期刊
ANGIOGENESIS
卷 21, 期 4, 页码 849-860出版社
SPRINGER
DOI: 10.1007/s10456-018-9630-9
关键词
MiR-153; Angiopoietin 1; Endothelial cell; Tumor angiogenesis; Breast cancer
资金
- National Natural Science Foundation of China [81660438, U1602221, 31771516, U1502222, 81772847, 81672639]
- Yunnan Applied Basic Research Key Project [2015FA027]
The sprouting of endothelial cells is the first step of tumor angiogenesis. Our previous study suggests that miR-153 suppresses breast tumor angiogenesis partially through targeting hypoxia-induced factor (HIF1). In this study, we demonstrated that miR-153 also suppresses the migration and the tube formation of endothelial cells through directly targeting angiopoietin 1 (ANG1) in breast cancer cells. There was a negative correlation between miR-153 and ANG1 levels in breast cancer. miR-153 blocked the expression and secretion of ANG1 in breast cancer cells through binding to ANG1 mRNA. Conditioned medium from the breast cancer cell, MCF7, treated with miR-153 had no effect on the proliferation of HUVECs, but significantly inhibited the migration and tube formation of HUVECs, which could be rescued by overexpression of ANG1. In addition, miR-153 also directly inhibited the proliferation and migration of MCF7 through downregulation of ANG1. These findings suggest that miR-153 suppresses the activity of tumor cells and the migration and tube formation of endothelial cells by silencing ANG1.
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