期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 503, 期 4, 页码 2376-2379出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2018.06.164
关键词
Rice; Plastid transformation; Green fluorescent protein; Homoplastomic selection
资金
- National Natural Science Foundation of China [31571754, 31501235]
- Fund for Distinguished Young Scholars from Department of Jilin Province Science and Technology [20170520075JH]
- Agricultural Science and Technology Innovation Project of Jilin Province [CXGC2018ZY029]
- Talent Fund from Department of Human Resources and Social Security of Jilin Province [C72330002]
Rice is one of the most important cereal crops and its biotechnology has been pursued to meet the food demand of ever-growing global population. Rice plastid transformation has been a great challenge to achieve homoplastomic plants due to its low efficiency of regeneration. In this experiment, Japonica rice line 19 was chosen to be the receptor for plastid transformation. A vector harboring smGFP gene was constructed and transferred into rice plastid genome by bombardment. The resistant callus was obtained after long-lasting multiple selections and proved to be in homoplastomic status by molecular testing. The plantlet was regenerated from homoplastomic callus and grown to seeding stage. This is the first case so far to achieve the homoplastomic rice and will be helpful to transform plastid genome of monocotyledonous crops with recalcitrant nature. (C) 2018 Elsevier Inc. All rights reserved.
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