期刊
ANALYTICAL CHEMISTRY
卷 90, 期 9, 页码 5896-5902出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b00724
关键词
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资金
- NSF [CHE1402753]
- Welch Foundation [F-1155]
- National Science Foundation Graduate Research Fellowship [DGE-1610403]
- European Union's Horizon research and innovation programme [686547]
We demonstrate a method for determining the collision cross-sections (CCSs) of protein ions based on the decay rate of the time-domain transient signal from an Orbitrap mass analyzer. Multiply charged ions of ubiquitin, cytochrome c, and myoglobin were generated by electrospray ionization of both denaturing solutions and ones with high salt content to preserve native-like structures. A linear relationship between the pressure in the Orbitrap analyzer and the transient decay rate was established and used to demonstrate that the signal decay is primarily due to ion-neutral collisions for protein ions across the entire working pressure range of the instrument. The CCSs measured in this study were compared with previously published CCS values measured by ion mobility mass spectrometry (IMS), and results from the two methods were found to differ by less than 7% for all charge states known to adopt single gas-phase conformations.
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