Article
Medicine, Research & Experimental
Francesca Toia, Elena Lo Presti, Anna Barbara Di Stefano, Marta Di Simone, Marco Trapani, Anna Maria Corsale, Carmela Picone, Francesco Moschella, Francesco Dieli, Adriana Cordova, Serena Meraviglia
Summary: This study analysed the immunomodulatory properties of spheroids from adipose stem cells (SASCs) and compared them to adipose stem cells (ADSCs). SASCs showed stronger immunomodulatory abilities in heterologous experimental settings compared to ADSCs. SASCs also demonstrated greater anti-inflammatory properties.
Review
Emergency Medicine
Xin Yuan, Li Li, Haofan Liu, Jing Luo, Yongchao Zhao, Cheng Pan, Xue Zhang, Yuwen Chen, Maling Gou
Summary: This article reviews emerging strategies to improve the therapeutic efficacy of adipose-derived stem cells (ADSCs) in tissue regeneration. These strategies include advanced cell expansion technologies, enhancing regenerative functions of ADSCs, and improving cell delivery efficiency. Critical issues about the sources, effectiveness, and safety of ADSCs are also pointed out.
Review
Biochemistry & Molecular Biology
Anna Trzyna, Agnieszka Banas-Zabczyk
Summary: The secretome of ASCs, including exosomes, contains a variety of cytokines and non-coding RNAs that have therapeutic potential in a wide range of diseases. Utilizing ASC secretome as a cell-free therapy shows promise in overcoming limitations of cell-based therapies and improving treatment efficacy.
Review
Pharmacology & Pharmacy
Meng Liu, Jiasheng Chen, Nailong Cao, Weixin Zhao, Guo Gao, Ying Wang, Qiang Fu
Summary: Lower urinary tract dysfunction often requires tissue repair or replacement. Stem cell therapy, particularly using adipose-derived stem cells (ASCs), shows great promise for treating lower urinary tract dysfunction. ASCs have strong regenerative potential, multi-differentiation potential, and can be combined with biomaterial scaffolds for tissue engineering repair.
Article
Multidisciplinary Sciences
Maumita Bhattacharjee, Jorge L. Escobar Ivirico, Ho-Man Kan, Shiv Shah, Takayoshi Otsuka, Rosalie Bordett, Mohammed Barajaa, Naveen Nagiah, Rishikesh Pandey, Lakshmi S. Nair, Cato T. Laurencin
Summary: Current treatment strategies for osteoarthritis (OA) mainly focus on symptom management and have limited disease-modifying effects. However, there is growing interest in using adipose-derived stem cells (ADSCs) and biomimetic injectable hydrogels as cell delivery systems for OA treatment. In this study, an amnion membrane (AM) hydrogel was developed as a biomimetic injectable hydrogel that can self-assemble and retain stem cells at the target site. The study demonstrated that both the AM hydrogel and ADSCs possess anti-inflammatory and chondroprotective properties, and their combined use can promote cartilage tissue regeneration in a rat OA model.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell & Tissue Engineering
Naoki Ishiuchi, Ayumu Nakashima, Satoshi Maeda, Yoshie Miura, Kisho Miyasako, Kensuke Sasaki, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Naoki Nakao, Masataka Nagao, Takao Masaki
Summary: Fibrosis is a common histological feature in chronic organ injury, leading to organ failure. Adipose-derived mesenchymal stem cells (ASCs) have been studied as a therapeutic tool to prevent organ failure. This study compared the therapeutic effects of ASCs from superficial and deep layers and found that both types of ASCs are equally effective and safe for treating organ injury.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Veterinary Sciences
Huina Luo, Dongsheng Li, Zhisheng Chen, Bingyun Wang, Shengfeng Chen
Summary: This study established a standardized process for manufacturing canine adipose-derived MSCs and evaluated the safety and efficacy of these cells for veterinary clinical use, providing a foundation for future applications.
BMC VETERINARY RESEARCH
(2021)
Article
Cell & Tissue Engineering
Lindsey Alejandra Quintero Sierra, Reetuparna Biswas, Anita Conti, Alice Busato, Riccardo Ossanna, Nicola Zingaretti, Pier Camillo Parodi, Giamaica Conti, Michele Riccio, Andrea Sbarbati, Francesco De Francesco
Summary: Fat graft is widely used in plastic and reconstructive surgery. Mechanical emulsification of fat tissue can solve the problems associated with fat resorption rates and adverse effects. Hy-Tissue Nanofat product, enriched with adipose-derived stem cells, shows great potential in regenerative medicine.
CELL TRANSPLANTATION
(2023)
Review
Cell & Tissue Engineering
Shilpa Sharma, Sathish Muthu, Madhan Jeyaraman, Rajni Ranjan, Saurabh Kumar Jha
Summary: With advancements in tissue engineering and regenerative medicine, biological products, particularly adipose tissue, have gained attention for their regenerative and immunomodulatory potential. Progenitor cells within adipose tissue are crucial for regeneration, and the use of adipose tissue by-products shows promising results in clinical applications.
WORLD JOURNAL OF STEM CELLS
(2021)
Article
Ophthalmology
Jilu Jaffet, Aparna Mohanty, Induvahi Veernala, Swati Singh, Mohammad Javed Ali, Sayan Basu, Geeta K. Vemuganti, Vivek Singh
Summary: This study provides the first evidence of the presence of mesenchymal stem cell (MSC) population within human lacrimal glands (LGs), suggesting their potential role in maintaining a healthy microenvironment within normal LGs and repairing diseased LGs.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Cell Biology
Ruirui Zhu, Ye Feng, Ruirui Li, Kelong Wei, Yun Ma, Qingyou Liu, Deshun Shi, Jieping Huang
Summary: Adipose-derived stem cells (ASCs) are a valuable resource for regenerative medicine and research on fat deposition. However, the isolation procedure of ASCs needs to be standardized and the differences in their proliferation and adipogenic differentiation potential from different fat depots require further understanding. This study compared the efficiency of ASCs isolation methods, with explant culture method showing simplicity and lower cost compared to the complex and costly enzymatic treatment method. ASCs isolated by the explant culture method exhibited better proliferation and adipogenic differentiation potential, although slightly lower than those isolated by the enzymatic treatment method. ASCs isolated from visceral depot demonstrated higher proliferation and adipogenic differentiation potential.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Review
Cell & Tissue Engineering
Mahshid Saleh, Amir Ali Sohrabpour, Mohammad Reza Mehrabi, Iman Seyhoun, Amir Abbas Vaezi
Summary: Peptic ulcer is a common gastrointestinal disorder that can be challenging to treat, often requiring long-term use of medication. Stem cell therapy, particularly using adipose-derived mesenchymal stem cells, is a promising new approach for patients who do not respond to conventional treatments or experience recurring ulcers.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Pharmacology & Pharmacy
Edward J. Calabrese
Summary: This paper provides a detailed assessment of hormetic dose responses in adipose-derived stem cells (ADSCs) of animal models and humans, showing that hormetic dose responses are commonly reported for ADSCs and may have a determinant role in potential clinical applications.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Tihomir Georgiev-Hristov, Mariano Garcia-Arranz, Jacobo Trebol-Lopez, Paula Barba-Recreo, Damian Garcia-Olmo
Summary: Adipose-derived stem cells have clinically beneficial qualities and have been investigated in basic research and clinical studies. Allogeneic adipose-derived stem cells have recently been approved for treating complex perianal fistulas in adults with Crohn's disease. The current understanding of adipose stem cell harvest includes quantitative and qualitative differences that may be influenced by donor conditions and sites. This review summarizes the current knowledge on the topic and proposes optimal donor profiles for further applications and studies.
Review
Chemistry, Multidisciplinary
Yi Qin, Gaoran Ge, Peng Yang, Liangliang Wang, Yusen Qiao, Guoqing Pan, Huilin Yang, Jiaxiang Bai, Wenguo Cui, Dechun Geng
Summary: In the past decade, adipose-derived stem cells (ADSCs) have gained increasing attention in the field of regenerative medicine due to their accessibility, multipotency, and active paracrine activity. This review discusses the challenges in translating ADSC-based therapies to clinical settings and proposes novel strategies to overcome the limitations. It also provides an update on the advancements in ADSC-based therapies in various fields of regenerative medicine.
Review
Biotechnology & Applied Microbiology
Behnaz Bakhshandeh, Nika Ranjbar, Ardeshir Abbasi, Elahe Amiri, Ali Abedi, Mohammad-Reza Mehrabi, Zahra Dehghani, Cristian Pablo Pennisi
Summary: Tissue engineering is a cutting-edge discipline that combines cells, scaffolds, and induction factors to regenerate and repair damaged tissue. Understanding the effects of biochemical and biophysical factors on cell fate is crucial for improving the efficiency of tissue engineering approaches. Biophysical signals provide a robust set of stimuli to manipulate cell signaling pathways during tissue formation.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Guoqiang Ren, Qiuyue Peng, Jeppe Emmersen, Vladimir Zachar, Trine Fink, Simone R. Porsborg
Summary: Adipose-derived Stem cells (ASCs) are about to be used in large-scale clinical trials for wound healing. It is important to develop potency assays for advanced therapy medicinal products (ATMPs) to ensure product consistency. Through analyzing ASC-based ATMPs from different donors, we found that certain regulatory genes associated with extracellular matrix and angiogenesis could be used as markers to select superior ASC donors for treating chronic wounds.
Article
Cell & Tissue Engineering
Zongzhe Xuan, Qiuyue Peng, Thomas Larsen, Leonid Gurevich, Jesper de Claville Christiansen, Vladimir Zachar, Cristian Pablo Pennisi
Summary: In this study, gelatin methacrylate (GelMA)/alginate hydrogels were optimized for bioprinting three-dimensional (3D) smooth muscle cell (SMC) constructs. The group with the highest concentrations of GelMA and alginate exhibited the highest viscosity and stability, supporting high cell viability, proliferation, and differentiation towards a contractile SMC phenotype. The results suggest that adjusting the composition of GelMA/alginate hydrogels is an effective means of controlling the SMC phenotype and provides a promising approach for studying smooth muscle adaptation and therapies for urethral strictures.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Thomas Starch-Jensen, Rubens Spin-Neto, Pernille Veiss-Pedersen, Christer Dahlin, Niels Henrik Bruun, Trine Fink
Summary: This study aimed to test the hypothesis that there would be no difference in radiographic outcome between maxillary sinus floor augmentation (MSFA) with allogeneic adipose tissue-derived stem cells (ASCs) seeded on depro-teinized bovine bone mineral (DBBM) and excipient on DBBM. The results showed that the GV was larger in the test group at one month but larger in the control group at four months. BD increased with both treatments, and the control group had higher BD at four months. The study concluded that allogeneic ASCs seeded on DBBM with MSFA did not improve the radiographic outcome compared with DBBM alone.
JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY
(2023)
Correction
Cell Biology
Qiuyue Peng, Guoqiang Ren, Zongzhe Xuan, Martyna Duda, Cristian Pablo Pennisi, Simone Riis Porsborg, Trine Fink, Vladimir Zachar
Review
Materials Science, Multidisciplinary
N. Pien, H. Krzyslak, S. Shastry Kallaje, J. Van Meerssche, D. Mantovani, C. De Schauwer, P. Dubruel, S. Van Vlierberghe, C. P. Pennisi
Summary: Additive manufacturing technologies have revolutionized tissue engineering by enabling the fabrication of complex tissues with intricate geometries. This review discusses recent advances in tissue engineering fabrication methods, with a focus on hierarchically organized anisotropic tissues. The importance of considering anatomical structure and function in the design process is emphasized, along with the factors influencing fabrication strategy and the advantages and limitations of different methods.
APPLIED MATERIALS TODAY
(2023)
Article
Biotechnology & Applied Microbiology
Simone Riis Porsborg, Hubert Krzyslak, Malgorzata K. Pierchala, Vincent Trole, Konstantin Astafiev, Rasmus Lou-Moeller, Cristian Pablo Pennisi
Summary: Excessive skin scarring affects a large number of patients worldwide, and an effective treatment has yet to be found. This study demonstrates the potential of ultrasound for scar reduction by developing a device based on printable piezoelectric material. The device was used to treat human fibroblasts with ultrasound, resulting in a significant reduction in fibroblast growth and extracellular matrix deposition. The overall results suggest that ultrasound treatment could be a beneficial therapy for scar reduction.
BIOENGINEERING-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Zongzhe Xuan, Leonid Gurevich, Jesper de Claville Christiansen, Vladimir Zachar, Cristian Pablo Pennisi
Summary: In this study, the effects of cyclic mechanical stimulation on smooth muscle cell maturation were investigated using three-dimensional constructs. The results showed that methacrylate-modified surfaces supported stable adhesion of the 3D constructs and facilitated cyclic mechanical stimulation, resulting in increased expression of contractile markers. These effects were mediated by activation of the p38 MAPK pathway. These findings provide valuable insights into the role of mechanical signaling in maintaining the contractile phenotype of bladder smooth muscle cells and have important implications for future treatments of LUT diseases.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Oncology
Behnaz Bakhshandeh, Shokufeh Ghasemian Sorboni, Nika Ranjbar, Roham Deyhimfar, Maryam Sadat Abtahi, Mehrnaz Izady, Navid Kazemi, Atefeh Noori, Cristian Pablo Pennisi
Summary: Stem cells are influenced by biochemical and biophysical cues from the extracellular matrix and neighboring cells. Biomechanical forces play a crucial role in regulating stem cell behavior and fate through mechanotransduction. Recent studies have highlighted the importance of biomechanical signals in tissue regeneration and shown the existence of precise control mechanisms in stem cell niches.
EXPERIMENTAL CELL RESEARCH
(2023)