期刊
FEBS LETTERS
卷 589, 期 16, 页码 2058-2065出版社
WILEY
DOI: 10.1016/j.febslet.2015.06.023
关键词
Polyamine; alpha-Difluoromethylornithine (DFMO); Osteogenic differentiation; Small interfering RNA (siRNA); Mesenchymal stem cell
资金
- Ministry of Science and Technology, Taiwan [NSC101-2314-B-309-001-MY3]
- Academic Research Funds of Chang Jung Christian University, Tainan, Taiwan
- Taichung District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, Taichung, Taiwan
Omithine decarboxylase (ODC) is the rate-limiting enzyme for polyamine biosynthesis. Suppression of ODC by its irreversible inhibitor, alpha-difluoromethylornithine (DEMO), or by RNA interference through siRNA, enhanced osteogenic gene expression and alkaline phosphatase activity, and accelerated matrix mineralization of human bone marrow-derived mesenchymal stem cells (hBMSCs). Besides, adipogenic gene expression and lipid accumulation was attenuated, indicating that the enhanced osteogenesis was accompanied by down-regulation of adipogenesis when ODC was suppressed. A decrease in the intracellular polyamine content of hBMSCs during osteogenic induction was observed, suggesting that the level of endogenous polyamines is regulated during differentiation of hBMSCs. This study elucidates the role of polyamine metabolism in the lineage commitment of stem cells and provides a potential new indication for DFMO as bone-stimulating drug. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据