Article
Immunology
Mengyun Wu, Xiu Gao, Yuxu Tang, Wenyan Wu, Ji Zhou, Yu Shao, Chuangli Hao, Yi Yang, Jinping Zhang
Summary: Cbl-b inhibits T cell activation by regulating the expression of miR-99a and miR-125b and their function on the PI3K/AKT pathway. Cbl-b can bind and ubiquitinate SHP-2, which inhibits the activation of SHP-2 deficient T cells. HOXA10, dephosphorylated by SHP-2, regulates the expression of miR-99a/miR-125b and enters the nucleus in Cbl-b deficient T cells stimulated by anti-CD3 antibody alone. This study provides a new explanation for the regulation of T cell activation and potential new ideas for autoimmune diseases and tumor immunotherapies.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xin Yang, Zhenhui Li, Zhijun Wang, Jiaao Yu, Manting Ma, Qinghua Nie
Summary: In this study, the researchers found differential expression of miR-27b-3p and Cbl-b genes in an induced atrophy model. They confirmed that miR-27b-3p can regulate the expression of Cbl-b gene. Cbl-b gene plays an important role in muscle atrophy, as its overexpression can upregulate the expression of marker genes related to muscle atrophy, while silencing Cbl-b expression can alleviate muscle atrophy.
Article
Biotechnology & Applied Microbiology
Hao Jia, Wei Sun, Xiangbo Li, Wenhao Xu
Summary: MEL has been found to effectively inhibit the growth of thyroid cancer and suppress tumor cell proliferation while promoting apoptosis. MEL also regulates the expression of certain genes and proteins to further promote tumor cell apoptosis.
Article
Cell Biology
Jianbing Hou, Minghao Xu, Hongyu Gu, Dakun Pei, Yudong Liu, Pan Huang, Hongbo Chang, Hongjuan Cui
Summary: ZC3H15, an overexpressed protein in GBM, promotes the proliferation, migration, invasion, and tumorigenesis of GBM cells by activating the EGFR signaling pathway. High expression of ZC3H15 is associated with poor patient survival.
CELL DEATH & DISEASE
(2022)
Article
Veterinary Sciences
Florentina Daraban Bocaneti, Gennaro Altamura, Annunziata Corteggio, Oana Irina Tanase, Mihaela Anca Dascalu, Sorin Aurelian Pasca, Ozana Hritcu, Mihai Mares, Giuseppe Borzacchiello
Summary: This study conducted immunohistochemical and biochemical analysis on MMPs and TIMPs in BPV-2 positive bovine cutaneous fibropapillomas, revealing their significant role in tumor development and their unbalanced expression in tumor samples.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Valentin Dichev, Nikolay Mehterov, Maria Kazakova, Rositsa Karalilova, Anastas Batalov, Victoria Sarafian
Summary: This study aimed to investigate a potential regulatory axis involved in the control of CHI3L1 in systemic sclerosis (SSc). The results showed that the expression of miR-30e-5p and miR-30a-5p was significantly downregulated in SSc patients, while the expression of MALAT1 and NEAT1 was upregulated in white blood cells. The findings suggest that MALAT1 and NEAT1 may act as decoys for miR-30e-5p and miR-30a-5p, leading to increased serum levels of CHI3L1 in SSc patients.
Article
Multidisciplinary Sciences
Yiqing Guo, Hua Zheng, Jie Yin, Huaming Wang
Summary: Recent evidence shows that circ-ITCH exerts an inhibitory effect on the progression of papillary thyroid cancer by affecting the circ-ITCH/miR-22-3p/CBL axis. The A allele in rs4911154 is associated with shorter doubling time of thyroid nodules and decreased CBL expression, contributing to increased malignancy.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Caimei Wang, Yiyuan Wang, Congrong Liu, Xiaoyu Meng, Zhongxia Hang
Summary: This study reveals the important role of KNTC1 in the development of cervical cancer and identifies the mechanism by which KNTC1 promotes cervical cancer through MMP9 and MMP2-mediated reduction in tumor cell invasion and migration abilities.
Article
Environmental Sciences
Bharath Kumar Velmurugan, Jen-Tsun Lin, B. Mahalakshmi, Chia-Chieh Lin, Yi-Ching Chuang, Yu-Sheng Lo, Hsin-Yu Ho, Ming-Ju Hsieh, Mu-Kuan Chen
Summary: This study demonstrates that dehydrocrenatidine treatment significantly reduces the motility, migration, and invasion of head and neck cancer cells, decreases MMP-2 expression, and inhibits cell migration by reducing the phosphorylation of ERK1/2 and JNK1/2. Combined treatment with inhibitors enhances the anti-metastatic effects of dehydrocrenatidine.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ah-Young Kwon, Ju-Yeon Jeong, Hyun Park, Sohyun Hwang, Gwangil Kim, Haeyoun Kang, Jin-Hyung Heo, Hye Jin Lee, Tae-Heon Kim, Hee Jung An
Summary: Alterations in miRNA expression are associated with cervical SqCC and metastasis, and miR-30e-5p may serve as an independent poor prognostic factor for survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Qianqian Geng, Huijie Zhao, Zhiqing Zhou, Xiuyuan Chen, Honglin Zhao, Nanzheng Chen, Yu Li, Chunfang Guo, Aimin Yang, David G. Beer, Guoan Chen
Summary: In this study, it was found that circSMARCA5 expression was decreased in lung adenocarcinoma tissues, and silencing of circSMARCA5 inhibited cell proliferation, colony formation, migration, and invasion in lung adenocarcinoma cells. MiR-17-3p was identified to down-regulate EGFR expression by binding directly to EGFR mRNA. These findings suggest that circSMARCA5 may serve as an oncogene by targeting the miR-17-3p-EGFR axis in lung adenocarcinoma.
Article
Environmental Sciences
Kuang-Yu Chou, Po-Chun Chen, An-Chen Chang, Te-Fu Tsai, Hung-En Chen, Chao-Yen Ho, Thomas I-Sheng Hwang
Summary: The study demonstrated that chloroquine and hydroxychloroquine inhibit the migration and invasion of bladder cancer cells by modulating the expression of matrix metalloproteinase-2, and this effect is based on autophagy inhibition.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ombline Conrad, Mickael Burgy, Sophie Foppolo, Aude Jehl, Alicia Thiery, Sebastien Guihard, Romain Vauchelles, Alain C. C. Jung, Jana Mourtada, Christine Macabre, Sonia Ledrappier, Marie-Pierre Chenard, Mihaela-Alina Onea, Aurelien Danic, Thomas Dourlhes, Claire Thibault, Philippe Schultz, Monique Dontenwill, Sophie Martin
Summary: The expression levels of the 3p passenger strands of miR-30a and miR-30e are correlated with the behavior and prognosis of head and neck squamous cell carcinoma. Low expression of miR-30a and miR-30e is associated with adverse prognosis and can potentially be used as biomarkers for diagnosis and treatment of head and neck squamous cell carcinoma. Additionally, miR-30a and miR-30e expression can affect tumor cell behavior and the tumor microenvironment, potentially improving treatment outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Huaying Xie, Tingting Yan, Xinxin Lu, Yueyao Du, Shuguang Xu, Yu Kong, Liangjie Yu, Jian Sun, Liheng Zhou, Jun Ma
Summary: In this study, GLDC was found to be up-regulated in TNBC tissues and its higher expression was correlated with worse prognosis. GLDC overexpression facilitated TNBC cell proliferation, while knockdown of GLDC had the opposite effects. Additionally, miR-30e was identified as a functional upstream regulator of GLDC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu-Hsiang Chang, Yuan-Li Huang, Hsiao-Chi Tsai, An-Chen Chang, Chih-Yuan Ko, Yi-Chin Fong, Chih-Hsin Tang
Summary: This study aims to investigate the role of miRNAs in CCL2-mediated MMP expression and cell motility in human osteosarcoma. The results demonstrate a positive correlation between CCL2 or MMP-3 levels and osteosarcoma metastasis, and stimulation of osteosarcoma cells with CCL2 enhances migration and invasion abilities. Additionally, CCL2 enhances MMP-3-dependent cell motility by inhibiting miR-3659 synthesis.
Article
Oncology
Hyun-Ju An, Misun Park, Joon Kim, Young-Hoon Han
INTERNATIONAL JOURNAL OF ONCOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Jisu Oh, Hyeon Hae Lee, Yunhui Jeong, Siyeong Yoon, Hyun-Ju An, Minjung Baek, Do Kyung Kim, Soonchul Lee
Summary: Inadequate vessel maintenance or growth can lead to ischemia in diseases like myocardial infarction and stroke. Developing new compounds to salvage ischemic tissues is urgent, with drug repurposing and computational virtual screening playing key roles in efficient and cost-effective drug discovery. This study successfully identified ellipticine as a pro-angiogenic substance through virtual computational methods and demonstrated its efficacy at a sublethal dose.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Hyun-Ju An, Kyung Rae Ko, Minjung Baek, Yoonhui Jeong, Hyeon Hae Lee, Hyungkyung Kim, Do Kyung Kim, So-Young Lee, Soonchul Lee
Summary: The study aimed to investigate the therapeutic effects of combinatorial therapy using adipose tissue-derived pericytes and NELL-1 on osteonecrosis. The results showed synergistic effects on pro-angiogenic and osteogenic efficacy, with maximum bone formation observed in the mouse model for osteonecrosis. This combination therapy may have potential as a novel treatment for osteonecrosis.
Article
Biochemistry & Molecular Biology
Jin-Woo Kim, Hyun-Ju An, HyunJeong Yeo, Yunhui Jeong, HyeonHae Lee, Jusung Lee, Kisik Nam, Jongheon Lee, Dong-Eun Shin, Soonchul Lee
Summary: Activation of the HIF-1α signaling pathway can protect intervertebral discs from degeneration and may serve as a potential therapeutic target in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
So-Young Lee, Hyun-Ju An, Jin Man Kim, Min-Ji Sung, Do Kyung Kim, Hyung Kyung Kim, Jongbeom Oh, Hye Yun Jeong, Yu Ho Lee, Taeyoung Yang, Jun Han Kim, Ha Jeong Lim, Soonchul Lee
Summary: PINK1 is critical for mitochondrial quality control and decreasing reactive oxygen species production during osteoblast differentiation. Inhibition of PINK1 leads to impaired osteoblast differentiation and defects in mitochondrial homeostasis, while activation of PINK1 may be a potential treatment target for bone diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cardiac & Cardiovascular Systems
Kyeong Mi Kim, Hyun-Ju An, Sang-Hoon Kim, JuHee Kim, Changgon Sim, Jaemin Lee, Sin Hyung Park, Hyun Il Lee, Inseok Jang, Soonchul Lee
Summary: This study aimed to investigate the angiogenic potential of pericytes in diabetes-induced wounds. The results showed that pericytes enhanced vascularization and promoted wound healing. Therefore, pericyte transplantation may be a suitable treatment option for ischemic foot ulcers.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Orthopedics
Kyung Rae Ko, Soo-Hong Han, Sujin Choi, Hyun-Ju An, Eun-Bee Kwak, Yunhui Jeong, Minjung Baek, Jusung Lee, Junwon Choi, Il-Su Kim, Soonchul Lee
Summary: This study investigates the therapeutic effects of substance P inhibitor (SPI) on inflamed tenocytes in vitro and in a collagenase-induced rat model of tendinopathy in vivo. The results showed that SPI improved tendon healing and restoration in the rat model.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Article
Oncology
Sujin Choi, Soonchul Lee, Young-Hoon Han, Junwon Choi, Isaac Kim, Jusung Lee, Hyun-Ju An
Summary: miR-31-3p is expressed at lower levels in prostate cancer tissues compared to adjacent normal tissues, and it plays a tumor-suppressive role by inducing apoptosis and decreasing cell proliferation, migration, invasiveness. GABBR2 is identified as a direct target of miR-31-3p.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Jisun Oh, Hyun-Ju An, Hyun Jeong Yeo, Sujin Choi, Jisu Oh, Segi Kim, Jin Man Kim, Junwon Choi, Soonchul Lee
Summary: This study suggests that colchicine may have therapeutic potential in osteosarcoma by regulating cell cycle signaling to inhibit the growth and spread of osteosarcoma cells.
FRONTIERS IN ONCOLOGY
(2022)
Article
Medicine, General & Internal
Hyun-Ju An, Jae-Hwa Kim, Siyeong Yoon, Junwon Choi, Jeongmo Koo, Soonchul Lee
Summary: This study aimed to identify specific genes or genetic variants associated with rotator cuff tears through a genome-wide association study. More than 20 significantly associated SNPs were found. However, the study was limited by the small sample size and requires further validation. Nevertheless, the identification of potential genetic variants related to rotator cuff injury would aid in the early detection of individuals at risk and provide insight into future gene therapies.
Article
Cell Biology
Min Heui Ha, Man S. S. Kim, Hyun-Ju An, Min-Ji Sung, Yu Ho Lee, Dong-Ho Yang, Sang Hyun Jung, Jihyun Baek, Yueun Choi, Deanne M. Taylor, Yuanchao Zhang, So-Young Lee, Hye Yun Jeong
Summary: Mitochondrial dysfunction is a key factor in the aging process of chronic kidney disease, particularly through the cGAS-STING pathway. PINK1 deficiency contributes to kidney fibrosis, injury, and cellular senescence, and activates the cGAS-STING pathway. Mitochondrial metabolic dysregulation caused by PINK1 deficiency is associated with renal aging.
Article
Urology & Nephrology
Hye Yun Jeong, Hyun-Ju An, Min Ji Sung, Min Heui Ha, Yu Ho Lee, Dong Ho Yang, Tae Young Yang, Dohyun Han, So-Young Lee
Summary: The prevalence of chronic kidney disease (CKD) is increasing and it is a global health burden. This study investigated the change in physical performance after exercise in dialysis-dependent patients with CKD and analyzed differentially expressed proteins before and after the exercise. The results showed that exercise improved physical performance and the significantly expressed proteins were involved in immune response.
Article
Endocrinology & Metabolism
Dong Eun Shin, Younghun Lee, Hyun-Ju An, Tae-Sun Hwang, Jin-Woo Cho, Jongbeom Oh, Wooyeol Ahn, Jaemin Lee, Chul Gie Hong, Yeonju Lee, Soonchul Lee
Summary: Through cadaveric and clinical studies, it was found that the superior trabecular structure of lumbar vertebral bodies is more prone to causing osteoporotic vertebral fractures compared to the inferior trabecular structure. Physicians should focus on the bone mineral density values of the superior region when evaluating osteoporosis.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Oncology
Sujin Choi, Kyeongmi Kim, Hyunjeong Yeo, Gyurim Lee, Isaac Kim, Jisu Oh, Hyun-Ju An, Soonchul Lee
Summary: miRNAs play a critical role in the pathogenesis and progression of tumors. However, the specific role of miR-4284 in renal cell carcinoma (RCC) remains poorly understood. This study investigated the tumor-suppressive role of miR-4284 in renal cancer cell lines. The results showed that overexpression of miR-4284 inhibited proliferation, induced apoptosis, and suppressed tumorigenic characteristics in renal cancer cells. Furthermore, miR-4284 directly targeted GAD1 gene, and xenograft experiments confirmed the tumor-growth-suppressive function of miR-4284 in vivo. Overall, these findings highlight the potential of miR-4284 as a therapeutic target for anti-cancer miRNA therapy in RCC.
Article
Medicine, General & Internal
Jun Mo Kang, Hyun-Seob Lee, Junghyun Kim, Dong Ho Yang, Hye Yun Jeong, Yu Ho Lee, Dong-Jin Kim, Seon Hwa Park, MinJi Sung, Jaehee Kim, Hyun-Ju An, Sang Ho Lee, So-Young Lee
JOURNAL OF KOREAN MEDICAL SCIENCE
(2020)