期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 495, 期 3, 页码 2209-2213出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2017.12.095
关键词
Laccase; Polymerized polyphenol; Macrophage; Racl; Agaricus brasiliensis
资金
- MEXT-Supported Program for the Strategic Research Foundation at Private Universities [51512002]
Various physiologically active effects of polymerized polyphenols have been reported. In this study, we synthesized a polymerized polyphenol (mL2a-pCA) by polymerizing caffeic acid using mutant Agaricus brasiliensis laccase and analyzed its physiological activity and mechanism of action. We found that mL2a-pCA induced morphological changes and the production of cytokines and chemokines in C3H/HeN mouse-derived resident peritoneal macrophages in vitro. The mechanisms of action of polymerized polyphenols on in vitro mouse resident peritoneal cells have not been characterized in detail previously. Herein, we report that the mL2a-pCA-induced production of interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in C3H/HeN mouse-derived resident peritoneal cells was inhibited by treatment with the Racl inhibitor NSC23766 trihydrochloride. In addition, we found that mL2a-pCA activated the phosphorylation Racl. Taken together, the results show that mL2a-pCA induced macrophage activation via Racl phosphorylation-dependent pathways. (C) 2017 Elsevier Inc. All rights reserved.
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