期刊
ANALYTICAL CHEMISTRY
卷 90, 期 4, 页码 2446-2450出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b04053
关键词
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资金
- National Science Foundation Division of Chemistry, under CAREER Award [CHE-1654274]
- Division of Molecular and Cellular Biosciences
- National Science Foundation Award [HRD-1547798]
In this work, nonlinear, stepping analytical mobility scan functions are implemented to increase the analytical separation and duty cycle during tandem Trapped Ion Mobility Spectrometry and FT-ICR MS operation. The differences between linear and stepping scan functions are described based on length of analysis, mobility scan rate, signal-to-noise, and mobility resolving power. Results showed that for the linear mobility scan function only a small fraction of the scan is sampled, resulting in the lowest duty cycle 0.5% and longest experiment times. Implementing nonlinear targeted scan functions for analysis of known mobilities resulted in increased duty cycle (0.85%) and resolving powers (R up to 300) broad with a 6-fold reduction in time from 30 to 5 min. For range characterization, a nonlinear mobility stepping scan function cycle provided the best sensitivity, resolving power, duty (4%), and points per peak. The applicability of nonlinear mobility is scan functions for the analysis of complex mixtures illustrated for the case of a direct infusion of a MCF-7 breast cancer cell digest, where isobaric peptides (e.g., DFTPAELR and TTILQSTGK) were separated in the mobility domain (Rims: 110) and identified based on their CCS, accurate mass (R-MS : 550k), and tandem MS using IRMPD in the ICR cell.
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