Review
Biochemistry & Molecular Biology
Pieter Jan Koopmans, Ahmed Ismaeel, Katarzyna Goljanek-Whysall, Kevin A. Murach
Summary: This article provides evidence for the role of miRNAs in controlling satellite cell function in adult skeletal muscle. It emphasizes the indispensability of miRNAs for satellite cell viability and the phases of myogenesis. The interplay between miRNAs and myogenic cell redox status, senescence, and communication to other muscle-resident cells during muscle adaptation is also discussed. Recent satellite cell miRNA profiling studies are summarized, and the need for novel genetic tools to further investigate miRNA control of satellite cell behavior in adult skeletal muscle in vivo is identified.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Genetics & Heredity
Carlos Palma-Flores, Paola B. Zarate-Segura, J. Manuel Hernandez-Hernandez, Sergio de los Santos, Andrea S. Tejeda-Gomez, Luis Javier Cano-Martinez, Patricia Canto, Jorge Omar Garcia-Rebollar, Ramon M. Coral-Vazquez
Summary: This study demonstrates that (-)-epicatechin upregulates miRNAs involved in muscle adaptation, induces muscle hypertrophy, and increases the level of myogenic proteins.
Article
Biochemistry & Molecular Biology
Mengxun Li, Quan Liu, Su Xie, Chong Fu, Jiaxuan Li, Cheng Tian, Xin Li, Changchun Li
Summary: In this study, it was found that the lncRNA TCONS_00323213 is upregulated during porcine skeletal muscle satellite cell (PSC) differentiation and plays a role in the proliferation and differentiation of PSC. The interaction between TCONS_00323213 and PKNOX2 promotes the expression of MyoG and enhances PSC differentiation. This finding provides insights into the molecular mechanisms by which lncRNAs regulate porcine myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Zhenzhen Xiong, Mengni Wang, Shanshan You, Xiaoyan Chen, Jiangguo Lin, Jianhua Wu, Xiaozhong Shi
Summary: In this study, we identified the mechanism regulating the dynamic expression of Tceal7 during skeletal muscle regeneration, and demonstrated the interaction between the triple complex of Mef2c, Creb1, and Myod and the Mef2#3-CRE#3-E#4 motifs in the Tceal7 promoter, which drives Tceal7 expression.
Article
Biochemistry & Molecular Biology
Chrystalla Mytidou, Andrie Koutsoulidou, Anna Katsioloudi, Marianna Prokopi, Konstantinos Kapnisis, Kyriaki Michailidou, Andreas Anayiotos, Leonidas A. Phylactou
Summary: Exosomes are extracellular vesicles released from cells, mediating cell-to-cell and tissue-to-tissue communication in normal and pathological conditions. Muscle tissues secrete exosomes containing specific miRNAs, which vary in sorting between skeletal muscles of different fiber types, influencing local and systemic communication between muscles.
Article
Biochemistry & Molecular Biology
Cynthia M. McKee, Douglas J. Chapski, Michelle Wehling-Henricks, Manuel Rosa-Garrido, Makoto Kuro-O, Thomas M. Vondriska, James G. Tidball
Summary: This study reveals that modulation of muscle stem cell numbers during early postnatal development has long-term effects on muscle growth. The anti-aging protein Klotho influences myogenesis by increasing satellite cell numbers and influencing the epigenetic regulation of genes involved in myogenesis. Klotho reduces the expression of Jmjd3, leading to decreased expression of Wnt genes and inhibition of canonical Wnt signaling, which delays muscle differentiation.
Review
Biochemistry & Molecular Biology
Rachel J. Waldemer-Streyer, Dongwook Kim, Jie Chen
Summary: Regeneration of adult skeletal muscle in mammals is a complex process regulated by multiple proteins and signaling pathways. Cytokines play a crucial role in skeletal myogenesis, with both infiltrating immune cells and the muscle itself secreting these signaling molecules. This review focuses on muscle-secreted cytokines that have been shown to be involved in myogenic differentiation and muscle regeneration.
Review
Biochemistry & Molecular Biology
Cecilia Romagnoli, Teresa Iantomasi, Maria Luisa Brandi
Summary: Skeletal muscle, accounting for 40% of total adult human body mass, is crucial for posture, locomotion, and breathing. Muscle wasting due to aging and diseases can result in loss of muscle mass and functionality, associated with dysfunction of satellite cells. The need for human models in studying skeletal muscle biology has led to the development of in vitro 2D/3D models for pre-clinical investigations and future directions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Silvia Pomella, Sara G. Danielli, Rita Alaggio, Willemijn B. Breunis, Ebrahem Hamed, Joanna Selfe, Marco Wachtel, Zoe S. Walters, Beat W. Schaefer, Rossella Rota, Janet M. Shipley, Simone Hettmer
Summary: Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma in children and adolescents, characterized by aberrant muscle differentiation. Abnormalities in the regulatory transcription factors (MRFs) involved in skeletal muscle development contribute to tumorigenesis in RMS. Core regulatory circuitries (CRCs) and hierarchically organized subsets of cells play a role in maintaining the disease-driving fusion oncogene and promoting malignancy in RMS. Understanding the genetic and epigenetic framework of abnormal muscle differentiation in RMS provides insights into its mechanisms and potential therapeutic strategies.
Review
Physiology
Manoel L. Costa, Arnon D. Jurberg, Claudia Mermelstein
Summary: The study of skeletal muscle fiber development has been ongoing for 70 years, with the use of various animal models such as rats, mice, fruit flies, chickens, and quails. The chick primary muscle culture model provides a robust and advantageous approach to studying skeletal myogenesis, allowing for detailed analysis of myoblast proliferation, differentiation, and formation of multinucleated myotubes. This model demonstrates high levels of differentiation independent of serum withdrawal, and is expected to foster new studies on muscle proliferation and differentiation in conditions more similar to in vivo situations than muscle cell lines.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Li Li, Chenyu Qin, Yuan Chen, Wei Zhao, Qi Zhu, Dinghui Dai, Siyuan Zhan, Jiazhong Guo, Tao Zhong, Linjie Wang, Jiaxue Cao, Hongping Zhang
Summary: The interplay between coding and noncoding factors is crucial for muscle growth and development. This study reveals a mutual activation between the long noncoding RNA H19 and MyoD, and describes the regulatory mechanisms involved.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Cecilia Romagnoli, Roberto Zonefrati, Elena Lucattelli, Marco Innocenti, Roberto Civinini, Teresa Iantomasi, Maria Luisa Brandi
Summary: This study evaluated the effects of PTH on the proliferation and differentiation of skeletal muscle satellite cells isolated from human biopsies. Results showed no significant changes in cAMP and proliferation in the isolated cells treated with PTH, but differentiated myotubes treated with PTH exhibited increased cAMP, expression of myogenic differentiation genes, and MHC protein. This study provides new insights into the effects of PTH on human skeletal muscle cells and opens new avenues for research in muscle pathophysiology.
Review
Physiology
Peter O. Isesele, Vera C. Mazurak
Summary: Skeletal muscle is primarily composed of myofibers, with their size and muscle mass being influenced by various pathological and physiological conditions. Satellite cells play a crucial role in muscle regeneration, but a reduced number and impaired myogenic function may lead to muscle disorders. The function of satellite cells is affected by mitochondrial function, inflammation, and Omega-3 PUFAs.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell & Tissue Engineering
Luca Dalle Carbonare, Gianluigi Dorelli, Veronica Li Vigni, Arianna Minoia, Jessica Bertacco, Samuele Cheri, Michela Deiana, Giulio Innamorati, Mattia Cominacini, Cantor Tarperi, Federico Schena, Monica Mottes, Maria Teresa Valenti
Summary: MicroRNAs play an important role in myogenesis and muscle activity, and can be modulated by physical exercise. A study examined the expression of microRNAs in response to physical performance, and found gender-specific expression patterns before exercise. The levels of certain microRNAs changed after a half marathon in both males and females, and these changes were reflected in the blood. In addition, the serum of marathon runners had a positive effect on myogenesis in cell cultures. These findings suggest that microRNAs could be potential targets for therapies involving myogenic stem cells in diseases.
STEM CELL REVIEWS AND REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Hideaki Fujita, Masanobu Horie, Kazunori Shimizu, Eiji Nagamori
Summary: C2C12 myotubes stimulated by electric pulses are of interest in muscle physiology and biotechnology due to the enhancement of sarcomere structure development and active tension generation capacity by electric pulse stimulation (EPS). However, termination of EPS results in rapid loss of active tension and disassembly of sarcomere structure. Transcriptomic analysis showed upregulation and downregulation of multiple genes after EPS termination, but no genes related to muscle atrophy were found.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Editorial Material
Cell & Tissue Engineering
Yuefeng Li, F. Jeffrey Dilworth
Article
Biochemistry & Molecular Biology
Valentina Casa, Valeria Runfola, Stefano Micheloni, Arif Aziz, F. Jeffrey Dilworth, Davide Gabellini
HUMAN MOLECULAR GENETICS
(2017)
Article
Cell Biology
Mohammad H. Al-Khalaf, Leanne E. Blake, Brian D. Larsen, Ryan A. Bell, Steve Brunette, Robin J. Parks, Michael A. Rudnicki, Peter J. McKinnon, F. Jeffrey Dilworth, Lynn A. Megeney
Article
Cell Biology
Elena N. Fazio, Claire C. Young, Jelena Toma, Michael Levy, Kurt R. Berger, Charis L. Johnson, Rashid Mehmood, Patrick Swan, Alphonse Chu, Sean P. Cregan, F. Jeffrey Dilworth, Christopher J. Howlett, Christopher L. Pin
MOLECULAR BIOLOGY OF THE CELL
(2017)
Editorial Material
Biochemistry & Molecular Biology
Marjorie Brand, F. Jeffrey Dilworth
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Editorial Material
Biochemistry & Molecular Biology
Marjorie Brand, F. Jeffrey Dilworth
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Review
Cell Biology
Kiran Nakka, Claudia Ghigna, Davide Gabellini, F. Jeffrey Dilworth
Article
Biochemistry & Molecular Biology
Meshach Asare-Werehene, Kiran Nakka, Arkadiy Reunov, Chen-Tzu Chiu, Wei-Ting Lee, Mohammad R. Abedini, Pei-Wen Wang, Dar-Bin Shieh, F. Jeffrey Dilworth, Euridice Carmona, Tien Le, Anne-Marie Mes-Masson, Dylan Burger, Benjamin K. Tsang
Article
Cell Biology
Jimmy Massenet, Cyril Gitiaux, Melanie Magnan, Sylvain Cuvellier, Arnaud Hubas, Patrick Nusbaum, F. Jeffrey Dilworth, Isabelle Desguerre, Benedicte Chazaud
Article
Biochemistry & Molecular Biology
Tapan Sharma, Daniel C. L. Robinson, Hanna Witwicka, F. Jeffrey Dilworth, Anthony N. Imbalzano
Summary: Skeletal muscle regeneration is mediated by myoblasts that undergo epigenomic changes to establish the gene expression program of differentiated myofibers. mSWI/SNF chromatin remodeling enzymes coordinate with lineage-determining transcription factors to establish the epigenome of differentiated myofibers. Bromodomains bind to acetylated lysines on histone N-terminal tails and other proteins. The mutually exclusive ATPases of mSWI/SNF complexes, BRG1 and BRM, contain bromodomains with undefined functional importance in skeletal muscle differentiation. Pharmacological inhibition of mSWI/SNF bromodomain function using the small molecule PFI-3 reduced differentiation in cell culture and in vivo through decreased myogenic gene expression, while increasing cell cycle-related gene expression and the number of cells remaining in the cell cycle. Comparative gene expression analysis with data from myoblasts depleted of BRG1 or BRM showed that bromodomain function was required for a subset of BRG1- and BRM-dependent gene expression. Reduced binding of BRG1 and BRM after PFI-3 treatment showed that the bromodomain is required for stable chromatin binding at target gene promoters to alter gene expression. Our findings demonstrate that mSWI/SNF ATPase bromodomains permit stable binding of the mSWI/SNF ATPases to promoters required for cell cycle exit and establishment of muscle-specific gene expression.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Joana Esteves de Lima, Reem Bou Akar, Leo Machado, Yuefeng Li, Bernadette Drayton-Libotte, F. Jeffrey Dilworth, Frederic Relaix
Summary: The authors demonstrate that HIRA, a H3.3 histone chaperone, establishes the chromatin landscape required for skeletal muscle cell identity.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Ryan A. Bell, Mohammad H. Al-Khalaf, Steve Brunette, Dalal Alsowaida, Alphonse Chu, Hina Bandukwala, Georg Dechant, Galina Apostolova, F. Jeffrey Dilworth, Lynn A. Megeney
Summary: This study reveals that the removal of SATB2 protein is a crucial event in initiating myogenic differentiation, which leads to chromatin remodeling and the expression of differentiation-inducing factors while limiting the expression of inhibitory genes.
Article
Multidisciplinary Sciences
Kiran Nakka, Sarah Hachmer, Zeinab Mokhtari, Radmila Kovac, Hina Bandukwala, Clara Bernard, Yuefeng Li, Guojia Xie, Chengyu Liu, Magid Fallahi, Lynn A. Megeney, Julien Gondin, Benedicte Chazaud, Marjorie Brand, Xiaohui Zha, Kai Ge, F. Jeffrey Dilworth
Summary: Inflammatory cytokine signaling from the regenerative niche impairs the ability of quiescent MuSCs to reenter the cell cycle, but JMJD3-driven hyaluronic acid synthesis plays a proregenerative role that allows MuSC adaptation to inflammation and the initiation of muscle repair.
Article
Biochemical Research Methods
Yuefeng Li, Kiran Nakka, Thomas Olender, Philippe Gingras-Gelinas, Matthew Man-Kin Wong, Daniel C. L. Robinson, Hina Bandukwala, Carmen G. Palii, Odile Neyret, Marjorie Brand, Alexandre Blais, F. Jeffrey Dilworth
Summary: The study utilized a new technology to investigate the chromatin landscape and transcription factor binding of muscle stem cells, yielding positive results in both mouse and human stem cells.
Article
Cell Biology
Zhaoyi Chen, Wing Y. Chang, Alton Etheridge, Hilmar Strickfaden, Zhigang Jin, Gareth Palidwor, Ji-Hoon Cho, Kai Wang, Sarah Y. Kwon, Carole Dore, Angela Raymond, Akitsu Hotta, James Ellis, Rita A. Kandel, F. Jeffrey Dilworth, Theodore J. Perkins, Michael J. Hendzel, David J. Galas, William L. Stanford
