiotis Benos, Andrew Feranchak, George Michalopoulos, Kari Nejak-Bowen, Alan Watson, Aaron Bell, Satdarshan P. Monga
Summary: YAP1 plays a key role in bile duct development, while hepatocytes achieve adaptation through reduced metabolic and synthetic function to survive for 8 months, blocking the formation of the biliary tree.
Review
Cell Biology
Tianhao Zhou, Debjyoti Kundu, Jonathan Robles-Linares, Vik Meadows, Keisaku Sato, Leonardo Baiocchi, Burcin Ekser, Shannon Glaser, Gianfranco Alpini, Heather Francis, Lindsey Kennedy
Summary: This article discusses the impact of cholestatic injury and cholangiocyte activity on the progression of NAFLD, as well as provides detailed information on the influence of cholangiocytes and bile acid signaling on NAFLD.
Article
Cell Biology
Sofia Ferreira-Gonzalez, Tak Yung Man, Hannah Esser, Rhona Aird, Alastair M. Kilpatrick, Daniel Rodrigo-Torres, Nicholas Younger, Lara Campana, Victoria L. Gadd, Benjamin Dwyer, Niya Aleksieva, Luke Boulter, Mark T. Macmillan, Yinmiao Wang, Katie J. Mylonas, David A. Ferenbach, Timothy J. Kendall, Wei-Yu Lu, Juan Carlos Acosta, Dominic Kurian, Stephen O'Neill, Gabriel C. Oniscu, Jesus M. Banales, Paul J. Krimpenfort, Stuart J. Forbes
Summary: This study investigated the effects of organ preservation before liver transplantation on liver regeneration. The researchers found that biliary senescence was induced during organ retrieval and exacerbated during static cold storage, leading to impaired biliary regeneration. By using senolytics and machine-perfusion preservation, the researchers were able to prevent this phenotype and reduce the incidence of biliary injury after transplantation.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Gastroenterology & Hepatology
Hanaa A. El-Araby, Magdy A. Saber, Noha M. Radwan, Doha M. Taie, Nermin M. Adawy, Ahmad M. Sira
Summary: In infants with biliary atresia, bile ductular proliferation and fibrosis show significant temporal changes within a short interval. Fibrosis progression is significantly correlated with temporal changes in bile ductular proliferation, suggesting targeting bile ductular proliferation as an adjuvant medical therapy is recommended.
ANNALS OF HEPATOLOGY
(2021)
Article
Genetics & Heredity
Mei-Rong Bai, Hao-Yue Pei, Ying Zhou, Huan-Lei Song, Wei-Hua Pan, Yi-Ming Gong, Wen-Jie Wu, Wen-Wen Yu, Meng-Meng Cui, Bei-Lin Gu, Xun Chu, Wei Cai
Summary: This study aimed to investigate the association between common variants in the JAG1 gene and the risk of biliary atresia (BA) in the Chinese Han population. The study found significant associations between two single nucleotide polymorphisms (rs6077861 and rs3748478) within the JAG1 gene and BA susceptibility. Knockdown of JAG1 homologs in a zebrafish model led to defective biliary development. These findings suggest that JAG1 may be involved in the etiology of BA.
FRONTIERS IN GENETICS
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Federico Pedersoli, Anja Schroder, Markus Zimmermann, Maximilian Schulze-Hagen, Sebastian Keil, Tom Florian Ulmer, Ulf Peter Neumann, Christiane K. Kuhl, Philipp Bruners, Peter Isfort
Summary: This study compared the success, technical complexity, and complication rates of PTBD in patients with dilated vs. nondilated bile ducts. Results showed a higher success rate in patients with dilated ducts, higher bleeding risk in patients with nondilated ducts, and higher technical complexity in patients with dilated ducts.
EUROPEAN RADIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Deepthi Y. Tulasi, Diego Martinez Castaneda, Kortney Wager, Connor B. Hogan, Karel P. Alcedo, Jesse R. Raab, Adam D. Gracz
Summary: Researchers characterized the expression of a Sox9(EGFP) transgene in the liver and identified different levels of green fluorescent protein (GFP) associated with distinct cell types. They found that Sox9(EGFP) levels provide a readout of Yap activity and delineate the heterogeneity of biliary epithelial cells (BECs), providing a tool for assessing subpopulation-specific cellular functions in the liver.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Gastroenterology & Hepatology
Tianhao Zhou, Vik Meadows, Debjyoti Kundu, Konstantina Kyritsi, Travis Owen, Ludovica Ceci, Guido Carpino, Paolo Onori, Eugenio Gaudio, Nan Wu, Shannon Glaser, Burcin Ekser, Gianfranco Alpini, Lindsey Kennedy, Heather Francis
Summary: Bile ducts, being heterogeneous in structure and function, are damaged in primary sclerosing cholangitis (PSC), leading to various pathological changes. Mast cells (MCs) interact with large cholangiocytes during cholestasis, promoting biliary and liver damage. This finding has important implications for the treatment of PSC.
HEPATOLOGY COMMUNICATIONS
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Bei-Ning Qi, Wen-Juan Lv, Jian-Bo Jian, Xiao-Hong Xin, Xin-Yan Zhao, Yuan-Yuan Zhao, Yu-Qing Zhao, Chun-Hong Hu
Summary: Using PCCT-based 3D virtual histopathology, this study investigated the microvascular alterations in the Glisson system of biliary atresia (BA) patients after Kasai portoenterostomy. The results demonstrate that Kasai portoenterostomy has a significant impact on the microvascular structures in the Glisson system and provide insights into the morphological adaptation mechanisms of microvasculature induced by biliary tract dredging in BA disease.
EUROPEAN RADIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Abigail E. Russi, Pranavkumar Shivakumar, Zhenhua Luo, Jorge A. Bezerra
Summary: This study investigates the mechanisms by which different subpopulations of type 2 innate lymphoid cells (ILC2s) regulate the response of biliary epithelial cells to injury. The results demonstrate that natural ILC2s are positively associated with cholangiocyte abundance and that they promote epithelial proliferation through the IL-13/IL-4Rα/STAT6 pathway. This pathway plays a crucial role in maintaining epithelial homeostasis and promoting repair in experimental biliary atresia.
Review
Biochemistry & Molecular Biology
M. T. Ciubuc-Batcu, N. J. C. Stapelberg, J. P. Headrick, G. M. C. Renshaw
Summary: The nervous system relies on mitochondria, and impaired mitochondrial function is associated with major depressive disorder. Modulating mitochondrial function may be a therapeutic target for treating MDD.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Correction
Biochemistry & Molecular Biology
Saowaluk Saisomboon, Ryusho Kariya, Piyanard Boonnate, Kanlayanee Sawanyawisuth, Ubon Cha'on, Vor Luvira, Yaovalux Chamgramol, Chawalit Pairojkul, Wunchana Seubwai, Atit Silsirivanit, Sopit Wongkham, Seiji Okada, Sarawut Jitrapakdee, Kulthida Vaeteewoottacharn
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Pavan Thapak, Zhe Ying, Victoria Palafox-Sanchez, Guanglin Zhang, Xia Yang, Fernando Gomez-Pinilla
Summary: Traumatic brain injury (TBI) impairs cellular energy demand, compromising neuronal function and plasticity. This study demonstrates that the mitochondrial activator humanin (HN) can counteract the reduction in mitochondrial bioenergetics caused by TBI, restore memory function and synaptic protein levels, and suppress inflammation and astrocyte proliferation. HN plays an integral role in normalizing fundamental aspects of TBI pathology.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
M. Paul Murphy, Valeria A. Buzinova, Carrie E. Johnson
Summary: Progress has been made in the treatment of Alzheimer's disease through the development of anti-A beta therapeutics, which have shown modest efficacy in slowing the progression of the disease. However, the puzzling issue remains as to why completely removing A beta does not fully stop the disease.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Review
Biochemistry & Molecular Biology
Yang Zhang, Mengqiu Hao, Xuyang Yang, Su Zhang, Junhong Han, Ziqiang Wang, Hai-Ning Chen
Summary: Colorectal cancer often requires adjuvant therapies to reduce tumor burden, and the efficacy of these therapies is significantly influenced by reactive oxygen species (ROS). ROS-mediated colorectal cancer adjuvant therapies involve multiple mechanisms, and preliminary clinical trials have shown the potential of ROS-manipulating therapy in enhancing treatment outcomes.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Mengxin Li, Xuanzhong Wang, Xuyang Chen, Jinghui Hong, Ye Du, Dong Song
Summary: Pancreatic adenocarcinoma (PAAD) is a common digestive malignant tumor with limited treatment options. This study demonstrates that TGM2 may serve as a marker for treatment and prognosis in pancreatic cancer patients. Co-treatment of low dose cisplatin (DDP) and the TGM2 inhibitor GK921 effectively inhibits PAAD cell viability and proliferation in vitro and in vivo, by inhibiting epithelial-to-mesenchymal transition (EMT) induced by TGM2 and enhancing cell cycle arrest and apoptosis caused by DDP. These findings suggest that the combination of GK921 and DDP holds promise as a treatment for PAAD patients.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Liaoran Niu, Qi Wang, Fan Feng, Wanli Yang, Zhenyu Xie, Gaozan Zheng, Wei Zhou, Lili Duan, Kunli Du, Yiding Li, Ye Tian, Junfeng Chen, Qibin Xie, Aqiang Fan, Hanjun Dan, Jinqiang Liu, Daiming Fan, Liu Hong, Jian Zhang, Jianyong Zheng
Summary: This review provides a comprehensive summary of the interaction between cancer cells and macrophages in the tumor microenvironment, and discusses the role of small extracellular vesicles (sEVs) in this process. It also explores the various effects of macrophage-secreted sEVs on tumor malignant transformation, and addresses the therapeutic advancements and challenges associated with these vesicles.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Neha Sawant, Sudhir Kshirsagar, P. Hemachandra Reddy, Arubala P. Reddy
Summary: Depression is a common neuropsychiatric comorbidity in Alzheimer's disease (AD) and other Tauopathies. Selective serotonin reuptake inhibitor (SSRI) treatment, such as Citalopram, not only has anti-depressive and anxiolytic effects, but also helps improve neurogenesis, reduce amyloid burden & Tau pathologies, and neuroinflammation in AD. In this study, Citalopram was found to reduce pathologically pTau level, increase synaptic gene expression and cytoskeletal structure, as well as improve cell survival, mitochondrial respiration, and mitochondrial morphology in cells expressing mutant APP and Tau. These findings suggest that Citalopram could be a promising therapeutic drug for treating depression and AD.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Yueqi Chen, Jiulin Tan, Chuan Yang, Zhiguo Ling, Jianzhong Xu, Dong Sun, Fei Luo
Summary: Bone is a self-healing organ that undergoes continuous regeneration through the cooperation of osteoclasts and osteoblasts. This study used ATAC-seq and RNA-Seq techniques to investigate the chromatin accessibility and transcriptomic landscape of osteoblast differentiation and mineralization. The results showed that global chromatin accessibility was extensively improved during osteoblastogenesis. Additionally, several transcription factors including MEF2A, PRRX1, Shox2, and HOXB13 were found to modulate the promoter accessibility of target genes during osteoblast differentiation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Zi-Ran Kang, Shanshan Jiang, Ji-Xuan Han, Yaqi Gao, Yile Xie, Jinxian Chen, Qiang Liu, Jun Yu, Xin Zhao, Jie Hong, Haoyan Chen, Ying-Xuan Chen, Huimin Chen, Jing-Yuan Fang
Summary: The study demonstrates that BCAA metabolism is involved in the development of colorectal cancer (CRC). BCAT2 deficiency promotes CRC progression by inhibiting BCAA metabolism and chronically activating the mTORC1 pathway.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Chao Zheng, Lingling Liu, Caiyun Liu, Fengna Chu, Yue Lang, Shan Liu, Yan Mi, Jie Zhu, Tao Jin
Summary: Inducing tolerogenic dendritic cells (tDCs) with low RelB expression could effectively alleviate symptoms and reduce immune cell infiltration and demyelination in experimental autoimmune encephalomyelitis (EAE) mouse model.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Hang Lam Li, Simei Go, Jung-Chin Chang, Arthur Verhoeven, Ronald Oude Elferink
Summary: This review highlights the distinct characteristics and crucial role of soluble adenylyl cyclase (sAC) in cellular processes, as well as recent significant advancements in the field of sAC research.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
M. Seco-Cervera, D. Ortiz-Masia, D. C. Macias-Ceja, S. Coll, L. Gisbert-Ferrandiz, J. Cosin-Roger, C. Bauset, M. Ortega, B. Heras-Moran, F. Navarro-Vicente, M. Millan, J. V. Esplugues, S. Calatayud, M. D. Barrachina
Summary: The study revealed the presence of resistance to apoptosis in complicated ileal Crohn's disease, with PDGFB inducing an ETS1-mediated resistance to apoptosis associated with an inflammatory and fibrogenic pattern of expression in intestinal fibroblasts. Potential targets against ileal fibrosis include PDGFRB, IL1R1, or MCL1.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Review
Biochemistry & Molecular Biology
Yunmeng Wang, Ping Cheng
Summary: Oncolytic viruses (OVs) are emerging as therapeutically relevant anticancer agents, especially when combined with genetically modified bispecific T cell engagers (BiTEs). This combination strategy can overcome the limitations of BiTEs alone and provide targeted cytotoxicity to solid tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Stephanie Tannous, Hassan Y. Naim
Summary: Congenital sucrase-isomaltase deficiency (CSID) is an autosomal recessive disorder caused by variants in the SI gene. A frameshift mutation called c.273_274delAG (p.Gly92Leufs*8) has been identified in CSID patients in Greenlandic population, which leads to loss of digestive function of SI. Surprisingly, the truncated mutant can still be located on the cell surface and interacts with wild type SI, negatively affecting its enzymatic function. Furthermore, heterozygote carriers of this mutation may also exhibit CSID symptoms.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)