Article
Chemistry, Multidisciplinary
Yun Cui, Xuefang Dong, Xiaofei Zhang, Cheng Chen, Dongmei Fu, Xiuling Li, Xinmiao Liang
Summary: In this study, a comprehensive analysis of O-glycosylation of HKU1 S was conducted using dual-functional histidine-bonded silica (HBS) materials. A total of 46 novel O-glycosylation sites were identified on the HKU1 S1 subunit, with 55.6% predicted to be exposed on the outer protein surface. The obtained O-glycosylation dataset will provide valuable insights into the structure of HKU1 S.
FRONTIERS IN CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Shuaishuai Wang, Congcong Chen, Madhusudhan Reddy Gadi, Varma Saikam, Ding Liu, He Zhu, Roni Bollag, Kebin Liu, Xi Chen, Fengshan Wang, Peng George Wang, Peixue Ling, Wanyi Guan, Lei Li
Summary: The authors present an efficient chemoenzymatic modular assembly strategy for constructing structurally diverse O-GalNAc glycans, which are essential in various biological and pathological processes. The synthesized glycans are used to generate a synthetic mucin O-glycan microarray, revealing binding specificities of glycan-binding proteins, and providing insights into the structure-activity relationships of O-GalNAc glycans.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Organic
Zhuojia Xu, Yaqi Deng, Zhumin Zhang, Wenjing Ma, Wanjin Li, Liuqing Wen, Tiehai Li
Summary: A diversity-oriented chemoenzymatic approach was used for the collective preparation of sulfated core 2 O-GalNAc glycans and their nonsulfated counterparts. A sulfated trisaccharide and a nonsulfated trisaccharide were chemically synthesized, then enzymatic extension was carried out using a panel of robust glycosyltransferases to achieve regioselective sialylation, resulting in the generation of 36 structurally well-defined O-GalNAc glycans.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Shuang Yue, Xiaotong Wang, Wei Ge, Jiajia Li, Chuanlai Yang, Zeyang Zhou, Peng Zhang, Xiaodong Yang, Wenjin Xiao, Shuang Yang
Summary: Mucin-type O-glycosylation is a crucial protein post-translational modification expressed abundantly on cell surface proteins. It plays various roles in cellular functions, such as protein structure, signal transduction, and immune response. Dysregulation of mucin O-glycosylation can impair mucosal protection against infection or immune evasion. Truncated O-glycosylation, known as Tn antigen or O-GalNAcylation, is upregulated in diseases including cancer, autoimmune disorders, neurodegenerative diseases, and IgA nephropathy. Analyzing and understanding O-GalNAcylation have important clinical implications, although it remains challenging due to the lack of reliable assays compared to N-glycosylation.
Article
Chemistry, Multidisciplinary
Caleb A. H. Jones, Nathan D. Schley
Summary: The cleavage of alkyl ethers by hydrosilylation is demonstrated with stability of O-aryl glycosides under iridium- and borane-catalyzed conditions. A cationic bis(phosphine)iridium complex catalyzes the selective 3-demethylation of various 2,3,4-tri-O-methyl pyranoses.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yao Chen, Feng Tang, Hongqiang Qin, Xuyang Yue, Yongzhan Nie, Wei Huang, Mingliang Ye
Summary: In this study, a reversible chemoenzymatic labeling approach was developed for selective enrichment of O-GlcNAc peptides from complex samples. This approach allows tag-free identification of enriched glycopeptides and provides a unique avenue for proteome-wide analysis of protein O-GlcNAcylation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Multidisciplinary Sciences
Conor J. Kearney, Stephin J. Vervoort, Kelly M. Ramsbottom, Izabela Todorovski, Emily J. Lelliott, Magnus Zethoven, Lizzy Pijpers, Ben P. Martin, Timothy Semple, Luciano Martelotto, Joseph A. Trapani, Ian A. Parish, Nichollas E. Scott, Jane Oliaro, Ricky W. Johnstone
Summary: SUGAR-seq is a novel method that enables simultaneous detection of N-linked glycosylation, extracellular epitopes, and the transcriptome at the single-cell level, providing insights into cellular differentiation states. Integrated analysis using SUGAR-seq and glycoproteome identified tumor-infiltrating T cells with unique surface glycan properties that reflect their epigenetic and functional state.
Article
Chemistry, Medicinal
Yongheng Rong, Xingyun Wang, Weian Mao, Fang Yuan, Min Chen, Shengjun Wang, Peng George Wang, Zhigang Wu, Yunjiao He, Yun Kong
Summary: Harnessing highly conserved peptides from the receptor binding domain (RBD) of the S protein to construct peptide-based inhibitors is an effective strategy against SARS-CoV-2. This study explores the effect of O-glycosylation on the inhibition abilities of these inhibitors. The results show that the presence of sialic acid residues enhances the inhibition capacities of these peptide-based inhibitors, providing new directions for inhibitor design.
ACS INFECTIOUS DISEASES
(2022)
Article
Chemistry, Medicinal
Yongheng Rong, Xingyun Wang, Weian Mao, Fang Yuan, Min Chen, Shengjun Wang, Peng George Wang, Zhigang Wu, Yunjiao He, Yun Kong
Summary: This study synthesized peptide-based inhibitors containing specific glycan structures, and found that the sialic acid residue on the terminal of short O-glycan structures can enhance the inhibition capacities of these peptide-based inhibitors. This finding may provide novel optimization directions for inhibitor design.
ACS INFECTIOUS DISEASES
(2022)
Article
Chemistry, Analytical
Miloslav Sanda, Lindsay Morrison, Radoslav Goldman
Summary: The outbreak of the Covid-19 pandemic has caused the current global health crisis, prompting the need for detailed studies on SARS-CoV-2 proteins to develop effective antiviral compounds and vaccines. The glycosylation of the spike (S) protein plays a significant role in its proteolytic activation, expanding the current knowledge in this area.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Siyuan Kong, Quanqing Zhang, Lujie Yang, Yuanyu Huang, Mingqi Liu, Guoquan Yan, Huanhuan Zhao, Mengxi Wu, Xiangmin Zhang, Pengyuan Yang, Weiqian Cao
Summary: A new enrichment strategy using a boronic acid-functionalized mesoporous graphene-silica composite is proposed for isolating intact glycopeptides, showing effectiveness and specificity in glycosylation analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Anja Bluemler, Harald Schwalbe, Alexander Heckel
Summary: In this study, a multi-cycle chemoenzymatic synthesis of modified RNA with simplified solid-phase handling was developed to overcome the size limitations of RNA synthesis. The successful introduction of light-controllable RNA nucleotides was confirmed, and the method allows for modifications in the RNA backbone and introductions of photocaged and photoswitchable nucleotides, as well as photocleavable strand breaks and fluorophores.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Edgar Gonzalez-Rodriguez, Mia Zol-Hanlon, Ganka Bineva-Todd, Andrea Marchesi, Mark Skehel, Keira E. Mahoney, Chloe Roustan, Annabel Borg, Lucia Di Vagno, Svend Kjaer, Antoni G. Wrobel, Donald J. Benton, Philipp Nawrath, Sabine L. Flitsch, Dhira Joshi, Andres Manuel Gonzalez-Ramirez, Katalin A. Wilkinson, Robert J. Wilkinson, Emma C. Wall, Ramon Hurtado-Guerrero, Stacy A. Malaker, Benjamin Schumann
Summary: The polybasic cleavage motif for the protease furin is a major factor in human viral transmission of SARS-CoV-2 spike. Variants of concern (VOCs) have extensive mutations in the region preceding this motif. Besides furin, spikes from these variants rely on other proteases such as TMPRSS2 for maturation. Glycans near the cleavage site have implications for proteolytic processing and the effects of variant-borne mutations.
ACS CENTRAL SCIENCE
(2023)
Review
Biochemical Research Methods
Yuanwei Xu, Hui Zhang
Summary: Protein glycosylation is a diverse and ubiquitous modification that provides important properties for biological processes. Despite its significance, the investigation of O-linked glycoproteome remains challenging, with mass spectrometry serving as an indispensable tool for research.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Wenjing Ma, Yaqi Deng, Zhuojia Xu, Xingbang Liu, Digantkumar G. Chapla, Kelley W. Moremen, Liuqing Wen, Tiehai Li
Summary: Glycosylation of proteins is a complex post-translational modification, and the accurate functions of glycoproteins are still ambiguous. This research presents a streamlined chemoenzymatic method for preparing complex glycopeptides by integrating hydrophobic tag-supported chemical synthesis and enzymatic glycosylations. The approach demonstrated the efficient preparation of various complex glycopeptides of biological importance.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)