Article
Medicine, Research & Experimental
Bo Xu, Jiaqi Fang, Jianguang Wang, Xuehan Jin, Shengfu Liu, Kaihang Song, Ping Wang, Junjian Liu, Shuhao Liu
Summary: This study investigated the protective effect of edaravone on blood spinal cord barrier (BSCB) disruption after spinal cord injury (SCI). The results showed that edaravone treatment promoted functional recovery, improved vascular damage, and up-regulated BSCB-associated proteins in rats with SCI. In vitro experiments also demonstrated that edaravone improved cell viability, restored intercellular junctions, and promoted angiogenic activities. The underlying mechanism may involve the improvement of autophagy and the phosphorylation of RIP1/RIP3/MLKL. Overall, edaravone can be a potential treatment option for SCI.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Cell Biology
Hai-Yang Liao, Zhi-Qiang Wang, Rui Ran, Kai-Sheng Zhou, Chun-Wei Ma, Hai-Hong Zhang
Summary: Autophagy is crucial in spinal cord injury, with activation having neuroprotective effects but also potentially leading to neuronal death and impaired functional recovery.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Yun Li, Zhuofan Lei, Rodney M. Ritzel, Junyun He, Hui Li, Harry M. C. Choi, Marta M. Lipinski, Junfang Wu
Summary: Autophagy plays a crucial role in post-spinal cord injury, as inhibiting autophagy can lead to pro-inflammatory activation in microglia and worse functional outcomes, while increasing autophagy can reduce inflammation and improve outcomes.
Article
Biochemistry & Molecular Biology
Chenyu Wu, Huanwen Chen, Rong Zhuang, Haojie Zhang, Yongli Wang, Xinli Hu, Yu Xu, Jiafeng Li, Yao Li, Xiangyang Wang, Hui Xu, Wenfei Ni, Kailiang Zhou
Summary: The study found that betulinic acid has a significant therapeutic effect on spinal cord injury by promoting autophagy and mitophagy to improve functional recovery.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Neurosciences
Jia-quan Lin, He Tian, Xiao-guang Zhao, Sen Lin, Dao-yong Li, Yuan-ye Liu, Chang Xu, Xi-fan Mei
Summary: The study demonstrates that zinc provides neuroprotection after SCI by regulating the NLRP3 inflammasome through autophagy and ubiquitination.
CNS NEUROSCIENCE & THERAPEUTICS
(2021)
Article
Pharmacology & Pharmacy
Qiong Zhang, Mingfu Liu, Haibin Nong, Yanan Zhang, Yiguang Bai, Pan Liu, Shaohui Zong, Gaofeng Zeng
Summary: TFHL has shown to improve motor function recovery and promote spinal cord tissue repair in rats with spinal cord injury (SCI). It reduces apoptosis and improves the functional status of neurons. TFHL also improves cell morphology, microstructure, and axonal regeneration of damaged spinal motor neurons, and promotes the secretion of neurotrophic factors. Furthermore, TFHL promotes autophagy and related protein expression both in vivo and in vitro.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Fan Jiang, Mingjie Xia, Yanan Zhang, Jie Chang, Jiang Cao, Zhongkai Zhang, Zhanyang Qian, Lei Yang
Summary: Activation of CB2R attenuates neuroinflammation by promoting NLRP3 clearance, thus facilitating microglial polarization and functional recovery post-SCI.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Bowen Wan, Cong Li, Ming Wang, Fanqi Kong, Qirui Ding, Chenliang Zhang, Hao Liu, Dingfei Qian, Wenlin Deng, Jian Chen, Pengyu Tang, Qian Wang, Shujie Zhao, Zheng Zhou, Tao Xu, Yifan Huang, Jun Gu, Jin Fan, Guoyong Yin
Summary: The study demonstrates the crucial role of GIT1 in promoting MECs clearance of myelin debris and stimulating angiogenesis, making it a promising target for accelerating SCI recovery.
Article
Neurosciences
Simon Schading, Maryam Seif, Tobias Leutritz, Markus Hupp, Armin Curt, Nikolaus Weiskopf, Patrick Freund
Summary: Short MRI acquisition time, high signal-to-noise ratio, and high reliability are crucial for image quality. This study aims to reduce acquisition time and accurately measure cervical cord area (CSA) using a synthetic T1 MRI (synT1-w) estimated from quantitative maps of proton density, longitudinal, and effective transverse relaxation rates. The CSA measurements based on synT1-w images showed high repeatability and negligible differences compared to MPRAGE. These results demonstrate the potential of synT1-w images in reducing scan time significantly for comprehensive spinal cord assessment.
Article
Pharmacology & Pharmacy
Sissi Dolci, Loris Mannino, Emanuela Bottani, Alessandra Campanelli, Marzia Di Chio, Stefania Zorzin, Giulia D'Arrigo, Alessia Amenta, Agnese Segala, Giuseppe Paglia, Vanna Denti, Guido Fumagalli, Enzo Nisoli, Alessandra Valerio, Claudia Verderio, Giuseppe Martano, Francesco Bifari, Ilaria Decimo
Summary: Rewiring cellular metabolism to recover oxidative metabolism may be an innovative therapeutic strategy for the treatment of spinal cord injury (SCI). In this study, oral administration of metabolic precursors enhanced spinal cord oxidative metabolism, activated the mTORC1 anabolic pathway, increased mitochondrial mass and ROS defense, effectively preventing neural cell apoptosis. These molecular and histological modifications ultimately led to a significant improvement in motor functions.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Neuroimaging
Andreas Hug, Adriano Bernini, Haili Wang, Antoine Lutti, Johann M. E. Jende, Markus Boettinger, Marc-Andre Weber, Norbert Weidner, Simone Lang
Summary: The study used MRI to analyze chronic complete SCI patients and healthy individuals, revealing a volume reduction in the dorsal aspect of the brain in SCI patients, which correlated with spinal cord atrophy, suggesting Wallerian degeneration as a main contributor to observed spinal cord atrophy. However, structural changes in other brain regions were not confirmed.
NEUROIMAGE-CLINICAL
(2021)
Article
Cell Biology
Xin Xu, Chun-Jia Zhang, Zuliyaer Talifu, Wu-Bo Liu, Ze-Hui Li, Xiao-Xin Wang, Hua-Yong Du, Han Ke, De-Gang Yang, Feng Gao, Liang-Jie Du, Yan Yu, Ying-Li Jing, Jian-Jun Li
Summary: This study demonstrated the therapeutic effect of GlyNAC on spinal cord injury (SCI). GlyNAC promoted the recovery of motor and sensory functions in rats with SCI, protected spinal cord tissue, and delayed skeletal muscle atrophy.
Article
Immunology
Erliang Li, Rongbao Yan, Kang Yan, Rui Zhang, Qian Zhang, Peng Zou, Huimei Wang, Huan Qiao, Shuang Li, Qiong Ma, Bo Liao
Summary: We used single-cell RNA sequencing to study the cell types and signaling pathways related to immune autophagy in the site of spinal cord injury. Our data set provides valuable insights into the pathobiology of spinal cord injury and other traumatic diseases of the central nervous system.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Zili He, Jiqing Du, Yu Zhang, Yitie Xu, Qian Huang, Qingwei Zhou, Min Wu, Yao Li, Xie Zhang, Hongyu Zhang, Yuepiao Cai, Keyong Ye, Xiangyang Wang, Yingze Zhang, Qi Han, Jian Xiao
Summary: Increasing evidence suggests that defects in autophagy and autophagy-lysosomal pathway (ALP) may contribute to endothelial barrier disruption following acute traumatic spinal cord injury (SCI). This study investigates whether Kruppel-like factor 2 (KLF2) coordinates ALP in endothelial cells in SCI. The results demonstrate that KLF2 is a key contributor to SCI-mediated ALP dysfunction and blood-spinal cord barrier (BSCB) disruption, and could be a promising pharmacological target for SCI management and treatment.
Article
Biochemistry & Molecular Biology
Baokun Zhang, Fangqi Lin, Jiqing Dong, Jingwen Liu, Zhenyu Ding, Jianguang Xu
Summary: This study demonstrates that PM-Exos can promote recovery in spinal cord injury patients by activating microglial autophagy and enhancing the polarization of anti-inflammatory type microglia, potentially through inhibition of the PI3K/AKT/mTOR signaling pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Endocrinology & Metabolism
Carolina Nylen, Leonidas S. Lundell, Julie Massart, Juleen R. Zierath, Erik Naslund
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2019)
Article
Cell Biology
Ahmed M. Abdelmoez, Laura Sardon Puig, Jonathon A. B. Smith, Brendan M. Gabriel, Mladen Savikj, Lucile Dollet, Alexander Chibalin, Anna Krook, Juleen R. Zierath, Nicolas J. Pillon
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2020)
Review
Endocrinology & Metabolism
Mladen Savikj, Juleen R. Zierath
Article
Multidisciplinary Sciences
Leonidas S. Lundell, Evelyn B. Parr, Brooke L. Devlin, Lars R. Ingerslev, Ali Altintas, Shogo Sato, Paolo Sassone-Corsi, Romain Barres, Juleen R. Zierath, John A. Hawley
NATURE COMMUNICATIONS
(2020)
Review
Cell Biology
Sergej Pirkmajer, Metka Petric, Alexander Chibalin
Summary: AMP-activated protein kinase (AMPK) acts as a cellular energy gauge and regulator, playing a key role in maintaining cellular energy balance. It may also regulate the activity of Na+,K+-ATPase (NKA) in cells, which is essential for cellular functions. AMPK and NKA may interact in a complex manner to ensure proper energy utilization and cellular function under different conditions.
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
(2021)
Article
Endocrinology & Metabolism
Amy E. Mendham, Julia H. Goedecke, Yingxu Zeng, Steen Larsen, Cindy George, Jon Hauksson, Melony C. Fortuin-de Smidt, Alexander V. Chibalin, Tommy Olsson, Elin Chorell
Summary: The study found that improved mitochondrial respiration and content following aerobic and resistance training in women with obesity were not directly related to increased insulin sensitivity. Exercise training altered specific intramuscular lipid intermediates, associated with content-driven increases in mitochondrial respiration but not whole-body insulin sensitivity.
Article
Endocrinology & Metabolism
Jonathon A. B. Smith, Mladen Savikj, Parneet Sethi, Simon Platt, Brendan M. Gabriel, John A. Hawley, David Dunstan, Anna Krook, Juleen R. Zierath, Erik Naslund
Summary: Breaking sitting modestly increased activity behavior under free-living conditions, reducing fasting blood glucose levels and daily glucose variation. However, it was insufficient to modulate glucose tolerance or the skeletal muscle lipidome. Larger volumes of activity breaks may be required to promote greater health benefits.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Michael S. Kuefner, Erin Stephenson, Mladen Savikj, Heather S. Smallwood, Qingming Dong, Christine Payre, Gerard Lambeau, Edwards A. Park
Summary: The study shows that PLA2G2A enhances adipose tissue thermogenesis through increased substrate delivery and mitochondrial content in BAT, as demonstrated by the metabolic analysis and expression of UCP1 protein.
Article
Multidisciplinary Sciences
Nicolas J. Pillon, Jonathon A. B. Smith, Petter S. Alm, Alexander V. Chibalin, Julia Alhusen, Erik Arner, Piero Carninci, Tomas Fritz, Julia Otten, Tommy Olsson, Sophie van Doorslaer de ten Ryen, Louise Deldicque, Kenneth Caidahl, Harriet Wallberg-Henriksson, Anna Krook, Juleen R. Zierath
Summary: This study reveals the role of exercise-responsive cytokines in skeletal muscle development and growth in individuals with normal glucose tolerance or type 2 diabetes. Acute exercise triggers an inflammatory response and increases immune cell infiltration. CXCL12 is associated with skeletal muscle remodeling and differentiation.
Article
Biology
Nicolas J. Pillon, Laura Sardon Puig, Ali Altintas, Prasad G. Kamble, Salvador Casani-Galdon, Brendan M. Gabriel, Romain Barres, Ana Conesa, Alexander Chibalin, Erik Naslund, Anna Krook, Juleen R. Zierath
Summary: Obesity and elevated circulating lipids can disrupt the molecular circadian clock and impair metabolism. This study shows that lipid overload interacts with the circadian clock in skeletal muscle and alters the rhythmicity of gene expression through epigenomic mechanisms. Specific histone modifications are responsive to lipid overload and contribute to the reprogramming of circadian genes and pathways involved in lipid biosynthesis.
LIFE SCIENCE ALLIANCE
(2022)
Article
Genetics & Heredity
Farideh Moharrek, Lars R. Ingerslev, Ali Altintas, Leonidas Lundell, Ann N. Hansen, Lewin Small, Christopher T. Workman, Romain Barres
Summary: This study compared DNA methylation in spermatozoa from humans, mice, rats, and mini-pigs and found DNA methylation variation near genes related to the central nervous system and signal transduction. Additionally, gene expression dynamics during different preimplantation stages were modestly associated with spermatozoal DNA methylation at the nearest promoters.
Article
Endocrinology & Metabolism
Ahmed M. Abdelmoez, Oksana Dmytriyeva, Yasemin-Xiomara Zurke, Mette Trauelsen, Alesandra A. Marica, Mladen Savikj, Jonathon A. B. Smith, Claudia Monaco, Thue W. Schwartz, Anna Krook, Nicolas J. Pillon
Summary: Succinate released by skeletal muscle activates SUCNR1/GPR91, but it is unclear which cell types respond to succinate and the directionality of communication. This study found that SUCNR1 is expressed in macrophages but not in muscle cells, suggesting that the adaptive response of skeletal muscle to exercise is mediated via paracrine mechanisms involving macrophages.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Anna Nikolaevna Zakharova, Kseniya Gennadievna Milovanova, Anna Alekseevna Orlova, Elena Yuryevna Dyakova, Julia Gennadievna Kalinnikova, Olesya Vadimovna Kollantay, Igor Yurievich Shuvalov, Alexander Valerievich Chibalin, Leonid Vladimirovich Kapilevich
Summary: Type 2 diabetes mellitus is a significant problem, and the molecular mechanisms of glucose metabolism regulation are still insufficiently studied. Non-drug treatment, including physical activity, is relevant for metabolic disorders. This study aims to understand the effect of physical exercise on metabolic changes in mice with diabetes mellitus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physiology
Mladen Savikj, Emil Kostovski, Leonidas S. Lundell, Per O. Iversen, Julie Massart, Ulrika Widegren
PHYSIOLOGICAL REPORTS
(2019)
Review
Physiology
Minyu Zhang, Biyang Xiao, Xiaoqi Chen, Bingming Ou, Songtao Wang
Summary: Non-alcoholic fatty liver disease (NAFLD) is closely related to bile acid and gut microbiota disorders. Exercise plays an important role in maintaining the homeostasis of the enterohepatic circulation and the health of the host gut microbiota by regulating the composition and function of the bile acid pool in the enterohepatic axis. Exercise has been recommended as a first-line intervention for NAFLD, and regulating bile acids through exercise may be a promising treatment strategy.