期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 17, 页码 4702-4706出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201801999
关键词
fluorescent probes; folding and unfolding; G-quadruplexes; live-cell imaging; RNA structures
资金
- Guangdong Provincial Key Laboratory of Construction Foundation [2011A060901014]
- National Natural Science Foundation of China [21672268, 81330077]
- Natural Science Foundation of Guangdong Province [2017A030308003, 2015A030306004]
Because of the absence of methods for tracking RNA G-quadruplex dynamics, especially the folding and unfolding of this attractive structure in live cells, understanding of the biological roles of RNA G-quadruplexes is so far limited. Herein, we report a new red-emitting fluorescent probe, QUMA-1, for the selective, continuous, and real-time visualization of RNA G-quadruplexes in live cells. The applications of QUMA-1 in several previously intractable applications, including live-cell imaging of the dynamic folding, unfolding, and movement of RNA G-quadruplexes and the visualization of the unwinding of RNA G-quadruplexes by RNA helicase have been demonstrated. Notably, our real-time results revealed the complexity of the dynamics of RNA G-quadruplexes in live cells. We anticipate that the further application of QUMA-1 in combination with appropriate biological and imaging methods to explore the dynamics of RNA G-quadruplexes will uncover more information about the biological roles of RNA G-quadruplexes.
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