期刊
ANALYTICA CHIMICA ACTA
卷 1040, 期 -, 页码 81-89出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.07.024
关键词
Multiplex detection; Microfluidic chip; Foodborne pathogen; Loop-mediated isothermal amplification; Mixed dye; Food safety
资金
- National Natural Science Foundation of China [81473018, 81502849]
- Jilin Province Science and Technology Development Plan Item, China [20170204003SF]
- Open Research Project of the State Key Laboratory of Industrial Control Technology, Zhejiang University, China [ICT170293]
Foodborne pathogen is the primary cause of foodborne disease outbreak. Given its great damage, a sensitive, simple and rapid detection method is demanded. Herein, we described a self-priming polydimethylsiloxane (PDMS)/paper hybrid microfluidic chip, termed SPH chip, with mixed-dye-loaded loop-mediated isothermal amplification (LAMP) for multiplex foodborne pathogens detection. Staphylococcus aureus (SA) and Vibrio parahaemolyticus (VP) were chosen to verify the novel method. Compared to other similar detection devices, the SPH chip required easier fabrication process, less operation steps and lower cost. Additionally, the reaction result, especially for the weak-positive reaction, could be judged more accurately and conveniently due to the use of mixed-dye. Without pre-enrichment of bacteria in the food contaminated sample, the limit of detection (LOD) reached down to 1000 CFU mL(-1) with high specific. Additionally, for fully exploiting the potential of SPH chip, a conceptual eight-channel detection chip was also developed. Overall, the reliable and excellent result demonstrated that the novel method had great potential to be applied in the wider range of pathogens detection or disease diagnose, especially in some resource-limited area. (C) 2018 Elsevier B.V. All rights reserved.
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