Article
Biotechnology & Applied Microbiology
Wenting Gao, Ying Yin, Pan Wang, Wei Tan, Mingliang He, Jianping Wen
Summary: In this study, the genes of the Dahms xylose-utilization pathway were integrated into Bacillus subtilis and a metabolic control module was designed, resulting in an increased yield of fengycin. Metabonomic analysis revealed the underlying factors for the increase of fengycin titer.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Angelo B. Banares, Grace M. Nisola, Kris Nino G. Valdehuesa, Won-Keun Lee, Wook-Jin Chung
Summary: This review discusses the causes of D-xylonic acid accumulation in metabolically engineered XOP-utilizing microorganisms, and how to address this issue through different engineering and synthetic biology techniques, with a focus on bacterial strains. Additionally, the ability of certain microorganisms to produce and tolerate D-xylonic acid is also examined as an important aspect in developing efficient microbial cell factories.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Cui Zhao, Xi-Hui Wang, Xin-Yao Lu, Hong Zong, Bin Zhuge
Summary: In this study, a transcription factor-mediated ergosterol feedback system was developed to regulate the metabolism of ergosterol and redirect carbon flux to geraniol synthesis. Through stepwise metabolic engineering, a geraniol titer of 531.7 mg L-1 was achieved. Additionally, the xylose assimilation pathway was constructed in Candida glycerinogenes, and the strain's growth was improved by activating the pentose phosphate pathway. This resulted in the production of 1091.6, 862.4, and 921.8 mg L-1 of geraniol from different lignocellulosic biomass sources.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Microbiology
Pengfei Gu, Fangfang Li, Zhaosong Huang
Summary: This study aims to address the depletion of fossil fuels and environmental pollution by utilizing xylose or glucose-xylose mixture as raw materials for microbial fermentation to produce isobutanol. By optimizing and combining different pathways, a synthetic pathway for isobutanol production from xylose was successfully constructed. The engineered microbial strains demonstrated efficient utilization of xylose and high isobutanol production.
Article
Biotechnology & Applied Microbiology
Rhudith B. Cabulong, Angelo B. Banares, Grace M. Nisola, Won-Keun Lee, Wook-Jin Chung
Summary: A modified microbial strain capable of producing glycolic acid from d-xylose was engineered to utilize cellobiose as a co-substrate, resulting in improved glycolic acid production with high yield.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Agriculture, Multidisciplinary
Marcelo R. A. De Figueiredo, Hamlin Barnes, Claudia M. Boot, Ana Beatriz T. B. De Figueiredo, Scott J. Nissen, Franck E. Dayan, Todd A. Gaines
Summary: Amaranthus tuberculatus, a waterhemp plant species resistant to 2,4-dichlorophenoxyacetic acid (2,4-D), has been found to possess rapid metabolic detoxification ability. Through a novel detoxification mechanism, resistant plants hydroxylate 2,4-D into a less toxic metabolite, while susceptible plants conjugate 2,4-D to aspartate. This evolution of detoxification reactions in resistant plants allows for more efficient detoxification of 2,4-D.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Yixuan Zhu, Jingtao Zhang, Lang Zhu, Zefang Jia, Qi Li, Wei Xiao, Limin Cao
Summary: Efficient xylose fermentation in budding yeast was achieved through rational promoter elements engineering, with HXT7 showing the best performance among surveyed promoters. The redox balance of the xylose utilization pathway was optimized to achieve a balanced xylose metabolism toward ethanol formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jiwon Kim, Sungmin Hwang, Sun-Mi Lee
Summary: This review discusses the metabolic engineering efforts to produce carbon-neutral fuels and chemicals from lignocellulosic biomass, and the application of systems biology in accelerating the development of microbial cell factories.
METABOLIC ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Izabela Szymczak-Pajor, Krystian Miazek, Anna Selmi, Aneta Balcerczyk, Agnieszka Sliwinska
Summary: Adipose tissue plays a crucial role in metabolism, while deficiency in vitamin D can disrupt its functioning. Restoring the proper functionality of adipose tissue is important in reducing the risk of obesity-related complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Dongdong Zhao, Xinna Zhu, Hang Zhou, Naxin Sun, Ting Wang, Changhao Bi, Xueli Zhang
Summary: Effective metabolic pathways are crucial for efficient cell factories, and utilizing CRISPR technology in pathway engineering can enhance efficiency, reduce costs, and simplify target customization. This technology has been proven to be robust and effective in various organisms.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Zhou Deng, Yinghui Mu, Zhi Chen, Lishi Yan, Xin Ju, Liangzhi Li
Summary: This study demonstrates the potential of T. oedocephalis in the synthesis of multiple useful products including erythritol from xylose.
BIOTECHNOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Theodora Panagaki, Laszlo Pecze, Elisa B. Randi, Anni I. Nieminen, Csaba Szabo
Summary: Increased H2S generation in Down syndrome (DS) promotes pseudohypoxia and contributes to cellular metabolic dysfunction by causing a shift from oxidative phosphorylation to glycolysis.
Review
Environmental Sciences
Yihui Ruan, Xin Fang, Tingyue Guo, Yiting Liu, Yu Hu, Xuening Wang, Yuxin Hu, Lanyue Gao, Yongfang Li, Jingbo Pi, Yuanyuan Xu
Summary: Chronic exposure to arsenic is associated with various cancers, with metabolic reprogramming being a key mechanism. Research has primarily focused on glucose reprogramming induced by arsenic exposure. Apart from enhanced glycolysis, arsenic may also disrupt lipid deposition and induce one-carbon metabolism pathway.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Review
Biochemistry & Molecular Biology
Medhi Wangpaichitr, George Theodoropoulos, Dan J. M. Nguyen, Chunjing Wu, Sydney A. Spector, Lynn G. Feun, Niramol Savaraj
Summary: The development of drug resistance in tumors is a significant complication to cancer chemotherapy, but targeting cisplatin-resistant tumors with new combination therapy may offer a major improvement. Recent research suggests that some tumors switch from glycolysis to oxidative metabolism, which is crucial for treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Fengyu Yang, Kangling Qiu, Yichun Zhu, Xian Zhang, Taowei Yang, Ganfeng Yi, Meijuan Xu, Zhiming Rao
Summary: In this study, metabolic engineering strategies were designed to significantly enhance putrescine production in Corynebacterium crenatum SYPA. By optimizing the synthetic pathway, regulating gene expression, blocking degradation pathways, and constructing a xylose utilization pathway, the titer of putrescine was greatly increased. The engineered strains also showed the potential to produce putrescine from biomass hydrolysates.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Yuqing Yang, Tianqi Wang, Qiaoli Yu, Huaiwei Liu, Luying Xun, Yongzhen Xia
Summary: The recombination of short homologous ends in Escherichia coli involves the digestion of linear dsDNA by exonucleases ExoIII and ExoX, followed by annealing and extension of single-stranded overhangs, ultimately joined by Ligase A. This model, requiring Pol I polymerase activity, differs from established recombination pathways and is likely common in bacteria.
MOLECULAR MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Angelo B. Banares, Grace M. Nisola, Kris N. G. Valdehuesa, Won-Keun Lee, Wook-Jin Chung
Summary: The lignocellulosic sugar d-xylose is gaining attention as a low-cost alternative substrate for genetically engineered organisms. Escherichia coli is widely used as the host organism for engineered microbial cell factories, with a deep understanding of its systems. Limited literature reviews focus on using engineered E. coli for converting d-xylose to target compounds, making this review essential for researchers in this field.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tianqi Wang, Mingxue Ran, Xiaoju Li, Yequn Liu, Yufeng Xin, Honglei Liu, Huaiwei Liu, Yongzhen Xia, Luying Xun
Summary: Our study provides evidence of H2S oxidation producing octasulfur globules via sulfide:quinone oxidoreductase (SQR) catalysis and spontaneous reactions in the bacterial cytoplasm. Understanding this process is important for geochemical cycling and supports the use of heterotrophic bacteria with SQR to oxidize toxic H2S into recoverable sulfur globules.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Ting Lu, Xiaohua Wu, Qun Cao, Yongzhen Xia, Luying Xun, Huaiwei Liu
Summary: This study reveals a new posttranslational regulation mechanism involving sulfane sulfur that stimulates antibiotic production in Streptomyces, providing insights into the regulation of biosynthetic gene clusters in this genus.
Article
Microbiology
Yuqing Yang, Qiaoli Yu, Min Wang, Rui Zhao, Huaiwei Liu, Luying Xun, Yongzhen Xia
Summary: This study found that using E.coli BW25113 (recA(+)) competent cells can achieve higher transformation efficiency and cloning success rate compared to commonly used E.coli cloning strains, with RecA playing a partial role in the differences. Even with the recA deletion in BW25113, the transformation efficiency and cloning success rate are still significantly higher than those of commonly used cloning strains. These findings suggest that E.coli BW25113 is an ideal choice for efficient cloning and DNA assembly.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Rui Zhao, Zimai Li, Yuqing Sun, Wei Ge, Mingyu Wang, Huaiwei Liu, Luying Xun, Yongzhen Xia
Summary: Hyperuricemia, the second most prevalent metabolic disease, can be effectively treated by introducing recombinant urate-degrading bacteria to enhance intestinal secretion, according to a study.
Article
Biochemistry & Molecular Biology
Tianqi Wang, Yuqing Yang, Menghui Liu, Honglei Liu, Huaiwei Liu, Yongzhen Xia, Luying Xun
Summary: Elemental sulfur serves as a strong oxidant, entering yeast cells and reacting with glutathione to produce glutathione persulfide, H2S, and glutathione disulfide, leading to inhibited oxygen consumption and increased reactive oxygen species, ultimately inhibiting yeast growth.
Article
Environmental Sciences
John Edward L. Sio, Erwin C. Escobar, Hern Kim, Wook-Jin Chung, Grace M. Nisola
Summary: A magnetic adsorbent HPCA@SiO2@Fe3O4 with high HPCA loading and good stability was developed for Co (II) recovery from liquid waste.
Article
Microbiology
Daixi Liu, Jinyu Chen, Yafei Wang, Yue Meng, Yuanning Li, Ranran Huang, Yongzhen Xia, Huaiwei Liu, Nianzhi Jiao, Luying Xun, Jihua Liu
Summary: Cyanobacteria use peroxiredoxin to reduce high levels of sulfane sulfur, possibly the original role of peroxiredoxin. The reduction of sulfane sulfur by peroxiredoxin may offer cyanobacteria an advantage in the complex environment.
Article
Biochemistry & Molecular Biology
Xiaohua Wu, Yuping Xin, Qingda Wang, Yongzhen Xia, Luying Xun, Huaiwei Liu
Summary: The use of alkyl halides to tag reactive sulfur species faces a challenge as RSS can also initiate a reductive dehalogenation reaction, generating reduced tag and oxidized RSS. This may lead to underestimation of RSS content in bio-samples and inaccuracies in determining its species when using alkyl halide agents for analysis. Further studies are needed to quantify the extent of this underestimation.
Article
Biochemistry & Molecular Biology
Qiaoli Yu, Mingxue Ran, Yuqing Yang, Huaiwei Liu, Luying Xun, Yongzhen Xia
Summary: Sulfane sulfur is a class of compounds containing zero-valent sulfur, which play important signaling roles. This study optimized a method to measure intracellular sulfane sulfur (iSS) levels and identified factors that can influence iSS in Escherichia coli.
Article
Biochemistry & Molecular Biology
Huangwei Xu, Guanhua Xuan, Huaiwei Liu, Honglei Liu, Yongzhen Xia, Luying Xun
Summary: Sulfane sulfur, a signaling molecule in bacteria, regulates the expression of antibiotic resistance genes and stress response genes through MarR-type regulators. The study found that OhrR, a MarR-type regulator in Pseudomonas aeruginosa, also responds to sulfane sulfur, reducing its DNA binding affinity. These findings suggest that sulfane sulfur may be a common inducer for MarR-type regulators, allowing bacteria to resist stress without direct exposure to it.
Article
Microbiology
Yuqing Yang, Menghui Liu, Tianqi Wang, Qian Wang, Huaiwei Liu, Luying Xun, Yongzhen Xia
Summary: DNA cloning requires efficient transformation, which can be achieved using the BW3KD strain and the TSS-HI method for competent cell preparation. The transformation efficiency of BW3KD cells prepared by this method exceeds that of commercial and homemade competent cells. Additionally, these competent cells are effective for transforming large plasmids and assembling multiple DNA fragments.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Ting Lu, Qingda Wang, Qun Cao, Yongzhen Xia, Huaiwei Liu, Luying Xun
Summary: Reactive sulfane sulfur (RSS) has important physiological functions in bacteria, but is toxic at excessive levels. AdpA, a multi-functional transcriptional regulator, is essential for the survival of Streptomyces when facing external RSS stress. This study found that AdpA activates the expression of thioredoxins and thioredoxin reductases, which reduce RSS to gaseous hydrogen sulfide (H2S) in the presence of excess RSS. This discovery unveils a new mechanism of anti-RSS stress in Streptomyces through AdpA and the thioredoxin system.
Article
Biology
Chao Tang, Jingjing Li, Yuemeng Shen, Menghui Liu, Honglei Liu, Huaiwei Liu, Luying Xun, Yongzhen Xia
Summary: Bacillus licheniformis MW3 has the ability to degrade bird feathers, which are rich in hazardous sulfur compounds. The bacterium has a gene cluster that helps it detoxify these compounds. The cluster contains 11 genes, including a repressor gene, two regulatory genes, and two genes that encode enzymes for sulfur oxidation. Together, these genes enable the bacterium to grow on feather and highlight the importance of removing sulfide and sulfane sulfur during feather degradation.
COMMUNICATIONS BIOLOGY
(2023)
