期刊
ANALYTICA CHIMICA ACTA
卷 1024, 期 -, 页码 161-168出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.03.027
关键词
Myocardial infarction; Colorimetric assay; Creatine kinase; Gold nanopartides; Biological fluids
资金
- Ministry of Science and Technology of Taiwan [MOST 104-2113-M-110-003-MY3]
Creatine kinase, a key biomarker associated with many debilitating physiological conditions has seldom been detected in biological fluids using functionalized gold nanoparticles (GNPs). We have developed a method based on the aggregation of cysteamine (Cys) functionalized GNPs in presence of ATP for effective detection of creatine kinase (CK-MM). Positively charged Cys-GNPs (brick red color) aggregate in presence of negatively charged ATP (blue color) but the process is prevented when CK-MM is added to the solution. The analytical response to the concentration of CK-MM is linear (R-2 = 0.9850). The proposed method is selective in sensing the CK-MM for a range of 5.617 x 10(3) ng/ml, 0.5617 ng/ml. The limit of detection was found to be 0.569 ng/ml in solution and 0.553 ng/ml in human serum with high selectivity. (C) 2018 Published by Elsevier B.V.
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