期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 410, 期 23, 页码 5871-5878出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-018-1201-9
关键词
Antifouling; Aptamer; Immunoglobulin E; Peptide; Electrochemical biosensor
资金
- Natural Science Foundation of Shandong Province of China [ZR2017BB009]
- National Natural Science Foundation of China [21422504, 21675093]
- Taishan Scholar Program of Shandong Province of China [ts20110829]
Accurate detection of protein biomarkers in complex media remains a challenge due to severe nonspecific adsorption and biofouling, and sensing interfaces that combine the high sensitivity and antifouling ability are highly desirable. Herein, an antifouling sensing interface capable of sensitively assaying immunoglobulin E (IgE) in biological samples was constructed. The sensing interface was fabricated through the self-assembly of a zwitterionic peptide and the IgE aptamer onto a macroporous Au substrate, which was electrochemically fabricated with the aid of multilayer polystyrene nanospheres self-assembled on glassy carbon electrode. Due to the huge surface area arising from porous morphology and high specificity of aptamer, the developed electrochemical biosensor exhibits ultrahigh sensitivity and selectivity towards IgE, with the linear range of 0.110 pg mL(-1), and a very low limit of detection down to 42 fg mL(-1). Interestingly, owing to the presence of the zwitterionic peptide, the biosensing interface can satisfyingly reduce the nonspecific adsorption and fouling effect. Consequently, the biosensor was successfully applied to detect IgE in complex biological samples, indicating great promise of this peptide-based sensing interface for antifouling assays.
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