期刊
FASEB JOURNAL
卷 29, 期 7, 页码 2970-2979出版社
WILEY
DOI: 10.1096/fj.15-270553
关键词
type 2 diabetes; amylin; pancreatic islets; Erk1/2; Akt
资金
- Intituto de Salud Carlos III
- Ministerio de Economia y Competitividad [PI11/00679, PI14/00447, BFU2010-17639]
- Fondo Europeo de Desarrollo Regional, Union Europea, Una Manera de Hacer Europa
- Centro de Investigacion Biomedica en Red (Proyecto Integrado de Excelencia) [PIE1400061]
- Centro de Investigacion Biomedica en Red de Diabetes y Enfermedades Metabolicas Asociadas
- Institut d'Investigacions Biomediques August Pi i Sunyer Predoctoral Fellowship
- Sarda Farriol Research Programme
The toxic effects of human islet amyloid polypeptide (IAPP) on pancreatic islets have been widely studied. However, much less attention has been paid to the physiologic actions of IAPP on pancreatic beta cells, which secrete this peptide together with insulin upon glucose stimulation. Here, we aimed to explore the signaling pathways and mitogenic actions of IAPP on beta cells. We show that IAPP activated Erk1/2 and v-akt murine thymoma viral oncogene homolog 1 (Akt) at the picomolar range (10-100 pM) in mouse pancreatic islets and MIN6 beta cells cultured at low glucose concentrations. In contrast, IAPP decreased the induction of these pathways by high glucose levels. Consistently, IAPP induced a 1.7-fold increase of beta-cell proliferation at low-glucose conditions, whereas it reduced beta-cell proliferation at high glucose levels. Strikingly, the specific antagonist of the IAPP receptor AC187 (100 nM) decreased the activation of Erk1/2 and Akt and reduced beta-cell proliferation by 24% in glucose-stimulated b cells, uncovering a key role of endogenously released IAPP in beta-cell responses to glucose. We conclude that exogenously added IAPP exerts a dual effect on beta-cell mitogenic signaling and proliferation, depending on the glucose concentration. Importantly, secreted IAPP contributes to the signaling and mitogenic response of beta cells to glucose through an autocrine mechanism.
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