期刊
ANALYST
卷 143, 期 1, 页码 323-331出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7an01683b
关键词
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资金
- National Natural Science Foundation of China [21675137, 21205102]
- Natural Science Foundation of Jiangsu Province [BK20161170]
- China Postdoctoral Science Special Foundation [2016T90504]
- China Postdoctoral Science Foundation [2015M580471]
- Jiangsu Planned Project for Postdoctoral Research Funds [1501087B]
- Program for Distinguished Talents of Six Domains in Jiangsu Province [2016-SWXY-060]
- Jiangsu 333 project of cultivation of high-level talents
- Qing Lan Project of Jiangsu Province
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Copper ions (Cu2+) play a critical role in biological processes and are directly involved in beta-amyloid peptide (A beta) aggregation, which is responsible for the occurrence and development of Alzheimer's disease (AD). Therefore, combined determination of Cu2+ and A beta in one analytical system is of great significance to understand the exact nature of the AD event. This work presents a novel ratiometric electrochemical biosensor for the dual determination of Cu2+ and A beta(1-42). This unique sensor is based on a 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS) and poly(diallyldimethylammonium chloride) (PDDA)-bi functionalized single-walled carbon nanotubes (ABTS-PDDA/CNTs) composite. The inclusion of ABTS not only enhanced the sensitivity, but it also acted as an inner reference molecule to improve detection accuracy. The specific recognition of Cu2+ was realized by neurokinin B (NKB) coatings on the ABTS-PDDA/CNTs surface to form a [Cu-II(NKB)(2)] complex with Cu2+. The ABTS-PDDA/CNTs-NKB modified electrode also displayed an excellent electrochemical response toward the A beta(1-42) monomer, when a certain amount of the A beta(1-42) monomer was added to Cu2+-contained PBS buffer, which was due to the release of Cu2+ from the [Cu-II(NKB)(2)] complex through A beta binding to Cu2+. Meanwhile, our work showed that Cu2+ bound A beta(1-42) was concentration-dependent. Consequently, the presented electrochemical approach was capable of quantifying two important biological species associated with AD by one single biosensor, with the detection limits of 0.04 mu M for Cu2+ and 0.5 ng mL(-1) for A beta(1-42), respectively. Finally, the ratiometric electrode was successfully applied for monitoring Cu2+ and A beta(1-42) variations in plasma and hippocampus of normal and AD rats.
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