Review
Chemistry, Multidisciplinary
Yuyue Xu, Tao Wang, Zhu Chen, Lian Jin, Zuozhong Wu, Jinqu Yan, Xiaoni Zhao, Lei Cai, Yan Deng, Yuan Guo, Song Li, Nongyue He
Summary: Point-of-care nucleic acid testing (POCNAT) plays a crucial role in infectious disease outbreaks, offering rapid, simple, and automated detection of nucleic acid. Leveraging advancements in technology, novel experimental carriers like chips, cartridges, and papers have been employed for automated nucleic acid detection, marking significant progress in the field.
CHINESE CHEMICAL LETTERS
(2021)
Review
Biotechnology & Applied Microbiology
Ziyu He, Changsheng Liu, Zhongyu Li, Zhou Chu, Xiang Chen, Xupeng Chen, Yuan Guo
Summary: The outbreak of COVID-19 has highlighted the importance of quick and easy testing. Point-of-care testing (POCT) devices offer convenience, speed, and timely results, attracting strong interest from researchers. However, there are still challenges in the development of POCT devices, such as sample pretreatment, detection sensitivity, and specificity. The unique properties of nanomaterials, such as magnetic, optical, thermal, and electrically conductive features, are expected to address these deficiencies and improve the performance of POCT devices.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Immunology
Ilesh Jani, Trevor F. Peter
Summary: Testing programs for SARS-CoV-2 have relied on PCR laboratory tests and rapid antigen assays, but their limitations in cost and accessibility have contributed to inequity in COVID-19 testing. Low-cost point-of-care nucleic acid tests have the potential to address these issues and improve pandemic preparedness, as well as strengthen primary healthcare services.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Junyang Mei, Dandan Wang, Yiheng Zhang, Dan Wu, Jinhui Cui, Mingzhe Gan, Peifeng Liu
Summary: Point-of-care testing (POCT) is a preferred method for detecting infectious pathogens, including COVID-19 and certain cancers. However, the application of POCT is limited by issues like unintegrated nucleic acid extraction, open detection systems that increase the risk of cross-contamination, and dependence on expensive equipment and AC power supply. This paper reports a portable platform based on recombinase polymerase amplification (RPA) that can rapidly detect SARS-CoV-2 virus and H. pylori bacteria within 30 minutes, with a low limit of detection. The platform uses a battery-powered portable reader for on-chip amplification and fluorescence detection, and can test for multiple pathogens simultaneously.
Article
Materials Science, Paper & Wood
Ruihua Tang, Min Li, Xueyan Yan, Mingyue Xie, Li Na Liu, Zedong Li, Feng Xu
Summary: This study compared the physical and chemical properties of six widely used paper-based nucleic acid extraction (NAE) materials and evaluated their NAE efficiency. The results showed that pore uniformity, wet strength, porosity, and functional groups are key parameters affecting the extraction efficiency. FTA card demonstrated the best NAE performance with high concentration and purity. This study provides important insights for the development of cost-effective paper-based NAE materials for point-of-care testing applications in the future.
Article
Materials Science, Multidisciplinary
Georgios Chondrogiannis, Pedro Reu, Mahiar M. Hamedi
Summary: Nucleic acid amplification testing (NAAT) is the gold standard for infectious disease diagnostics, but it is mainly limited to centralized laboratories. Researchers have addressed the bottleneck issue of DNA extraction by immobilizing the enzyme ACP on nitrocellulose, enabling home-based kits and expanding the capabilities of home-testing beyond antigen tests.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Analytical
Dongxu Zhang, Runxin Gao, Shaolei Huang, Yulin Huang, Jianbin Zhang, Xiaosong Su, Shiyin Zhang, Shengxiang Ge, Jun Zhang, Ningshao Xia
Summary: In this study, a microfluidic chip is proposed for on-site and rapid nucleic acid detection. The system has the advantages of high integration, low cost, and potential for large-scale production. It can be used for wide range screening during the COVID-19 pandemic and shows promising clinical application prospects.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Biotechnology & Applied Microbiology
Jingwen Wang, Han Jiang, Leiming Pan, Xiuying Gu, Chaogeng Xiao, Pengpeng Liu, Yulong Tang, Jiehong Fang, Xiaoqian Li, Chenze Lu
Summary: As nucleic acid testing becomes crucial in various research fields, there is an increasing demand for rapid on-site testing methods. This review discusses recent advances in on-chip methods for sample preparation, amplification, and detection in the nucleic acid testing procedure. It covers integration strategies for sample preparation, various on-chip amplification methods, and evaluation of potential detection methods. The review also highlights the importance of improving the speed and integration of all steps onto a single chip for future developments in nucleic acid testing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Chemistry, Analytical
Riccarda Antiochia
Summary: This review summarizes the current state of paper-based biosensors (PBBs) used in the detection of COVID-19, specifically focusing on three categories for SARS-CoV-2 diagnostics. It highlights the characteristics, analytical performance, advantages and drawbacks of each type of biosensor compared to traditional methods, with the hope of assisting scientists in developing novel PBB platforms with enhanced performance to help contain the COVID-19 outbreak.
Article
Chemistry, Analytical
Zhen Qin, Zongjie Huang, Peng Pan, Yueyue Pan, Runze Zuo, Yu Sun, Xinyu Liu
Summary: The study introduces an easy-to-fabricate nitrocellulose paper plate coupled with a desktop scanner for efficient ELISA measurements, providing a higher protein immobilization efficiency compared to traditional paper platforms.
Article
Biophysics
Tinglei Ma, Suyue Ren, Yixiang Wang, Haihan Yu, Lin Li, Xu Li, Luqing Zhang, Jinghua Yu, Yan Zhang
Summary: In recent years, point-of-care testing (POCT) technology has shifted from laboratory determination to practical applications. This article highlights the latest advances and principal issues in the design and fabrication of paper-based bipolar electrode electrochemiluminescence (BPE-ECL) sensors widely used in the POCT field. It discusses the attractive physical and chemical properties of cellulose paper, approaches to enhance paper functionality, and the materials used for fabricating paper-based BPE-ECL sensors. The article also introduces methods to enhance BPE-ECL signal and improve detection accuracy, as well as the application of paper-based BPE-ECL sensors in various fields.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Biotechnology & Applied Microbiology
Yuan Jia, Hao Sun, Jinpeng Tian, Qiuming Song, Wenwei Zhang
Summary: Paper-based point-of-care tests have gained interest for their rapid, low-cost, and instrument-free detection capabilities. This review focuses on the development of paper-based POC devices for detecting SARS-CoV-2, discussing principles, performance, advantages, drawbacks, limitations, and prospects of existing devices in the field.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Immunology
Xi Mo, Xueliang Wang, Zhaoqin Zhu, Yuetian Yu, Dong Chang, Xinxin Zhang, Dong Li, Fenyong Sun, Lin Zhou, Jin Xu, Hong Zhang, Chunfang Gao, Ming Guan, Yanqun Xiao, Wenjuan Wu
Summary: In 2021, COVID-19 continues to circulate globally, but under the precise policy implementation of China's public health system, the epidemic was quickly controlled, and society and the economy have recovered. Nucleic acid detection of SARS-CoV-2 has played a crucial role in pandemic response, with the emergence of POCT technology providing a complementary test to conventional PCR batch tests, improving medical safety and quality services.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biophysics
Fan Li, Qinghua Ye, Moutong Chen, Baoqing Zhou, Jumei Zhang, Rui Pang, Liang Xue, Juan Wang, Haiyan Zeng, Shi Wu, Youxiong Zhang, Yu Ding, Qingping Wu
Summary: The study introduces the trans-cleavage activity of CRISPR/Cas12a into an electrochemical biosensor (ECRISPR) combined with recombinase-assisted amplification (RAA) to develop a cost-effective, specific, and ultrasensitive method for Listeria monocytogenes detection. The RAA-based E-CRISPR sensor can detect as low as 0.68 aM of genomic DNA and 26 cfu/mL of L. monocytogenes in pure cultures, demonstrating rapid and ultrasensitive detection capabilities in spiked and natural samples. This system shows no cross-reactivity with other non-target bacteria, making it a simple, highly sensitive, and accurate platform for L. monocytogenes detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Richa Pandey, Yang Lu, Erin M. McConnell, Enas Osman, Alexander Scott, Jimmy Gu, Todd Hoare, Leyla Soleymani, Yingfu Li
Summary: DNAzyme-based electrochemical biosensors exhibit excellent sensitivity and specificity for disease diagnosis. This review critically discusses the impact of incorporating DNAzymes in electrochemical biosensing, focusing on the recent advances in developing DNAzyme-based electrochemical biosensors for infectious diseases, cancer, and regulatory diseases. It also addresses the challenges in translating these technologies from labs to clinics and discusses strategies to enhance the performance of existing technologies to create point-of-care electrochemical DNAzyme biosensors.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Biomedical
Ahmad Safwan Ahmad Latfi, Sumit Pramanik, Chi Tat Poon, Ahmad Mohammed Gumel, Khin Wee Lai, Mohamad Suffian Mohamad Annuar, Belinda Pingguan-Murphy
JOURNAL OF BIOMATERIALS APPLICATIONS
(2019)
Article
Cell & Tissue Engineering
Hui Yin Nam, Belinda Pingguan-Murphy, Azlina Amir Abbas, Azhar Mahmood Merican, Tunku Kamarul
STEM CELLS INTERNATIONAL
(2019)
Article
Radiology, Nuclear Medicine & Medical Imaging
Liang Kim Meng, Azira Khalil, Muhamad Hanif Ahmad Nizar, Maryam Kamarun Nisham, Belinda Pingguan-Murphy, Yan Chai Hum, Maheza Irna Mohamad Salim, Khin Wee Lai
CURRENT MEDICAL IMAGING
(2019)
Review
Engineering, Chemical
Shahla Ataei, Pedram Azari, Aziz Hassan, Belinda Pingguan-Murphy, Rosiyah Yahya, Farina Muhamad
ADVANCES IN POLYMER TECHNOLOGY
(2020)
Article
Instruments & Instrumentation
Shahla Ataei, Aziz Hassan, Pedram Azari, Belinda Pingguan-Murphy, Rosiyah Yahya, Wan Jefrey Basirun, Nurshafiza Shahabudin
SMART MATERIALS AND STRUCTURES
(2020)
Editorial Material
Chemistry, Analytical
Jane Ru Choi, Kar Wey Yong
Review
Chemistry, Multidisciplinary
Jane Ru Choi
FRONTIERS IN CHEMISTRY
(2020)
Review
Cell Biology
Jane Ru Choi, Kar Wey Yong, Jean Yu Choi, Alistair C. Cowie
Review
Biochemical Research Methods
Jane Ru Choi
Review
Biochemistry & Molecular Biology
Kar Wey Yong, Jane Ru Choi, Jean Yu Choi, Alistair C. Cowie
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Radiology, Nuclear Medicine & Medical Imaging
Ching Wai Yong, Khin Wee Lai, Belinda Pingguan Murphy, Yan Chai Hum
Summary: MRI is useful for visualizing soft tissues in the knee joint and understanding the progression of osteoarthritis. A study compared 10 encoder-decoder-based CNNs in cartilage delineation, with UNetVanilla showing the best performance.
CURRENT MEDICAL IMAGING
(2021)
Article
Polymer Science
Ismail Rozila, Pedram Azari, Sha'ban Munirah, Wan Kamarul Zaman Wan Safwani, Belinda Pingguan-Murphy, Kien Hui Chua
Summary: The co-culture of human adipose-derived stem cells and human osteoblasts at a ratio of 2:1 on PCL-HA scaffolds showed the most positive osteogenic differentiation, with higher ALP activity, calcium deposition, and expression of osteogenic genes, indicating an early stage of osteogenic development. The combination of co-culture system and PCL-HA scaffolds has the potential to promote osteogenic differentiation and early bone formation.
Article
Biochemical Research Methods
Jane Ru Choi, Jeong Hyun Lee, Alec Xu, Kerryn Matthews, Shuyong Xie, Simon P. Duffy, Hongshen Ma
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)