Article
Medicine, Research & Experimental
Zili He, Jiqing Du, Yu Zhang, Yitie Xu, Qian Huang, Qingwei Zhou, Min Wu, Yao Li, Xie Zhang, Hongyu Zhang, Yuepiao Cai, Keyong Ye, Xiangyang Wang, Yingze Zhang, Qi Han, Jian Xiao
Summary: Increasing evidence suggests that defects in autophagy and autophagy-lysosomal pathway (ALP) may contribute to endothelial barrier disruption following acute traumatic spinal cord injury (SCI). This study investigates whether Kruppel-like factor 2 (KLF2) coordinates ALP in endothelial cells in SCI. The results demonstrate that KLF2 is a key contributor to SCI-mediated ALP dysfunction and blood-spinal cord barrier (BSCB) disruption, and could be a promising pharmacological target for SCI management and treatment.
Article
Biochemistry & Molecular Biology
Tao Jiang, Tao Qin, Peng Gao, Zhiwen Tao, Xiaowei Wang, Mengyuan Wu, Jun Gu, Bo Chu, Ziyang Zheng, Jiang Yi, Tao Xu, Yifan Huang, Hao Liu, Shujie Zhao, Yongxin Ren, Jian Chen, Guoyong Yin
Summary: The expression of SIRT1 in spinal cord endothelial cells is decreased after spinal cord injury (SCI). SIRT1 has the ability to reduce endothelial reactive oxygen species production and protect endothelial barrier function, indicating its potential as a therapeutic target for promoting functional recovery against blood-spinal cord barrier (BSCB) disruption following SCI.
Article
Medicine, Research & Experimental
Bo Xu, Jiaqi Fang, Jianguang Wang, Xuehan Jin, Shengfu Liu, Kaihang Song, Ping Wang, Junjian Liu, Shuhao Liu
Summary: This study investigated the protective effect of edaravone on blood spinal cord barrier (BSCB) disruption after spinal cord injury (SCI). The results showed that edaravone treatment promoted functional recovery, improved vascular damage, and up-regulated BSCB-associated proteins in rats with SCI. In vitro experiments also demonstrated that edaravone improved cell viability, restored intercellular junctions, and promoted angiogenic activities. The underlying mechanism may involve the improvement of autophagy and the phosphorylation of RIP1/RIP3/MLKL. Overall, edaravone can be a potential treatment option for SCI.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Zhou Feng, Lingxia Min, Liang Liang, Beike Chen, Hui Chen, Yi Zhou, Weiwei Deng, Hongliang Liu, Jingming Hou
Summary: It has been discovered that infiltrated neutrophils in spinal cord injury produce NETs, promoting neuroinflammation and blood-spinal cord barrier disruption, exacerbating SCI. Inhibition or disruption of NETs formation can alleviate damage and promote functional recovery after SCI.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Li Deng, Jun Qiao Lv, Lin Sun
Summary: This study systematically analyzed the experimental treatments and their mechanisms for reducing blood spinal cord barrier (BSCB) injury in the early stage of traumatic spinal cord injury (t-SCI). BMSC-Exos, which inhibit matrix metalloproteinases (MMPs) expression, are currently the most effective therapeutic modality for alleviating BSCB damage. In addition, the regulation of MMPs, the Akt pathway, and the ER stress pathway play important roles in alleviating BSCB injury.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Guang Kong, Wu Xiong, Cong Li, Chenyu Xiao, Siming Wang, Wenbo Li, Xiangjun Chen, Juan Wang, Sheng Chen, Yongjie Zhang, Jun Gu, Jin Fan, Zhengshuai Jin
Summary: This study explores the influence of Treg cells on the recovery of BSCB after spinal cord injury (SCI) and its underlying mechanism. The findings suggest that Treg cell-derived exosomes can affect BSCB integrity and motor function by regulating the expression of IRAK1. This provides novel insights for functional repair and inflammation limitation after SCI.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Immunology
Rubing Zhou, Junzhao Li, Zhengyang Chen, Ruideng Wang, Yin Shen, Rong Zhang, Fang Zhou, Yong Zhang
Summary: Blood-spinal cord barrier (BSCB) disruption is a crucial event in spinal cord injury (SCI), allowing harmful substances to enter neural tissue and exacerbate secondary damage. The mechanisms of BSCB disruption in the acute phase of SCI remain unclear, leading to a lack of effective clinical treatment strategies.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Engineering, Biomedical
Xin Wang, Baokun Zhang, Zhiheng Chen, Qiang Shi, Erzhu Yang, Jianguang Xu, Xiaofeng Lian
Summary: Following spinal cord injury, the disruption of the blood-spinal cord barrier (BSCB) and hemorrhage lead to progressive secondary injuries. Bazedoxifene, a third-generation estrogen receptor modulator, has been found to inhibit inflammation and blood-brain barrier disruption caused by traumatic brain injury. In this study, a new biomaterial carrier was developed to deliver Bazedoxifene to the site of spinal cord injury and effectively attenuate BSCB disruption through the NF-KB pathway, providing potential therapeutic benefits for spinal cord repair.
ACTA BIOMATERIALIA
(2023)
Review
Biology
Shuo Li, Hoai Thi Phuong Dinh, Yukihiro Matsuyama, Kohji Sato, Satoru Yamagishi
Summary: Traumatic spinal cord injury (SCI) causes physical impairment and psychological devastation through complex pathological processes. The two phases of SCI include primary mechanical damage and secondary injury, which lead to vascular disruption, inflammation, cell death, and scar formation. Secretory factors, such as neurotrophic factors and growth factors, play important roles in nerve regeneration, while the blood-spinal cord barrier (BSCB) is damaged, allowing immune cell infiltration and promoting angiogenesis. This review discusses the therapeutic application and outcomes of these secretory factors in SCI.
Article
Neurosciences
Zhenxin Hu, Tingting Wu, Ziheng Zhou, Yu Zhang, Qiyue Chen, Hanbing Yao, Mengchu Ji, Ge Shen, Chenling Dong, Chengge Shi, Zhixian Huang, Nizhou Jiang, Nan Han, Xiliang Tian
Summary: This study aimed to investigate the effect of asiaticoside on pericytes and the blood-spinal cord barrier (BSCB). The results showed that asiaticoside could inhibit BSCB disruption and enhance functional recovery after spinal cord injury (SCI).
MOLECULAR NEUROBIOLOGY
(2023)
Review
Endocrinology & Metabolism
Rubing Zhou, Junzhao Li, Ruideng Wang, Zhengyang Chen, Fang Zhou
Summary: The neurovascular unit (NVU) represents the close relationship between neurons and blood vessels. However, our understanding of NVU in the spinal cord is limited, and mainly based on knowledge from the brain. In this review, we summarize the current knowledge of NVU and discuss potential approaches to study NVU in the spinal cord. Several unique features maintain the regulated microenvironment in the NVU. Autoregulation and neurovascular coupling ensure proper blood flow based on metabolic demand and neural activation. The blood-central nervous system barrier separates circulating blood from the neural tissue and allows selective exchange of substances. We also discuss the implications of NVU dysfunction in spinal cord injury. This review aims to enhance our understanding of NVU in the spinal cord and provide insights into spinal cord injury.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2023)
Review
Biochemistry & Molecular Biology
Giovanni Schepici, Serena Silvestro, Emanuela Mazzon
Summary: Spinal cord injury (SCI) is a devastating condition that causes permanent motor and sensory deficits. Researchers have been studying potential therapeutic strategies to mitigate the neuro-inflammatory response following injury. Recent studies have shown that exosomes-derived MSCs (MSC-EXOs) have promising effects as an innovative therapy for SCI patients.
Article
Immunology
Chenxi Zhao, Tiangang Zhou, Xiaoqing Zhao, Yilin Pang, Wenxiang Li, Baoyou Fan, Ming Li, Xinjie Liu, Lei Ma, Jiawei Zhang, Chao Sun, Wenyuan Shen, Xiaohong Kong, Xue Yao, Shiqing Feng
Summary: This study provides preclinical information on the administration time window of 2-12 h post-injury for nafamostat in contusive SCI. It reveals that nafamostat functions by inhibiting thrombin-mediated BSCB breakdown and subsequent infiltration of peripheral immune cells.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Immunology
Yong Xie, Zixiang Luo, Wei Peng, Yudong Liu, Feifei Yuan, Jiaqi Xu, Yi Sun, Hongbin Lu, Tianding Wu, Liyuan Jiang, Jianzhong Hu
Summary: This study found that conditional UTX knockout can reduce the permeability of the blood-spinal cord barrier (BSCB) and improve inflammatory response and neurological recovery after spinal cord injury (SCI). Additionally, UTX knockout down-regulates the expression of myosin light chain kinase (MLCK), inhibiting the MLCK/p-MLC pathway and decreasing BSCB permeability.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Clinical Neurology
Rubing Zhou, Junzhao Li, Ruideng Wang, Zhengyang Chen, Fang Zhou
Summary: This study found that moderate systemic therapeutic hypothermia for 4 hours does not have a protective effect on the disrupted blood-spinal cord barrier in early spinal cord injury.
FRONTIERS IN NEUROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)