Article
Engineering, Biomedical
Tania L. Lopez-Silva, Carlo D. Cristobal, Cheuk Sun Edwin Lai, Viridiana Leyva-Aranda, Hyun Kyoung Lee, Jeffrey D. Hartgerink
Summary: The study demonstrates that MDPs enhance neurite outgrowth and promote a multicellular pro-regenerative response in peripheral nerve injury.
Review
Biochemistry & Molecular Biology
Luke Juckett, Tiam Mana Saffari, Benjamin Ormseth, Jenna-Lynn Senger, Amy M. M. Moore
Summary: Peripheral nerve injuries (PNI) often result in lifelong disability with rare complete functional recovery. Intraoperative electrical stimulation (ES) is a promising surgical adjunct that can augment the intrinsic molecular pathways of nerve regeneration. Clinical trials have shown that ES can be an efficacious therapy to improve patient outcomes following PNIs.
Review
Cell Biology
Jingxin Zhang, Binjing Zhang, Jinglan Zhang, Weimin Lin, Shiwen Zhang
Summary: Magnesium as a suitable material for promoting peripheral nerve regeneration has both challenges and potentials, but there is currently limited research on this topic, requiring further exploration to address the shortcomings and challenges identified.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yangnan Hu, Zhuoyue Chen, Hongyang Wang, Jiahui Guo, Jiaying Cai, Xiaoyan Chen, Hao Wei, Jieyu Qi, Qiuju Wang, Huisheng Liu, Yuanjin Zhao, Renjie Chai
Summary: Inspired by the structures of peripheral nerves, researchers have developed a conductive topological scaffold for nerve repair using modified butterfly wings. The scaffold showed increased neurite length and guided cellular orientation, and successfully repaired nerve defects in rats.
Article
Engineering, Biomedical
Ping Wu, Zan Tong, Lihua Luo, Yanan Zhao, Feixiang Chen, Yinping Li, Celine Huselstein, Qifa Ye, Qingsong Ye, Yun Chen
Summary: The combination of VEGF and Schwann cells in peripheral nerve repair shows promising results in promoting nerve regeneration. The HSPS conduits loaded with VEGF-A overexpressing Schwann cells demonstrate high durability and efficiency in repairing nerve tissue, making them a potential new avenue for nerve tissue engineering.
BIOACTIVE MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Xiaolan Li, Xiang Zhang, Ming Hao, Dongxu Wang, Ziping Jiang, Liqun Sun, Yongjian Gao, Ye Jin, Peng Lei, Yue Zhuo
Summary: This review summarizes the importance of collagen in peripheral nerve regeneration, focusing on its characteristics, role, and optimization methods. Collagen, as a biomaterial, plays a crucial role in peripheral nerve repair and can be further optimized by adjusting its structure and physical properties, as well as combining it with bioactive components.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Simon C. Kellaway, Victoria Roberton, Joshua N. Jones, Rabea Loczenski, James B. Phillips, Lisa J. White
Summary: Incorporating dECMh derived from bone, liver, and small intestinal tissues can promote neurite extension and axonal regeneration, providing an alternative to current collagen I hydrogel.
ACTA BIOMATERIALIA
(2023)
Article
Cell Biology
Jung Il Lee, Ji Hun Park, Yeong-Rim Kim, Kihak Gwon, Hae Won Hwang, Gayoung Jung, Joo-Yup Lee, Jeong-Yun Sun, Jong Woong Park, Jae Ho Shin, Myoung-Ryul Ok
Summary: This study demonstrates the use of NO-releasing silica nanoparticles to enhance revascularization and improve nerve regeneration in a rat model of peripheral nerve injury, suggesting a potential therapeutic strategy for promoting functional recovery after nerve damage.
NEURAL REGENERATION RESEARCH
(2022)
Article
Chemistry, Applied
Sergey O. Solomevich, Carlo M. Oranges, Daniel F. Kalbermatten, Anna Schwendeman, Srinivas Madduri
Summary: Peripheral nerve repair is a serious problem in neurosurgery, with poor clinical outcomes and significant socioeconomic burden. Biodegradable polysaccharides show great potential for improving nerve regeneration. This review focuses on the promising therapeutic strategies involving different types of polysaccharides and their composites for promoting nerve regeneration, highlighting their applications in nerve guidance conduits, hydrogels, nanofibers, and films. The ease of therapeutic implementation, drug release properties, therapeutic outcomes, and future research directions are also discussed.
CARBOHYDRATE POLYMERS
(2023)
Review
Biotechnology & Applied Microbiology
Ane Escobar, Rui Luis Reis, Joaquim Miguel Oliveira
Summary: Peripheral nerve injuries are a common cause of disabilities, and treatment with long nerve gap autografts is currently considered the best method. However, due to limited availability and potential issues at the donor site, research is focusing on developing regenerative strategies using artificial nerve guidance conduits (NGCs). These NGCs can be optimized through various methods to enhance nerve regeneration, and recent advancements in using different types of nanoparticles to introduce growth factors to NGCs are discussed in depth.
Review
Biochemistry & Molecular Biology
Trevor Poitras, Douglas W. Zochodne
Summary: Common mechanisms of peripheral axon regeneration are recruited following diverse forms of damage to peripheral nerve axons. Supporting peripheral axon regrowth could be achieved through direct axon support or manipulation of the inflammatory milieu. Understanding the molecular barriers to regrowth and exploiting the downstream signaling pathways that impact neuron growth are promising approaches for promoting adult peripheral axon regeneration. This review emphasizes intrinsic aspects of adult peripheral axon regeneration and highlights several molecular barriers to regrowth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Kun Ye, Zitaiyu Li, Yinghao Yin, Jun Zhou, Dongjie Li, Yu Gan, Dongyi Peng, Ming Xiao, Liangyu Zhao, Yingbo Dai, Yuxin Tang
Summary: After stimulation with low-intensity pulsed ultrasound (LIPUS), Schwann cells (SCs) release exosomes (Exo) that can promote nerve regeneration and restore erectile function, improving the treatment outcome of neurogenic erectile dysfunction (NED).
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Biotechnology & Applied Microbiology
Lorena R. Lizarraga-Valderrama, Giulia Ronchi, Rinat Nigmatullin, Federica Fregnan, Pooja Basnett, Alexandra Paxinou, Stefano Geuna, Ipsita Roy
Summary: The study demonstrated that PHA-NGCs are highly promising in promoting peripheral nerve regeneration, showing comparable outcomes to autografts in functional evaluation and nerve morphology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Neurosciences
Supriya S. Wariyar, Alden D. Brown, Tina Tian, Tana S. Pottorf, Patricia J. Ward
Summary: This study investigates the impact of using fibrin glue in nerve repair on exercise-induced nerve regeneration. Results show that nerves repaired with fibrin glue exhibit more vasculature in the tissue bridge and have more robust axon regeneration after exercise compared to nerves without fibrin glue repair. When angiogenesis in the tissue bridge is prevented, exercise is unable to enhance regeneration, indicating the importance of a vascularized bridge. The combination of fibrin glue repair and exercise significantly improves vascular growth, axon elongation, neuromuscular junction reinnervation, and functional recovery.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Poornima Ramburrun, Pradeep Kumar, Elias Ndobe, Yahya E. Choonara
Summary: This study evaluated a biosimulated nerve repair system providing three types of regenerative cues in a peripheral nerve injury model. The nanofibers supported PC12 proliferation, preserved muscle mass, and gradually improved functional recovery. The combination of physical guidance, chemical cues, and therapeutic cues offers a promising strategy for the regeneration of severed peripheral nerves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sanya Sureram, Irene Arduino, Reiko Ueoka, Massimo Ritta, Rachele Francese, Rattanaporn Srivibool, Dhanushka Darshana, Jorn Piel, Somsak Ruchirawat, Luisa Muratori, David Lembo, Prasat Kittakoop, Manuela Donalisio
Summary: The peptide A-3302-B isolated from a marine bacterium, Micromonospora sp., strain MAG 9-7, showed specific inhibitory activity against HSV-2 by reducing cell-to-cell virus spread and transmission of extracellular free virus. It did not affect viral protein expression. The peptide also demonstrated anti-inflammatory activity and efficacy against an acyclovir-resistant HSV-2 strain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Benedetta Elena Fornasari, Federica Zen, Giulia Nato, Marco Fogli, Federico Luzzati, Giulia Ronchi, Stefania Raimondo, Giovanna Gambarotta
Summary: Angiogenesis plays a key role in nerve conduits by supporting cell survival and providing a pathway for the migration of newly formed Schwann cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Luisa Muratori, Federica Fregnan, Monica Maurina, Kirsten Haastert-Talini, Giulia Ronchi
Summary: Peripheral nerve injuries can lead to loss of motor and sensory function, and current methods for nerve regeneration have limited success. A potential approach for enhancing peripheral nerve regeneration is through improving diet and lifestyle, and consuming polyphenols.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Giacomo Carta, Benedetta Elena Fornasari, Federica Fregnan, Giulia Ronchi, Stefano De Zanet, Luisa Muratori, Giulia Nato, Marco Fogli, Giovanna Gambarotta, Stefano Geuna, Stefania Raimondo
Summary: This study found that neurodynamic treatment (NDT) promotes nerve regeneration, accelerates sensory and motor recovery, and modulates mechanical pain by affecting the expression of TACAN in the DRG.
Article
Biochemistry & Molecular Biology
Rui D. Alvites, Mariana V. Branquinho, Ana C. Sousa, Bruna Lopes, Patricia Sousa, Justina Prada, Isabel Pires, Giulia Ronchi, Stefania Raimondo, Ana L. Luis, Stefano Geuna, Artur Severo P. Varejao, Ana Colette Mauricio
Summary: Cell secretome is of great interest in Regenerative Medicine. This study analyzed the secretome collected from Olfactory Mucosa Mesenchymal Stem Cells and Olfactory Ensheathing Cells and applied it to promote peripheral nerve regeneration. The results showed that this therapeutic combination had beneficial effects on nerve and muscle regeneration, but further research is needed to address irregularities in the outcomes.
Review
Biochemistry & Molecular Biology
Giulia Ronchi, Federica Fregnan, Luisa Muratori, Giovanna Gambarotta, Stefania Raimondo
Summary: Regeneration of damaged peripheral nerves is a major challenge in neurosurgery and regenerative medicine. Various strategies, such as tissue-engineered nerve conduits and new interventions, are being developed to improve nerve functionality and patients' life quality. However, the diversity of evaluation methods and technologies across different labs makes it difficult to compare results. This review aims to discuss the usefulness and limitations of main morphological approaches used to evaluate nerve fiber regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Marwa El Soury, Oscar Dario Garcia-Garcia, Isabella Tarulli, Jesus Chato-Astrain, Isabelle Perroteau, Stefano Geuna, Stefania Raimondo, Giovanna Gambarotta, Victor Carriel
Summary: Hollow conduits have not been as successful as autografts in regenerating critical nerve gaps. Enriching conduits with extracellular materials and cells could improve their performance. In this study, the addition of fibrin-collagen hydrogels and adipose-derived mesenchymal stem cells showed promising results in early nerve regeneration.
NEURAL REGENERATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
David Munoz-Rodriguez, Marwane Bourqqia-Ramzi, Maria Teresa Garcia-Esteban, Antonio Murciano-Cespedosa, Alejandro Vian, Juan Lombardo-Hernandez, Pablo Garcia-Perez, Francisco Conejero, Alvaro Mateos Gonzalez, Stefano Geuna, Celia Herrera-Rincon
Summary: Inter-cellular communication involve biochemical, biophysical, and bioelectrical signals, which can occur between cells from the same tissue or animal species, as well as between cells that are evolutionarily distant. This paper presents a method for assessing the bioelectrical state of two bacterial strains and monitoring their bioelectrical profile during growth, revealing functional links between membrane potential and population growth. The study also demonstrates that bacteria respond to neural signals by changing their membrane potential, highlighting the potential for studying the relationship between bacteria and the brain in the context of the gut microbiota-brain axis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Yuki Shirosaki, Federica Fregnan, Luisa Muratori, Saki Yasutomi, Stefano Geuna, Stefania Raimondo
Summary: In this study, porous chitosan-GPTMS hybrids with different amounts of GPTMS were prepared. The structures of the degradation products with Si from the hybrids were analyzed using mass spectrometry, and their potential use in nerve repair devices was evaluated through biological assessments. The results showed that lower-molecular-weight degradation products with Si improved motor cell line differentiation and neurite outgrowth of dorsal root ganglion explants.
Review
Biochemistry & Molecular Biology
Patricia Sousa, Bruna Lopes, Ana Catarina Sousa, Alicia Moreira, Andre Coelho, Rui Alvites, Nuno Alves, Stefano Geuna, Ana Colette Mauricio
Summary: This article presents a systematic review of studies on the use of exosomes for wound healing over the past five years. The review shows that exosome-based treatments can improve wound healing by promoting angiogenesis, reepithelization, collagen deposition, and reducing scar formation. However, further research is needed due to the variability in cell sources, biomaterials, and administration routes.
Meeting Abstract
Cell & Tissue Engineering
Oscar-Dario Garcia-Garcia, David Sanchez-Porras, Marwa El-Soury, Jesus Chato-Astrain, Stefania Raimondo, Fernando Campos, Salvador Oyonarte, M. Carmen Sanchez-Quevedo, Stefano Geuna, Victor Carriel
TISSUE ENGINEERING PART A
(2022)