期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 495, 期 3, 页码 2350-2355出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2017.12.114
关键词
Lung cancer; IncRNA-PVT1-5; miR-126; SLC7A5; Competing endogenous RNA
资金
- National Natural Science Foundation of China [81460435, 81372360]
- Key Project of the Yunnan Applied Basic Research Plan [2014FA022]
- Science Research Foundation of Yunnan Province [2017FB138]
- Science Research Foundation of the Yunnan Province Education Department [2016ZZX014]
Dysregulated long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play key roles in the development of human cancers. The IncRNA plasmacytoma variant translocation 1 (PVT1) is reported to be an oncogene in a variety of cancers. However, the roles of PVT1-5 and its related miRNAs in lung cancer are poorly understood. In this study, we found that PVT1-5 expression was significantly increased in lung cancer tissues and cell lines. By using biotin-labeled IncRNA-PVT1-5 probe for miRNA in vivo precipitation (miRIP) in lung cancer cells and dual-luciferase reporterassays, we identified that miR-126 was associated with IncRNA-PVT1-5. Furthermore, knockdown of IncRNA-PVT1-5 in cells could down regulate the expression of SLC7A5, the target of oncogenic miR-126, resulting in the cell proliferation. Conversely, inhibiting the expression of miR-126 markedly increased the expression of SLC7A5 and alleviated cell proliferation inhibition. Thus, our results indicated that IncRNA-PVT1-5 may function as a competing endogenous RNA (ceRNA) for miR-126 to promote cell proliferation by regulating the miR126/SLC7A5 pathway, suggesting that IncRNA-PVT1-5 plays a crucial role in lung cancer progression and IncRNA-PVT1-5/miR-126/SLC7A5 regulatory network may shed light on tumorigenesis in lung cancer. (C) 2017 Published by Elsevier Inc.
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