Article
Cell Biology
Robert C. C. Cail, Cyna R. R. Shirazinejad, David G. G. Drubin
Summary: Cail et al. demonstrate that induced nanoscale membrane curvature can recruit endocytic proteins and produce cargo-laden vesicles without the coat protein clathrin. However, the induced curvature does not bypass AP2 and FCHo1/2 functions. This work suggests that the essential function of clathrin is to physically stabilize the curvature of the evolving vesicle.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Plant Sciences
Deng Chen, Hong Hu, Wenhui He, Shimei Zhang, Mengxi Tang, Shikun Xiang, Caiyun Liu, Xuan Cai, Ahmed Hendy, Muhammad Kamran, Hao Liu, Lu Zheng, Junbing Huang, Xiao-Lin Chen, Junjie Xing
Summary: The PAL1 gene plays a crucial role in endocytosis in the plant-pathogenic fungus Magnaporthe oryzae, regulating appressorium formation and maturation. Through interactions with endocytosis proteins and modulation of cAMP and Pmk1 signaling pathways, Pal1 influences various cellular processes critical for successful infection.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Biophysics
Maria Hoyer, Alvaro H. Crevenna, Jose Rafael Cabral Correia, Andrea G. Quezada, Don C. Lamb
Summary: This study used zero-mode waveguides to directly observe the dynamic nucleation process of actin filaments mediated by gelsolin. The stability of actin dimers determines whether elongation occurs and a conformational change in actin associated with filament formation can prevent elongation.
BIOPHYSICAL JOURNAL
(2022)
Article
Microbiology
T. Essock-Burns, B. D. Bennett, D. Arencibia, S. Moriano-Gutierrez, M. Medeiros, M. J. McFall-Ngai, E. G. Ruby
Summary: This study demonstrates that quorum-sensing regulation by the Vibrio fischeri population induces a tissue phenotype that promotes the retention of this extracellular symbiont within the light organ of its host. Actin polymerization is identified as the primary mechanism underlying constriction, and host responses to the presence of symbionts change as a function of tissue maturation.
Article
Multidisciplinary Sciences
Seth Donoughe, Jordan Hoffmann, Taro Nakamura, Chris H. Rycroft, Cassandra G. Extavour
Summary: The blastoderm formation is a crucial stage of early animal development, and different insects have different cellular behaviors and nuclear movements during this process. A study on the cricket Gryllus bimaculatus reveals that it has a unique solution for blastoderm formation, with nuclear divisions and movements being heterogeneous in space and time and correlated with local nuclear density. A geometric model based on asymmetric pulling forces on nuclei is introduced to explain the patterns of nuclear speeds and orientations. This study sheds light on the cellular and nuclear dynamics during the early stages of insect embryo development.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Javier Lizarrondo, David P. Klebl, Stephan Niebling, Marc Abella, Martin A. Schroer, Haydyn D. T. Mertens, Katharina Veith, Roland Thuenauer, Dmitri Svergun, Michal Skruzny, Frank Sobott, Stephen P. Muench, Maria M. Garcia-Alai
Summary: This study reveals the structure of a 16-mer complex formed by the endocytic coat adaptors Sla2 and Ent1 with the plasma membrane anchor PIP2 in yeast cells. Detailed mutagenesis analysis of the complex interfaces delineates the key interactions for complex formation and its biological relevance in cell growth. The study also shows that the ANTH and ENTH domains have evolved to rapidly assemble in the presence of PIP2, providing insights into clathrin-mediated endocytosis.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Dongmei Liu, Muriel Mari, Xia Li, Fulvio Reggiori, Susan Ferro-Novick, Peter Novick
Summary: This study discovered a process called ER-phagy, which selectively delivers fragments of the endoplasmic reticulum to the lysosome or vacuole in response to starvation or the accumulation of misfolded proteins. The loss of specific genes and proteins blocks the association between the autophagy receptor and the assembly scaffold protein, and a membrane contact site module is also found to be involved in ER-phagy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Steven Z. Chou, Moon Chatterjee, Thomas D. Pollard
Summary: By analyzing the images collected by electron cryo-microscopy, we reconstructed the structure of actin filament branch junctions formed by fission yeast Arp2/3 complex. The formation of this structure requires a conformational change of the Arp2/3 complex and the acquisition of a new buried surface area to maintain the stability of the branch.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Klaas Yperman, Anna C. Papageorgiou, Romain Merceron, Steven De Munck, Yehudi Bloch, Dominique Eeckhout, Qihang Jiang, Pieter Tack, Rosa Grigoryan, Thomas Evangelidis, Jelle Van Leene, Laszlo Vincze, Peter Vandenabeele, Frank Vanhaecke, Martin Potocky, Geert De Jaeger, Savvas N. Savvides, Konstantinos Tripsianes, Roman Pleskot, Daniel Van Damme
Summary: This study elucidated the structural and functional roles of the EH domains of the TPC subunit AtEH1/Pan1 in plant Clathrin-mediated endocytosis (CME). The research integrated high-resolution structural information obtained by X-ray crystallography and NMR spectroscopy with all-atom molecular dynamics simulations to provide insights into the function of both EH domains. The study revealed distinct but complementary roles of the EH domains of AtEH/Pan1 in plant CME and connected the internalization of SCAMP5 to the TPLATE complex.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Emily H. Stoops, Michael A. Ferrin, Danielle M. Jorgens, David G. Drubin
Summary: This research demonstrates the sequence recruitment of core endocytic proteins and regulatory proteins, as well as the assembly of actin networks in live cells. The study shows that supported lipid bilayers coated with purified yeast WASP recruit downstream endocytic proteins and assemble actin networks. The actin-driven vesicle generation could be a precursor for various vesicle forming processes in different cellular environments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Amr Abouelezz, Leonardo Almeida-Souza
Summary: In this review, the latest research progress on the endocytic cytoskeleton is summarized, and the physiological significance and unresolved questions of the endocytic cytoskeleton are discussed.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Maria P. Kotini, Miesje M. van der Stoel, Jianmin Yin, Mitchell K. Han, Bettina Kirchmaier, Johan de Rooij, Markus Affolter, Stephan Huveneers, Heinz-Georg Belting
Summary: Blood vessel morphogenesis is driven by coordinated endothelial cell behaviors. Active remodeling of cell-cell junctions promotes cellular plasticity while preserving vascular integrity. Lumen expansion in angiogenic sprouts is accompanied by the formation of transient finger-shaped junctions. Blood pressure positively regulates the formation of junctional fingers, while flow inhibition prevents their formation. Mechanotransduction protein vinculin is recruited to junctional fingers, and its genetic deletion prevents finger formation. Endothelial cells employ force-dependent junctional remodeling to counteract external forces and maintain vascular integrity during sprouting angiogenesis.
Article
Cell Biology
Ross T. A. Pedersen, Aaron Snoberger, Serapion Pyrpassopoulos, Daniel Safer, David G. Drubin, E. Michael Ostap
Summary: The study demonstrates that the yeast myosin-1 Myo5 is more likely to generate power than to serve as a force-sensitive anchor in cells. This finding has implications for Myo5's role in clathrin-mediated endocytosis.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Cell Biology
Joshua Okletey, Dimitrios Angelis, Tia M. Jones, Cristina Montagna, Elias T. Spiliotis
Summary: This study identifies SEPT9_i1 as a component of breast cancer invadopodia, which play a role in the interaction between the nuclear envelope and the extracellular matrix (ECM) degrading structures. Depletion of SEPT9_i1 reduces invadopodium formation and leads to deformed nuclei. The plasticity of the nucleus is believed to be associated with cancer cell invasion.
Article
Multidisciplinary Sciences
Nikolas Hundt, Daniel Cole, Max F. Hantke, Jack J. Miller, Weston B. Struwe, Philipp Kukura
Summary: This study uses mass photometry to investigate the polymerization process of actin and finds that the traditional nucleation-based models cannot explain the observed distribution of actin oligomers. Instead, the key step of filament formation is a slow transition between different states of an actin filament mediated by cation exchange or ATP hydrolysis. These findings have important implications for understanding the mechanism of actin nucleation and studying protein assembly at the molecular level.
Article
Geriatrics & Gerontology
Hrvoje Augustin, Kieran McGourty, Marcus J. Allen, Jennifer Adcott, Chi Tung Wong, Emmanuel Boucrot, Linda Partridge
NEUROBIOLOGY OF AGING
(2018)
Review
Cell Biology
Antonio P. A. Ferreira, Emmanuel Boucrot
TRENDS IN CELL BIOLOGY
(2018)
Article
Microbiology
Charlotte Ford, Andrea Nans, Emmanuel Boucrot, Richard D. Hayward
Article
Cell Biology
Laura Chan Wah Hak, Shaheen Khan, Ilaria Di Meglio, Ah-Lai Law, Safa Lucken-Ardjomande Hasler, Leonor M. Quintaneiro, Antonio P. A. Ferreira, Matthias Krause, Harvey T. McMahon, Emmanuel Boucrot
NATURE CELL BIOLOGY
(2018)
Review
Cell Biology
Claudia Hinze, Emmanuel Boucrot
JOURNAL OF CELL SCIENCE
(2018)
Review
Biochemistry & Molecular Biology
Alessandra Casamento, Emmanuel Boucrot
BIOCHEMICAL JOURNAL
(2020)
Article
Immunology
Daniel Janman, Claudia Hinze, Alan Kennedy, Neil Halliday, Erin Waters, Cayman Williams, Behzad Rowshanravan, Tie Zheng Hou, Shane Minogue, Omar S. Qureshi, David M. Sansom
Summary: CTLA-4 is distributed across different compartments marked by Rab GTPases, and manipulation of these GTPases affects its expression and trafficking. LRBA deficiency leads to ineffective recycling of CTLA-4 and degradation.
Article
Immunology
Alan Kennedy, Erin Waters, Behzad Rowshanravan, Claudia Hinze, Cayman Williams, Daniel Janman, Thomas A. Fox, Claire Booth, Anne M. Pesenacker, Neil Halliday, Blagoje Soskic, Satdip Kaur, Omar S. Qureshi, Emma C. Morris, Shinji Ikemizu, Christopher Paluch, Jiandong Huo, Simon J. Davis, Emmanuel Boucrot, Lucy S. K. Walker, David M. Sansom
Summary: CD28 and CTLA-4 play important roles in regulating T cell immunity. The study found that CTLA-4 captures ligands through transendocytosis, and CD80 leads to ubiquitination and degradation of CTLA-4, while CD86 allows CTLA-4 to detach and recycle back to the cell surface. Clinically relevant mutations disrupt CD86 transendocytosis and are associated with autoimmune diseases.
Article
Cell Biology
Thomas Andrew Fox, Benjamin Christopher Houghton, Lina Petersone, Erin Waters, Natalie Mona Edner, Alex McKenna, Olivier Preham, Claudia Hinze, Cayman Williams, Adriana Silva de Albuquerque, Alan Kennedy, Anne Maria Pesenacker, Pietro Genovese, Lucy Sarah Kate Walker, Siobhan Oisin Burns, David Michael Sansom, Claire Booth, Emma Catherine Morris
Summary: A gene editing strategy was designed to insert the CTLA-4 cDNA into primary human T cells, restoring CTLA-4 expression and demonstrating the feasibility of using T cell gene therapy for CTLA-4 insufficiency.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Alan Kennedy, Maximillian A. Robinson, Claudia Hinze, Erin Waters, Cayman Williams, Neil Halliday, Simon Dovedi, David M. Sansom
Summary: CTLA-4 and PD-1 are immune checkpoint receptors targeted in cancer treatment. A recent discovery shows that the interaction between their respective ligands, CD80 and PD-L1, can block PD-L1/PD-1 binding and inhibit PD-L1 functions. The internalization of CD80 results in the recovery of PD-L1 availability, while CD80/PD-L1 complexes remain on the plasma membrane. CTLA-4 modulates PD-L1:PD-1 interactions by controlling CD80.
Article
Immunology
Lina Petersone, Chun Jing Wang, Natalie M. Edner, Astrid Fabri, Spyridoula-Angeliki Nikou, Claudia Hinze, Ellen M. Ross, Elisavet Ntavli, Yassin Elfaki, Frank Heuts, Vitalijs Ovcinnikovs, Andrea Rueda Gonzalez, Luke P. Houghton, Hannah M. Li, Yang Zhang, Kai-Michael Toellner, Lucy S. K. Walker
Summary: Petersone et al. investigate the regulation of germinal centers (GC) by IL-21 and uncover important roles of IL-21 in promoting the selection of light zone GC B cells and controlling the proliferation of dark zone GC B cells through upregulation of cyclin D3. The study shows that IL-21 shapes the size and polarization of autoimmune-induced and immunization-induced GC. IL-21 receptor deficiency leads to smaller and skewed GCs towards a light zone GC B cell phenotype, and IL-21 is crucial for the selection of light zone GC B cells for entry into the dark zone. The study also reveals the link between IL-21 signaling and GC dark zone formation through regulation of Foxo1.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Review
Cell Biology
Shigeki Watanabe, Emmanuel Boucrot
CURRENT OPINION IN CELL BIOLOGY
(2017)