Article
Biotechnology & Applied Microbiology
Jingyan Zhang, Ying Sun, Yeji Wang, Xin Chen, Lu Xue, Jingjing Zhang, Xiangcheng Zhu, Yanwen Duan, Xiaohui Yan
Summary: Rubiginones are part of the angucycline family and have been shown to enhance the cytotoxicity of vincristine against drug-resistant cancer cell lines. Through genome mining of Streptomyces sp. CB02414, new rubiginones were discovered and a biosynthetic pathway was proposed based on the production profiles of wild-type and mutant strains. This research paves the way for further studies on cytochrome P450-catalyzed oxidation of angucyclines and the development of novel compounds through combinatorial biosynthesis strategies.
MICROBIAL CELL FACTORIES
(2021)
Article
Multidisciplinary Sciences
Liyan Yang, Dengfeng Yang, Qingyan Wang, Juan Li, Hong-Liang Li, Lixia Pan
Summary: In this study, the expression and purification of the DoxA enzyme, along with its associated cofactors, were characterized. These findings provide a method for engineering Streptomyces P450s enzymes for mechanistic and structural studies.
Article
Chemistry, Multidisciplinary
Pakjira Nanudorn, Sirinthra Thiengmag, Friederike Biermann, Pelin Erkoc, Sabrina D. Dirnberger, Thao N. Phan, Robert Fuerst, Reiko Ueoka, Eric J. N. Helfrich
Summary: This study discovered a new family of ribosomally synthesized and posttranslationally modified peptides, named atropopeptides. The characterization of two atropopeptide biosynthetic pathways revealed a two-step maturation process. Atropopeptides promote pro-angiogenic cell functions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Bei-Bei He, Jing Liu, Zhuo Cheng, Runze Liu, Zheng Zhong, Ying Gao, Hongyan Liu, Zhi-Man Song, Yongqi Tian, Yong-Xin Li
Summary: Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a class of natural products with diverse structural features and bioactivities. P450-catalyzed RiPPs represent a unique but underexplored family. This study introduces a rule-based genome mining strategy to identify diverse P450-catalyzed RiPPs, highlighting the potential of P450s to expand the chemical diversity of strained macrocyclic peptides and the range of biocatalytic tools available for peptide macrocyclization.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Sebastian Adam, Dazhong Zheng, Andreas Klein, Carsten Volz, William Mullen, Sally L. Shirran, Brian O. Smith, Olga V. Kalinina, Rolf Mueller, Jesko Koehnke
Summary: In this study, we identified three proteins (CgnB, CgnC, and CgnE) responsible for the production of the tetracyclic crocagin core. Crystal structures of two homologues (CgnB and CgnE) provided insights into their functions. We also discovered the roles of CgnD and CgnL in the liberation and modification of the crocagin core, respectively, and proposed a biosynthetic scheme. Bioinformatic analyses further revealed potential pathways for the synthesis of structurally diverse peptide-derived pyrroloindoline alkaloids.
Article
Biotechnology & Applied Microbiology
Petra Pivk Lukancic, Tjasa Drcar, Robert Bruccoleri, Martin Crnugelj, Peter Mrak
Summary: In this study, a new genotype of Streptomyces clavuligerus with the ability to produce teleocidin was discovered. Teleocidin is a highly toxic secondary metabolite. The genome sequence of this genotype provides a foundation for further research and application.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Yangle Gao, Mengsi Xie, Cui Yu, Mingliang Zhang, Jianzhong Huang, Qin Li, Huaidong Zhang, Li Li
Summary: Four new natural products, macrollins A-D, were discovered from the pathogenic fungus Macrophomina phaseolina by heterologous expression, representing the first reported polyketide-amino acid hybrids from a plant pathogen. A new cytochrome P450 mono-oxygenase, MacC, was identified to catalyze hydroxylation at the beta-carbon of tetramic acid molecules, different from other P450s involved in fungal 2-pyridones biosynthesis. Phylogenetic analysis revealed that MacC did not belong to any known clades, and putative oxidative mechanisms of P450s and biosynthetic pathway of macrollins were proposed.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Microbiology
Nsikelelo Allison Malinga, Nomfundo Nzuza, Tiara Padayachee, Puleng Rosinah Syed, Rajshekhar Karpoormath, Dominik Gront, David R. Nelson, Khajamohiddin Syed
Summary: Cytochrome P450 monooxygenases in Salinispora species show high abundance and diversity, with some P450s participating in the biosynthesis of secondary metabolites, suggesting the potential for discovering novel secondary metabolites.
Article
Plant Sciences
Xinyun Cui, Fanqi Meng, Xian Pan, Xiaoxiao Qiu, Sixuan Zhang, Caili Li, Shanfa Lu
Summary: This study reports a high-quality genome assembly of Aristolochia contorta and provides a solid foundation for further elucidation of aristolochic acid biosynthesis and regulation, as well as the molecular breeding of Aristolochiaceae medicinal materials.
HORTICULTURE RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Kanami Watanabe, Mitsuki Kondo, Yoshinori Ikenaka, Shouta M. M. Nakayama, Mayumi Ishizuka
Summary: Our study compares genomic data from 12 mammalian species and investigates a specific genetic loss and duplication in elephants. We found that elephant CYP1-4 genes have undergone independent duplication, particularly in the CYP2A, CYP2C, and CYP3A genes, forming a unique cluster. However, CYP2E and CYP4A in elephants have decayed to a state of genetic dysfunction. These findings provide new insights into elephant xenobiotic metabolism.
Article
Infectious Diseases
Balasubramanian Cibichakravarthy, Polapass Arul Jose
Summary: Streptomyces are a rich source of structurally diverse microbial natural products, with the potential to produce numerous novel secondary metabolites. However, there is currently a mismatch between the speed of genome analysis and the discovery of new compounds.
Article
Biochemistry & Molecular Biology
Xing Tian, Xinxin Su, Chenjing Li, Yifei Zhou, Shuying Li, Jiamin Guo, Qiqi Fan, Shumin Lu, Yalin Zhang
Summary: The presence of cantharidin in blister beetles serves as an important ecological adaptive mechanism against predators and in the regulation of courtship and mating behaviors. By assembling the high-quality genome of Epicauta chinensis and annotating key genes, researchers identified gene expansions associated with environmental adaptation and identified potential candidate genes for cantharidin biosynthesis. The genome also provided insights into unique immune gene evolution in Coleopteran insects and laid the foundation for further studies on blister beetles' development and evolution.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Microbiology
Khorshed Alam, Arpita Mazumder, Suranjana Sikdar, Yi-Ming Zhao, Jinfang Hao, Chaoyi Song, Yanyan Wang, Rajib Sarkar, Saiful Islam, Youming Zhang, Aiying Li
Summary: Natural products derived from Streptomyces play a crucial role in the treatment of diseases and agriculture, yet many remain unexplored. The development of novel antibiotics, agrochemicals, and anticancer medicines is imperative due to increasing resistance. Genomic-driven drug discovery strategies offer a promising future for drug development from Streptomyces.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Wadzani Palnam Dauda, Daji Morumda, Peter Abraham, Charles Oluwaseun Adetunji, Shakira Ghazanfar, Elkanah Glen, Shittu Emmanuel Abraham, Grace Wabba Peter, Israel Ogwuche Ogra, Ulasi Joseph Ifeanyi, Hannatu Musa, Mawuli Kwamla Azameti, Bilal Ahamad Paray, Aneela Gulnaz
Summary: This study investigates the diversity, abundance, evolutionary relationships, and cellular localization of 372 cytochrome P450 in 13 Alternaria species. It reveals the expansion of cytochrome P450s in these fungal genera and provides insights into their biology and toxigenic potentials.
Article
Chemistry, Organic
Run-Zhou Liu, Shanchong Chen, Lihan Zhang
Summary: In this study, we report the discovery of three new isoflavone dimers from Streptomyces cattleya and the identification of a cytochrome P450 monooxygenase, CYP158C1, which can catalyze the dimerization of isoflavones. CYP158C1 was also found to be capable of dimerizing plant-derived polyketides, such as flavonoids and stilbenes, providing new insights into P450-mediated biaryl coupling reactions in biosynthesis.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Nguyen Huy Thuan, Anil Shrestha, Nguyen Thanh Trung, Vinay Bharadwaj Tatipamula, Duong Van Cuong, Nguyen Xuan Canh, Nguyen Van Giang, Tae-Su Kim, Jae Kyung Sohng, Dipesh Dhakal
Summary: Taxifolin and its derivatives are medicinally important flavanonols that are widely distributed in plants and can be synthesized through plant- and microbial-based platforms. These compounds are mainly biosynthesized via the phenylpropanoid pathway, and the detailed investigation of their biosynthesis has provided the foundation for the reconstruction of relevant biosynthetic pathways.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Review
Plant Sciences
Luan Luong Chu, Hanhong Bae
Summary: Bacterial endophytes have a significant impact on the growth and protection of ginseng, with potential applications in the production of food and medicine. Metabolic engineering techniques are accelerating the use of bacterial endophytes in producing beneficial compounds.
JOURNAL OF GINSENG RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ramesh Prasad Pandey, Dipesh Dhakal, Samir Bahadur Thapa, Puspalata Bashyal, Tae-Su Kim, Jae Kyung Sohng
Summary: Epothilone A was biotransformed into three metabolites by Nocardia sp. and recombinant Nocardia overexpressing a specific cytochrome P450, resulting in two hydroxylated derivatives (M1 and M2) and deoxyepothilone A (M3). These newly produced metabolites are expected to exhibit novel cytotoxic effects and may be further studied in pharmacological research.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Luan Luong Chu
Summary: Terpenoids, a group of secondary metabolites with diverse structures and functions, have various applications in human life. Engineering microbial cells using the CRISPR-Cas system offers a sustainable approach to produce terpenoids with high yields. This review summarizes the application of CRISPR-Cas system in terpenoid production and discusses its prospects.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Editorial Material
Microbiology
Dipesh Dhakal, Eung-Soo Kim, Mattheos Koffas
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nguyen Huy Thuan, Haritha Polimati, Ramesh Alluri, Vinay Bharadwaj Tatipamula
Summary: The present study provided scientific evidence for the traditional usage of Cladonia pyxidata in treating tuberculosis (TB). The antimycobacterial activity of isolated metabolites from C. pyxidata was evaluated against different strains, and usnic acid and fumarprotocetraric acid were found to be the key active compounds. These compounds showed more effective inhibition against multi-drug resistant strains compared to the first-line drug rifampicin.
Review
Biotechnology & Applied Microbiology
Nguyen Huy Thuan, Vinay Bharadwaj Tatipamula, Nguyen Xuan Canh, Nguyen Van Giang
Summary: Microbial co-culture is an effective method to increase the production of target compounds by simplifying the design of biosynthetic pathways and restricting the metabolic burden of the host. Different strategies can be used to overcome metabolic burdens and establish autonomous modulation of cell growth.
Article
Biotechnology & Applied Microbiology
Purna Bahadur Poudel, Ramesh Prasad Pandey, Dipesh Dhakal, Tae-Su Kim, Trang Thi Huyen Nguyen, Hye Jin Jung, Hee Jeong Shin, Binod Timalsina, Jae Kyung Sohng
Summary: In this study, a sugar-O-methyltransferase ThnM1 was identified and functionally characterized. The enzyme showed promiscuity and regiospecific activity at the 2'-OH of rhamnose. By using engineered Escherichia coli, a methoxy-rhamnosyl derivative of quinizarin was successfully produced. The cytotoxicity of the derivative was studied and compared with that of quinizarin. This study provides insights into the potential diversification of natural products through the methylation of the rhamnose moiety.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Rubin Thapa Magar, Van Thuy Thi Pham, Purna Bahadur Poudel, Hue Thi Nguyen, Adzemye Fovennso Bridget, Jae Kyung Sohng
Summary: In this study, a new macrolide called 25-hydroxy peucemycin was successfully identified from Streptomyces peucetius ATCC 27,952, which exhibited antibacterial effects on certain pathogens. By inactivating a polyketide synthase gene, the biosynthetic gene cluster for the compound was identified, which is considered to be cryptic. The biosynthetic pathway of peucemycin involves five type I polyketide synthase genes encoding eight modules and utilizes unusual extender units.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Adzemye Fovennso Bridget, Chung Thanh Nguyen, Rubin Thapa Magar, Jae Kyung Sohng
Summary: In this study, a vector was designed and constructed to introduce foreign genes into the chromosome of S. spinosa, resulting in a significant increase in spinosad production.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Luan Luong Chu, Nguyen Quang Huy, Nguyen Huu Tung
Summary: Ginsenosides are bioactive compounds found in the Panax species with various pharmaceutical properties. Microbial production, compared to plant extraction, offers advantages such as high efficiency and selectivity. This review summarizes the cell biological mechanisms of anticancer activities and current research on ginsenoside production, highlighting the use of microbial hosts as a promising approach.
Article
Biochemistry & Molecular Biology
Su Yeong Ma, Obed Jackson Amoah, Hue Thi Nguyen, Jae Kyung Sohng
Summary: This study successfully glucosylated isoeugenol and other monoterpene derivatives in Corynebacterium glutamicum using a glycosyltransferase from Bacillus lichenformis. The cultivation conditions were optimized to achieve efficient glucosylation, and about 93% of isoeugenol was converted to glucosylated compounds.
Letter
Chemistry, Organic
Dipesh Dhakal, Dimitris Kallifidas, Manyun Chen, Sofia Kokkaliari, Qi-Yin Chen, Valerie J. Paul, Yousong Ding, Hendrik Luesch
Summary: A subcluster of cytotoxic apratoxin A was isolated from an environmental DNA library and engineered for expression in Anabaena sp. PCC7120. By further engineering with a rhamnose-inducible promoter, a chiral building block necessary for the synthesis of apratoxin analogues was successfully produced, marking the first synthetic biology attempt for this type of polyketide fragment.
Article
Biotechnology & Applied Microbiology
Nguyen Huy Thuan, Vinay Bharadwaj Tatipamula, Nguyen Thanh Trung, Nguyen Van Giang
Summary: This paper describes a recombinant Escherichia coli-based co-culture engineering approach for the production of genkwanin from glucose. By optimizing the production conditions, the yield of genkwanin was significantly improved.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2023)
