期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1859, 期 2, 页码 110-118出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2017.10.006
关键词
MICROCIN J25; Respiratory chain; Cytochrome; ROS
资金
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [PIP 0779, PIP 06906 CO]
- Agenda Nacional de Promotion Cientffica y Tecnologica (ANPCyT) [PICT 2998]
- Universidad Nacional de Tucuman (UNT) [PIUNT D548/1]
Microcin J25 has two targets in sensitive bacteria, the RNA polymerase, and the respiratory chain through inhibition of cellular respiration. In this work, the effect of microcin J25 in E. coli mutants that lack the terminal oxidases cytochrome bd-I and cytochrome bo(3) was analyzed. The mutant strains lacking cytochrome bo(3) or cytochrome bd-I were less sensitive to the peptide. In membranes obtained from the strain that only expresses cytochrome bd-I a great ROS overproduction was observed in the presence of microcin J25. Nevertheless, the oxygen consumption was less inhibited in this strain, probably because the oxygen is partially reduced to superoxide. There was no overproduction of ROS in membranes isolated from the mutant strain that only express cytochrome bo(3) and the inhibition of the cellular respiration was similar to the wild type. It is concluded that both cytochromes bd-I and bo(3) are affected by the peptide. The results establish for the first time a relationship between the terminal oxygen reductases and the mechanism of action of microcin J25.
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